LOCUS       MR225337                7161 bp    DNA     circular  SYN 
DEFINITION  Pkd2l1 (Myc-DDK-tagged) - Mouse polycystic kidney disease 2-like 1 (Pkd2l1)
ACCESSION   NM_181422
VERSION     NM_181422.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3318..3409 
                     /label="Myc-DDK"  
     misc_feature    3439..4024 
                     /label="PolyA signal"  
     misc_feature    3496..3515 
                     /label="Restriction Enzyme"  
     misc_feature    4300..4972 
                     /label="ColE1"  
     misc_feature    5402..6196 
                     /label="Kan/Neo"  
     misc_feature    6231..6533 
                     /label="SV40 ori"  
     misc_feature    6619..7096 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3300..3306 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3300 
                     /label="Pkd2l1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATAGTATG GAAAGCCCCA AGAATCAGGA GCTACAAACC CTGGGGAACA 
     1081 GAGCCTGGGA CAATCCTGCC TACAGCGACC CTCCTTCCCC GAACAGGACG CTGAGGATCT 
     1141 GCACTGTCTC CAGTGTGGCT CTCCCTGAGA CTCAACCCAA AAAGCCAGAA GTCAGATGCC 
     1201 AGGAGAAGAC ACAGAGAACC CTGGTGTCCA GCTGCTGTCT CCATATCTGT CGGAGCATCA 
     1261 GAGGACTGTG GGGGACAACG CTGACTGAGA ACACAGCCGA GAACAGGGAG CTTTATGTCA 
     1321 AGACCACCCT GAGGGAGCTT GTGGTATACA TAGTGTTCCT CGTGGACATC TGTCTGTTGA 
     1381 CCTACGGAAT GACAAGTTCT AGTGCCTATT ACTACACCAA AGTGATGTCT GAATTGTTCC 
     1441 TACACACCCC ATCCGACTCT GGAGTCTCCT TCCAAACCAT CAGCAGCATG TCAGACTTCT 
     1501 GGGATTTTGC TCAGGGCCCA CTCCTGGACA GTTTGTACTG GACAAAGTGG TACAACAACC 
     1561 AGAGCCTGGG GCGTGGCTCC CACTCCTTCA TCTACTATGA GAACCTGCTC CTGGGAGCCC 
     1621 CAAGGTTGCG GCAGCTGCGC GTGCGCAATG ACTCCTGTGT GGTTCATGAA GACTTCCGGG 
     1681 AGGACATTTT GAACTGTTAT GATGTGTACT CGCCGGACAA AGAAGATCAG CTCCCCTTTG 
     1741 GACCTCAGAA CGGCACAGCG TGGACATACC ATTCCCAGAA TGAGCTGGGT GGCTCCTCCC 
     1801 ACTGGGGCAG GCTCACAAGC TACAGCGGGG GTGGCTACTA CTTGGATCTT CCAGGATCCC 
     1861 GACAAGCCAG TGCAGAGGCC CTCCAAGGAC TCCAGGAGGG ACTGTGGCTG GACAGGGGCA 
     1921 CTCGGGTGGT CTTTATCGAC TTCTCCGTCT ACAATGCCAA CATCAATCTT TTCTGTATTC 
     1981 TGAGACTGGT GGTAGAGTTT CCAGCCACAG GAGGGACCAT CCCATCCTGG CAGATCCGCA 
     2041 CAGTTAAGCT GATCCGCTAT GTGAATAACT GGGACTTCTT CATTGTGGGC TGTGAAGTTG 
     2101 TCTTCTGTGT CTTCATCTTC TATTATGTGG TGGAGGAAAT CCTGGAAATC CACCTGCATC 
     2161 GGCTTCGCTA CCTCAGCAGC GTCTGGAACA TTCTGGACCT GGTGGTCATC TTGCTCTCCA 
     2221 TCGTGGCTGT GGGTTTCCAC ATATTCCGAA CCCTGGAAGT GAACCGACTG ATGGGAAAGC 
     2281 TTCTGCAACA GCCAGACACG TATGCAGACT TTGAGTTCCT GGCCTTCTGG CAGACTCAGT 
     2341 ACAATAACAT GAACGCGGTC AACCTTTTCT TTGCTTGGAT CAAGATATTC AAGTATATCA 
     2401 GCTTCAACAA GACCATGACA CAGCTCTCCT CCACCCTGGC TCGCTGTGCC AAGGACATCC 
     2461 TGGGCTTTGC CATCATGTTC TTCATTGTCT TCTTCGCTTA CGCCCAGCTT GGCTACCTGC 
     2521 TTTTTGGGAC CCAAGTGGAA AACTTTAGCA CTTTCGTCAA GTGCATTTTC ACTCAGTTCC 
     2581 GGATAATCCT TGGGGATTTT GACTACAATG CCATCGACAA TGCCAACAGA ATCCTGGGCC 
     2641 CTGTGTACTT TGTCACCTAT GTCTTCTTCG TCTTCTTCGT GCTCCTGAAC ATGTTCCTGG 
     2701 CCATCATCAA CGACACATAC TCCGAGGTCA AGGAGGAGCT GGCTGGCCAG AAGGATCAGT 
     2761 TGCAGCTTTC TGACTTCCTG AAACAGAGCT ACAACAAGAC CCTACTAAGG CTGCGCCTGA 
     2821 GGAAAGAGCG GGTTTCTGAT GTGCAGAAGG TCCTGAAGGG TGGGGAACCA GAGATCCAGT 
     2881 TTGAAGATTT CACCAGCACC TTGAGGGAAC TGGGGCACGA GGAGCACGAG ATCACCGCTG 
     2941 CCTTCACCAG GTTTGATCAG GATGGGGACC ACATACTGGA TGAGGAGGAG CAGGAACAGA 
     3001 TGCGGCAGGG ACTGGAAGAG GAGAGGGTGA CCCTCAATGC TGAGATTGAG AACCTAGGCC 
     3061 GGTCTGTTGG ACACAGCCCC CCAGGCGAAT TGGGCGCGGA GGCTGCCAGA GGACAAAGCT 
     3121 GGGTTTCTGG AGAAGAATTC GACATGCTCA CAAGGAGAGT TCTGCAGCTG CAGTGTGTTC 
     3181 TGGAAGGAGT TGTGTCCCAG ATTGATGCTG TAGGCTCAAA GCTGAAGATG CTGGAGAGGA 
     3241 AAGGGGAGCT GGCTCCCTCC CCAGGAATGG GGGAACCAGC TGTTTGGGAG AACCTGTATA 
     3301 ATCCGTCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3361 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3421 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3481 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3541 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3601 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3661 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3721 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3781 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3841 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3901 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3961 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4021 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4081 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4141 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4201 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4261 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4321 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4381 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4441 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4501 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4561 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4621 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4681 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4741 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4801 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4861 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4921 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4981 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5041 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5101 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5161 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5221 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5281 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5341 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5401 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5461 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5521 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5581 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5641 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5701 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5761 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5821 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5881 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5941 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6001 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6061 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6121 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6181 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6241 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6301 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6361 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6421 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6481 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6541 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6601 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6661 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6721 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6781 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6841 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6901 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6961 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7021 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7081 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7141 CAAAAATTTA ACGCGAATTT T
//