LOCUS       MR224235                7044 bp    DNA     circular  SYN 
DEFINITION  Lrrc33 (Myc-DDK-tagged) - Mouse leucine rich repeat containing 33 (Lrrc33)
ACCESSION   NM_146069
VERSION     NM_146069.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3201..3292 
                     /label="Myc-DDK"  
     misc_feature    3322..3907 
                     /label="PolyA signal"  
     misc_feature    3379..3398 
                     /label="Restriction Enzyme"  
     misc_feature    4183..4855 
                     /label="ColE1"  
     misc_feature    5285..6079 
                     /label="Kan/Neo"  
     misc_feature    6114..6416 
                     /label="SV40 ori"  
     misc_feature    6502..6979 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3183..3189 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3183 
                     /label="Nrros"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGAGCCT TTGGAGACTG GAAGCATTGA ATCCTCTGGA ACTGGCAATG 
     1081 TTGTTGTGAG CCACCAGAGA GCTGTGCCTG AGATGGAGTT CCCGCCCCTC TGGCTTTGCC 
     1141 TGGGTTTTCA CTTCTTGATT GTGGAATGGA GGAGCGGACC TGGGACAGCC ACTGCAGCTT 
     1201 CCCAAGGAGG CTGCAAGGTG GTCGACGGAG TCGCTGACTG CCGGGGTCTG AACCTTGCTT 
     1261 CGGTACCCAG CAGCCTCCCA CCCCATTCCC GGATGCTCAT TCTGGATGCT AACCCTCTCA 
     1321 AGGACCTGTG GAACCATTCC CTCCAGGCCT ACCCACGCCT GGAGAACCTC AGCCTGCATA 
     1381 GCTGTCACCT GGACCGTATC AGCCACTACG CCTTCCGAGA GCAAGGCCAC TTACGCAACC 
     1441 TGGTTCTGGC AGACAACCGC CTCTCGGAGA ACTACAAGGA GTCAGCCGCA GCACTCCACA 
     1501 CCCTGCTGGG ACTTCGGAGA CTGGACTTGT CTGGAAACTC CCTGACCGAA GACATGGCGG 
     1561 CTCTCATGCT TCAGAACCTC TCCTCGTTGG AGGTTGTGTC CTTGGCAAGG AATACCCTCA 
     1621 TGAGGCTCGA CGACTCTATC TTTGAGGGCC TGGAACACCT TGTAGAGCTA GATTTGCAGA 
     1681 GAAACTACAT CTTTGAGATC GAGGGCGGGG CGTTTGACGG CTTGACTGAG CTGAGGCGTC 
     1741 TCAACCTTGC CTATAACAAC CTCCCCTGCA TTGTGGACTT CAGCCTCACC CAGTTACGGT 
     1801 TCCTCAACGT CAGTTATAAC ATCCTAGAGT GGTTCCTGGC AGCCAGGGAG GAGGTGGCCT 
     1861 TCGAATTGGA GATACTGGAC CTGTCTCACA ACCAGCTGCT CTTCTTCCCA CTCTTGCCTC 
     1921 AGTGCGGCAA GCTGCACACC CTCCTGCTCC AGGACAACAA CATGGGCTTC TACAGGGAGC 
     1981 TTTACAACAC CTCCTCCCCG CAGGAGATGG TGGCCCAGTT CCTTCTTGTG GACGGCAATG 
     2041 TGACTAACAT CACAACTGTC AACCTCTGGG AGGAGTTTTC CTCCAGCGAC CTGTCAGCTC 
     2101 TTCGCTTCCT GGACATGAGC CAAAACCAGT TCCGGCACTT GCCGGATGGT TTCCTGAAGA 
     2161 AGACACCTTC CCTTTCCCAC CTGAACCTCA ACCAGAACTG CCTGAAGATG CTCCACATCC 
     2221 GGGAGCACGA GCCGCCGGGA GCACTCACCG AGCTGGATCT GAGCCACAAC CAGTTGGCAG 
     2281 AGCTGCACCT GGCTCCGGGG CTCACGGGCT CCCTCAGGAA CCTCCGGGTG TTCAACCTGA 
     2341 GTTCCAACCA GCTCCTGGGG GTCCCCACTG GCCTTTTCGA CAATGCCAGC AGCATCACTA 
     2401 CAATCGACAT GAGTCACAAT CAGATCTCAC TTTGTCCTCA GATGGTGCCC GTAGACTGGG 
     2461 AGGGGCCTCC CAGTTGTGTG GATTTCAGAA ACATGGGCTC TTTGAGGAGC CTTTCTCTGG 
     2521 ACGGCTGTGG GCTGAAGGCA TTACAAGACT GCCCGTTCCA GGGGACCTCC CTCACTCATT 
     2581 TAGACCTGTC TAGCAACTGG GGGGTTCTGA ATGGGAGCAT CAGCCCTCTC TGGGCTGTTG 
     2641 CCCCGACATT ACAGGTCCTG TCTCTCAGGG ACGTGGGCCT TGGTTCTGGC GCTGCAGAGA 
     2701 TGGACTTCTC TGCGTTTGGG AATCTGCGGG CGTTGGATCT GTCGGGAAAC TCCCTGACCA 
     2761 GCTTCCCAAA GTTCAAGGGC AGTTTGGCCC TTCGGACTCT CGACCTCCGC AGAAACTCTC 
     2821 TCACGGCCCT CCCTCAGAGG GTTGTGTCCG AGCAGCCTCT GAGGGGTCTG CAGACCATCT 
     2881 ACCTCAGCCA GAACCCTTAT GACTGCTGTG GGGTGGAAGG ATGGGGGGCC CTGCAGCAGC 
     2941 ACTTCAAGAC TGTTGCGGAC TTGTCCATGG TCACTTGCAA CCTCTCTTCC AAGATCGTCC 
     3001 GTGTGGTGGA GCTGCCCGAA GGCCTGCCTC AGGGCTGTAA GTGGGAACAG GTGGACACTG 
     3061 GTCTCTTCTA CCTCGTGCTC ATCCTGCCCA GCTGCCTCAC CCTGCTGGTG GCCTGTACTG 
     3121 TCGTCTTCCT CACTTTTAAG AAGCCTTTGC TTCAGGTCAT CAAGAGCCGC TGCCACTGGT 
     3181 CCTCCATATA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3241 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3301 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3361 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3421 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3481 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3541 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3601 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3661 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3721 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3781 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3841 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3901 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3961 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4021 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4081 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4741 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4801 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4861 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4921 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4981 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5041 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5101 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5161 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5221 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5281 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5341 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5401 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5461 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5521 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5581 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5641 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5701 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5761 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5821 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5881 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5941 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6001 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6061 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6121 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6181 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6241 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6301 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6361 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6421 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6481 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6541 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6601 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6661 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6721 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6781 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6841 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6901 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6961 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7021 TAACAAAAAT TTAACGCGAA TTTT
//