LOCUS       MR224465                8733 bp    DNA     circular  SYN 
DEFINITION  Magel2 (Myc-DDK-tagged) - Mouse melanoma antigen, family L, 2 (Magel2). Note: ORF is codon optimized
ACCESSION   NM_013779
VERSION     NM_013779.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4890..4981 
                     /label="Myc-DDK"  
     misc_feature    5011..5596 
                     /label="PolyA signal"  
     misc_feature    5068..5087 
                     /label="Restriction Enzyme"  
     misc_feature    5872..6544 
                     /label="ColE1"  
     misc_feature    6974..7768 
                     /label="Kan/Neo"  
     misc_feature    7803..8105 
                     /label="SV40 ori"  
     misc_feature    8191..8668 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4872..4878 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4872 
                     /label="Magel2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCAGCTT TCCACCAACC TGGGTGATTC ATCACCTCCG GAATCACCAG 
     1081 TGCCCGCAGT CCACTCCAGG CCCACAGTAC TGATGCGGGC TCCTCCCGCT AGTTCAAGGG 
     1141 CTCCTCCTGT CCCGTGGGAT CCTCCCCCCG TTGATCTCCA GGCCCCTATG GCTGCATGGC 
     1201 AGGCCCCCCA GCCAGCATGG GAAGCTCCGG AGGGTCAGCT GCCTGCGCCT GTCGCCCAGC 
     1261 TGGCTCAGCC TCCAGGGCTG GGCGCACCTA TGGTTCAGGC GCCCCCTCTG GGTGGCGGCA 
     1321 TGGCTAAACC ACCTACTCCA GGAGTCCTGA TGGTGCACCA GCCACCCCCT GGCGCGCCTA 
     1381 TGGCTCAGTC AAGCACGCCC GGCGTGCTGA TGCTGCACCC TTCTGTGACT GGCGCCCCCC 
     1441 TGGCTCACCC CCCCCCACCC GGTACACCCA TGACACACCC ACCCGGGACA TCCATGGCCC 
     1501 ATCCCCCTCC TCCTCCGCCA CCTCCGCCTC CCCCCCCACC TCCGGGCACA CCCATGACTC 
     1561 ACCCACCTCC TCCGGGAACA CCTATGGGAC ACCACCCCCC ACCGGGAAAT CCGATGACCC 
     1621 ACCCCCCTCC TGGCAATCCT ATGGTTCACC CCCTCACCCA TGGCGCGCCT ATGGTGCATG 
     1681 GAGGTCCCCA TGGGACTCCT ATGCCACATG TGCCCATCAC GGGGACCCCT ATTGCTCAGC 
     1741 AACCGACACC CGGTGTCCTG ATGGCGCAGC AGCTTACTCC CGGAGTGCTC ATGGTGCAGC 
     1801 CTCCTGCTCC TGGAGCACCA ATGGTGCAGC CCCCCCCTCA GGCTGCCCTC ATGACCCAAC 
     1861 CAGCCCCAAG TATAACACCA ATGGCAAAGC CTCCTGGACC CGGCGTGGTA ATGATACATC 
     1921 CTCCAGGCGC TCGAGGCCCC ATTATTCAGA CACCCGTATC CGGCGCTCCT ATGGCTCAAA 
     1981 CCGTGCTCCC CCCGGGTCAG CCTTTGGCCA CTTGGGCACC ACAGGGCCAG CCCCTCATCC 
     2041 TGCAGATCCA AAGCCAGGTC ATCCGCGCCC CCCCTCAGGT GCCCAGCGTT CCCCAGGCAC 
     2101 CTCAGGTTCA GCTTGCCACA CCTCCTGGAT GGCAGGCGAC AACGCCAAAT TGGCAGGTCA 
     2161 CCCCCCAGGG ATGGCCGGCT ACTCCTCTGA CGTGGCAGGC AACACAAGTG ACCTGGCAGG 
     2221 CGCCAACCAT TGCATGGCAG GCCACACAGC CAGGCAGGCA GGGGCACTCC ACAATCAGGA 
     2281 CAGGGCATAC ACCCATCCGC CCAGGCCCCG CACCGCTCCT GCGGCAGATC CCGCCTATGA 
     2341 TCAGGCAAAT TCAGCCCGTC ATGAGGCAGG CCCCCCCACT TATTCGCCAG GTGCCAATCC 
     2401 GCCCCGCGCC ACATGGGATC GCCAGCCAGC CTCAGCTGTG GCAGGTGCTC CCTCCACCAC 
     2461 CACCTCTGCG GCAGGCCCCC CAGGCACGGC TTCTCCTTCC ACGAGTGCCC GGTACGGGGC 
     2521 AGGTCAGCAC GGTCCCTCCT GTTGCCCAGA TTCACCTGGT GCCCCAAAGC GGACCACAGG 
     2581 TTCCCCAAAC AGTGCTGCCA GCCCAGCTTA GTATTCCCAT CCCTGTACCC CAAGCAGCCG 
     2641 CCCAGTCCGC CCCCCGCACT GTGCATTGCC CGCCGATCAT TTGGCAAGCT CCCAAAGGTC 
     2701 AGGCTCCCGT CCCTCAGGAA CTTCCCGTGC CACAAGAGCT GCCAGTACCC CAGGAGCTGC 
     2761 CCGTTCCCCA GGAGGTGCCA GTTCCACAGG AGATCCCCGT GCCTCAGGAG ATCCCAGTTC 
     2821 CTCAGGAACT GCCAGTACCC CAAGAACTTC CGGTGCCTCA GGAGCTGCCG GTGCCTCAAG 
     2881 AGCTGCCTGT CCCACAGGAA CTTCCTGTCC CTCAGGAGCT GCCAGTGCCA CAGGAGCTGC 
     2941 CCGTACCACA GGAGCTGCCA GTCCCACTGG AGTTCCAGGA GGTCCAACAG GCTCAGGCAG 
     3001 TCGGATGGCG CGCTCCTAAA GTCCCACCCC ACTTTTGGCA GCCTGTGAGC GCACAAGAGG 
     3061 CCCAGGAACA AGCAACCCAG ATTGCGCACG TGGAACAACA GCAGCCTTTC CAGGGAGCCC 
     3121 CCGCATCCTC TAAAGCGCTG CAAACCCAAC TCCCAACTCA CCAAGCCCAG GCCTCCGGCC 
     3181 TCCAGGCGGA GCTGCCTTCC GTGCAGCTGC AACCCAGCTG GCAGGGTCCT CTGCCCATGT 
     3241 TGCAGGCCCA GCCCGGGGCT TCAGCAACCC TTGCTAACTT TCCTAGGGGC TCCACCAGAA 
     3301 GCAGAATGGC CCCAAGCGGT GAACCGGGTC CATCTTCCCT TGAGCCGCGG GGACCCCCAC 
     3361 GAGAACGGCG AGCACCCGCC CGCGACAAAA AGGGCCCTCC GAAAGAAAGG ATGTTCATCG 
     3421 GAGCGACCTT TTGTGCCCCC AGGGGCGCAA GCGCGTCCCG CGCATACGTG CCAACGGCAT 
     3481 GGAAGAATCT CCCTGCTACC AGCGAAACCT TCCCGGCCAC AAGCAGGGTA TTCCCTTCCA 
     3541 CAAGTCACTT TCAGCCCGCC TCATCTAATG CCTTTCGCGG TCCTAGCGCC GCTAGTGAGT 
     3601 CTCCTAAAAG CTTGCCCTTT GCACTGCAGG ATCCCTACGC CTGTGTGGAA GCCCTCCCGG 
     3661 CGGTGCCGTG GGTCCCTTAT CCCGACGGCA ACGCTAGTTC TGCATGCAAA TCCGTCCCAG 
     3721 CAATACTGAT GGTGGCCGCT GCTGCCCCGC AGGCATCTGC TACGGCCGCC GAAGCCTCTA 
     3781 AGAGCTCTGA GCCCCCTCGG CGGCCCGGCA AGGCTACAAG GAAGAAGAAG CATCTGGAGC 
     3841 CAAAGGAGGA CAACTGTGGA CATAGGCTGA GTAGCAGAGA CTGGAGAGGT CCCCGCACAT 
     3901 GGGGCAATCC TAGTCACAGC GACTGGGAAA TCCAACGCGC AATGCAACTG CTCGGGGACC 
     3961 GCGAGTCATT GTATACCCCT CAGGGCCTCA ACGATTGGGG CTGTCCAAAT ACCAGCCGCA 
     4021 TGCCAAGATC CTTGGAAGGA CCCAGCACCT CCCGGGATCA GGAGTTCTGC GGCGATTCTG 
     4081 GGGGGAGTCA AACATGGATG GCTTCTGAAG TTCCTAGCGT ATCCAGAGGC TCCTCTGCGG 
     4141 CACAAGAAGA TCCTGACCGC GAGAGCCAGC CACTGAGTCC CCTGGATGAA AGAGCTAATG 
     4201 CTCTCGTGCA ATTTCTGTTG GTTAAGGACC AGGCTAAGGT TCCGGTGCAG CTCAGTGAGA 
     4261 TGGTCAATGT GGTCATCCGG GAGTACAAGG ACGACTCACT GGACATCATC AACCGCGCCA 
     4321 ATACGAAACT CGAATGCACA TTCGGGTGTC AACTCAAGGA GGTTGATACC AAAACGCACA 
     4381 CTTACATCAT AGTTAATAAG ATGGCCTACC CACAGTGCAA CCTGTTGGCT TCCTATCTGG 
     4441 AACGCCCTAA ATTTAGCCTC CTGATGGTGG TCCTGTCACT CATTTTCATG AAGGGATATT 
     4501 GTATTCGGGA GAATCTGCTC TTCAGCTTTC TGTTCCAGCT GGGTCTGGAT GTACAAGAGA 
     4561 CAAGCGGACT CTTTCGAATT ACAAAGAAAC TGATTACTAG CGTCTTTGTA CGGCATCGAT 
     4621 ACCTGGAGTA CCGACAGATC CCCTTTACTG AGCCTGCTGA GTACGAGCTT CTCTGGGGCC 
     4681 CCCGAGCGTT TTTGGAGACT AACAGAGTGC ACATTCTGAG ATTTTTGGCG GCGCTTTACG 
     4741 AAAATCAACC TCAAATATGG TCATGTCAGT ACCTGGACAG CCTCGCCGAG TTGGAATACA 
     4801 AGGACGCGAA CGCCGCAGCT GAGGAGAGCC ATGATTCAGA CGACGATGCA CACGACCCCA 
     4861 CATCCTCCCC TCACCCCCAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4921 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4981 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5041 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5101 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5161 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5221 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5281 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5341 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5401 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5461 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5521 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5581 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5641 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5701 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5761 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5821 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5881 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5941 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6001 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6061 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6121 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6181 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6241 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6301 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6361 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6421 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6481 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6541 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6601 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6661 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6721 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6781 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6841 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6901 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6961 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7021 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7081 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7141 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7201 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7261 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7321 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7381 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7441 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7501 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7561 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7621 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7681 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7741 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7801 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7861 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7921 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7981 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8041 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8161 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8221 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8281 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8341 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8401 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8461 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8521 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8581 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8641 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8701 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//