LOCUS       MR225685                6816 bp    DNA     circular  SYN 
DEFINITION  Chat (Myc-DDK-tagged) - Mouse choline acetyltransferase (Chat)
ACCESSION   NM_009891
VERSION     NM_009891.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2973..3064 
                     /label="Myc-DDK"  
     misc_feature    3094..3679 
                     /label="PolyA signal"  
     misc_feature    3151..3170 
                     /label="Restriction Enzyme"  
     misc_feature    3955..4627 
                     /label="ColE1"  
     misc_feature    5057..5851 
                     /label="Kan/Neo"  
     misc_feature    5886..6188 
                     /label="SV40 ori"  
     misc_feature    6274..6751 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2955..2961 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2955 
                     /label="Chat"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTATCCTG GAAAAGGTCC CCCCAAAGAT GCCTGTACAA GCTTCTAGCT 
     1081 GTGAGGAGGT GCTGGACTTA CCTAAGTTGC CAGTGCCCCC ACTGCAGCAA ACCCTGGCCA 
     1141 CCTACCTTCA GTGCATGCAA CACCTGGTAC CTGAAGAGCA GTTCAGGAAG AGCCAGGCCA 
     1201 TTGTGAAGCG GTTTGGGGCC CCTGGTGGCC TGGGTGAGAC CCTGCAGGAA AAGCTCTTGG 
     1261 AGAGACAGGA GAAGACAGCC AATTGGGTCT CTGAATACTG GCTGAATGAC ATGTATCTAA 
     1321 ACAACCGCCT GGCCCTGCCA GTCAACTCTA GCCCTGCTGT GATCTTTGCT CGGCAGCACT 
     1381 TCCAAGACAC CAATGACCAG CTAAGGTTTG CAGCCAGCCT CATCTCTGGT GTGCTTAGCT 
     1441 ACAAGGCTCT GCTGGACAGC CAATCCATTC CCACTGACTG GGCCAAGGGG CAGCTCTCAG 
     1501 GGCAGCCTCT CTGTATGAAG CAGTACTACA GACTCTTCTC ATCATACCGG CTTCCTGGCC 
     1561 ATACCCAGGA CACACTGGTG GCCCAGAAGA GCAGTATCAT GCCTGAGCCC GAGCATGTCA 
     1621 TCGTGGCCTG CTGCAACCAG TTCTTTGTCT TGGATGTTGT CATTAATTTC CGCCGTCTCA 
     1681 GTGAGGGTGA TCTGTTCACT CAGTTGAGAA AGATAGTCAA AATGGCGTCC AACGAGGATG 
     1741 AACGCCTGCC TCCAATCGGC CTGCTGACGT CAGACGGGAG GAGCGAGTGG GCCAAGGCCA 
     1801 GGACGGTCCT CTTAAAAGAC TCCACCAACC GGGACTCCCT GGACATGATC GAGCGCTGCA 
     1861 TCTGCCTGGT ATGCCTGGAT GGTCCAGGCA CTGGAGACCT CAGTGACACA CACAGGGCCC 
     1921 TCCAGCTCCT TCATGGTGGA GGCTGCAGCT TGAATGGAGC GAATCGTTGG TATGACAAGT 
     1981 CCCTGCAGTT TGTGGTGGGC CGAGATGGCA CCTGCGGTGT GGTGTGTGAG CACTCCCCTT 
     2041 TTGATGGCAT CGTCCTGGTG CAGTGCACGG AGCACCTGCT GAAACATATG ATGACAGGCA 
     2101 ACAAGAAGCT CGTCCGAGCT GACTCAGTGA GTGAACTCCC TGCTCCCAGA AGGCTGAGGT 
     2161 GGAAATGTTC CCCAGAAACT CAAGGCCATC TCGCCTCCTC GGCAGAGAAA CTTCAAAGAA 
     2221 TCGTAAAGAA TCTGGATTTC ATTGTTTATA AGTTTGACAA CTATGGGAAA ACATTTATTA 
     2281 AGAAGCAGAA ATGCAGTCCT GATGGCTTCA TCCAGGTGGC CCTCCAGCTG GCTTACTACA 
     2341 GGCTTTACCA GAGGCTGGTG CCCACCTATG AGAGTGCATC CATCCGCCGC TTCCAGGAAG 
     2401 GTCGGGTGGA CAACATCAGA TCGGCCACTC CTGAGGCTCT GGCTTTTGTG CAAGCCATGA 
     2461 CTGACCACAA GGCTGCCGTG CTGGCTTCTG AGAAACTGCA GCTGCTGCAG AGGGCCATCC 
     2521 AGGCCCAAAC TGAGTACACA GTCATGGCCA TAACCGGCAT GGCCATTGAC AACCATCTTC 
     2581 TGGCACTGAG GGAGCTGGCC CGAGACCTGT GCAAAGAGCC ACCTGAGATG TTCATGGATG 
     2641 AAACATACCT GATGAGCAAC CGGTTTATTC TCTCCACCAG CCAGGTGCCC ACGACCATGG 
     2701 AGATGTTCTG CTGTTATGGC CCTGTGGTAC CCAATGGGTA TGGAGCCTGT TATAACCCCC 
     2761 AGCCTGAGGC CATCACCTTC TGCATCTCCA GCTTTCACGG CTGCAAAGAG ACCTCATCTG 
     2821 TGGAGTTTGC AGAAGCGGTG GGAGCGAGCC TTGTTGACAT GAGAGACCTC TGTAGTTCGA 
     2881 GGCAGCCTGC TGAAGGCAAG CCACCAACAG CAAAGGAAAG AGCTAGAGGC CCAACCAAGC 
     2941 CAAGCAATCT TGACTACTCC CACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3001 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3061 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3121 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3181 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3241 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3301 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3361 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3421 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3481 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3541 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3601 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3661 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3721 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3781 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3841 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3901 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3961 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4021 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4081 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4141 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4201 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4261 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4321 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4381 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4441 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4501 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4561 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4621 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4681 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4741 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4801 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4861 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4921 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4981 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5041 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5101 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5161 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5221 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5281 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5341 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5401 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5461 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5521 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5581 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5641 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5701 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5761 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5821 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5881 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5941 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6001 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6061 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6121 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6181 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6241 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6301 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6361 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6421 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6481 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6541 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6601 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6661 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6721 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6781 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//