LOCUS       MR226638L3                8711 bp    DNA     circular  SYN 
DEFINITION  Ids (Myc-DDK-tagged) - Mouse iduronate 2-sulfatase (Ids), transcript variant 1
ACCESSION   NM_010498
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7002..7182 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7910..8569 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7230..7849 
                     /label="ColE1"  
     misc_feature    5042..5065 
                     /label="DDK"  
     misc_feature    5877..5897 
                     /label="LR50"  
     misc_feature    4994..5065 
                     /label="MYC"  
     misc_feature    5075..5140 
                     /label="P2A"  
     misc_feature    5141..5740 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    5886..6477 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4976..4982 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4976 
                     /label="Ids"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCGCCGCCG CCGCCGCCGC CTATCTGGCG 
     3361 GCAGCTTAGC TTCAGCTTGT TGCTAGGCTC CTTCTGCATC GCCCTGGAGT CCGCAGCGCA 
     3421 AGGCAACTCT GCTACAGATG CTCTGAACAT CCTTCTGATC ATTGTGGATG ATCTCCGCCC 
     3481 CTCCCTGGGC TGTTATGGAG ACAAGCTAGT GAGGTCCCCG AACATTGACC AGCTGGCATC 
     3541 CCATAGCGTT CTCTTTCAGA ATGCCTTTGC TCAGCAAGCA GTGTGCGCAC CAAGTCGAGT 
     3601 GTCCTTCCTC ACCGGGAGGA GGCCTGACAC CACCCGCCTG TATGACTTCA ATTCCTACTG 
     3661 GAGGGTACAT TCTGGAAATT TTTCCACCAT CCCCCAATAC TTCAAGGAGA ATGGCTACGT 
     3721 GACCATGTCG GTTGGGAAAG TCTTTCACCC TGGTATATCT TCCAATCACA GTGATGATTA 
     3781 TCCATATAGC TGGTCTTTTC CACCTTATCA TCCATCCTCA GAGAAATATG AAAACACTAA 
     3841 GACGTGTAAG GGACAAGATG GAAAACTCCA CGCCAATCTG CTTTGTCCTG TCGATGTGGC 
     3901 AGATGTGCCT GAAGGTACCT TGCCTGACAA ACAGAGCACA GAAGAAGCCA TCAGGTTGTT 
     3961 GGAAAAGATG AAGACATCAG CTAGTCCTTT CTTCCTGGCA GTTGGGTACC ACAAGCCGCA 
     4021 TATCCCCTTC AGATACCCCA AGGAATTTCA GAAGTTGTAT CCCTTGGAAA ACATAACCCT 
     4081 GGCTCCTGAT CCCCATGTTC CTGATAGCCT ACCACCTGTA GCCTACAACC CCTGGATGGA 
     4141 TATCAGGGAG AGGGAAGATG TCCAAGCCTT AAACATCAGT GTTCCCTATG GACCAATTCC 
     4201 TGAGGATTTT CAGCGGAAAA TCCGCCAGAG CTACTTTGCT TCTGTGTCCT ATTTGGATAC 
     4261 TCAGGTTGGA CATGTTTTGA GTGCTTTGGA TGATCTTCGT CTAGCCCACA ACACAATTAT 
     4321 TGCTTTCACA TCTGATCACG GATGGGCCCT AGGTGAACAC GGAGAATGGG CCAAGTACAG 
     4381 CAACTTTGAT GTTGCTACCC GTGTGCCCCT GATGCTCTAT GTCCCTGGAA GGACGGCCCC 
     4441 ACTTCCTGCG GCAGGCCAGA AGCTCTTTCC ATACCGGGAC CCCTTTGATC CGGCCTCGGA 
     4501 TTGGATGGAT GCAGGCAGGC ACACCGAGGA CCTCGTAGAA CTTGTATCTC TCTTCCCCAC 
     4561 GCTCGCTGGA CTTGCAGGAC TGCCAGTTCC TCCTCGGTGC CCCATCCCTT CTTTTCATGT 
     4621 TGAGCTTTGC AGAGAAGGCC AGAATCTTCA GAAGCATTTG CAGCTCCATG ACTTGGAAGA 
     4681 GGAGCCAGAC TTGTTTGGTA ATCCCCGGGA GTTGATCGCC TATAGCCAGT ACCCCCGGCC 
     4741 TGCAGATTTC CCTCAGTGGA ATTCTGACAA GCCAAGCTTA AATGATATAA AGGTCATGGG 
     4801 ATATTCTATA CGCACTGTAG ACTACAGGTA CACTGTGTGG GTTGGCTTCG ATCCTAGTGA 
     4861 ATTTCTGGCT AACTTTTCTG ATATCCATGC CGGGGAACTC TATTTTGTCG ATTCTGACCC 
     4921 ACTGCAGGAT CACAATGTAT ATAATGACTC CCAACATGGA GGTCTTCTCC ACTCACTGAG 
     4981 GCCTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     5041 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTGG GTAGGAAGCG GAGCTACTAA 
     5101 CTTCAGCCTG CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCTA TGACCGAGTA 
     5161 CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC 
     5221 CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGATC GCCACATCGA 
     5281 GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT 
     5341 GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC 
     5401 GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC 
     5461 CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT 
     5521 CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT 
     5581 GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC 
     5641 GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT 
     5701 GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAT GTACACCCAA 
     5761 ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC 
     5821 ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA 
     5881 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC 
     5941 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG 
     6001 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC 
     6061 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG 
     6121 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG 
     6181 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA 
     6241 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT 
     6301 TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG 
     6361 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC 
     6421 CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC 
     6481 CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC 
     6541 AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG 
     6601 GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT 
     6661 CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA 
     6721 CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC 
     6781 TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA 
     6841 CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC 
     6901 CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC 
     6961 AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC 
     7021 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG 
     7081 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT 
     7141 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA 
     7201 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 
     7261 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 
     7321 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 
     7381 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 
     7441 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 
     7501 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 
     7561 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 
     7621 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 
     7681 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 
     7741 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 
     7801 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 
     7861 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT 
     7921 ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT 
     7981 GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC 
     8041 CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA 
     8101 CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT 
     8161 CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT 
     8221 ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT 
     8281 CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC 
     8341 CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA 
     8401 TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT 
     8461 CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT 
     8521 CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC 
     8581 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8641 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8701 TGCCACCTGA C
//