LOCUS       MR224565                8511 bp    DNA     circular  SYN 
DEFINITION  Mmrn1 (Myc-DDK-tagged) - Mouse multimerin 1 (Mmrn1), transcript variant 1
ACCESSION   NM_027613
VERSION     NM_027613.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="Mmrn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGGACTA AAGTTTCTTG TCCTGCTTTC TATTTTATGG GGCAGGGTCT 
     1081 TCAGGCTTAC CAACACTCAA CATTCATGGA CTGCGCCTAA GGATGAAGAC GCCTCACTGA 
     1141 CCCCGAACCC CGCTTCAGCT TCTGTAAGTG AAATACTGAG CCTCCAAGTG CTCTCGGCCA 
     1201 CTCAGAACCC CTCCACTCAG GGTCCTGCAG CTGCTGAGAG ATCTTCTGAA GACGATGTTC 
     1261 TCCTACAGTC AACATCACAG CCCTCTGAGA CAAGCACCCC TCCTGACGGG AGAAGCCAAA 
     1321 CATCCCTAGA GAAGACAGGG ACAGCAGTGG TATCCCTGCC TCTCTCTCTT CAGGACAAAC 
     1381 CCAGCATCAA GCCCAGCACC GGAGCAGGAA CTGTCATGCT TGCTAATGCC ACGTTGAAAT 
     1441 TCCTTCAGAG TTTTTCCAGA AAGTCTGATC AACAAGAAGT CTCCACTAAG TCGGCTGGCG 
     1501 ACATGGGAAA CAGGTCTGCA AGGGAAACAC ACCTCAGGAG AAGTGACAAC AGCAGAAATC 
     1561 AAAGACCCAG CTATCAAAAA CCCAGTTTTG AAACAACTAG AGGGAAGAAT TGGTGTGCTC 
     1621 ATGTTCATAC CAAGTTATCC CCGACAGTGA TACTGGACAC TGGCTCTCAT TTACCAAGTG 
     1681 GAAGAGGTTC TTGTGGCTGG TACAGCAGTG GACTCTGTTC CAGAAGATCT CAGAAGACAT 
     1741 CCAATGCTGT CTATAGAATG CAGCATAAGA TTGTCACCTC ATTAGAATGG AGATGCTGTC 
     1801 CTGGATACAT AGGTCCAAAC TGTCAGCTCA AAGTGGAAGA ACAGCAGCAG CTGGCACACA 
     1861 GTAACCAGGC TGAAAGTCAC ACCGCTGTTG ACCAAGGAAG AGCTCAGCAG CAGAAGCAAG 
     1921 ACTGTGGTGA CCCAGCCATG ATTCAAAAAC TGGCAGAGCA GCTGAGCCAA CAGGAGAGGA 
     1981 AGCTGTCGCT TCTGCAGAAG AAGGTTGACA ATGCTTCTCT GGTGGCAGAT GACATGAGGA 
     2041 ATGCATACCT CTCACTAGAA GGGAAAGTTG GAGAGGACAA CAGCAGGCAA TTTCAATCTT 
     2101 TTCTAAAAGC TCTAAAATCA AAGAGTATTG AGGATCTGCT AAAGAACATC GTAAAAGAAC 
     2161 AATTTAAAGT CTTCCAAGAT GATATGCAGG AGACGACTGC CCAGATCTTC AAGACTGTGT 
     2221 CAAGTCTATC AGAGGACTTG GAAAGTACCA GGCAGGCAGT TCTACAAGTG AACCAGTCCT 
     2281 TTGTTTCATC AACAGCCCAG AAAGACTTTG CTTTCATGCA AGAAAATCAG CCCACGTGGA 
     2341 AGGACATCAC AGATCTAAAG AACAGCATCA TGAACATCAG GCAGGAAATG GCCTTGACCT 
     2401 GTGAGAAGCC TGTCAAGGAA CTGGAAGCAA AACAAGCTCA TTTGGAAGGA GCACTAAGAC 
     2461 AGGAGCACTC ACAGATTGTT CTGTACCACC AATCCCTCAA TGAAACTCTC TCTAAAATGC 
     2521 AGGAAGCACA CACACAGCTG TTATCAGTTC TACAAGTATC AGGAACAGAG AATGTCGCCA 
     2581 CTGAGGAATC CCTCAACAGT AATGTCACTA AGTATATATC TGTACTACAG GAGACAGCAA 
     2641 GCAAGCAAGG TCTGATGCTG CTGCAGATGC TCAGTGATTT GCATGTCCAA GAGAGCAAGA 
     2701 TCAGCAACCT TACCATCCTT TTGGAGATGG AGAAAGAATC AGCCAGGGGA GAATGTGAGG 
     2761 AAATGCTATC TAAATGCAGG CATGACTTTA AATTTCAACT CAAGGACACA GAGGAAAATC 
     2821 TGCATGTGTT AAATCAAACA CTGTCTGAGG TTATCTTTCC CATGGACATC AAGGTGGATA 
     2881 AAATGAGTGA GCAGCTGAAT GATTTGACAT ATGACATGGA GATCCTTCAG CCTTTGCTGG 
     2941 AGCAGAGGTC GTCGCTTCAA CACCAGGTTA TACACAAGCC AAAGGAAGCC ACAGTTACTC 
     3001 GCAGAGAGCT ACAAAACCTG ATCGGTGCCA TCAACCAACT GAACGTTCTT ACCAAAGAAC 
     3061 TTACAAAAAG ACACAACTTA CTGAGAAATG AAGTGCAGAG CCGTGGTGAA GCCTTTGAGA 
     3121 GACGCATCAG TGAGCATGCC TTAGAAACAG AAGATGGCCT AAATAAGACA ATGACTGTTA 
     3181 TAAACAATGC CATCGATTTT GTTCAAGATA ACTATGTGTT AAAGGAGACT TTAAGTGCTA 
     3241 TGACATATAA TCCCAAGGTT TGTGAGTGTA ACCAAAATAT GGACAATATT TTAACATTTG 
     3301 TTTCTGAATT CCAGCATTTG AATGATTCTA TTCAGACTTT GGTCAATAAC AAAGAGAAGT 
     3361 ATAATTTTAT TTTGCAAATT GCTAAAGCCC TTACAGCGAT TCCTAAAGAT GAGAAACTAA 
     3421 ACCAGTTAAA CTTTCAAAAT ATTTATCAAC TATTCAATGA AACCACTTCC CAGGTGAACA 
     3481 AATGTCAGCA AAACATGAGT CATTTAGAAG AAAATATGCT TTCAGTAACA AAGACTGCCA 
     3541 AAGAGTTTGA AACTCGTTTG CAAGGCATTG AATCTAAAGT GACCAAGACA CTCATACCTT 
     3601 ACTATATTTC ATTTAAAAAA GGTGGCATCC TATCAAATGA GAGAGATGTT GATCTTCAGC 
     3661 TGAAGGTACT AAATACCAGA TTTAAGGCAC TGGAAGCAAA GTCCATCCAT CTTTCCGTGA 
     3721 GTTTCTCTTT GCTTAATAAA ACTGTCCGTG AGCTTTCAAT GGCATGCCGT AATGCTTCTA 
     3781 CAGGTACCTG TGGACAGAAT GCACTCATAC CCAGGTGGAC CAAAGGCTCA CTGCCAGGCA 
     3841 GTCAGAGTTC TCAGAAAAGT CTGACAGAAC TTGTGGAATC AATAGTTGAA ATTAAAACTC 
     3901 AAGCCGCCCT ATCTAATTTA ACTTGGAATG TTGACCGATT GTTATCTGAC ACTCTGGCAA 
     3961 ATATTGTCAA GCCTCAAAAG CAGATAAAAT TGCAGAAGAA ACCCAACACA CTTAAGAAAA 
     4021 CGGTGAACAT GACCACCATT CTGATAGGCC GAACACAAAG GAACACAGAC ACCATTATAC 
     4081 ATCCTGTAGC ACAGGAACAT TCCAGCTGCA GCAGCTTCCC GTGCCAGAAT GGTGGCACAT 
     4141 GCATCAGCGG GAGATCCAAC TTCATCTGCG CTTGCCGGCA CCCGTTCATG GGTGACACTT 
     4201 GCACCGTGAA GATCAAGGAA GATGATGCAG TAGCACCAGA TTTTTCCAAA GGATCTTACA 
     4261 GATATGCTCC GATGGTGGCG TTTTTCGTAT CTCACACTCA CGGAATGACG GCACCTGGTC 
     4321 CTATCCTGTT CAATGATCTA AGCGTCAACT ATGGGGCCTC ATATAACCCA AGGACTGGCA 
     4381 AGTTCAGAAT TCCATACCTT GGAGTGTATA TTTTCAAATA CACCATTGAG TCATTCAGTG 
     4441 CTCATATCTC CGGTTTTTTT GTGGTGGATG GAGTAGACAA ACTTAGATTT GAGTCTGAAA 
     4501 ATGCTGACAA TGAGATACAC TGTGACAGAG TTTTGACTGG GGATGCCTTA TTTGAATTAA 
     4561 ATTACGGACA GGAAGTGTGG TTGCGACTTG TAAAAGGAAC AATTCCAATC AAATATCCCC 
     4621 CTGTGACCAC GTTCAGTGGT TACTTGTTAT ATCGCACCAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//