LOCUS       MR224145                7575 bp    DNA     circular  SYN 
DEFINITION  Taok3 (Myc-DDK-tagged) - Mouse TAO kinase 3 (Taok3), transcript variant 3
ACCESSION   NM_001081308
VERSION     NM_001081308.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3732..3823 
                     /label="Myc-DDK"  
     misc_feature    3853..4438 
                     /label="PolyA signal"  
     misc_feature    3910..3929 
                     /label="Restriction Enzyme"  
     misc_feature    4714..5386 
                     /label="ColE1"  
     misc_feature    5816..6610 
                     /label="Kan/Neo"  
     misc_feature    6645..6947 
                     /label="SV40 ori"  
     misc_feature    7033..7510 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3714..3720 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3714 
                     /label="Taok3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGTAAAGGG GCACTGAAGG ACCCAGAGAT TGCTGATCTG TTCTTCAAGG 
     1081 ATGACCCCGA AGAGCTTTTT ATCGATTTGC ATGAAATTGG ACATGGGAGC TTTGGAGCCG 
     1141 TGTACTTTGC GACAAATGCG CACACCAACG AGGTGGTCGC TGTTAAGAAA ATGTCCTACA 
     1201 GTGGGAAGCA GACCCATGAG AAATGGCAAG ATATCCTTAA GGAAGTTAAG TTTCTGCAGC 
     1261 AGCTGAAGCA TCCTAACACC ATCGAGTACA AAGGCTGTTA TTTAAAGGAG CACACTGCAT 
     1321 GGTTGGTGAT GGAGTACTGC CTAGGTTCAG CCTCTGACTT GTTAGAAGTT CATAAGAAAC 
     1381 CACTTCAGGA AGTGGAGATC GCTGCTATCA CCCATGGTGC CTTGCAGGGG CTGGCCTACC 
     1441 TCCACTTTCA CTCCCTGATT CACAGGGACA TTAAAGCAGG GAACATCCTC CTCACAGAGC 
     1501 CAGGTCAGGT GAAGCTGGCT GACTTCGGAT CTGCCTCCAT GGCTTCTCCC GCCAACTCCT 
     1561 TTGTGGGGAC TCCTTACTGG ATGGCCCCAG AGGTAATCTT AGCTATGGAC GAAGGGCAGT 
     1621 ACGACGGGAA AGTTGACATC TGGTCGCTTG GCATCACCTG CATAGAGTTG GCGGAGCGGA 
     1681 AGCCCCCCCT CTTCAACATG AACGCCATGA GCGCCCTCTA CCACATCGCT CAGAATGACT 
     1741 CCCCCACGCT ACAGTCCAGG GAATGGACAG ACTCCTTCAG GAGATTCGTT GATTACTGCT 
     1801 TGCACAAAAT ACCTCAGGAA AGACCAGCCG CGGTGGAGCT GTTGCGGCAT GACTTCATTC 
     1861 GGAGGGAGCG GCCACCGAAG GTCCTCATTG ATCTCATACA GAGGACAAAA GATGCTGTCC 
     1921 GAGAGCTGGA CAACCTGCAG TACAGGAAAA TGAAGAAGAT CCTCTTCCAG GAGACTCGGA 
     1981 ACGGACCCTT GAATGAGTCG CAAGAAGAAG AAGAAGATGG TGAGCAAGGA AGCAACCTGA 
     2041 ACCGAGAGGT GGACAGTCTG GGCAGCATCC ACTCCATTCC CAGCACATCA GTGAGCACAG 
     2101 GCAGCCGGAG CAGCAGTGTG AACAGCATGC AGGAGGTCAT GGATGAGAGC AGCTCTGAGC 
     2161 TCGTCATGAT GCAGGAGGAT GAAGGCACAG CCAACTCCAG CGCCTCCACG GTACACAAGA 
     2221 AGGATCATGT ATTCGTAAGG GATGAGGCGG GCCACGGCGA TCCCAGGCCT GAGCCGCGGC 
     2281 CTACCCAGTC AGTTCAGAGC CGGGCCCTCC ACTATCGGAA CCGAGAGCGC TTTGCCACGA 
     2341 TCAAATCCGC GTCTTTGGTT ACACGGCAGA TCCATGAGCA TGAGCAGGAG AACGAGCTGC 
     2401 GGGAACAGAT GTCAGGTTAT AAGCGGATGC GGCGCCAGCA CCAGAAGCAG CTGATCGCCC 
     2461 TGGAGAACAA GCTGAAGGCT GAGATGGATG AGCACCGCCT CAAGCTCCAG AAGGAGGTGG 
     2521 AGACGCACGC CAACAACTCG TCCATCGAGC TGGAGAAGCT GGCCAAGAAG CAAGTGGCTA 
     2581 CCATAGAAAA GGAGGCAAAG GTAGCCGCAG CGGATGAGAA GAAGTTCCAG CAGCAGATCC 
     2641 TGGCCCAGCA GAAGAAGGAC TTGACAACTT TCTTAGAAAG TCAGAAGAAG CAGTACAAGA 
     2701 TTTGTAAGGA GAAAATAAAA GAGGAAATGA ACGAGGACCA TAGCACACCC AAGAAGGAGA 
     2761 AGCAGGAGCG GATATCGAAG CATAAAGAAA ACCTGCAGCA CACGCAGGCC GAGGAGGAAG 
     2821 CCCACCTGCT CACTCAACAG AGGCTGTACT ACGACAGAAA CTGCCGCTTC TTCAAACGGA 
     2881 AAATAATGAT CAAGCGCCAC GAGGTGGAAC AGCAGAACAT TCGGGAGGAA TTAAATAAGA 
     2941 AGAGGACCCA GAAGGAAATG GAGCACGCCA TGCTGATCCG GCATGATGAA TCCACCCGAG 
     3001 AGCTGGAGTA CAGGCAGCTG CACACCCTGC AGAAGCTCCG CATGGATCTG ATCCGGCTGC 
     3061 AGCACCAGAC GGAACTGGAG AACCAGCTGG AGTACAATAA GAGGCGGGAG CGGGAGCTGC 
     3121 ACCGGAAGCA CGTCATGGAG CTTCGGCAAC AGCCGAAAAA CTTAAAGGCC ATGGAAATGC 
     3181 AAATTAAAAA ACAGTTTCAG GACACTTGCA AAGTACAGAC CAAACAGTAC AAAGCCCTCA 
     3241 AAAATCACCA GTTGGAAGTC ACTCCAAAGA ATGAGCACAA AGCAATCCTA AAGACACTGA 
     3301 AGGACGAGCA GACGAGGAAG CTGGCCATTT TGGCCGAGCA GTATGAACAG AGTATAAACG 
     3361 AGATGATGGC CTCTCAAGCG TTACGGCTAG ACGAGGCACA AGAGGCAGAG TGCCAGGCCT 
     3421 TGAGACTACA GCTCCAGCAG GAGATGGAGC TGCTCAACGC CTACCAGAGC AAAATCAAGA 
     3481 TGCAAACGGA GGCCCAGCAC GAGCGCGAGC TACAGAAGCT AGAGCAGAGG GTGTCCCTGC 
     3541 GCAGAGCGCA TCTTGAGCAG AAGATTGAAG AGGAGCTGGC TGCCCTGCAG AAGGAACGCA 
     3601 GTGAGAGGAT CAAGACTCTC CTGGAGAGAC AAGAGCGAGA GACCGAGACT TTTGACATGG 
     3661 AGAGCCTCAG AATGGGCTTT GGGAATTTGG TGACATTAGA TTTTCCTAAG GAGGACTATA 
     3721 GAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3781 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3841 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3901 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3961 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4021 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4081 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4141 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4201 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4261 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4321 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4381 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4441 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4501 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4561 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4621 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4681 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4741 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4801 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4861 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4921 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4981 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5041 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5101 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5161 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5221 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5281 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5341 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5401 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5461 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5521 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5581 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5641 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5701 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5761 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5821 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5881 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5941 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6001 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6061 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6121 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6181 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6241 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6301 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6361 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6421 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6481 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6541 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6601 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6661 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6721 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6781 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6841 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6901 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6961 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7021 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7081 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7141 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7201 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7261 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7321 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7381 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7441 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7501 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7561 TTTAACGCGA ATTTT
//