LOCUS       MR226584                8259 bp    DNA     circular  SYN 
DEFINITION  Magi3 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 2
ACCESSION   NM_133853
VERSION     NM_133853.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4416..4507 
                     /label="Myc-DDK"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    4594..4613 
                     /label="Restriction Enzyme"  
     misc_feature    5398..6070 
                     /label="ColE1"  
     misc_feature    6500..7294 
                     /label="Kan/Neo"  
     misc_feature    7329..7631 
                     /label="SV40 ori"  
     misc_feature    7717..8194 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4398..4404 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4398 
                     /label="Magi3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAGACG TTGAAGAAGA AGAAGCACTG GCTCAGCAAG GTGCAGGAGT 
     1081 GTGCGGTATC CTGGGCCGGG CCCCCGGGCG ACTTGGGCGC CGAGATCCGC GGCGGCGCCG 
     1141 AGCGCGGCGA GTTCCCTTAC CTGGGGCGGC TCCGCGACGA GGCTGGCGGC GGCGGAGGCA 
     1201 CCTGCTGCGT GGTCTCGGGC AAGGCGCCCA GTCCGGGCGA TGTGCTGTTG GAGGTGAACG 
     1261 GGACGCCGGT CAGTGGGCTC ACCAACCGGG ACACTCTGGC TGTCATCCGC CACTTCCGCG 
     1321 AGCCCATCCG TCTGAAGACG GTGAAGCCAG GCAAAGTTAT AAATAAAGAT TTGCGACACT 
     1381 ACCTGAGTCT TCAGTTTCAG AAAGGATCAA TTGACCACAA ACTACAGCAA GTCATCAGAG 
     1441 ATAATCTCTA TTTGAGAACC ATCCCATGCA CTACAAGGGC TCCCAGGGAT GGGGAAGTCC 
     1501 CAGGGGTGGA TTATAACTTC ATTTCTGTTG AACAATTCAA GGCACTGGAA GAAAGCGGAG 
     1561 CATTGTTAGA AAGTGGAACG TATGATGGAA ATTTCTATGG CACACCCAAG CCTCCAGCAG 
     1621 AGCCTAGTCC TTTCCAGCCC GATCCAGTTG ATCAGGTCCT CTTTGACAAT GAGTTTGACA 
     1681 CAGAATCCCA AAGAAAAAGA ACCACATCTG TCAGCAAGAT GGAAAGGATG GACAGCTCTC 
     1741 TCCCTGAAGA AGAAGAAGAT GAGGACAAAG AAGCTGTTAA TGGCAGCGGA AGCATGGAAA 
     1801 CTAGAGAGAT GCATTCTGAG ACATCTGACT GCTGGATGAA GACTGTTCCA AGTTATAACC 
     1861 AAACAAATAG CTCCATGGAC TTTAGAAATT ATATGATGAG AGATGAGAAT CTGGAACCGC 
     1921 TGCCCAAAAA CTGGGAAATG GCCTACACTG ACACAGGAAT GATCTACTTC ATTGATCATA 
     1981 ATACGAAGAC AACCACCTGG TTGGATCCTC GTCTCTGCAA GAAAGCCAAA GCCCCTGAAG 
     2041 ACTGTGAAGA TGGAGAGCTT CCCTATGGCT GGGAGAAAAT AGAGGACCCT CAGTATGGAA 
     2101 CATACTACGT TGATCACCTC AACCAGAAAA CTCAGTTTGA AAATCCAGTG GAGGAAGCCA 
     2161 AGAGGAAAAA GCAGTTAGGA CAGGCTGAAA TTCATTCTGC AAAAACAGAT GTGGAAAGAG 
     2221 CACACTTTAC TCGGGACCCA TCCCAACTTA AAGGTGTACT TGTTCGAGCA TCGCTGAAAA 
     2281 AAAGCACCAT GGGCTTTGGC TTTACCATTA TTGGTGGAGA TAGGCCTGAT GAATTTCTAC 
     2341 AAGTAAAAAA CGTGCTCAAA GATGGTCCTG CCGCTCAGGA TGGGAAAATT GCACCAGGTG 
     2401 ATGTTATTGT AGACATCAAT GGCAACTGTG TCCTTGGTCA CACCCATGCA GATGTTGTCC 
     2461 AGATGTTTCA ACTGGTACCT GTCAACCAGT ATGTCAACCT TACTTTATGC CGTGGTTATC 
     2521 CACTTCCTGA TGACAGTGAA GATCCTGTTG TGGACATTGT TGCTGCTACC CCTGTCATCA 
     2581 ATGGACAATC TTTAACAAAG GGAGAGACAT GCATGAATAC TCAGGATTTT AAACTGGGAG 
     2641 CAATGGTTTT GGATCAGAAT GGAAAATCAG GACAAATCTT GGCCAGTGAT CGTCTCAATG 
     2701 GTCCATCTGA GTCAAGTGAG CAGAGGGCAT CTCTGGCATC ATCAGGCAGC TCACAGCCTG 
     2761 AACTAGTGAC TATCCCTCTG ATTAAGGGCC CCAAAGGCTT TGGGTTTGCA ATTGCTGACA 
     2821 GCCCAACTGG ACAGAAGGTG AAAATGATAT TGGATAGCCA GTGGTGTCAA GGCCTTCAAA 
     2881 AAGGGGATAT CATCAAAGAA ATTTACCATC AGAATGTGCA GAATTTAACA CATCTCCAAG 
     2941 TCGTAGAAGT CCTAAAGCAG TTCCCAGTAG GTGCAGATGT TCCATTGCTT ATCTTAAGAG 
     3001 GAGGTCCTTG TTCACCAACT AAAACTGCCA AAACGAAAAC AGATACAAAG GAAAATTCAG 
     3061 GAAGTTTGGA GACTATAAAT GAGCCTATTC CCCAGCCTAT GCCTTTTCCG CCCAGCATAA 
     3121 TCAGATCAGG ATCCCCAAAA TTGGATCCTT CTGAGGTCTA CCTGAAATCT AAGACTTTAT 
     3181 ATGAAGATAA ACCACCAAAC ACCAAAGATC TGGATGTCTT TCTTCGGAAA CAAGAATCAG 
     3241 GGTTTGGCTT CAGGGTGCTA GGAGGAGATG GACCTGACCA GTCTATATAT ATTGGGGCCA 
     3301 TCATTCCCCT GGGAGCAGCT GAAAAAGATG GTAGGCTCCG TGCTGCTGAT GAATTGATGT 
     3361 GTATTGATGG GATTCCTGTT AAAGGAAAAT CACACAAGCA AGTATTAGAC TTAATGACTA 
     3421 CTGCTGCTCG AAATGGACAC GTGCTACTAA CTGTCAGGAG GAAGATCTTC TATGGAGAGA 
     3481 AACAACCCGA GGACGAAAGC CATCAAGCCT TCTCACAGAA CGGATCTCCT CGCCTGAATC 
     3541 GGGCAGAGCT TCCAACCAGG TCTGCCCCAC AGGAGGCCTA TGATGTCACC TTACAGAGGA 
     3601 AAGAAAATGA AGGGTTTGGT TTTGTCATCC TCACCTCTAA AAGCAAGCCG CCCCCAGGAG 
     3661 TTATTCCTCA TAAAATTGGC CGGGTCATAG ACGGAAGTCC AGCTGATCGT TGTGGAGGAC 
     3721 TGAAAGTTGG AGATCACATC TCTGCTGTGA ATGGGCAGTC CATTGTCGAC CTATCCCATG 
     3781 ATAATATTGT TCAGCTCATC AAAGATGCTG GAGTCACCGT CACGCTGACA GTGGTTGCTG 
     3841 AAGAAGAGCA TCATGGTCCA CCATCAGGAA CAAACTCAGC CAGGCAGAGC CCAGCACTAC 
     3901 AGCACAGGCC CATGGGACAA GCACAAGCCA ACCACATACC TGGGGACAGA ATTGCCCTAG 
     3961 AAGGTGAAAT TGGGAGAGAT GTCTGCAGTT CTTACAGACA TTCCTGGTCT GACCATAAGC 
     4021 ACCTTGCACA GCCTGACACT GCAGTGATTT CAGTTGTGGG CAGTCGGCAC AATCAGAGCC 
     4081 TTGGCTGTTA CCCTGTGGAG CTGGAGAGAG GCCCTCGAGG CTTTGGGTTC AGCCTCCGCG 
     4141 GAGGGAAGGA GTACAACATG GGGCTGTTCA TCCTGCGCCT GGCCGAGGAC GGGCCCGCCA 
     4201 TCAAAGACGG CAGGATTCAC GTTGGTGACC AGATCGTTGA AATCAATGGG GAACCGACAC 
     4261 AAGGCATCAC ACACACTCGA GCAATTGAGC TCATTCAGGC TGGTGGGAAT AAAGTCCTCC 
     4321 TCCTTCTGAG GCCAGGAACT GGCTTGATAC CTGACCACGG TTTGGCTCCT TCCGGTCTGT 
     4381 GCTCCTACGT GAAACCTGAG CAACATACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4441 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6061 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6121 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6181 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6241 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6301 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6361 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6421 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6481 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6541 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6601 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6661 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6721 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6781 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6841 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6901 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6961 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7021 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7081 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7141 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7201 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7261 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7321 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7381 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7441 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7501 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7681 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7801 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7861 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7921 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7981 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8041 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8101 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8161 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8221 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//