LOCUS       MR224278                8382 bp    DNA     circular  SYN 
DEFINITION  Itgae (Myc-DDK-tagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 1
ACCESSION   NM_008399
VERSION     NM_008399.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4539..4630 
                     /label="Myc-DDK"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    4717..4736 
                     /label="Restriction Enzyme"  
     misc_feature    5521..6193 
                     /label="ColE1"  
     misc_feature    6623..7417 
                     /label="Kan/Neo"  
     misc_feature    7452..7754 
                     /label="SV40 ori"  
     misc_feature    7840..8317 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="Itgae"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTGGCTC TTCCATACCC TTCTCTGCAT GGCCAGCCTG AAGCCCCAGG 
     1081 GAGCCTTCAA TTTGGATGTG GACTGGGCCT GGGTCACAGC ACTCCAGCCA GGTGCACCGG 
     1141 CTGTGCTCAG CTCCCTTCTG CATCAAGACC CCAGCAACAA CCAGACCTGC CTCCTGGTAG 
     1201 CCAGACGCAG CTCCAACAGG AATACAGCCG CCCTGTATCG ATGCGCTATA TCCATCAGTC 
     1261 CAGATGAAAT TGCTTGCCAG CCAGTGGAGC ACATCTGTAT GCCAAAGGGC AGGTACCAGG 
     1321 GAGTGACATT GGTCGGGAAC CACAATGGTG TTTTGGTGTG CATTCAGGTA CAGGCCCGGA 
     1381 AGTTCCGCAG CCTCAACTCA GAGCTCACGG GTGCCTGCAG CCTACTCACC CCCAATTTGG 
     1441 ACCTCCAGGC CCAAGCCTAC TTCTCTGACC TCGAAGGGTT CCTGGATCCA GGAGCACATG 
     1501 TGGACTCTGG TGACTACTGT AGAAGCAAAG GAGGCAGCAC AGGGGAGGAG AAGAAGTCAG 
     1561 CCAGGAGACG CCGGACAGTG GAGGAGGAAG ACGAGGAGGA AGATGGCACT GAGATCGCTA 
     1621 TTGTCCTGGA TGGCTCAGGA AGCATTGAAC CCTCAGACTT CCAGAAGGCC AAAAATTTCA 
     1681 TCTCTACCAT GATGAGGAAC TTCTATGAGA AGTGTTTTGA GTGCAACTTT GCCCTGGTGC 
     1741 AGTATGGGGC CGTGATCCAG ACTGAGTTTG ATCTTCAAGA GAGCCGGGAC ATTAACGCCT 
     1801 CCCTTGCCAA AGTTCAGAGC ATCGTTCAAG TGAAGGAAGT CACTAAGACC GCCTCAGCCA 
     1861 TGCAGCATGT CCTAGACAAT ATCTTCATTC CAAGCCGTGG CTCCAGAAAA AAAGCATTGA 
     1921 AGGTCATGGT GGTACTTACT GATGGTGACA TATTTGGGGA CCCTCTCAAT CTTACGACTG 
     1981 TCATCAACTC CCCAAAGATG CAGGGTGTTG TGCGCTTTGC CATTGGGGTG GGTGATGCAT 
     2041 TCAAGAATAA CAACACTTAC AGAGAACTGA AGCTGATTGC CTCAGACCCC AAAGAGGCCC 
     2101 ACACTTTCAA GGTGACCAAC TACTCAGCTC TGGATGGGCT GCTGAGCAAG CTGCAACAAC 
     2161 ACATCGTTCA TATGGAAGGT ACAGTTGGAG ATGCCCTTCA ATATCAACTC GCACAGACTG 
     2221 GTTTCAGTGC CCAGATCCTG GACAAGGGGC AGGTGTTGCT TGGCACTGTG GGGGCCTTTA 
     2281 ACTGGTCTGG GGGTGCGCTG CTCTACAGCA CACAGAATGG CCGGGGCTGC TTCCTAAACC 
     2341 AGACAGCCAA GGAGGACTCC AGGACTGTAC AGTACAGCTA CCTAGGTTAC TCCCTTGCGG 
     2401 TGCTACACAA GGCCCACGGC GTCTCTTACG TGGCAGGGGC TCCAAGGCAC AAGCTCCGTG 
     2461 GGGCTGTGTT TGAGCTCCGG AAGGAGGACA GAGAGGAGGA TGCCTTTGTG CGACGGATAG 
     2521 AAGGAGAGCA GATGGGGTCC TACTTTGGCT CTGTGCTGTG CCCTGTGGAT ATCGACATGG 
     2581 ATGGGACCAC AGATTTTCTA CTGGTGGCTG CTCCATTTTA CCACATCCGT GGAGAAGAAG 
     2641 GCAGAGTCTA TGTGTACCAG GTGCCTGAGC AGGATGCTTC CTTCTCCTTG GCACACACAC 
     2701 TGAGTGGGCA CCCTGGGCTC ACCAATAGCC GCTTTGGCTT TGCCATGGCA GCTGTGGGGG 
     2761 ATATCAACCA AGACAAATTC ACAGATGTGG CCATTGGGGC CCCTCTGGAG GGTTTTGGGG 
     2821 CAGGTGACGG GGCCAGCTAT GGCAGCGTGT ACATCTACAA CGGACACTCA GGTGGCTTGT 
     2881 ATGACAGTCC TTCCCAGCAG ATCCGAGCCT CCTCTGTGGC CTCGGGACTC CACTACTTCG 
     2941 GCATGTCTGT GTCCGGTGGG TTAGATTTCA ATGGCGATGG CCTTGCCGAT ATCACAGTTG 
     3001 GCTCTCGGGA CTCGGCGGTT GTGCTCCGCT CACGCCCTGT GGTTGACCTG ACCGTATCTA 
     3061 TGACCTTTAC CCCCGATGCA CTGCCCATGG TCTTCATAGG CAAGATGGAT GTAAAACTGT 
     3121 GTTTTGAAGT TGACTCTTCA GGCGTAGCTT CTGAGCCAGG CCTTAGAGAG ATGTTCCTCA 
     3181 ACTTTACGGT GGATGTGGAT GTGACCAAGC AAAGGCAGAG GCTGCAGTGT GAGGACTCGA 
     3241 GCGGTTGTCA GAGCTGCCTC AGGAAGTGGA ACGGAGGGTC CTTTCTGTGT GAACACTTCT 
     3301 GGCTCATCTC CACAGAGGAG CTTTGTGAGG AGGACTGCTT CTCCAACATC ACCATCAAGG 
     3361 TCACCTACGA GTTCCAGACG TCTGGGGGAA GGAGGGACTA CCCCAACCCC ACCCTGGACC 
     3421 ACTACAAGGA ACCCTCTGCC ATCTTCCAGC TGCCCTACGA GAAGGACTGC AAGAACAAAG 
     3481 TGTTCTGCAT CGCTGAGATC CAGCTGACCA CCAACATCTC CCAGCAGGAA CTGGTGGTGG 
     3541 GCGTCACAAA GGAGGTGACC ATGAACATCA GCCTGACTAA CTCTGGAGAG GATTCCTACA 
     3601 TGACAAACAT GGCTCTCAAT TATCCCAGAA ACTTACAGTT TAAGAAGATA CAAAAGCCAG 
     3661 TATCTCCAGA TGTTCAGTGT GATGACCCCA AGCCAGTTGC TTCTGTCCTG GTCATGAACT 
     3721 GCAAGATTGG TCACCCCATA CTCAAGAGAT CATCTGTGAA TGTCTCAGTA ACTTGGCAGC 
     3781 TAGAGGAGAG TGTCTTTCCA AATAGGACAG CAGATATCAC TGTGACCATC TCCAATTCCA 
     3841 ATGAGAAGTC TTTGGCCAGA GAGACACGCA GCCTTCAATT CAGACATGCC TTCATTGCAG 
     3901 TTCTTTCCAG ACCCTCAGTG ATGTACATGA ACACAAGCCA AAGCCCTTCT GACCACAAAG 
     3961 AATTCTTCTT CAATGTGCAT GGGGAGAATC TTTTTGGAGC TGTATTCCAG TTGCAAATTT 
     4021 GTGTCCCCAT CAAATTACAA GATTTCCAGA TTGTAAGAGT GAAGAATCTG ACAAAGACTC 
     4081 AGGACCACAC TGAGTGCACC CAGAGTCAAG AGCCTGCGTG TGGAAGCGAT CCTGTGCAGC 
     4141 ATGTAAAAGA ATGGCATTCA GTGGTCTGTG CTATCACATC TAATAAAGAA AATGTAACCG 
     4201 TGGCCGCAGA GATCTCCGTG GGCCACACTA AGCAGTTACT AAGAGATGTA TCTGAACTGC 
     4261 CGATCCTTGG TGAAATATCT TTCAACAAAT CTCTCTATGA GGGGCTGAAT GCAGAGAACC 
     4321 ACAGGACGAA GATCACTGTC ATCTTCCTGA AAGAGGAGGA GACACGCTCT TTGCCTCTCA 
     4381 TCATTGGCAG CAGCATCGGA GGCCTCCTGG TCTTGGTTGT GATTATAGCC ATTTTGTTCA 
     4441 AGTGTGGCTT TTTCAAAAGA AAATATCAAC AACTGAACTT GGAGAGCACA AGGAGGGCCC 
     4501 AGCTGAAGGC TGACAGTCTG CTCCAAGATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4561 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5341 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5401 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5461 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5521 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5581 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5641 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5701 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5761 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5821 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5881 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5941 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6001 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6061 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6121 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6181 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6241 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6301 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6361 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6421 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6481 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6541 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6601 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6661 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6721 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6781 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6841 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6901 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6961 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7021 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7081 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7141 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7201 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7261 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7321 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7381 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7441 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7501 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7561 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7621 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7801 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7861 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7921 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7981 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8041 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8101 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8161 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8221 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8281 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8341 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//