LOCUS       MR224951                7494 bp    DNA     circular  SYN 
DEFINITION  Espn (Myc-DDK-tagged) - Mouse espin (Espn), transcript variant 1
ACCESSION   NM_207687
VERSION     NM_207687.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3651..3742 
                     /label="Myc-DDK"  
     misc_feature    3772..4357 
                     /label="PolyA signal"  
     misc_feature    3829..3848 
                     /label="Restriction Enzyme"  
     misc_feature    4633..5305 
                     /label="ColE1"  
     misc_feature    5735..6529 
                     /label="Kan/Neo"  
     misc_feature    6564..6866 
                     /label="SV40 ori"  
     misc_feature    6952..7429 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3633..3639 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3633 
                     /label="Espn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTGGAG CAGGCGCTGC AGGCGGCACG GCGGGGCGAC CTGGACGTGC 
     1081 TGAGGTCCCT GCACGCCGCC GGCCTGCTGG GGCCTTCTCT GCGCGACTCG CTAGACGCCC 
     1141 TGCCGGTGCA CCATGCGGCC CGCTCAGGCA AGCTGCACTG TCTGCGCTAC TTGGTGGAGG 
     1201 AGGTTGCCCT CCCAGCCGTG TCCCGCGCGC GCAACGGCGC CACACCAGCC CATGACGCCG 
     1261 CCGCCACAGG CTACCTCTCT TGCCTGCAGT GGCTGCTCAC ACAGGGTGGC TGCAGGGTGC 
     1321 AGGAAAAAGA TAACTCTGGT GCCACAGTCC TGCATCTGGC TGCCCGCTTT GGCCACCCGG 
     1381 ATGTGGTGAA GTGGCTGCTG TACCAGGGCG GTGCAAATTC GGCCATCACC ACAGACACGG 
     1441 GCGCCCTGCC TATCCACTAC GCTGCCGCCA AAGGAGACCT CCCCTCGCTG AAGCTTCTGG 
     1501 TCGGGCATTA CCCTGAGGGA GTGAATGCCC AAACCAACAA CGGTGCCACG CCCCTGTACC 
     1561 TGGCGTGCCA GGAGGGCCAC CTGGAAGTGA CGAAGTACCT TGTGCAGGAG TGCAGTGCAG 
     1621 ATCCGCACCT GCGCGCCCAA GACGGCATGA CACCCCTACA TGCCGCAGCA CAGATGGGCC 
     1681 ACAACCCAGT CTTGGTGTGG CTGGTGAGCT TTGCAGACGT TAGCTTTTCA GAGCAGGACC 
     1741 ACGACGGCGC CACGGCCATG CACTTTGCTG CCAGTCGCGG CCACACCAAA GTGCTCAGCT 
     1801 GGCTCCTGCT GCACGGGGCA GAGATCTCGC AGGACCTGTG GGGCGGAACC CCGCTGCATG 
     1861 ACGCTGCTGA GAACGGGGAA CTGGAGTGCT GCCAGATCCT CGCGGTGAAC GGCGCTGGGC 
     1921 TGGACGTCCG CGACCACGAT GGGTACACCG CTGCCGACCT GGCAGAGTTC AATGGCCACA 
     1981 CCCACTGTTC CCGCTACCTA CGTACGGTGC AAACCCTGAG CTTGGAACAC CGAGTCTTGT 
     2041 CCCGGGATCA ATCCATGGAC CTGGAGGCAA AGCAGCTGGA CTCGGGTATG TCCTCGCCCA 
     2101 ACACCACCAT GTCGGTCCAG CCAATGACCT TTGACCTGGG CTCGCCTACT AGCACGTTCT 
     2161 CCAACTATGA CTCCTGCTCC TCCAGTCACT CCAGCAGCAA GGGGCAGCGA TCGAATCGAG 
     2221 GGATTCCAGG TGCAAGAGCT GCAGACTTAC AGAGCTACAT GGACATGCTG AACCCAGAGA 
     2281 AGAGCTTGCC TCGGGGCAAG CTAGGGAAGC CTTCCCCGCC ACCACCTCCA CCACCACCAC 
     2341 CACCAAGCTT CCCGCCACCC CCACCACCCA CAGGCACCCA GCCGCCCCCA CCTCCACCAG 
     2401 GCTACCCAGC TCCCAATCCC CCTGTGGGAC TGCATCTGAA TAACATTTAC ATGCAGACCA 
     2461 AGAACAAGCT TCGCCATGTG GAGGTGGACT CGCTCAAGGA GCCCAAGGTG GAGCTGAACG 
     2521 ATCAGTTTGC ACAGCCGAGC TCGGGCGACG GCCACTCGGG GCTACACAGG CAGGACTCCG 
     2581 GGCTGCTCAG GCAGGATTCG GAGCTGCTGC ACAGGCAGGA GCTGCTCAGG CACAGCACCG 
     2641 GACTGCGCAG GCAGGACTCC GACCGCAAAC AGCGCTCGTT CAGTAAACAG CCCAGCACGG 
     2701 GGGACTACTA CCGCCAGCTG GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA 
     2761 TGGCCCACAG CGAGGAGGCG GCGCTGCTCC CCGGGAACCA CGTGCACAAC GGCTGCTCAG 
     2821 CGGACTCCAA AGCGTCCAGG GAGCTGCCGC CGCCACCGCC GCCGCCGCCG CTGCCCGAGG 
     2881 CCCTGAGTTC GCCGCCGCCC GCCCCACCTC TGCCCATCGA GGGCGCGGGC GCAGCCTGCG 
     2941 GGCAGCGTCG TTCCTCGTCT TCTACTGGCA AAGTGAGAGT CCTGAGACAC AGGAAGAGCA 
     3001 CCAAATCTTT CAACATGATG TCCCCAACGG GTGATAACTC AGAGCTTCTG GCTGAGATAA 
     3061 AGGCGGGCAA GAGCCTGAAG CCGACACCGC AGAGCAAGGG GCTGACAACC GTGTTCTCAG 
     3121 GCAGTGGGCA GCCAGCCTCC CAGCCTGAGT CACCGCAGCC TCTGGTGTCA CCTGCGCCAT 
     3181 CTCGGACTCG GAGCCCCACC CCGCCAGCCT CTGGGTCTCA GCCACTGCTC AATGGCAGTG 
     3241 TGGTGCCGGC ACCACCTGCC ACCCCGGCAC CTGGAGTCCA TCTGGATGTG GAGGCCCTCA 
     3301 TTCCCACTCT TGATGAGCAG GGCCGGCCCA TCCCGGAGTG GAAGCGCCAG GTGATGGTCC 
     3361 GCAAGCTGCA GCAGAAGATG CAGGAGGAAG AGGAGCAGCG GAGGAAGGAG GAAGAGGAGG 
     3421 AGGCCCGGCT CGCCAGCCTG CCTGCCTGGA GACGAGACAT TCTTCGGAAG AAGCTGGAGG 
     3481 AGGAGAGGGA GCAGAAGCGA AAAGAGGAGG AGCGGCAAAA GCTGGAGGAA ATACAGAGGG 
     3541 CGAAAGAACA GTCGGAGAAG CTGCGGACAC TAGGCTACGA CGAAGCCAAG CTCGCGCCCT 
     3601 GGCAGCGACA GGTCATCTTG AAGAAGGGGG AGATCCCTAA GACGCGTACG CGGCCGCTCG 
     3661 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3721 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3781 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3841 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3901 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3961 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4021 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4081 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4141 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4201 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4261 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4321 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4381 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4441 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4501 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4561 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4621 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4681 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4741 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4801 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4861 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4921 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4981 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5041 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5101 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5161 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5221 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5281 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5341 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5401 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5461 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5521 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5581 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5641 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5701 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5761 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5821 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5881 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5941 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6001 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6061 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6121 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6181 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6241 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6301 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6361 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6421 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6481 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6541 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6601 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6661 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6721 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6781 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6841 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6901 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6961 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7021 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7081 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7141 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7201 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7261 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7321 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7381 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7441 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//