LOCUS       MR227099                8256 bp    DNA     circular  SYN 
DEFINITION  Trpa1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1)
ACCESSION   NM_177781
VERSION     NM_177781.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="Trpa1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCGCGGC TTGAGGAGGA TTCTGCTCCC GGAGGAAAGG AAGGAGGTCC 
     1081 AGGGCGTTGT CTATCGCGGC GTCGGGGAAG ACATGGACTG CTCCAAGGAA TCCTTTAAGG 
     1141 TGGACATTGA AGGAGATATG TGTAGATTAG AAGACTTCAT CAAGAACCGA AGAAAACTAA 
     1201 GCAAATATGA GGATGAAAAT CTCTGTCCTC TGCATCACGC AGCAGCAGAA GGTCAAGTTG 
     1261 AACTGATGGA ACTGATCATC AATGGTTCTT CGTGTGAAGT GCTGAATATA ATGGATGGTT 
     1321 ATGGAAATAC CCCACTGCAT TGTGCTGCAG AAAAAAATCA AGTTGAAAGT GTAAAGTTTC 
     1381 TTCTCAGCCA AGGAGCAAAT CCAAACCTCC GAAATAGAAA CATGATGTCA CCCCTTCACA 
     1441 TAGCTGTGCA TGGCATGTAC AACGAAGTGA TCAAGGTGTT GACTGAGCAC AAGGCCACTA 
     1501 ACATCAATTT AGAAGGAGAG AATGGGAACA CGGCTTTGAT GTCCACGTGT GCCAAAGACA 
     1561 ACAGTGAAGC TTTGCAAATT TTGTTAGAAA AAGGAGCTAA GCTGTGTAAA TCAAATAAGT 
     1621 GGGGAGACTA CCCTGTGCAC CAGGCAGCAT TTTCAGGTGC CAAAAAATGC ATGGAATTAA 
     1681 TCTTAGCATA TGGTGAAAAG AACGGCTACA GCAGGGAGAC TCACATTAAT TTTGTGAATC 
     1741 ACAAGAAAGC CAGCCCTCTC CACCTAGCAG TTCAAAGCGG AGACTTGGAC ATGATTAAGA 
     1801 TGTGCCTGGA CAACGGTGCA CACATCGACA TGATGGAGAA TGCCAAATGC ATGGCCCTCC 
     1861 ATTTTGCTGC AACCCAGGGA GCCACTGACA TCGTTAAGCT CATGATCTCA TCCTATACCG 
     1921 GAAGTAGTGA TATTGTGAAT GCAGTTGATG GCAATCAGGA GACCCTGCTT CACAGAGCCT 
     1981 CGTTATTTGA TCACCATGAC CTGGCAGAAT ACCTAATATC AGTGGGAGCA GACATCAACA 
     2041 GCACTGATTC TGAAGGACGC TCTCCACTTA TTTTAGCAAC AGCTTCTGCA TCCTGGAACA 
     2101 TTGTGAATTT GCTCCTCTGT AAAGGTGCCA AAGTAGACAT AAAAGATCAT CTTGGACGTA 
     2161 ACTTTTTGCA TTTGACTGTG CAGCAGCCTT ATGGACTAAG AAATTTGCGG CCTGAGTTTA 
     2221 TGCAGATGCA ACACATCAAA GAGCTGGTGA TGGATGAAGA CAATGACGGA TGCACACCTC 
     2281 TCCATTATGC CTGTAGGCAG GGGGTTCCTG TCTCTGTAAA TAACCTCCTT GGCTTCAATG 
     2341 TGTCCATTCA TAGCAAAAGT AAAGATAAGA AGTCGCCCCT GCATTTTGCA GCCAGTTATG 
     2401 GGCGCATCAA TACATGTCAG AGACTTCTGC AAGACATAAG TGATACGAGG CTTTTGAATG 
     2461 AAGGGGATCT CCATGGGATG ACCCCTCTCC ACCTGGCAGC AAAAAATGGG CATGATAAAG 
     2521 TCGTTCAACT CCTTCTGAAG AAAGGGGCCT TATTTCTCAG TGACCACAAT GGCTGGACTG 
     2581 CTTTGCATCA CGCCTCCATG GGTGGGTACA CTCAGACCAT GAAGGTCATT CTTGATACTA 
     2641 ACTTGAAATG CACAGACCGA CTAGATGAAG AAGGGAACAC AGCACTCCAC TTTGCAGCAC 
     2701 GGGAAGGCCA TGCCAAGGCT GTTGCAATGC TTTTGAGCTA CAATGCTGAC ATCCTCCTGA 
     2761 ACAAGAAGCA AGCTTCCTTT CTGCATATTG CCCTGCACAA TAAGCGCAAG GAAGTGGTTC 
     2821 TCACAACCAT CAGAAATAAA AGATGGGATG AGTGTCTTCA AGTTTTCACT CATAATTCTC 
     2881 CAAGCAATCG ATGTCCAATC ATGGAGATGG TAGAATACCT CCCCGAGTGC ATGAAAGTTC 
     2941 TTTTAGATTT CTGCATGATA CCTTCCACAG AAGACAAGTC CTGTCAAGAC TACCATATTG 
     3001 AGTATAATTT CAAGTATCTC CAATGCCCAT TATCCATGAC CAAAAAAGTA GCACCTACCC 
     3061 AGGATGTGGT ATATGAGCCT CTTACAATCC TCAATGTCAT GGTCCAACAT AACCGCATAG 
     3121 AACTCCTCAA CCACCCTGTG TGTAGGGAGT ACTTACTCAT GAAATGGTGT GCCTATGGAT 
     3181 TCAGAGCCCA TATGATGAAC CTAGGATCTT ATTGTCTTGG TCTCATACCC ATGACCCTTC 
     3241 TTGTTGTCAA AATACAGCCT GGAATGGCCT TCAATTCTAC TGGAATAATC AATGGAACTA 
     3301 GTAGTACTCA TGAGGAAAGA ATAGACACTC TGAATTCATT TCCAATAAAA ATATGTATGA 
     3361 TTCTAGTTTT TTTATCAAGT ATATTTGGAT ATTGCAAAGA AGTGATCCAA ATTTTCCAAC 
     3421 AGAAAAGGAA TTACTTCCTG GATTACAACA ATGCTCTGGA ATGGGTTATC TATACAACTA 
     3481 GTATCATCTT CGTGTTGCCC TTGTTCCTCA ACATCCCAGC GTATATGCAG TGGCAATGTG 
     3541 GAGCAATAGC GATATTCTTC TACTGGATGA ACTTCCTACT GTATCTTCAA AGGTTTGAGA 
     3601 ACTGTGGAAT TTTCATTGTT ATGTTGGAGG TGATTTTTAA AACATTGCTG AGATCGACCG 
     3661 GAGTGTTTAT CTTCCTCCTA CTGGCTTTTG GCCTCAGCTT TTATGTTCTC CTGAATTTCC 
     3721 AAGATGCCTT CAGCACCCCA TTGCTTTCCT TAATCCAGAC ATTCAGTATG ATGCTAGGAG 
     3781 ACATCAATTA TCGAGATGCC TTCCTAGAAC CATTGTTTAG AAATGAGTTG GCATACCCAG 
     3841 TCCTGACCTT TGGGCAGCTT ATTGCCTTCA CAATGTTTGT CCCAATTGTT CTCATGAACT 
     3901 TACTGATTGG CTTGGCGGTT GGGGACATTG CTGAGGTCCA GAAGCATGCG TCATTGAAGA 
     3961 GGATTGCTAT GCAGGTGGAA CTTCATACCA ACTTAGAAAA AAAGCTGCCA CTCTGGTACT 
     4021 TACGCAAAGT GGATCAGAGG TCCACCATCG TGTATCCAAA TAGACCCAGG CACGGCAGGA 
     4081 TGCTACGGTT TTTTCATTAC TTTCTTAATA TGCAAGAAAC ACGACAAGAA GTACCAAACA 
     4141 TTGACACATG CTTGGAAATG GAAATATTGA AACAGAAATA TCGGCTGAAG GACCTCACTT 
     4201 CCCTCTTGGA AAAGCAGCAT GAGCTCATCA AACTCATCAT CCAGAAGATG GAGATCATCT 
     4261 CAGAGACAGA AGATGAAGAT AACCATTGCT CTTTCCAAGA CAGGTTCAAG AAGGAGAGGC 
     4321 TGGAACAGAT GCACAGCAAG TGGAATTTTG TCTTAAACGC AGTTAAGACT AAAACACATT 
     4381 GTTCTATTAG CCACCCGGAC TTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//