LOCUS       MR226661                9222 bp    DNA     circular  SYN 
DEFINITION  Dcc (Myc-DDK-tagged) - Mouse deleted in colorectal carcinoma (Dcc)
ACCESSION   NM_007831
VERSION     NM_007831.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5379..5470 
                     /label="Myc-DDK"  
     misc_feature    5500..6085 
                     /label="PolyA signal"  
     misc_feature    5557..5576 
                     /label="Restriction Enzyme"  
     misc_feature    6361..7033 
                     /label="ColE1"  
     misc_feature    7463..8257 
                     /label="Kan/Neo"  
     misc_feature    8292..8594 
                     /label="SV40 ori"  
     misc_feature    8680..9157 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5361..5367 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5361 
                     /label="Dcc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAATAGT CTTGGATGTG TTTGGGTACC CAAGCTGGCT TTTGTACTCT 
     1081 TCGGAGCTTC CTTGCTCAGC GCACATCTTC AAGTCACGGG TTTCCAAATT AAACCTTTCA 
     1141 CGTCACTTCA TTTTGTGTCT GAACCTTCGG ATGCTGTCAC CATGCGGGGA GGAAATGTCC 
     1201 TGCTCAACTG CTCTGCAGAG TCCGACCGAG GAGTTCCAGT CATCAAGTGG AAGAAAGACG 
     1261 GCCTTATCCT AGCCTTGGGC ATGGATGACA GAAAGCAGCA ACTTCCTAAT GGATCTCTGT 
     1321 TGATACAAAA CATACTTCAT TCCAGACACC ATAAGCCGGA TGAAGGACTT TACCAATGCG 
     1381 AGGCATCCTT AGCAGATTCT GGCTCAATTA TTAGTCGGAC AGCAAAAGTT ACGGTAGCAG 
     1441 GGCCATTGAG GTTCCTTTCC CAGACGGAAT CTATCACAGC CTTCATGGGA GATACAGTGT 
     1501 TACTTAAGTG TGAAGTCATT GGGGAACCCA TGCCAACAAT ACACTGGCAG AAAAATCAAC 
     1561 AAGACCTCAA CCCACTCCCA GGAGACTCGC GTGTGGTCGT CTTGCCCTCC GGAGCATTGC 
     1621 AGATTAGCCG ACTTCAGCCA GGAGACAGTG GAGTCTACAG ATGCTCGGCT CGGAATCCAG 
     1681 CCAGCATAAG AACAGGAAAT GAAGCAGAAG TTAGAATTTT ATCAGACCCA GGCCTGCATA 
     1741 GGCAGCTATA TTTTCTGCAG AGACCATCCA ACGTAATAGC CATTGAAGGG AAAGATGCTG 
     1801 TCCTGGAGTG CTGTGTTTCT GGCTATCCTC CGCCAAGTTT CACCTGGTTA CGAGGCGAGG 
     1861 AGGTCATCCA ACTCAGGTCT AAAAAGTATT CATTGTTGGG TGGAAGCAAT TTGCTCATCT 
     1921 CTAATGTGAC AGATGATGAC AGTGGAACCT ATACTTGCGT TGTCACCTAT AAGAATGAGA 
     1981 ATATTAGTGC CTCTGCAGAG CTCACGGTCT TGGTTCCGCC CTGGTTTTTA AATCACCCTT 
     2041 CCAACCTCTA TGCCTATGAG AGCATGGATA TTGAGTTTGA ATGTGCAGTT TCTGGGAAGC 
     2101 CTGTGCCCAC TGTGAATTGG ATGAAGAATG GAGATGTGGT GATCCCGAGT GACTATTTTC 
     2161 AGATAGTGGG AGGAAGCAAC TTACGGATCC TTGGGGTGGT CAAGTCAGAT GAAGGCTTCT 
     2221 ATCAATGTGT GGCTGAAAAT GAGGCTGGCA ATGCCCAAAG TAGTGCCCAG CTGATTGTCC 
     2281 CCAAGCCTGC CATCCCAAGC TCCAGCATCC TCCCTTCTGC TCCCAGAGAT GTGCTCCCTG 
     2341 TCTTGGTTTC TAGTCGCTTT GTCCGTTTGA GCTGGCGTCC ACCTGCAGAA GCAAAAGGGA 
     2401 ACATTCAAAC CTTCACAGTC TTTTTCTCCA GAGAGGGTGA CAACAGGGAG CGAGCTTTGA 
     2461 ATACAACCCA GCCTGGGTCC CTTCAGCTTA CTGTGGGAAA CTTGAAACCA GAAGCCATGT 
     2521 ACACCTTCAG AGTTGTCGCC TACAATGAGT GGGGACCAGG GGAGAGTTCT CAGCCCATCA 
     2581 AGGTGGCCAC TCAGCCTGAG TTGCAAGTTC CAGGGCCAGT AGAAAACCTG CATGCTGTAT 
     2641 CTACCTCACC TACCTCAATT CTTATTACAT GGGAACCCCC TGCCTATGCA AATGGCCCAG 
     2701 TCCAAGGTTA CAGATTGTTC TGCACTGAGG TGTCTACAGG AAAAGAACAG AATATAGAGG 
     2761 TTGATGGACT ATCTTATAAA CTGGAAGGCC TGAAAAAATT CACGGAATAT ACTCTTCGAT 
     2821 TCCTAGCTTA TAATCGTTAT GGGCCAGGAG TATCTACTGA TGATATAACA GTTGTTACAC 
     2881 TTTCAGACGT GCCAAGTGCT CCACCTCAGA ACATCTCACT GGAAGTGGTT AACTCAAGGA 
     2941 GTATCAAAGT AAGCTGGCTC CCTCCTCCAT CGGGAACACA AAATGGATTT ATTACTGGCT 
     3001 ATAAAATTCG ACACAGAAAG ACTACCCGCA GGGGCGAGAT GGAAACACTG GAACCGAATA 
     3061 ACCTCTGGTA CCTCTTCACA GGATTGGAGA AAGGCAGTCA GTACAGTTTC CAAGTGTCGG 
     3121 CCATGACAGT CAATGGTACT GGACCACCTT CCAACTGGTA CACAGCGGAG ACCCCAGAGA 
     3181 ATGATTTAGA TGAGTCTCAA GTCCCTGACC AGCCGAGTTC TCTTCACGTG AGGCCCCAGA 
     3241 CAAACTGTAT CATCATGAGT TGGACACCTC CTCTCAACCC AAACATCGTG GTGAGAGGCT 
     3301 ATATTATTGG CTATGGTGTT GGCAGTCCTT ATGCTGAAAC AGTGCGTGTG GACAGTAAGC 
     3361 AGCGGTACTA TTCCATCGAG AGACTAGAGT CGAGTTCTCA TTATGTAATC TCCTTAAAAG 
     3421 CTTTCAACAA TGCCGGAGAA GGTGTCCCTC TCTACGAAAG TGCCACCACT CGTTCCATAA 
     3481 CAGATCCCAC TGACCCCGTT GATTATTATC CTTTGCTTGA TGATTTCCCC ACCTCGGGCC 
     3541 CAGATGTCTC CACCCCCATG CTCCCACCAG TAGGTGTACA GGCTGTGGCT CTTACCCATG 
     3601 AGGCTGTGAG GGTCAGCTGG GCAGACAACT CTGTCCCGAA GAACCAGAAA ACATCCGATG 
     3661 TGCGACTGTA CACCGTTCGG TGGAGGACCA GCTTTTCTGC CAGTGCCAAG TACAAGTCAG 
     3721 AAGACACAAC ATCTCTCAGT TACACAGCGA CTGGCCTCAA GCCCAATACC ATGTACGAGT 
     3781 TCTCAGTCAT GGTGACCAAG AACAGAAGGT CAAGCACGTG GAGCATGACT GCACACGCCA 
     3841 CCACCTACGA AGCTGCCCCA ACTTCTGCTC CCAAGGATCT GACAGTCATT ACTCGAGAGG 
     3901 GGAAGCCTCG TGCTGTCATT GTGAGCTGGC AGCCTCCCTT GGAAGCCAAT GGGAAAATCA 
     3961 CTGCTTATAT CTTGTTTTAT ACATTGGACA AGAATATACC AATAGATGAT TGGATTATGG 
     4021 AAACTATCAG CGGTGACCGG CTTACCCATC AGATCATGGA TCTCAGCCTT GACACGATGT 
     4081 ATTACTTTCG AATTCAAGCA CGGAATGTGA AAGGAGTAGG GCCCCTCTCT GATCCCATCC 
     4141 TCTTCAGGAC TTTGAAAGTG GAACACCCTG ACAAAATGGC TAATGACCAA GGTCGTCATG 
     4201 GAGATGGGGG TTATTGGCCA GTTGATACTA ACTTGATTGA TAGAAGCACT CTCAACGAAC 
     4261 CACCCATTGG ACAGATGCAC CCCCCGCATG GCAGTGTCAC TCCTCAGAAG AACAGCAACC 
     4321 TGCTTGTAAT AACCGTGGTC ACCGTTGGTG TTCTCACTGT GTTGGTCGTG GTGATTGTAG 
     4381 CAGTGATTTG CACCCGGCGT TCTTCAGCGC AGCAGAGGAA GAAACGGGCC ACACACAGTG 
     4441 TGAGCAAGAG GAAGGGCAGT CAGAAGGACC TCCGGCCTCC CGATCTTTGG ATACATCATG 
     4501 AAGAAATGGA AATGAAAAAT ATCGAGAAGC CTACGGGAAC CGACCCTGCA GGAAGAGACT 
     4561 CCCCCATCCA GAGCTGCCAA GATCTCACAC CAGTCAGCCA TAGCCAGTCA GAAACCCAGA 
     4621 TGGGAAGCAA AAGTGCCTCT CATTCAGGTC AGGACACTGA GGACGCAGGC AGCTCCATGT 
     4681 CCACTTTGGA ACGGTCCCTG GCAGCACGCC GGGCCACCAG GGCCAAGCTC ATGATTCCCA 
     4741 TGGAGGCCCA GTCCAGTAAT CCTGCTGTTG TGAGTGCCAT CCCTGTACCA ACACTAGAAA 
     4801 GTGCCCAGTA TCCAGGAATC CTCCCATCTC CCACATGTGG ATACCCGCAT CCACAGTTCA 
     4861 CTCTCCGGCC AGTACCATTC CCAACGCTGT CTGTGGACCG AGGTTTTGGA GCAGGAAGAA 
     4921 CTCAGTCTGT GAGTGAAGGA CCAACCACGC AACAGCAACC CATGCTGCCC CCAGCTCAGC 
     4981 CCGAACATCC GAGCAGTGAA GAAGCACCCA GTAGAACCAT CCCCACAGCC TGTGTGCGGC 
     5041 CAACTCATCC ACTCCGCAGC TTTGCTAACC CATTACTACC TCCACCCATG AGTGCAATAG 
     5101 AACCAAAAGT CCCTTACACA CCGCTTTTGT CTCAGCCAGG ACCCACCCTT CCCAAGACTC 
     5161 ATGTTAAAAC GGCCTCCCTT GGGCTGGCCG GAAAGGCAAG GTCCCCTTTG CTTCCCGTGT 
     5221 CTGTGCCAAC AGCCCCTGAA GTGTCTGAGG AGAGCCACAA GCCCACAGAG GATCCAGCCA 
     5281 GTGTATATGA ACAGGATGAC TTGAGCGAAC AAATGGCAAG TTTGGAAGGG CTAATGAAGC 
     5341 AACTCAACGC CATCACAGGG TCTGCCTTTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5401 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5461 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5521 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5581 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5641 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5701 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5761 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5821 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5881 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5941 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6001 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6061 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6121 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6181 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6241 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6301 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6361 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6421 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6481 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6541 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6601 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6661 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6721 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6781 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6841 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6901 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6961 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7021 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7081 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7141 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7201 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7261 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7321 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7381 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7441 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7501 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7561 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7621 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7681 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7741 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7801 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7861 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7921 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7981 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8041 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8101 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8161 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8221 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8281 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8341 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8401 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8461 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8521 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8581 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8641 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8701 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8761 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8821 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8881 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8941 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9001 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9061 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9121 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9181 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//