LOCUS       MR226529                8637 bp    DNA     circular  SYN 
DEFINITION  Apaf1 (Myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1
ACCESSION   NM_001042558
VERSION     NM_001042558.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4792..4883 
                     /label="Myc-DDK"  
     misc_feature    4913..5498 
                     /label="PolyA signal"  
     misc_feature    4970..4989 
                     /label="Restriction Enzyme"  
     misc_feature    5774..6446 
                     /label="ColE1"  
     misc_feature    6876..7670 
                     /label="Kan/Neo"  
     misc_feature    7705..8007 
                     /label="SV40 ori"  
     misc_feature    8093..8570 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4774 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="Apaf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGCAAAG GCCCGCAATT GTTTGCTTCA ACATAGAGAA GCTTTGGAAA 
     1081 AGGACATCAA AACATCCTAC ATCATGGATC ACATGATCAG TAATGGCGTC TTGTCAGTGA 
     1141 TAGAGGAGGA GAAGGTCAAA AGTCAGGCCA CTCAATATCA ACGAGCAGCC GCTTTAATTA 
     1201 AAATGATACT TAATAAAGAC AACTGTGCCT ACATTTCATT CTACAACGCT CTGCTACACG 
     1261 AGGGCTATAA GGACCTTGCT GCGCTTCTGC AGAGTGGCCT TCCTCTTGTG TCGTCTTCCA 
     1321 GTGGCAAGGA CACAGATGGT GGAATAACTT CATTTGTAAG GACAGTGCTG TGTGAAGGTG 
     1381 GAGTACCCCA GAGGCCGGTT ATTTTCGTTA CTAGAAAGAA GCTGGTTCAT GCGATTCAGC 
     1441 AGAAGCTCTG GAAACTGAAT GGAGAACCAG GGTGGGTCAC CATCTATGGG ATGGCAGGCT 
     1501 GCGGCAAGTC TGTGTTAGCT GCGGAAGCCG TTCGAGATCA CTCCCTCTTA GAAGGTTGCT 
     1561 TTTCAGGGGG TGTACACTGG GTTTCCATTG GAAAACAAGA CAAATCTGGG CTTCTCATGA 
     1621 AACTGCAGAA TCTGTGCATG CGCTTGGACC AAGAAGAGAG TTTCTCTCAG AGGCTTCCAC 
     1681 TTAATATTGA GGAGGCCAAA GACCGCCTCC GTGTTCTGAT GCTGCGCAAA CACCCAAGGT 
     1741 CTCTGTTGAT CTTGGATGAT GTTTGGGATC CTTGGGTGTT AAAAGCTTTT GACAATCAGT 
     1801 GTCAGATTCT TCTTACAACC AGAGATAAGA GTGTTACAGA TTCAGTAATG GGTCCTAAGC 
     1861 ATGTTGTCCC TGTGGAGAGT GGTCTAGGGA GAGAGAAAGG ACTTGAGATC TTGTCACTTT 
     1921 TTGTTAACAT GAAGAAAGAA GATCTGCCAG CGGAGGCTCA CAGTATTATA AAGGAATGCA 
     1981 AAGGTTCTCC TCTTGTAGTG TCTTTAATTG GTGCGCTTTT ACGTGATTTT CCCAATCGCT 
     2041 GGGCATACTA CCTCAGACAG CTTCAGAATA AGCAGTTTAA GAGAATAAGG AAGTCTTCAT 
     2101 CTTATGATTA TGAGGCTCTA GATGAAGCCA TGTCGATAAG TGTTGAAATG CTCAGAGAAG 
     2161 ACATCAAAGA CTATTACACA GACCTTTCCA TCCTTCAGAA GGACGTCAAG GTACCTACAA 
     2221 AGGTGTTGTG CGTTCTCTGG GACTTGGAAA CGGAAGAAGT TGAAGACATC CTGCAGGAGT 
     2281 TCGTTAATAA GTCTCTCTTA TTCTGTAATC GGAATGGAAA GTCATTTTGT TATTATTTAC 
     2341 ATGATCTTCA AGTAGATTTT CTTACAGAGA AGAATCGCAG TCAGCTTCAG GATCTGCACA 
     2401 GGAAGATGGT CACTCAGTTT CAGAGGTATT ACCAGCCCCA CACGCTGTCT CCAGACCAGG 
     2461 AGGACTGCAT GTATTGGTAC AACTTCCTAG CCTATCACAT GGCTAGTGCC AATATGCACA 
     2521 AAGAACTTTG TGCTTTAATG TTTTCCCTGG ACTGGATTAA AGCAAAAACA GAACTTGTCG 
     2581 GCCCTGCCCA TCTGATTCAC GAGTTCGTGG CATATAGGCA TATATTGGAT GAAAAGGATT 
     2641 GTGCAGTCTG TGAGAATTTT CAAGAGTTTT TATCTTTAAA TGGACACCTC CTTGGACGAC 
     2701 AGCCATTTCC TAATATTGTA CAGCTGGGCC TCTGTGAACC AGAAACTTCC GAAGTTTATC 
     2761 GACAAGCAAA GCTGCAGGCC AAGCAGGAGG GGGATACTGG GCGCCTTTAC CTGGAATGGA 
     2821 TAAACAAAAA AACTATCAAG AATCTGTCCC GCTTAGTCGT CCGCCCCCAC ACAGATGCTG 
     2881 TTTACCACGC GTGTTTTTCT CAGGATGGTC AGAGAATAGC TTCTTGTGGG GCTGATAAAA 
     2941 CCTTACAGGT GTTCAAAGCC GAGACAGGAG AGAAACTTCT TGACATCAAA GCTCACGAAG 
     3001 ATGAGGTGCT TTGCTGCGCG TTCTCCTCAG ACGACAGTTA CATAGCGACC TGCTCAGCGG 
     3061 ATAAGAAGGT TAAGATTTGG GATTCTGCGA CTGGGAAGCT TGTGCACACC TACGACGAGC 
     3121 ACTCGGAGCA AGTCAATTGC TGCCATTTCA CCAACAAGAG TAACCACCTT CTCTTGGCCA 
     3181 CTGGGTCAAA TGATTTCTTC CTCAAGCTCT GGGATTTGAA TCAAAAAGAA TGTCGAAATA 
     3241 CCATGTTTGG TCACACGAAC TCAGTCAACC ACTGCAGGTT CTCACCAGAC GATGAGCTCT 
     3301 TGGCTAGCTG CTCAGCTGAC GGGACTTTAA GGCTTTGGGA TGTGAGATCA GCAAACGAGA 
     3361 GGAAAAGCAT TAATGTGAAG CGCTTCTTCC TGAGTTCAGA AGACCCTCCA GAGGATGTGG 
     3421 AGGTGATCGT GAAGTGTTGT TCCTGGTCTG CAGATGGTGA CAAAATAATA GTGGCAGCAA 
     3481 AAAACAAAGT CCTCCTTTTT GATATTCATA CTAGTGGCCT ATTGGCAGAG ATCCACACAG 
     3541 GCCATCACAG CACCATCCAG TACTGTGACT TCTCCCCCTA TGACCATTTG GCTGTGATTG 
     3601 CCCTGTCTCA GTACTGTGTG GAGTTGTGGA ACATAGACTC CCGCCTAAAG GTGGCCGACT 
     3661 GCAGAGGACA TTTGAGTTGG GTTCACGGTG TGATGTTTTC TCCCGATGGC TCCTCATTTT 
     3721 TGACAGCTTC TGATGACCAA ACAATAAGGG TCTGGGAGAC AAAAAAGGTA TGCAAGAACT 
     3781 CTGCCATCGT GCTAAAGCAG GAAATAGACG TCGTGTTTCA AGAGAACGAA ACGATGGTCC 
     3841 TTGCAGTTGA CAACATAAGA GGCCTGCAAC TCATTGCTGG AAAAACAGGC CAGATTGATT 
     3901 ACCTGCCTGA AGCCCAAGTG AGTTGCTGCT GCCTCAGTCC ACACCTTGAG TACGTGGCAT 
     3961 TCGGAGATGA AGATGGAGCC ATTAAGATTA TAGAACTTCC AAACAACAGA GTCTTCAGTT 
     4021 CTGGGGTTGG GCACAAGAAA GCTGTGCGGC ACATCCAGTT CACAGCTGAT GGGAAGACAC 
     4081 TGATTTCAAG TTCTGAAGAT TCTGTGATTC AGGTATGGAA TTGGCAGACA GGGGACTATG 
     4141 TATTTTTGCA AGCCCACCAG GAAACGGTAA AGGACTTCAG GCTCCTCCAA GATTCAAGAT 
     4201 TGCTTTCTTG GTCATTTGAT GGAACGGTGA AGGTGTGGAA TGTCATTACC GGAAGAATAG 
     4261 AAAGAGACTT TACTTGTCAT CAGGGCACAG TGCTTTCCTG TGCTATCTCT TCTGATGCGA 
     4321 CCAAGTTTTC ATCTACCTCT GCTGATAAGA CTGCCAAGAT CTGGAGTTTT GACCTCCTTT 
     4381 CCCCTCTTCA TGAGCTGAAG GGCCATAATG GCTGTGTCCG CTGCTCTGCC TTCTCGCTGG 
     4441 ATGGCATCCT GTTGGCAACT GGAGATGACA ATGGAGAAAT CCGGATATGG AATGTCTCAG 
     4501 ATGGCCAGCT TCTTCATTCG TGTGCTCCGA TCTCGGTAGA GGAAGGAACT GCTACCCACG 
     4561 GCGGCTGGGT AACTGATGTG TGCTTCTCTC CCGACAGTAA AACGCTTGTC TCTGCTGGAG 
     4621 GATATCTCAA GTGGTGGAAT GTTGCCACTG GGGACTCCTC ACAGACCTTC TACACAAATG 
     4681 GAACAAACCT CAAGAAAATC CACGTGTCCC CTGACTTCAG AACCTATGTG ACTGTCGATA 
     4741 ATCTCGGTAT TTTATATATT TTACAGGTTT TAGAGAGCGG ACCGACGCGT ACGCGGCCGC 
     4801 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4861 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4921 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4981 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5041 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5101 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5161 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5221 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5281 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5341 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5401 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5461 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5521 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5581 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5641 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5701 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5761 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5821 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5881 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5941 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6001 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6061 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6121 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6181 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6241 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6301 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6361 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6421 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6481 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6541 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6601 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6661 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6721 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6781 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6841 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6901 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6961 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7021 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7081 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7141 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7201 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7261 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7321 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7381 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7441 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7501 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7561 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7621 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7681 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7741 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7801 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7861 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7921 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8041 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8101 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8161 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8221 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8281 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8341 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8401 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8461 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8521 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8581 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//