LOCUS       MR226712                7071 bp    DNA     circular  SYN 
DEFINITION  Mmp9 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 9 (Mmp9)
ACCESSION   NM_013599
VERSION     NM_013599.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3228..3319 
                     /label="Myc-DDK"  
     misc_feature    3349..3934 
                     /label="PolyA signal"  
     misc_feature    3406..3425 
                     /label="Restriction Enzyme"  
     misc_feature    4210..4882 
                     /label="ColE1"  
     misc_feature    5312..6106 
                     /label="Kan/Neo"  
     misc_feature    6141..6443 
                     /label="SV40 ori"  
     misc_feature    6529..7006 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3210..3216 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3210 
                     /label="Mmp9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTCCCTGG CAGCCCCTGC TCCTGGCTCT CCTGGCTTTC GGCTGCAGCT 
     1081 CTGCTGCCCC TTACCAGCGC CAGCCGACTT TTGTGGTCTT CCCCAAAGAC CTGAAAACCT 
     1141 CCAACCTCAC GGACACCCAG CTGGCAGAGG CATACTTGTA CCGCTATGGT TACACCCGGG 
     1201 CCGCCCAGAT GATGGGAGAG AAGCAGTCTC TACGGCCGGC TTTGCTGATG CTTCAGAAGC 
     1261 AGCTCTCCCT GCCCCAGACT GGTGAGCTGG ACAGCCAGAC ACTAAAGGCC ATTCGAACAC 
     1321 CACGCTGTGG TGTCCCAGAC GTGGGTCGAT TCCAAACCTT CAAAGGCCTC AAGTGGGACC 
     1381 ATCATAACAT CACATACTGG ATCCAAAACT ACTCTGAAGA CTTGCCGCGA GACATGATCG 
     1441 ATGACGCCTT CGCGCGCGCC TTCGCGGTGT GGGGCGAGGT GGCACCCCTC ACCTTCACCC 
     1501 GCGTGTACGG ACCCGAAGCG GACATTGTCA TCCAGTTTGG TGTCGCGGAG CACGGAGACG 
     1561 GGTATCCCTT CGACGGCAAG GACGGCCTTC TGGCACACGC CTTTCCCCCT GGCGCCGGCG 
     1621 TTCAGGGAGA TGCCCATTTC GACGACGACG AGTTGTGGTC GCTGGGCAAA GGCGTCGTGA 
     1681 TCCCCACTTA CTATGGAAAC TCAAATGGTG CCCCATGTCA CTTTCCCTTC ACCTTCGAGG 
     1741 GACGCTCCTA TTCGGCCTGC ACCACAGACG GCCGCAACGA CGGCACGCCT TGGTGTAGCA 
     1801 CAACAGCTGA CTACGATAAG GACGGCAAAT TTGGTTTCTG CCCTAGTGAG AGACTCTACA 
     1861 CGGAGCACGG CAACGGAGAA GGCAAACCCT GTGTGTTCCC GTTCATCTTT GAGGGCCGCT 
     1921 CCTACTCTGC CTGCACCACT AAAGGCCGCT CGGATGGTTA CCGCTGGTGC GCCACCACAG 
     1981 CCAACTATGA CCAGGATAAA CTGTATGGCT TCTGCCCTAC CCGAGTGGAC GCGACCGTAG 
     2041 TTGGGGGCAA CTCGGCAGGA GAGCTGTGCG TCTTCCCCTT CGTCTTCCTG GGCAAGCAGT 
     2101 ACTCTTCCTG TACCAGCGAC GGCCGCAGGG ATGGGCGCCT CTGGTGTGCG ACCACATCGA 
     2161 ACTTCGACAC TGACAAGAAG TGGGGTTTCT GTCCAGACCA AGGGTACAGC CTGTTCCTGG 
     2221 TGGCAGCGCA CGAGTTCGGC CATGCACTGG GCTTAGATCA TTCCAGCGTG CCGGAAGCGC 
     2281 TCATGTACCC GCTGTATAGC TACCTCGAGG GCTTCCCTCT GAATAAAGAC GACATAGACG 
     2341 GCATCCAGTA TCTGTATGGT CGTGGCTCTA AGCCTGACCC AAGGCCTCCA GCCACCACCA 
     2401 CAACTGAACC ACAGCCGACA GCACCTCCCA CTATGTGTCC CACTATACCT CCCACGGCCT 
     2461 ATCCCACAGT GGGCCCCACG GTTGGCCCTA CAGGCGCCCC CTCACCTGGC CCCACAAGCA 
     2521 GCCCGTCACC TGGCCCTACA GGCGCCCCCT CACCTGGCCC TACAGCGCCC CCTACTGCGG 
     2581 GCTCTTCTGA GGCCTCTACA GAGTCTTTGA GTCCGGCAGA CAATCCTTGC AATGTGGATG 
     2641 TTTTTGATGC TATTGCTGAG ATCCAGGGCG CTCTGCATTT CTTCAAGGAC GGTTGGTACT 
     2701 GGAAGTTCCT GAATCATAGA GGAAGCCCAT TACAGGGCCC CTTCCTTACT GCCCGCACGT 
     2761 GGCCAGCCCT GCCTGCAACG CTGGACTCCG CCTTTGAGGA TCCGCAGACC AAGAGGGTTT 
     2821 TCTTCTTCTC TGGACGTCAA ATGTGGGTGT ACACAGGCAA GACCGTGCTG GGCCCCAGGA 
     2881 GTCTGGATAA GTTGGGTCTA GGCCCAGAGG TAACCCACGT CAGCGGGCTT CTCCCGCGTC 
     2941 GTCTCGGGAA GGCTCTGCTG TTCAGCAAGG GGCGTGTCTG GAGATTCGAC TTGAAGTCTC 
     3001 AGAAGGTGGA TCCCCAGAGC GTCATTCGCG TGGATAAGGA GTTCTCTGGT GTGCCCTGGA 
     3061 ACTCACACGA CATCTTCCAG TACCAAGACA AAGCCTATTT CTGCCATGGC AAATTCTTCT 
     3121 GGCGTGTGAG TTTCCAAAAT GAGGTGAACA AGGTGGACCA TGAGGTGAAC CAGGTGGACG 
     3181 ACGTGGGCTA CGTGACCTAC GACCTCCTGC AGTGCCCTAC GCGTACGCGG CCGCTCGAGC 
     3241 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3301 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3361 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3421 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3481 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3541 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3601 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3661 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3721 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3781 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3841 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3901 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3961 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4021 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4081 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4141 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4201 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4261 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4321 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4381 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4441 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4501 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4561 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4621 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4681 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4741 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4801 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4861 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4921 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4981 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5041 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5101 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5161 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5221 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5281 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5341 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5401 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5461 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5521 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5581 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5641 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5701 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5761 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5821 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5881 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5941 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6001 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6061 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6121 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6181 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6241 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6301 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6361 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6421 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6481 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6541 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6601 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6661 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6721 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6781 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6841 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6901 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6961 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7021 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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