LOCUS       MR227023                8214 bp    DNA     circular  SYN 
DEFINITION  Gli1 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI1 (Gli1)
ACCESSION   NM_010296
VERSION     NM_010296.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4371..4462 
                     /label="Myc-DDK"  
     misc_feature    4492..5077 
                     /label="PolyA signal"  
     misc_feature    4549..4568 
                     /label="Restriction Enzyme"  
     misc_feature    5353..6025 
                     /label="ColE1"  
     misc_feature    6455..7249 
                     /label="Kan/Neo"  
     misc_feature    7284..7586 
                     /label="SV40 ori"  
     misc_feature    7672..8149 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4353..4359 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4353 
                     /label="Gli1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAATCCA ATGACTCCAC CACAAGTCAA TAGCTATAGT GAGCCATGCT 
     1081 GTCTCCGACC CCTCCACAGC CAAGGAGTCC CCAGCATGGG AACAGAAGGA CTTTCTGGTC 
     1141 TGCCCTTTTG CCACCAAGCC AACTTTATGT CAGGGTCCCA GGGTTATGGA GCAGCCAGAG 
     1201 AGACCAGCAG CTGCACTGAA GGATCTCTCT TTCCTCCTCC TCCTCCTCCT CGGAGTTCAG 
     1261 TCAAATTAAC AAAGAAGCGG GCTCTCTCCA TCTCGCCCCT TTCTGATGCC AGCCTCGACC 
     1321 TGCAAACCGT AATCCGGACC TCACCCAGCT CCCTGGTGGC TTTCATCAAC TCTCGCTGTA 
     1381 CATCTCCGGG CGGTTCCTAC GGCCATCTCT CCATTGGTAC CATGAGCCCT TCTTTAGGAT 
     1441 TCCCACCTCA GATGAGTCAT CAAAAAGGAA CTTCACCTCC CTATGGAGTC CAGCCCTGTG 
     1501 TACCACATGA CTCTACTCGG GGTTCAATGA TGCTTCACCC CCAGTCCCGG GGACCACGTG 
     1561 CAACCTGCCA GCTGAAGTCA GAGCTGGATA TGATGGTTGG CAAGTGCCCG GAGGACCCTT 
     1621 TGGAAGGGGA CATGTCTAGC CCCAACTCCA CAGGCACACA GGATCACCTG TTGGGGATGC 
     1681 TGGATGGGCG GGAGGACCTG GAGAGAGAGG AGAAGCCTGA GCCTGAGTCT GTGTATGAGA 
     1741 CAGACTGCCG CTGGGATGGT TGCAGCCAGG AGTTCGATTC CCAGGAGCAG CTGGTGCACC 
     1801 ACATCAACAG TGAGCATATC CACGGGGAGC GGAAGGAATT CGTGTGCCAT TGGGGAGGTT 
     1861 GCTCCAGGGA GCTGAGGCCC TTCAAGGCCC AATACATGCT GGTGGTGCAC ATGCGCAGAC 
     1921 ACACGGGCGA GAAGCCACAC AAGTGCACGT TTGAAGGCTG TCGGAAGTCC TATTCACGCC 
     1981 TTGAAAACCT CAAGACGCAC CTTCGGTCGC ACACGGGTGA GAAGCCTTAC ATGTGTGAGC 
     2041 AAGAAGGTTG CAGCAAGGCC TTTAGCAATG CCAGTGACCG CGCCAAGCAC CAGAATCGGA 
     2101 CCCACTCCAA TGAGAAGCCA TACGTGTGCA AGCTCCCCGG CTGCACCAAG CGCTACACAG 
     2161 ATCCCAGCTC GCTCCGCAAA CACGTGAAGA CAGTGCATGG TCCGGATGCC CACGTGACCA 
     2221 AGCGGCATCG AGGGGATGGC CCCTTGCCAC GGGCTCAGCC CCTCTCCACA GTGGAGCCCA 
     2281 AGCGGGAAAG GGAAGGAGGA TCCGGCAGGG AAGAGAGCAG ACTGACTGTG CCCGAGAGTG 
     2341 CCATGCCGCA GCAGAGCCCC GGAGCGCAGT CCTCTTGCAG CAGCGACCAC TCCCCAGCAG 
     2401 GCAGTGCGGC CAACACGGAC AGCGGCGTGG AGATGGCCGG CAACGCCGGG GGCAGCACTG 
     2461 AGGACTTGTC CAGCTTGGAT GAAGGACCTT GTGTCTCGGC CACCGGACTC TCCACGCTTC 
     2521 GCCGCCTGGA GAACCTTAGG CTGGATCAGC TGCATCAGCT CCGGCCCATA GGGTCTCGGG 
     2581 GTCTCAAACT GCCCAGCTTA ACCCACGCTG GCGCACCTGT GTCTCGCCGT CTGGGCCCCC 
     2641 CAGTCTCCCT GGACCGCCGC AGCAGCAGCT CCAGCAGCAT GAGCTCTGCT TACACAGTCA 
     2701 GCCGCAGGTC CTCCCTGGCA TCCCCTTTCC CGCCGGGAAC CCCACCAGAG AATGGGGCAT 
     2761 CGTCACTACC TGGCCTCACA CCTGCTCAGC ACTACATGCT CCGTGCCAGA TATGCTTCAG 
     2821 CCAGGGGGAG TGGCACCCCG CCCACTGCAG CTCACAGCCT GGATCGGATG GGAGGTCTTT 
     2881 CTGTTCCTCC TTGGAGAAGC CGAACCGAGT ACCCGGGATA CAACCCAAAT GCAGGGGTCA 
     2941 CTCGGAGGGC CAGTGACCCA GCCCGGGCTG CTGACCACCC AGCTCCAGCC AGAGTCCAGC 
     3001 GGTTCAAGAG CCTGGGATGT GTCCACACGC CCCCTAGTGT GGCAACGGGA CGGAACTTCG 
     3061 ATCCCCACCA CCCTACCTCT GTCTATTCGC CACAGCCCCC CAGCATCACC GAAAATGTTG 
     3121 CCATGGATAC TAGGGGGCTA CAGGAGGAGC CAGAGGTTGG AACTTCTGTG ATGGGCAATG 
     3181 GTCTGAACCC ATACATGGAT TTTTCCTCCA CTGATACTCT GGGATATGGG GGACCCGAGG 
     3241 GGACGGCAGC TGAGCCTTAT GAAGCTAGGG GTCCAGGTTC CCTGCCTCTT GGGCCTGGTC 
     3301 CACCAACCAA CTATGGCCCT GGCCACTGTG CCCAGCAGGT CTCCTATCCT GATCCCACCC 
     3361 CAGAAAACTG GGGTGAGTTC CCTTCTCATG CTGGGGTGTA CCCTAGCAAT AAGGCTCCGG 
     3421 GTGCTGCCTA TAGCCAGTGT CCTCGACTTG AGCATTATGG ACAAGTGCAG GTAAAACCAG 
     3481 AACAAGGGTG CCCAGTGGGG TCTGACTCCA CCGGATTGGC ACCCTGCCTC AATGCCCACC 
     3541 CCAGTGAAGG GTCCCCAGGC CCGCAGCCTC TGTTTTCACA TCATCCCCAG CTCCCTCAGC 
     3601 CCCAGTATCC CCAGTCGGGT CCCTATCCTC AGCCTCCCCA TGGTTATCTC TCAACAGAAC 
     3661 CCAGGCTTGG CCTCAATTTC AACCCCTCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC 
     3721 AGCTGGTGTG TAATTACGTT CAGTCGCAGC AGGAATTGTT GTGGGAGGGA AGAAACCGGG 
     3781 GAGGGCTCCC CAACCAGGAA CTCCCATACC AGAGCCCCAA GTTTCTGGGG GGTTCCCAAG 
     3841 TTAGTCAGAG CCCTGCCAAG ACCCCAGCAG CAGCGGCGGC AGCATATGGA TCTGGCTTTG 
     3901 CACCTGCTTC GGCCAATCAC AAATCAGGCT CCTATCCTGC CCCTTCACCC TGCCATGAAA 
     3961 CTTTCACCGT GGGAGTAAAC AGGCCTTCCC ACAGGCCAGC AGCACCACCC CGACTTCTGC 
     4021 CCCCGCTGTC CCCTTGCTAT GGGCCCCTCA AGGTGGGGGA TACCAACCCC AGCTGTGGCC 
     4081 ATCCTGAGGT GGGCAGGTTA GGAGCAGGCC CTGCCTTGTA CCCTCCTCCT GAAGGGCAGG 
     4141 TGTGTAACGC TCTGGACTCT CTTGACCTGG ACAACACTCA GCTGGACTTT GTGGCTATCC 
     4201 TAGATGAGGC CCAGGGCCTG AGCCCTCCTC TTTCCCATGA GCAAGGGGAC AGCTCTAAAA 
     4261 ACACCCCATC TCCCTCTGGG CCCCCCAACA TGGCAGTGGG TAACATGAGT GTCTTGCTGG 
     4321 GGTCTCTGCC TGGAGAGACA CAATTCCTCA ACTCTAGTGC CACGCGTACG CGGCCGCTCG 
     4381 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4441 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4501 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4561 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4621 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4681 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4741 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4801 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4861 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4921 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4981 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5041 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5101 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5161 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5221 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5281 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5341 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5401 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5461 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5521 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5581 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5641 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5701 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5761 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5821 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5881 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5941 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6001 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6061 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6121 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6181 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6241 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6301 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6361 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6421 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6481 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6541 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6601 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6661 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6721 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6781 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6841 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6901 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6961 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7081 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7141 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7201 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7261 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7321 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7381 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7441 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7621 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7681 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7741 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7801 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7861 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7921 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7981 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8041 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8101 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8161 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//