LOCUS       MR226448                8067 bp    DNA     circular  SYN 
DEFINITION  Dna2 (Myc-DDK-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2)
ACCESSION   NM_177372
VERSION     NM_177372.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4224..4315 
                     /label="Myc-DDK"  
     misc_feature    4345..4930 
                     /label="PolyA signal"  
     misc_feature    4402..4421 
                     /label="Restriction Enzyme"  
     misc_feature    5206..5878 
                     /label="ColE1"  
     misc_feature    6308..7102 
                     /label="Kan/Neo"  
     misc_feature    7137..7439 
                     /label="SV40 ori"  
     misc_feature    7525..8002 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4206..4212 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4206 
                     /label="Dna2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCTG GACGAGCTGG ACCTGCTGCT GCTGGAAGAG GACGGCGGAG 
     1081 CGGAGGCCGT GCCGCGGGTG GAGCTACTTC GGAAGAAGGC GGACGCTTTG TTTCCAGAGA 
     1141 CAGTTCTGAG CCGAGGAGTG GATAACCGGT ACCTGGTGCT AGCTGTTGAG ACCTCGCAGA 
     1201 ATGAAAGAGG AGCCGAGGAG AAGCGCTTGC ACGTCACGGC GTCCCAGGAC CGAGAGCACG 
     1261 AAGTTCTGTG CATCCTGAGG AATGGCTGGA GCTCAGTACC AGTTGAGCCA GGGGACATCG 
     1321 TTCACTTAGA GGGAGACTGC ACATCTGAGC CGTGGATAAT AGACGATGAC TTTGGATATT 
     1381 TTATTCTGTA CCCGGACATG ATGATCTCCG GCACAAGCGT CGCTAGCAGC ATTCGGTGTC 
     1441 TGAGAAGAGC TGTCCTGAGC GAGACCTTCA GGGGCTCTGA TCCAGCCACT CGCCAGATGC 
     1501 TGATCGGTAC AATTCTCCAC GAAGTCTTTC AGAAAGCGAT AAGTGAGAGC TTTGCTCCAG 
     1561 AAAGGCTACA AGAGCTTGCT CTTCAGACCC TCCGAGAAGT GAGGCATTTG AAGGAAATGT 
     1621 ATCGCTTGAA TCTGAGCCAA GATGAAATAC TCTGTGAAGT TGAGGAGTAT CTCCCTTCTT 
     1681 TCTCTAAGTG GGCGGAGGAC TTCATGCGTA AGGGCCCTTC GTCTGAATTC CCTCAAATGC 
     1741 AGCTCTCTCT GCCAAGCGAT GGCAGTAACA GAAGTTCACC TTGTAACATT GAAGTAGTCA 
     1801 AGTCACTGGA TATCGAAGAA AGCATCTGGT CCCCTAGGTT TGGACTGAAG GGCAAAATAG 
     1861 ACGTCACAGT CGGGGTCAAA ATACATCGAG ATTGTAAAAT GAAATACAAG GTAATGCCAC 
     1921 TGGAGCTGAA GACCGGCAAA GAGTCCAACT CCATCGAGCA CCGGAGTCAG GTGGTCCTGT 
     1981 ACACGCTGCT AAGCCAGGAG AGGCGAGAGG ACCCCGAGGC TGGCTGGCTT CTCTACCTCA 
     2041 AGACCGGGCA GATGTACCCT GTGCCCGCCA ACCATCTGGA CAAAAGAGAA CTGCTGAAGC 
     2101 TCAGGAACTG GCTGGCCGCC TCGCTGCTGC ACCGTGTGAG CCGAGCAGCT CCGGGGGAGG 
     2161 AAGCTCGGCT CTCTGCCTTG CCACAGATAA TCGAGGAAGA GAAAACTTGT AAATACTGTT 
     2221 CGCAGATAGG CAACTGTGCT CTTTATAGCA GAGCAGTTGA AGAGCAGGGA GATGACGCAT 
     2281 CCATCCCAGA AGCCATGCTG TCCAAAATCC AGGAAGAAAC ACGGCACCTG CAGCTGGCGC 
     2341 ACTTGAAGTA TTTCAGCCTG TGGTGTCTCA TGTTAACGCT GGAGTCGCAA TCTAAAGACA 
     2401 ACCGGAAGAC TCACCAGAGT ATCTGGTTAA CGCCTGCCTC TGAACTGGAG GAGAGTGGCA 
     2461 ACTGTGTTGG AAACCTGGTC AGGACGGAGC CTGTATCAAG AGTTTGTGAT GGACAGTATT 
     2521 TACATAATTT TCAGCGGAAA AATGGTCCCA TGCCAGCCAC CAATCTGATG GCAGGTGACA 
     2581 GGATTATTTT AAGTGGAGAA GAGAGAAAAC TATTTGCTTT GTCCAAAGGC TACGTGAAGA 
     2641 AGATGAACAA GGCAGCCGTT ACCTGCTTGC TAGACAGGAA CTTGTCGACA CTCCCAGCAA 
     2701 CAACGGTGTT TCGATTAGAC CGGGAAGAAA GACATGGCGA CATAAGTACT CCACTAGGAA 
     2761 ATCTGTCTAA ATTGATGGAA AGCACAGATC CCAGCAAAAG ACTTCGAGAA TTAATCATCG 
     2821 ATTTCAGGGA ACCTCAGTTC ATCGCCTACC TCAGCTCCGT CCTTCCACAC GATGCGAAGG 
     2881 ATACGGTGGC CAACATTCTA AAGGGTTTGA ATAAACCCCA GAGACAAGCA ATGAAGAGAG 
     2941 TCCTTCTTTC GAAAGACTAC ACGCTCATTG TTGGGATGCC AGGGACAGGA AAGACAACCA 
     3001 CCATCTGTGC CCTGGTGAGG ATCCTCTCTG CCTGTGGGTT CAGCGTTCTG CTGACCAGCT 
     3061 ACACGCACTC CGCCGTTGAC AATATTCTCC TGAAGTTAGC CAAGTTTAAA GTCGGATTTT 
     3121 TGCGCTTGGG TCAGTCTCAT AAGGTCCATC CAGACATCCA GAAGTTTACA GAGGAGGAGA 
     3181 TTTGCAGATC GAGGTCCATT GCGTCTTTAG CCCATCTAGA AGAACTCTAT AACAGTCACC 
     3241 CTATTGTTGC AACAACCTGC ATGGGAATAA ATCACCCAAT ATTTTCCCGA AAAACCTTTG 
     3301 ATTTCTGTAT TGTGGATGAA GCATCTCAAA TCAGCCAGCC CGTCTGCCTG GGCCCACTGT 
     3361 TCTTTTCGCG GAGGTTTGTG CTGGTGGGGG ACCATCAGCA GCTGCCTCCC CTGGTTGTAA 
     3421 ACCGAGAGGC AAGAGCTCTG GGCATGAGCG AGAGCTTGTT CAAGAGGCTG GAGCGGAACG 
     3481 AGAGCGCTGT GGTGCAGCTG ACCGTGCAAT ACCGAATGAA CAGGAAGATT ATGTCCCTGA 
     3541 GCAACAAGCT CACATACGCA GGGAAACTGG AGTGTGGGTC AGACCGAGTG GCCAACGCAG 
     3601 TGCTAGCCCT GCCCAACCTC AAGGACGCAA GGCTGAGCCT CCAGCTTTAT GCCGACTACT 
     3661 CTGACAGCCC CTGGCTGGCT GGAGTGTTAG AGCCAGACAA TCCCGTGTGT TTCCTTAACA 
     3721 CAGACAAGGT CCCAGCTCCA GAGCAAGTTG AAAATGGTGG TGTTAGCAAT GTCACAGAAG 
     3781 CCAGACTCAT TGTCTTCCTA ACCTCAACTT TTATAAAGGC AGGCTGCAGC CCCTCAGACA 
     3841 TTGGCGTCAT CGCCCCGTAC CGACAGCAGC TGCGGATCAT CAGCGACTTA CTGGCCCGGT 
     3901 CTTCTGTTGG GATGGTTGAG GTTAACACAG TAGACAAATA CCAAGGAAGG GACAAGAGTC 
     3961 TCATCCTGGT GTCCTTTGTT AGGAGTAATG AGGATGGAAC TCTCGGCGAG CTCCTGAAAG 
     4021 ATTGGCGGAG ACTCAACGTG GCACTCACCA GAGCCAAGCA TAAACTGATC CTGCTGGGCA 
     4081 GCGTGTCCTC GCTGAAGCGC TTCCCGCCTC TGGGGACGCT CTTTGACCAT CTGAATGCAG 
     4141 AGCAGTTAAT CCTGGACCTT CCTTCCAGAG AGCATGAGAG CCTCAGCCAC ATCCTGGGCG 
     4201 ATTGTCAGCG AGACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4261 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4321 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4381 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4441 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4501 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4561 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4621 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4681 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4741 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4801 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4861 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4921 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4981 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5041 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5101 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5161 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5221 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5281 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5341 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5401 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5461 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5521 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5581 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5641 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5701 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5761 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5821 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5881 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5941 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6001 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6061 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6121 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6181 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6241 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6301 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6361 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6421 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6481 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6541 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6601 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6661 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6721 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6781 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6841 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6901 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6961 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7021 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7081 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7141 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7201 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7261 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7321 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7381 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7441 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7501 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7561 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7621 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7681 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7741 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7801 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7861 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7921 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7981 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8041 ATTTAACAAA AATTTAACGC GAATTTT
//