LOCUS       MR225593                8616 bp    DNA     circular  SYN 
DEFINITION  Nid1 (Myc-DDK-tagged) - Mouse nidogen 1 (Nid1)
ACCESSION   NM_010917
VERSION     NM_010917.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4773..4864 
                     /label="Myc-DDK"  
     misc_feature    4894..5479 
                     /label="PolyA signal"  
     misc_feature    4951..4970 
                     /label="Restriction Enzyme"  
     misc_feature    5755..6427 
                     /label="ColE1"  
     misc_feature    6857..7651 
                     /label="Kan/Neo"  
     misc_feature    7686..7988 
                     /label="SV40 ori"  
     misc_feature    8074..8551 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4755..4761 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4755 
                     /label="Nid1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGACGCG AGCGGCTGTA GTTGGGCGAT GTGGACGTGG GCGCTGTTGC 
     1081 AGCTGCTGCT ACTAGTGGGG CCCGGAGGCT GCCTGAATCG CCAGGAGCTC TTCCCCTTCG 
     1141 GCCCTGGGCA GGGGGACCTA GAGCTGGAGG CCGGGGACGA CGTGGTATCC CCCTCCCTGG 
     1201 AACTGATCGG AGAACTGAGC TTCTATGATC GTACGGACAT CACGTCGGTC TATGTCACCA 
     1261 CAAATGGTAT CATCGCCATG AGCGAACCCC CAGCCACAGA ATACCATCCC GGAACCTTCC 
     1321 CACCCAGCTT CGGCTCAGTA GCGCCTTTCC TGGCTGACTT GGACACAACC GATGGCCTGG 
     1381 GGAATGTGTA TTATCGAGAA GACTTATCCC CGTTCATTAT TCAGATGGCA GCAGAGTATG 
     1441 TCCAGAGAGG CTTCCCGGAG GTCTCTTTCC AGCCCACTAG TGTGGTGGTT GTCACTTGGG 
     1501 AATCTGTGGC CCCTTATGGA GGGCCCAGCA GCAGCCCTGC CGAGGAAGGC AAGAGAAACA 
     1561 CGTTCCAGGC TGTTCTGGCC TCCTCAAATT CCAGCTCCTA TGCCATTTTC CTTTATCCTG 
     1621 AAGATGGTCT ACAGTTCTTT ACAACATTCT CGAAGAAGGA TGAAAGCCAA GTACCTGCTG 
     1681 TGGTTGGTTT CAGCAAAGGT CTAGTAGGAT TTCTATGGAA GAGCAACGGA GCCTATAACA 
     1741 TATTTGCCAA TGACAGAGAA TCAATTGAAA ATTTGGCTAA GAGCAGCAAC GCTGGGCACC 
     1801 AGGGTGTGTG GGTGTTTGAG ATTGGAAGTC CTGCTACCGC CAAGGGCGTG GTGTCTGCAG 
     1861 ATGTGAACCT GGACCTGGAC GACGATGGAG CAGACTATGA AGATGAAGAT TATGACCTAG 
     1921 TAACCTCTCA CCTTGGCCTG GAGGATGTAG CCACTCCCTC CCCCTCCCAC AGTCCAAGAA 
     1981 GGGGATACCC TGACCCACAC AATGTACCTA GGATCCTCTC TCCTGGCTAT GAGGCTACAG 
     2041 AGAGACCCCG TGGAGTCCCC ACTGAGAGGA CCAGATCTTT CCAGCTGCCA GCGGAGAGGT 
     2101 TCCCTCAGCA TCACCCCCAG GTCATTGATG TGGATGAAGT AGAGGAAACA GGAGTTGTAT 
     2161 TCAGCTACAA CACAGGTTCC CAGCAGACTT GTGCCAACAA TAGACACCAG TGCTCCGTGC 
     2221 ATGCAGAGTG CAGAGACTAT GCTACTGGCT TCTGCTGCAG GTGTGTGGCC AACTACACAG 
     2281 GCAATGGCAG ACAGTGCGTG GCAGAAGGCT CTCCACAACG GGTCAATGGC AAGGTGAAGG 
     2341 GAAGGATCTT CGTGGGGAGC AGCCAGGTCC CCGTGGTGTT TGAGAACACT GACCTGCACT 
     2401 CCTATGTGGT GATGAACCAC GGGCGCTCTT ACACAGCCAT CAGCACCATC CCTGAAACCG 
     2461 TCGGCTACTC TCTGCTCCCC CTGGCACCCA TTGGAGGCAT CATCGGATGG ATGTTTGCAG 
     2521 TGGAGCAGGA TGGGTTCAAG AATGGGTTTA GCATCACTGG GGGCGAGTTT ACCCGGCAAG 
     2581 CTGAGGTGAC CTTCCTGGGG CACCCAGGCA AGCTGGTCCT GAAGCAGCAG TTCAGCGGTA 
     2641 TTGATGAACA TGGACACCTG ACCATCAGCA CGGAGCTGGA GGGCCGCGTG CCGCAGATCC 
     2701 CCTATGGAGC CTCGGTGCAC ATTGAGCCCT ACACCGAACT GTACCACTAC TCCAGCTCAG 
     2761 TGATCACTTC CTCCTCCACC CGGGAGTACA CGGTGATGGA GCCTGATCAG GACGGCGCTG 
     2821 CACCCTCACA CACCCATATT TACCAGTGGC GTCAGACCAT CACCTTCCAG GAGTGTGCCC 
     2881 ACGATGACGC CAGGCCAGCC CTGCCCAGCA CCCAGCAGCT CTCTGTGGAC AGCGTGTTTG 
     2941 TCCTGTACAA CAAGGAGGAG AGGATCTTGC GCTATGCCCT CAGCAACTCC ATCGGGCCTG 
     3001 TGAGGGATGG CTCCCCTGAT GCCCTTCAGA ATCCATGCTA CATTGGCACC CATGGGTGTG 
     3061 ACAGCAATGC TGCCTGTCGC CCTGGCCCTG GAACACAGTT CACCTGCGAA TGCTCCATCG 
     3121 GCTTCCGAGG AGACGGGCAG ACTTGCTATG ATATTGATGA GTGTTCAGAG CAGCCTTCCC 
     3181 GCTGTGGGAA CCATGCGGTC TGCAACAACC TCCCAGGAAC CTTCCGCTGC GAGTGTGTAG 
     3241 AGGGCTACCA CTTCTCAGAC AGGGGAACAT GTGTGGCTGC CGAGGACCAA CGTCCCATCA 
     3301 ACTACTGTGA AACTGGTCTC CACAACTGTG ATATCCCCCA GCGAGCCCAG TGCATCTATA 
     3361 TGGGTGGTTC CTCCTACACC TGCTCCTGTC TGCCTGGCTT CTCTGGGGAT GGCAGAGCCT 
     3421 GCCGAGACGT GGATGAATGC CAGCACAGCC GATGTCACCC CGATGCCTTC TGCTACAACA 
     3481 CACCAGGCTC TTTCACATGT CAGTGCAAGC CTGGCTATCA GGGGGATGGC TTCCGATGCA 
     3541 TGCCCGGAGA GGTGAGCAAA ACCCGGTGTC AACTGGAACG AGAGCACATC CTTGGAGCAG 
     3601 CCGGCGGGGC AGATGCACAG CGGCCCACCC TGCAGGGGAT GTTTGTACCT CAGTGTGATG 
     3661 AATATGGACA CTATGTACCC ACCCAGTGTC ACCACAGCAC TGGCTACTGC TGGTGTGTGG 
     3721 ACCGAGATGG TCGGGAGCTG GAGGGTAGCC GTACCCCACC TGGGATGAGG CCCCCGTGTC 
     3781 TGAGTACAGT GGCTCCTCCT ATTCACCAGG GACCAGTAGT ACCTACAGCT GTCATCCCCC 
     3841 TGCCTCCAGG GACACACTTA CTCTTTGCTC AGACTGGAAA GATTGAACGC CTGCCCCTGG 
     3901 AGAGAAACAC CATGAAGAAG ACAGAAGCCA AGGCCTTTCT CCATATCCCT GCAAAAGTCA 
     3961 TCATTGGACT GGCCTTTGAC TGCGTGGACA AGGTGGTTTA CTGGACAGAC ATCAGCGAGC 
     4021 CTTCCATTGG GAGAGCCAGC CTCCACGGTG GAGAGCCAAC CACCATCATT CGACAAGATC 
     4081 TTGGAAGCCC TGAAGGCATT GCCCTTGACC ATCTTGGTCG AACCATCTTC TGGACGGACT 
     4141 CTCAGTTGGA TCGAATAGAA GTTGCAAAGA TGGATGGCAC CCAGCGCCGA GTGCTGTTTG 
     4201 ACACGGGTTT GGTGAATCCC AGAGGCATTG TGACAGACCC CGTAAGAGGG AACCTTTATT 
     4261 GGACAGATTG GAACAGAGAC AATCCCAAAA TTGAGACTTC TCACATGGAT GGCACCAACC 
     4321 GGAGGATTCT CGCACAGGAC AACCTGGGCT TGCCCAATGG TCTGACCTTT GATGCATTCT 
     4381 CATCTCAGCT TTGCTGGGTG GATGCAGGCA CCCATAGGGC AGAATGCCTG AACCCAGCTC 
     4441 AGCCCGGCAG ACGCAAAGTT CTCGAAGGGC TCCAGTATCC TTTCGCTGTG ACTAGCTATG 
     4501 GGAAGAATTT GTACTACACA GACTGGAAGA CGAATTCAGT GATTGCCATG GACCTTGCTA 
     4561 TATCCAAAGA GATGGATACC TTCCACCCAC ACAAGCAGAC CCGGCTATAT GGCATCACCA 
     4621 TCGCCCTGTC CCAGTGTCCC CAAGGCCACA ATTACTGCTC AGTGAATAAT GGTGGATGTA 
     4681 CCCACCTCTG CTTGCCCACT CCAGGGAGCA GGACCTGCCG ATGTCCTGAC AACACCCTGG 
     4741 GAGTTGACTG CATTGAACGG AAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4801 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4861 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4921 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4981 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5041 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5101 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5161 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5221 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5281 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5341 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5401 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5461 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5521 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5581 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5641 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5701 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5761 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5821 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5881 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5941 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6001 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6061 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6121 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6181 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6241 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6301 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6361 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6421 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6481 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6541 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6601 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6661 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6721 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6781 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6841 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6901 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6961 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7021 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7081 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7141 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7201 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7261 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7321 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7381 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7441 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7501 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7561 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7621 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7681 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7741 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7801 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7861 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7981 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8041 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8101 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8161 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8221 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8281 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8341 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8401 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8461 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8521 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8581 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//