LOCUS       MR224702                7716 bp    DNA     circular  SYN 
DEFINITION  Dspp (Myc-DDK-tagged) - Mouse dentin sialophosphoprotein (Dspp). Note: ORF is codon optimized
ACCESSION   NM_010080
VERSION     NM_010080.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3873..3964 
                     /label="Myc-DDK"  
     misc_feature    3994..4579 
                     /label="PolyA signal"  
     misc_feature    4051..4070 
                     /label="Restriction Enzyme"  
     misc_feature    4855..5527 
                     /label="ColE1"  
     misc_feature    5957..6751 
                     /label="Kan/Neo"  
     misc_feature    6786..7088 
                     /label="SV40 ori"  
     misc_feature    7174..7651 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="Dspp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATGAAA ATCATTATCT ATATTTGTAT CTGGGCAACC GCTTGGGCGA 
     1081 TCCCGGTCCC CCAGCTTGTA CCCCTGGAGC GCGACATTGT CGAAAACTCC GTGGCCGTAC 
     1141 CACTTCTGAC CCACCCAGGA ACGGCCGCAC AGAATGAGCT TTCCATAAAT TCCACCACTA 
     1201 GCAATAGCAA CGATTCCCCA GATGGTAGTG AGATTGGTGA ACAGGTACTT TCCGAGGACG 
     1261 GCTACAAGAG GGACGGAAAC GGATCTGAGT CCATACACGT GGGGGGTAAG GACTTTCCAA 
     1321 CCCAGCCTAT CCTGGTGAAT GAACAGGGGA ATACGGCAGA AGAACACAAC GATATTGAGA 
     1381 CGTATGGGCA TGACGGAGTC CACGCTCGCG GCGAGAATTC CACAGCGAAC GGTATACGGT 
     1441 CCCAGGTCGG AATTGTTGAG AATGCTGAAG AAGCCGAATC CTCCGTGCAT GGGCAGGCAG 
     1501 GTCAAAACAC CAAAAGCGGA GGAGCTAGTG ATGTATCCCA GAACGGAGAC GCAACGCTTG 
     1561 TTCAGGAGAA CGAACCCCCA GAGGCCAGCA TAAAGAATTC CACAAACCAC GAGGCTGGGA 
     1621 TCCACGGAAG CGGTGTCGCA ACACACGAGA CTACGCCCCA GCGCGAAGGA CTTGGGTCTG 
     1681 AGAATCAGGG GACTGAGGTT ACCCCAAGTA TCGGCGAGGA CGCGGGTCTG GATGATACCG 
     1741 ATGGGAGCCC TTCCGGCAAC GGGGTGGAGG AGGATGAAGA TACGGGCTCT GGCGATGGGG 
     1801 AGGGTGCCGA AGCGGGCGAC GGAAGGGAAT CTCACGACGG CACTAAAGGC CAAGGTGGGC 
     1861 AGTCCCACGG GGGCAATACC GATCACCGCG GGCAGAGTAG TGTATCTACT GAGGATGACG 
     1921 ACTCTAAAGA GCAGGAGGGT TTCCCGAATG GGCATAACGG CGACAATAGC TCCGAGGAGA 
     1981 ATGGGGTGGA GGAAGGGGAT AGCACTCAGG CCACCCAAGA TAACCAAAAG CTGAGTCCCA 
     2041 AAGATACTCG AGATGCCGAG GGGGGGATCA TCAGTCAGAG CGAAGCCTGT CCGTCTGGGA 
     2101 AATCTCAGGA TCAAGGCATT GAGACAGAGG GTCCGAATAA GGGCAACAAG AGCATTATTA 
     2161 CCAAAGAAAG TGGCAAGCTC TCCGGAAGTA AAGATAGCAA TGGCCATCAG GGCGTTGAGC 
     2221 TGGACAAACG CAACAGCCCT AAGCAGGGGG AGTCCGACAA ACCGCAGGGG ACCGCGGAAA 
     2281 AGTCAGCTGC TCACAGCAAT CTCGGCCACA GCAGGATAGG ATCAAGCTCA AATAGTGATG 
     2341 GGCACGATTC CTATGAATTC GACGACGAGT CTATGCAGGG GGACGATCCT AAATCATCCG 
     2401 ATGAAAGTAA CGGCAGCGAC GAATCCGACA CTAACAGTGA ATCAGCCAAC GAGAGCGGAT 
     2461 CTAGAGGGGA CGCTTCATAC ACTTCCGATG AATCATCTGA CGACGATAAC GATTCTGATA 
     2521 GTCATGCTGG CGAAGATGAT TCATCTGATG ATTCCAGCGA CACTGACGAT AGCGACTCCA 
     2581 ACGGGGACGG GGACAGCGAT AGTAACGGGG ACGGAGACAG CGAGAGCGAA GATAAGGATG 
     2641 AGTCCGATTC ATCCGACCAT GACAACTCCA GTGATAGTGA GTCCAAGTCC GACTCTTCCG 
     2701 ATTCCAGCGA CGATAGCTCA GATTCTTCCG ACTCCTCTGA TAGTAGCGAT TCTTCTGACT 
     2761 CCTCAGATAG CTCTGACAGC AGCGACTCCA GTGACAGCTC CGATTCTAAC AGCTCCAGCG 
     2821 ACTCCTCCGA CAGCAGTTCT TCAAGTGATT CATCTGATTC CTCAGATAGC AGTGACAGCT 
     2881 CTGATAGCTC AGACAGCTCT GATAGCAGCG ACTCTAGCGA TTCCAGCGAT TCATCTGACA 
     2941 GTAGTGACTC CAGCGACTCA TCCGACAGTA GTGACTCCTC CAGCTCATCA GACTCATCTG 
     3001 ATTCCAGTAG TTCTTCAGAT TCCTCCGACA GTAGCGATAG CTCCGATTCT AGTGACTCCT 
     3061 CCGACTCCAG CGACTCCAGT TCAAGTGACA GCAGTAGCAG CTCCAATAGT AGCGACTCCA 
     3121 GTGACTCCAG TGACTCCTCA TCAAGCAGCG ACTCTTCCAA TTCATCCGAC TCTTCCGACA 
     3181 GTTCAGATAG TAGCGATTCC TCCGACTCCT CAGACAGCTC CAATTCTAGC GATAGTTCCG 
     3241 ATTCCTCCAG CAGCTCTGAC TCCTCAGATT CCTCTTCCTC CTCCGATTCT TCCGATAGTA 
     3301 GTGATAGTTC TGACAGCTCC GAGTCCTCTG AGAGTAGTGA TTCTTCTAAT AGCAGCGACT 
     3361 CCAGTGACTC TTCCGATTCT TCTGATTCAA GCGATTCTAG CGACAGTAGC GACTCTAGCG 
     3421 ACTCCAGCGA CAGTTCAAAC AGTTCCGACA GTAGCGATAG CAGCGACTCT TCCGATAGCA 
     3481 GTGACTCTAG TAACTCTTCA GACTCAAGTG ATAGTTCTGA CTCAAGCGAT TCAAGCGACA 
     3541 GTTCAGATAG CTCTGATAGT TCTGACTCCA GTGATAGTAG CGACTCATCC GACAGCTCTG 
     3601 ATTCATCTGA TAGCTCCGAC TCCTCCGATA GTTCAGACAG TAGCGATAGT AGCGACTCAA 
     3661 GCGACAGCAG CGATTCCTCC GACTCCTCAG ATAGTAGCAA CAGTTCCGAC TCTTCTGACA 
     3721 GCGATTCCAA GGATTCTTCT AGTGATTCTT CTGACGGGGA CAGTAAAAGT GGCAATGGCA 
     3781 ACAGCGATTC CAATAGTGAC TCAAATTCCG ACTCTGACTC CGACAGCGAG GGGAGCGACT 
     3841 CTAATCACAG CACGTCCGAC GACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3901 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3961 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4021 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4081 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4141 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4201 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4261 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4321 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4381 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4441 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4501 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4561 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4621 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4681 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4741 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4801 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4861 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4921 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4981 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5041 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5101 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5161 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5221 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5281 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5341 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5401 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5461 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5521 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5581 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5641 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5701 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5761 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5821 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5881 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5941 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6001 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6061 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6121 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6181 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6241 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6301 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6361 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6421 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6481 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6541 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6601 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6661 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6721 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6781 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6841 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6901 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6961 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7141 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7201 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7261 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7321 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7381 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7441 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7501 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7561 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7621 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7681 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//