LOCUS       MR227050                8397 bp    DNA     circular  SYN 
DEFINITION  Itgax (Myc-DDK-tagged) - Mouse integrin alpha X (Itgax)
ACCESSION   NM_021334
VERSION     NM_021334.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4552..4643 
                     /label="Myc-DDK"  
     misc_feature    4673..5258 
                     /label="PolyA signal"  
     misc_feature    4730..4749 
                     /label="Restriction Enzyme"  
     misc_feature    5534..6206 
                     /label="ColE1"  
     misc_feature    6636..7430 
                     /label="Kan/Neo"  
     misc_feature    7465..7767 
                     /label="SV40 ori"  
     misc_feature    7853..8330 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4527..4534 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4527 
                     /label="Itgax"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCTGTACC TGGATAGCCT TTCTTCTGCT GTTGGGGTTT GTTTCTTGTC 
     1081 TTGGCTTCAA CTTGGATGCA GAGAAGCTGA CACATTTTCA CATGGACGGT GCTGAGTTCG 
     1141 GACACAGTGT GCTCCAGTAT GATAGTTCCT GGGTGGTGGT TGGAGCACCA AAGGAAATAA 
     1201 AAGCCACTAA CCAAATAGGT GGCCTCTACA AATGTGGCTA TCACACAGGC AACTGTGAGC 
     1261 CCATCTCCCT CCAGGTGCCC CCAGAGGCTG TGAACATATC CCTGGGCCTG TCCCTTGCTG 
     1321 CTGCCACCAA CCCTTCCTGG CTGTTGGCTT GTGGTCCTAC TGTGCACCAC ACATGCAGAG 
     1381 AGAATATATA CTTGACAGGG CTCTGCTTTC TACTGAGTTC ATCATTCAAG CAGAGCCAGA 
     1441 ACTTCCCAAC TGCACAGCAG GAGTGTCCAA AGCAAGACCA AGACATCGTG TTCCTGATTG 
     1501 ATGGCTCGGG TAGCATCAGT TCCACAGATT TTGAAAAAAT GCTGGACTTT GTTAAAGCTG 
     1561 TGATGAGCCA GCTTCAGAGA CCTAGCACAC GGTTCTCCCT GATGCAGTTC TCTGATTACT 
     1621 TCCGAGTACA TTTTACTTTC AACAACTTCA TCTCCACGTC AAGCCCTTTA AGTCTGTTGG 
     1681 GTTCTGTAAG GCAGCTAAGA GGGTACACAT ACACAGCCTC GGCTATCAAG CATGTCATAA 
     1741 CAGAACTGTT CACCACCCAA AGTGGAGCTC GGCAAGATGC CACCAAGGTC CTCATTGTCA 
     1801 TCACTGATGG GAGAAAACAA GGGGACAACT TGAGTTATGA TAGTGTCATC CCCATGGCAG 
     1861 AGGCTGCAAG CATCATTCGT TATGCAATTG GGGTAGGAAA GGCCTTTTAC AATGAACATT 
     1921 CCAAGCAAGA ATTAAAGGCC ATTGCATCGA TGCCTTCCCA TGAATACGTA TTCAGCGTGG 
     1981 AGAACTTTGA TGCTTTGAAG GATATTGAGA ATCAGCTGAA GGAGAAGATC TTTGCCATTG 
     2041 AGGGCACAGA GACACCAAGC AGCAGTACTT TTGAATTGGA GATGTCCCAG GAGGGCTTCA 
     2101 GTGCTGTGTT TACACCTGAT GGACCAGTTC TGGGGGCTGT GGGAAGCTTC AGCTGGTCTG 
     2161 GAGGTGCCTT CTTGTACCCC TCAAATATGA GACCCACCTT CATCAACATG TCTCAGGAGA 
     2221 ACGAGGACAT GAGGGACGCT TACCTGGGTT ACTCCACCGC ACTGGCCTTT TGGAAGGGGG 
     2281 TCCACAGCCT GATCCTGGGG GCCCCTCGCC ACCAGCACAC GGGGAAGGTT GTCATCTTTA 
     2341 CCCAGGAATC CAGGCACTGG AGGCCCAAGT CTGAAGTCAG AGGGACACAG ATCGGCTCCT 
     2401 ACTTTGGGGC ATCTCTTTGT TCTGTGGACA TGGATAGAGA TGGCAGCACT GACCTGGTCC 
     2461 TGATTGGAGT CCCCCATTAC TATGAGCACA CCCGAGGGGG GCAGGTGTCA GTGTGCCCCA 
     2521 TGCCCGGTGT GGGGAGCAGG TGGCATTGTG GGACCACCCT CCATGGGGAG CAGGGCCATC 
     2581 CTTGGGGCCG CTTTGGGGCG GCTCTGACAG TGCTAGGGGA CGTGAATGGG GACAGTCTGG 
     2641 CAGATGTGGC TATTGGTGCA CCCGGAGAGG AGGAGAACAG AGGTGCTGTC TACATATTTC 
     2701 ATGGAGCCTC AAGACAGGAC ATCGCTCCCT CACCCAGCCA GAGGATTTCA GCATCCCAGA 
     2761 TCCCCTCCAG GATTCAATAT TTTGGGCAGT CACTGAGTGG GGGCCAGGAC CTCACCCGGG 
     2821 ATGGACTGGT GGATCTGGCC GTGGGCTCCA AGGGACGCGT GCTGCTGCTC AGGACCAGAC 
     2881 CAATTCTTAG AGTGTCACCA ACTGTTCACT TCACACCTGC AGAGATTTCC AGGTCTGTGT 
     2941 TTGAGTGTCA GGAGCAGGTG GCCCCTGAGC AGACACTGAG TGATGCCACT GTCTGCCTTC 
     3001 ATATTCATGA AAGCCCCAAG ACCCAACTAG GTGACCTCCG AAGTACTGTC ACCTTTGACC 
     3061 TGGCTCTAGA CCATGGCCGT CTGAGTACCC GGGCCATCTT CAAGGAGACA AAGACCCGGG 
     3121 CTCTGACTCG AGTTAAAACT CTTGGTCTGA ACAAGCACTG TGAAAGTGTG AAGTTGCTTC 
     3181 TCCCGGCCTG TGTGGAGGAC TCAGTGACCC CGATCACTCT TCGCCTCAAC TTTTCTCTTG 
     3241 TTGGTGTGCC CATCAGTTCC TTACAAAATC TCCAACCCAT GCTGGCTGTA GATGACCAAA 
     3301 CGTACTTCAC GGCCTCTCTT CCCTTTGAGA AGAACTGTGG AGCTGACCAC ATCTGCCAGG 
     3361 ATGACCTTAG TGTCGTATTT GGCTTCCCAG ACTTGAAGAC CCTGGTGGTG GGAAGTGACC 
     3421 TGGAGCTGAA TGTAGATGTG ACGGTGTCTA ATGATGGAGA AGATTCCTAC GGAACCACAG 
     3481 TCACTCTGTT CTACCCCGTG GGTCTGTCCT TTAGACGAGT TGCAGAAGGC CAAGTATTCC 
     3541 TTCGGAAGAA AGAGGACCAG CAGTGGCAGC GGCGTGGGCA GCACTCCCTG CACCTCATGT 
     3601 GTGACAGCAC CCCAGACAGG AGTCAGGGCC TCTGGAGTAC CAGCTGTAGC AGCAGACATG 
     3661 TCATTTTCCG TGGAGGCTCA CAGATGACTT TTTTGGTAAC TTTTGACGTC TCCCCCAAAG 
     3721 CTGAGCTGGG GGACCGGCTG CTTCTAAGAG CCAGAGTGGG AAGTGAGAAT AATGTACCCG 
     3781 GAACCCCAAA AACCACCTTC CAGTTGGAGC TTCCAGTAAA ATATGCGGTG TACACCATGA 
     3841 TCAGCAGCCA TGACCAGTTT ACCAAGTATC TTAACTTCTC AACCTCAGAA AAGGAGAAGA 
     3901 CCAGTGTGGT CGAACATAGA TTCCAGGTGA ATAACCTGGG CCAGAGAGAC GTGCCAGTCA 
     3961 GCATCAACTT TTGGGTGCCC ATAGAGCTGA AGGGAGAAGC TGTGTGGACA GTGATGGTCT 
     4021 CCCACCCTCA GAACCCACTC ACCCAATGCT ATCGGAATAG ACTCAAGCCA ACACAATTTG 
     4081 ACCTCCTGAC TCACATGCAA AAGAGTCCCG TGCTGGACTG CTCCATCGCT GACTGCCTGC 
     4141 ACCTCCGCTG TGACATCCCC TCCTTGGGCA TCCTGGATGA GCTTTACTTC ATTCTGAAGG 
     4201 GCAACCTCAG CTTCGGCTGG ATCAGTCAGA CATTGCAGAA AAAGGTGTTG CTCCTGAGTG 
     4261 AGGCTGAAAT CACATTCAAC ACATCTGTGT ATTCCCAGCT GCCGGGACAG GAGGCATTTC 
     4321 TGAGAGCCCA GACGAAGACA GTGCTGGAGA TGTATAAAGT TCACAACCCC GTCCCTCTTA 
     4381 TCGTGGGCAG CTCAGTGGGA GGTCTGCTGC TGCTGGCTAT CATCACAGCT ATACTTTACA 
     4441 AGGCTGGTTT CTTCAAACGT CAGTACAAGG AGATGTTGGA GGAAGCAAAT GGACAGTTTG 
     4501 TCTCAGACGG GACCCCAACT CCCCAGGTTG CCCAGAGCGG ACCGACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//