LOCUS       MR225227                9510 bp    DNA     circular  SYN 
DEFINITION  Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 1
ACCESSION   NM_001194940
VERSION     NM_001194940.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5667..5758 
                     /label="Myc-DDK"  
     misc_feature    5788..6373 
                     /label="PolyA signal"  
     misc_feature    5845..5864 
                     /label="Restriction Enzyme"  
     misc_feature    6649..7321 
                     /label="ColE1"  
     misc_feature    7751..8545 
                     /label="Kan/Neo"  
     misc_feature    8580..8882 
                     /label="SV40 ori"  
     misc_feature    8968..9445 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5649..5655 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5649 
                     /label="Dlc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGTAGCT ATCAGAAAGA GGAGCTGGGA AGAGCACGTG ACCCAGCAGA 
     1081 CCGAACAGCC TTTTATTTCT GATGAGTATG ACATAGCATG TCACCACGGA CTGGCATCCG 
     1141 ACAGCTTGGA GGGAAGGATG GAAAAAGATG CGACCTTAAA TGTGGACCAT AAAGAGAAGT 
     1201 GTGCCTCTCT ACCGGACCGC TGTTGTGGGT CAGAGACAAG AGACTTTCCT GGGAGGCCAA 
     1261 TGGGTCACAT TGCACAGGAG GTAGATGAAA GTGACAGCCA AGAAGGGGAA GAGCGGTTTC 
     1321 TATCGCTCGA GGCCAGCACA GAAACACTAG TGCACATTTC GGATGAGGAT ACTGATTCTG 
     1381 ATCTAAACCT TATCAATGAC TCACAGATTT TAACTCCGCA AAGACATGAA AGAGACAGCC 
     1441 AAGATAGTGT CAAAGGAGCA GGCTCCTTCA TGAAGACACT GTCCACCATG CAAAGTAGCC 
     1501 AAGATTCTCA TAACACCTGG AGGATGGCTG GTAAGGCTGA TATGTCTCCA GCAGAAGGAA 
     1561 GTGGAGAAAG GAAAGATGTT GAGGCTATAA ATAAAAGCCT GGGGCTTTGT AAGAATACGA 
     1621 GTGCAGATGA AACAAAAGAT GTGCCCATAG GAAATACATT CCAGTCTTTG AATACGGAAG 
     1681 ATGACGTCAC TCCTAAGACA CTACTACTTG ATTCTTCGGT ACTCATAGAA GATGTCACAC 
     1741 CTGAGACACA ACTGCTTGAT TCTGCAGTCA TCACTCAACA ATGCAGGAAA TCTGACTTCC 
     1801 CTAAAGATGA CCAGGAAAAT AATCGGCACC ATGTCTTAGA AGAAGACGTC TTAGCAGCTC 
     1861 CTTATGTAGA CAGAGGGCTG CCATTATTAA AAGCAGATTG CAGAAGCAGC CTTCTTCAGC 
     1921 CTCCTTCCTG CCCTGGTGGG ATGTCAGCAG AAAATGACCT GGAGAAGAGT GGATTCTCAG 
     1981 AATATCAAAA CAAAAGCACT CTCGGGGTCA GCAGGGAAGA TGGCATGCAG TGTTTACACT 
     2041 TAAGGGGCCC CGTGACCACC CAGGAGACTG TAGACAATCA AGTCAGACTG CGCAAGAGAA 
     2101 AGGAAACAAG GGAAGATCGA GACAGGACAC GGCTGGACTC CATGGTGCTG CTCATTATGA 
     2161 AACTGGACCA ACTAGACCAA GACATAGAGA ATGCTCTCAG CACTGCCTCC TCTCCATCCA 
     2221 GCACCCCAAC GAACCTGCGG CGGCATGTCC CTGATCTGGA ATCAGGATCT GAAAGCAGAA 
     2281 CAGACAGTAT GGCAGTAAAT CAGACACAGG TGAATCTGTC TTCAGAGTCC ACCGATCCAT 
     2341 CATCCACTCC AGGGAGCAAT TCTGGAACCA AACCCAAAGC CATGGCTGCT CCGTGTGTTG 
     2401 GAGAAAAGGA AATGGCTGAA ATTGAAGCCA AGGAAGCTTG CGACTGGCTG AGAGTGACTG 
     2461 GTTTCCCCCA GTATGCACAG CTGTATGAAG ATCTACTGTT CCCTATTGAT ATTGCGCTGG 
     2521 TCAAGAGAGA ACATGACTTC TTGGACAGAG ACGCCATCGA GGCTCTCTGC AGGCGCTTAA 
     2581 ATACTCTAAA CAAGTGTGCA GTCATGAAGC TGGAGATCAG TCCTCACCGG AAGCGAAGTG 
     2641 AGGATTCGGA TGAAGATGAG CCTTGCGCCA TAAGCGGCAA GTGGACTTTC CAGAGGGACA 
     2701 GCAAGCGGTG GTCCCGCCTT GAAGAGTTTG ACGTCTTTTC CCCAAAGCAG GATCCAATCC 
     2761 CTGGGTCCCC AGACAACTCT CGTTTGCAAA GCGCCACAAG CCACGAAAGC ATGCTGACAG 
     2821 ACCTCAGCGA GCACCAGGAG GTGGCCTCTG TCCGAAGCCT CAGCAGCACC AGCAGCAGCG 
     2881 TCCCCACCCA CGCAGCCCAC AGTGGAGATG CCACTACGCC CCGAACCAAT TCCGTCATCA 
     2941 GCGTCTGCTC CTCCGGACAC TTTGTAGGCA ATGATGACTC TTTTTCCAGC CTGCCGTCTC 
     3001 CCAAGGAACT GTCCAGCTTC AGTTTTAGCA TGAAAGGCCA CCACGAGAAG AACACCAAGT 
     3061 CGAAGACGCG GAGCCTGCTC AAACGCATGG AGAGCCTGAA GCTCAAGGGC TCCCACCACA 
     3121 GCAAGCACAA GGCGCCTTCC AAGCTGGGGT TGATCATCAG TGCTCCCATT CTGCAGGAGG 
     3181 GTATGGATGA GGCAAAGCTG AAGCAGCTGA ACTGTGTGGA GATCTCAGCC CTCAATGGCA 
     3241 ACCACATCAA CGTGCCCATG GTACGGAAAA GGAGCGTGTC TAACTCCACC CAGACCAGCA 
     3301 GCAGCAGCAG CCAATCAGAG ACCAGCAGCG CGGTCAGCAC ACCCAGCCCG GTCACCAGGA 
     3361 CCCGGAGCCT CAGCACCTGT AACAAGCGGG TGGGCATGTA TCTAGAGGGC TTCGACCCAT 
     3421 TCAGTCAGTC CACCTTGAAC AACGTGACGG AGCAGAACTA TAAAAACCGT GAGAGCTACC 
     3481 CAGAGGACAC GGTGTTCTAC ATTCCCGAAG ATCACAAGCC CGGCACCTTC CCTAAGGCCC 
     3541 TCTCCCATGG CAGTTTCTGT CCCTCGGGAA ACAGTTCTGT GAACTGGAGG ACCGGAAGCT 
     3601 TCCATGGCCC TGGCCATCTC AGCCTACGGA GAGAAAACAG CCATGACAGT CCTAAGGAGC 
     3661 TGAAGAGACG CAATTCTTCC AGCTCTCTGA GCAGCCGCCT GAGCATCTAT GATAACGTAC 
     3721 CGGGTTCTAT CCTGTACTCC AGCTCGGGAG AACTGGCCGA CCTGGAGAAT GAGGACATCT 
     3781 TCCCTGAGCT GGATGACATT CTCTACCACG TGAAGGGGAT GCAGCGGATA GTCAACCAGT 
     3841 GGTCCGAGAA GTTTTCCGAC GAGGGAGACT CGGACTCAGC CCTGGACTCT GTCTCTCCTT 
     3901 GCCCGTCATC TCCAAAACAG ATACACCTGG ATGTGGACCA TGACCGAAGG ACACCCAGTG 
     3961 ACCTGGACAG CACAGGCAAC TCCCTCAATG AGCCCGAAGA GCCCACTGAT ATCCCGGAAA 
     4021 GAAGAGACTC AGGGGTGGGG GCTTCCCTGA CCAGGTGCAA TAGGCACAGA CTGAGGTGGC 
     4081 ACAGCTTCCA GAGCTCCCAC CGGCCGAGCC TCAACTCCGT GTCACTGCAG ATTAACTGCC 
     4141 AGTCTGTGGC CCAGATGAAC CTGCTGCAGA AATACTCGCT CCTGAAATTG ACGGCCCTGC 
     4201 TGGAAAAGTA CACGCCTTCC AACAAGCACG GCTTCAGCTG GGCTGTGCCC AAGTTCATGA 
     4261 AAAGGATCAA GGTTCCAGAC TACAAGGACC GGAGTGTGTT TGGGGTCCCA CTGACTGTGA 
     4321 ACGTGCAGCG CTCAGGACAG CCCCTGCCCC AGAGCATCCA GCAGGCCATG CGCTACCTCC 
     4381 GTAACCACTG TCTGGACCAG GTCGGGCTCT TCAGGAAGTC AGGTGTCAAA TCCCGGATCC 
     4441 AGGCTCTACG CCAGATGAAT GAAAGCGCTG AAGATAATGT CAACTATGAA GGCCAGTCTG 
     4501 CTTATGATGT GGCAGACATG TTAAAGCAAT ATTTTCGAGA TCTTCCTGAG CCCCTCATGA 
     4561 CGAACAAACT CTCCGAAACC TTCCTGCAGA TCTACCAGTA TGTTCCCAAG GACCAGCGCC 
     4621 TCCAAGCCAT CAAGGCGGCC ATTATGCTCC TGCCCGACGA GAACCGGGAG GTTCTGCAGA 
     4681 CACTTCTCTA TTTCCTGAGC GATGTCACAG CGGCTGTGAA AGAAAACCAG ATGACTCCCA 
     4741 CCAACCTGGC TGTGTGCCTA GCTCCGTCCC TCTTCCACCT CAACACCCTG AAGCGAGAGA 
     4801 ATTCTTCTCC AAGGGTAATG CAAAGAAAAC AGAGTTTGGG CAAACCAGAC CAGAAAGACC 
     4861 TGAATGAAAA CCTAGCGGCG ACTCAAGGGC TGGCCCACAT GATTGCTGAG TGCAAGAAGC 
     4921 TCTTCCAGGT CCCTGAGGAA ATGAGCCGGT GCCGTAACTC CTACACTGAA CAAGAGCTGA 
     4981 AGCCCCTTAC CCTGGAGGCC TTGGGACACC TGAATAGTGA CCAGCCTGCT GACTACAGAC 
     5041 ACTTCCTCCA GGACTGTGTG GATGGCCTGT TTAAGGAGGT CAAAGAGAAG TTCAAAGGCT 
     5101 GGGTCAGCTA CCCCACCTCC GAACAGGCTG AGCTGTCCTA TAAGAAGGTC AGCGAAGGAC 
     5161 CCCCGTTAAG GCTTTGGAGG TCAACTATCG AAGTCCCCGC TGCACCCGAG GAGATCTTAA 
     5221 AGCGCCTTCT GAAGGAGCAA CACCTCTGGG ATGTGGACCT GCTGGACTCC AAGGTGATTG 
     5281 AAATCCTGGA CAGCCAGACT GAAATCTACC AATACGTCCA AAACAGCATG GCGCCCCACC 
     5341 CTGCGCGGGA CTACGTCGTA TTGAGGACCT GGAGGACTAA CTTACCCAGG GGGGCCTGTG 
     5401 CTCTCTTACT CACCTCTGTG GATCACGACC GGGCCCCCGT GGCGGGGGTG AGGGTTAATG 
     5461 TGCTCCTGTC CAGATATTTG ATTGAACCCT GCGGGTCAGG AAAATCCAAG CTTACCTACA 
     5521 TGTGCAGAGC TGATTTAAGG GGCCACATGC CGGAGTGGTA CTCAAAATCT TTTGGACATT 
     5581 TGTGTGCAGC CGAAGTGGTA AAGATCCGAG ACTCCTTCAG TAATCAGAAC ACTGAAAGCA 
     5641 AGGACACGAG ATCTAGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5701 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5761 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5821 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5881 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5941 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     6001 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     6061 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6121 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6181 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6241 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6301 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6361 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6421 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6481 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6541 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6601 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6661 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6721 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6781 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6841 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6901 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6961 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     7021 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     7081 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7141 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7201 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7261 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7321 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7381 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7441 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7501 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7561 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7621 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7681 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7741 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7801 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7861 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7921 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7981 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     8041 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     8101 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8161 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8221 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8281 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8341 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8401 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8461 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8521 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8581 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8641 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8701 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8761 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8821 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8881 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8941 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     9001 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     9061 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9121 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9181 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9241 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9301 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9361 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9421 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9481 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//