LOCUS       MR226368                8649 bp    DNA     circular  SYN 
DEFINITION  Nphs1 (Myc-DDK-tagged) - Mouse nephrosis 1 homolog, nephrin (human) (Nphs1)
ACCESSION   NM_019459
VERSION     NM_019459.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="Nphs1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGCTAAG GAAGCCACAG TCAGAGGCCC CGGGGCAAGC CCAGTGCACA 
     1081 GAACTTGCCA CCTGATTCCC CTGCTGCTCG CGGGAATGCT GACCACAGGC CTGGCCCAGT 
     1141 CGCCAGTCCC CACCTCAGCA CCTCGAGGCT TCTGGGCTCT ATCTGAAAAC CTGACTGTGG 
     1201 TGGAAGGGTC GACAGTTAAG CTGTGGTGTG GCGTCAGGGC CCCCGGCAGT GTGGTGCAGT 
     1261 GGGCTAAGGA TGGGCTGCTT CTGGGTCCAA ACCCTAAGAT TCCAGGCTTC CCAAGGTACA 
     1321 GCCTGGAAGG AGACAGTGCT AAAGGTGAGT TCCACCTGCT TATTGAAGCC TGTGACCTCA 
     1381 GCGATGATGC GGAGTACGAG TGCCAAGTCG GCCGCTCCGA GTTGGGTCCC GAGCTCGTGT 
     1441 CTCCCAGCGT AATCCTCTCC ATCCTAGTTT CCCCCAAGGT GCTTCAGTTA ACCCCTGAGG 
     1501 CAGGAAGCAC AGTTACCTGG GTAGCTGGAC AGGAATATGT GGTCACCTGT GTGTCTGGGG 
     1561 ATGCAAAGCC AGCACCTGAC ATCATCTTCA TCCAGGGTGG ACGCACGGTA GAGGATGTCT 
     1621 CCTCCAGCGT GAATGAGGGA TCAGAGGAGA AACTCTTCTT CACGGAAGCC GAAGCCAGGG 
     1681 TGACACCCCA GAGCTCAGAT AATGGGCAGC TGCTAGTCTG CGAGGGGTCC AACCCAGCCT 
     1741 TGGCCACTCC CATAAAGGCT TCGTTCACCA TGAATATCCT GTTCCCCCCA GGACCTCCTG 
     1801 TCATTGATTG GCCAGGCCTG AATGAGGGGC ATGTACGGGC AGGGGAGAAC CTGGAGCTAC 
     1861 CCTGCATAGC CAGAGGTGGA AATCCACCTG CGACCCTGCA GTGGCTGAAG AATGGTAAAC 
     1921 CAGTGTCCAT AGCTTGGGGC ACAGAGCATG CCCAGGCAGT GGCTCACAGT GTGCTGGTGA 
     1981 TGACCGTTCG ACCTGAAGAC CACGGAGCTC GGCTCAGCTG TCAGTCCTAC AACAGTGTGT 
     2041 CTGCAGAGAC CCAGGAGCGA AGCATCACTC TGCAGGTCAC CTTTCCCCCC AGTGCCGTTA 
     2101 CCATCCTGGG ATCTACATCA CAGTCTGAGA ACAAAAATGT GACCCTTTGC TGCCTTACCA 
     2161 AGTCCAGTCG CCCACGGGTC CTGCTGCGAT GGTGGTTGGG TGGACGGCAG TTGCTGCCCA 
     2221 CGGATGAGAC AGTCATGGAT GGCCTGCATG GTGGCCACAT CTCCATGTCC AATCTGACAC 
     2281 TCTTGGTGAA GAGAGAAGAC AATGGCCTGT CCCTCACCTG CGAAGCCTTC AGTGATGCCT 
     2341 TCAGCAAGGA GACCTTCAAG AAGTCACTCA CCTTGAATGT AAAATACCCG GCCCAGAAGC 
     2401 TGTGGATTGA GGGACCCCCA GAGGGGCAGA GCATCCGGAC TGGAACTCGG GTGAGGCTGG 
     2461 TATGCTTGGC CATTGGAGGC AACCCAGAGC CCTCCCTCAC CTGGCTTAAG GATTCGCGCC 
     2521 CGGTGAACGA TCCTCGGCAG TCTCAGGAGC CCCGGCGTGT GCAGCTGGGC AGTGTGGAGA 
     2581 AGTCCGGCAG CACTTTCTCC CGCGAGCTGG TGCTGATCAT AGGCCCGCCG GACAACCTAG 
     2641 CCAAGTTCTC CTGCAAGGCG GGTCAGCTCA GTGCGTCTAC GCAGCTGGTG GTGCAGTTCC 
     2701 CCCCAACAAA CCTGACCATC CTGGCCAACT CGTCCGCACT GCGCCCAGGC GACGCCTTGA 
     2761 ACTTGACCTG CGTCAGCATC AGCAGCAACC CTCCAGTTAA CTTGTCTTTG GACAAGGAAG 
     2821 GAGAGAGGCT GGACGACGTG GCTGCAAAAC CCCAGAGCGC CCCATTCAAA GGCTCCGCTG 
     2881 CATCCAGGAG TGTTTTTCTT CGGGTGTCAT CCCGAGACCA CGGTCACCGG GTTACCTGCC 
     2941 GGGCCCACAG CGAGGCACTT CGTGAAACCG TGAGCTCTTT CTACCGCCTC AACGTGTTAT 
     3001 ACCCTCCAGA GTTCCTGGGA GAGCAAGTGC GGGCAGTGAC CGTGGTGGAG CAGGGCCAGG 
     3061 CACTGCTGCC TGTGTCCGTG TCTGCTAACC CCGCCCCCGA GGCCTTCAAC TGGACCTTCC 
     3121 GAGGCTACCG CCTCAGCCCA GCTGGGGGTC CCCGGCACCG CATCCTGTCT GGAGGGGCAC 
     3181 TGCAGCTGTG GAATGTAACC CGAGCTGACG ATGGCTTTTA TCAGCTACAC TGCCAGAACT 
     3241 CGGAGGGCAC CGCCGAGGCG CTGTTGAAGC TGGACGTGCA TTATGCTCCC ACCATCCGTG 
     3301 CCCTGAAGGA CCCTACTGAG GTGAATGTTG GGGGTTCTGT GGATATAGTC TGCACCGTCG 
     3361 ATGCCAATCC CATCCTCCCA GAGATGTTCA GCTGGGAGAG ACTGGGGGAA GACGAGGAGG 
     3421 AACTGAACCT GGACGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT CTGCGGATTC 
     3481 GCCAGGCCAA GCTGTCCCAG GCTGGCGCTT ACCAGTGCAT CGTGGACAAC GGGGTGGCTC 
     3541 CCGCAGCCAG AGGACTGGTT CGTCTTGTTG TCCGATTTGC CCCCCAGGTG GATCATCCTA 
     3601 CTCCCCTGAC GAAAGTGGCT GCTGCTGGGG ACAGCACCAG CTCAGCCACA CTCCACTGCC 
     3661 GTGCCCGGGG GGTCCCCAAC ATCGACTTCA CTTGGACCAA AAATGGGGTC CCTCTGGATC 
     3721 TCCAAGACCC CAGGTACACA GAGCACAAGT ACCACCAGGG TGTGGTCCAC AGCAGCCTCC 
     3781 TGACCATTGC TAATGTGTCT GCAGCCCAGG ACTATGCCCT CTTCAAATGC ACAGCCACCA 
     3841 ATGCCCTTGG TTCAGACCAC ACCAACATCC AGCTCGTCAG CATCAGCCGT CCTGACCCTC 
     3901 CACTGGGGCT GAAGGTTGTG AGCGTGAGCC CTCACTCGGT AGGACTGGAG TGGAAACCTG 
     3961 GCTTTGATGG GGGTCTGCCT CAGAGGTTCC AAATCAGGTA TGAGGCCCTC GAGAGCCCAG 
     4021 GATTCCTCTA CATGGATGTC CTGCCTGCAC AGGCCACCAC CTTCACACTG ACTGGGCTGA 
     4081 AGCCTTCTAC ACGGTACAGG ATCTGGCTGT TGGCCAGCAA TGCCCTGGGG GACAGTGGAT 
     4141 TGACGGACAA GGGGATCCAG GTCTCCATCA CTACCCCAGG CCTGGACCAG GCTCCTGAAG 
     4201 ACACAGACCA GCCGCTGCCC ACAGAGCAGC CTCCGGGACC CCCGAGGCTG CCCCTGCTGC 
     4261 CTGTGCTCTT TGCGGTTGGT GGTCTTCTGT TGCTTTCCAA TGCCTCCTGT GTTGGGGGAC 
     4321 TCCTCTGGCG GAGAAGACTG AGGCGCCTTG CTGAGGAGAT CTCAGAGAAG ACAGAGGCAG 
     4381 GGTCGGAGGA GGATCGAATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT GGGGACCGGG 
     4441 ACACAAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC ACACTACTAC TCCATGAGGG 
     4501 ACTTCAGTCC CCAGCTTCCC CCAACACTGG AAGAGGTGTC ATATCGCCAA GCCTTCACAG 
     4561 GTATTGAAGA TGAGGACATG GCCTTCCCCG GACACCTGTA TGACGAGGTG GAGAGAGTCT 
     4621 ATGGCCCACC TGGGGTCTGG GGACCCCTCT ATGATGAAGT ACAAATGGAC CCCTATGACC 
     4681 TTCGCTGGCC TGAGGTCAAA TATGAGGACC CAAGGGGAAT CTACGACCAG GTGGCGGCAG 
     4741 ACATGGATGC TGGGGAGCCT GGTTCTCTGC CCTTTGAGCT GAGGGGACAT CTGGTGACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//