LOCUS       MR225285L1                9084 bp    DNA     circular  SYN 
DEFINITION  Adam12 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 12 (meltrin alpha) (Adam12)
ACCESSION   NM_007400
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7375..7555 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8283..8942 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7714..8282 
                     /label="ColE1"  
     misc_feature    6250..6270 
                     /label="LR50 primer"  
     misc_feature    6047..6118 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6259..6850 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6029..6035 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6029 
                     /label="Adam12"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGAGCGC CCGGCGCGGC GCGCGCCCCC 
     3361 CGCCCGCGCC CTCCTGCTGG CCCTGGCTGG GGCCCTGCTG GCGCCCCGTG CAGCCCGAGG 
     3421 GATGAGTTTG TGGGACCAGA GAGGAACTTA CGAAGTGGCC AGAGCCTCCC TTCTGAGCAA 
     3481 GGACCCTGGG ATCCCAGGAC AGAGCATCCC AGCCAAGGAT CATCCAGACG TGCTGACTGT 
     3541 GCAACTGCAG CTGGAGAGCC GAGACCTGAT CCTCAGCCTG GAAAGGAATG AGGGACTCAT 
     3601 TGCCAATGGC TTCACGGAGA CCCATTATCT GCAAGATGGT ACTGATGTCT CTCTCACTCG 
     3661 AAATCACACG GATCATTGTT ACTACCATGG ACATGTGCAA GGAGATGCTG CATCAGTGGT 
     3721 CAGCCTCAGT ACTTGCTCTG GTCTCCGGGG ACTTATCATG TTTGAAAATA AAACGTACAG 
     3781 CTTAGAGCCA ATGAAAAACA CCACTGACAG CTACAAACTC GTCCCAGCTG AGAGCATGAC 
     3841 GAACATCCAA GGGCTGTGTG GGTCACAGCA TAACAAGTCC AACCTCACCA TGGAAGATGT 
     3901 CTCCCCTGGA ACCTCTCAAA TGCGGGCAAG AAGGCATAAG AGAGAGACCC TTAAGATGAC 
     3961 CAAGTACGTA GAGCTGGTTA TTGTGGCAGA CAACAGAGAG TTTCAGAGGC AAGGAAAAGA 
     4021 CCTGGAGAAA GTTAAGCAGC GATTAATAGA GATCGCCAAT CACGTTGACA AGTTTTACAG 
     4081 ACCACTGAAC ATCCGGATCG TGCTGGTAGG AGTGGAAGTG TGGAATGACA TCGACAAATG 
     4141 CTCTATAAGC CAGGACCCAT TCACCAGCCT CCATGAGTTT CTAGACTGGA GAAAGATAAA 
     4201 GCTTCTACCT CGAAAATCCC ACGACAATGC TCAGCTTATC AGTGGGGTTT ATTTCCAAGG 
     4261 AACCACCATC GGCATGGCAC CCATCATGAG CATGTGCACT GCAGAACAGT CTGGAGGAGT 
     4321 TGTCATGGAC CATTCAGACA GCCCCCTTGG TGCCGCAGTG ACCTTGGCAC ATGAGCTGGG 
     4381 CCACAACTTC GGGATGAACC ATGACACACT GGAGAGGGGC TGCAGCTGCA GAATGGCCGC 
     4441 AGAGAAAGGA GGCTGCATCA TGAACCCGTC CACGGGGTTC CCATTCCCCA TGGTGTTCAG 
     4501 CAGCTGCAGC AGGAAGGACC TGGAGGCTAG CCTGGAGAAG GGCATGGGGA TGTGCCTCTT 
     4561 CAACCTACCA GAGGTCAAGC AGGCCTTTGG GGGCCGGAAG TGTGGAAATG GCTATGTGGA 
     4621 AGAGGGAGAA GAGTGTGACT GCGGAGAACC GGAGGAATGC ACGAATCGCT GCTGTAACGC 
     4681 TACCACCTGT ACTCTGAAGC CAGATGCTGT GTGCGCGCAC GGGCAGTGCT GTGAAGACTG 
     4741 TCAGCTGAAG CCTCCAGGAA CTGCATGCAG GGGCTCCAGC AACTCCTGTG ACCTCCCAGA 
     4801 ATTCTGCACA GGGACTGCCC CTCACTGTCC AGCCAATGTG TACCTACATG ATGGCCACCC 
     4861 GTGTCAGGGC GTGGATGGTT ACTGCTACAA CGGCATCTGC CAGACCCATG AGCAGCAGTG 
     4921 TGTCACGCTC TGGGGACCAG GTGCTAAACC GGCTCCTGGC ATCTGCTTTG AGCGAGTCAA 
     4981 CTCTGCAGGA GATCCTTATG GTAACTGTGG CAAAGACTCC AAGAGCGCCT TCGCCAAATG 
     5041 TGAGCTGAGA GATGCCAAGT GTGGGAAAAT CCAGTGTCAA GGTGGTGCAA GCCGACCTGT 
     5101 CATTGGTACC AATGCTGTTT CCATAGAAAC AAATATCCCA CAGCAGGAAG GAGGTCGGAT 
     5161 TCTGTGCCGG GGGACCCATG TGTACTTGGG TGATGACATG CCAGACCCAG GGCTTGTGCT 
     5221 TGCAGGAACA AAGTGTGCAG AAGGAAAAAT CTGCCTCAAT CGTCGATGTC AGAATATCAG 
     5281 TGTCTTCGGC GTTCACAAGT GTGCCATGCA GTGCCACGGC CGAGGGGTAT GTAACAACAG 
     5341 GAAGAATTGC CACTGTGAAG CCCACTGGGC TCCACCCTTC TGTGACAAGT TTGGCTTTGG 
     5401 AGGAAGCACA GACAGTGGTC CCATCAGGCA AGCAGATAAC CAGGGCTTGA CTGTAGGAAT 
     5461 CCTGGTGAGC ATCCTGTGTC TGCTTGCTGC TGGATTTGTG GTGTATCTCA AAAGGAAGAC 
     5521 GTTGATGCGG CTGCTGTTCA CACATAAAAA AACCACCATG GAAAAGCTAA GGTGTGTGCA 
     5581 CCCTTCCCGG ACACCCAGTG GCCCTCACCT TGGCCAGGCT CACCACACCC CCGGGAAAGG 
     5641 CCTGCTGATG AACCGGGCAC CACATTTCAA TACCCCCAAG GACAGGCACT CGCTGAAATG 
     5701 CCAGAACATG GACATCAGCA GGCCCCTCGA CGCTCGAGCC GTCCCACAGC TTCAGTCACC 
     5761 TCAGCGAGTG CTCCTGCCTC TCCACCAGAC CCCACGTGCA CCCAGTGGCC CTGCCAGGCC 
     5821 CCTGCCCGCC AGTCCTGCAG TCAGGCAGGC CCAGGGCATT CGAAAACCCA GTCCTCCTCA 
     5881 GAAGCCTCTG CCTGCTGATC CACTGAGCAG GACTTCTCGG CTCACTAGTG CCTTGGTGAG 
     5941 GACCCCAGGG CAGCAGGAAC CTGGGCACCG CCCAGCCCCC ATCAGACCTG CCCCTAAGCA 
     6001 TCAAGTACCC AGACCTTCCC ACAATGCCTA TATCAAGACG CGTACGCGGC CGCTCGAGCA 
     6061 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     6121 CGATAAGGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6181 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6241 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6301 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6361 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6421 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6481 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6541 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6601 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     6661 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     6721 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     6781 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     6841 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     6901 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     6961 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7021 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7081 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7141 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7201 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7261 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7321 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7381 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7441 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7501 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7561 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7621 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     7681 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     7741 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     7801 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     7861 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     7921 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     7981 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8041 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8101 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8161 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8221 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8281 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8341 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8401 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8461 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8521 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8581 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     8641 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     8701 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     8761 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     8821 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     8881 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     8941 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9001 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT CCCGCGCACA 
     9061 TTTCCCCGAA AAGTGCCACC TGAC
//