LOCUS       MR223981                9405 bp    DNA     circular  SYN 
DEFINITION  Robo2 (Myc-DDK-tagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2)
ACCESSION   NM_175549
VERSION     NM_175549.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5562..5653 
                     /label="Myc-DDK"  
     misc_feature    5683..6268 
                     /label="PolyA signal"  
     misc_feature    5740..5759 
                     /label="Restriction Enzyme"  
     misc_feature    6544..7216 
                     /label="ColE1"  
     misc_feature    7646..8440 
                     /label="Kan/Neo"  
     misc_feature    8475..8777 
                     /label="SV40 ori"  
     misc_feature    8863..9340 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5544..5550 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5544 
                     /label="Robo2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCCTCTG ATGTTTACAC TATTATTGCT CTTTGGATTT CTCTGCATTC 
     1081 AGATTGATGG ATCTCGTCTT CGTCAAGAAG ACTTTCCCCC CAGGATCGTA GAACACCCTT 
     1141 CTGATGTCAT CGTCTCCAAG GGAGAGCCCA CCACTCTGAA CTGTAAAGCA GAGGGCCGAC 
     1201 CCACCCCCAC CATTGAATGG TACAAGGATG GTGAGAGGGT GGAGACAGAC AAGGATGATC 
     1261 CCAGGTCCCA CAGAATGCTT CTGCCCAGCG GATCTTTATT CTTTTTGCGA ATTGTTCATG 
     1321 GGCGCAGAAG TAAACCGGAC GAAGGGAGTT ACGTTTGTGT TGCAAGGAAC TATCTTGGTG 
     1381 AAGCAGTGAG TCGAAATGCA TCTCTGGAAG TGGCATTATT GCGAGATGAC TTCCGGCAAA 
     1441 ACCCCACAGA TGTGGTAGTC GCAGCTGGAG AGCCTGCAAT CTTGGAGTGC CAGCCACCAC 
     1501 GGGGACACCC AGAACCAACC ATCTACTGGA AAAAGGACAA AGTCCGAATT GATGACAAGG 
     1561 AAGAGAGAAT AAGTATCCGT GGTGGGAAGC TGATGATCTC TAATACTAGG AAAAGCGATG 
     1621 CTGGCATGTA CACCTGTGTG GGAACCAATA TGGTGGGAGA AAGGGACAGC GACCCTGCAG 
     1681 AGCTCACTGT CTTTGAACGA CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTGCTAG 
     1741 AGGAAGAAGC TGTAGAATTC CGTTGTCAGG TCCAAGGAGA TCCCCAGCCA ACGGTGAGGT 
     1801 GGAAAAAAGA TGATGCAGAC TTGCCGAGAG GAAGGTATGA TATCAAAGAT GACTACACGC 
     1861 TGAGAATTAA AAAGGCCATG AGTACTGATG AAGGTACCTA TGTGTGTATT GCTGAGAATC 
     1921 GGGTGGGAAA AGTGGAAGCC TCTGCTACCC TCACTGTCCG AGCTCCTCCA CAGTTTGTGG 
     1981 TTAGGCCAAG AGATCAGATC GTTGCTCAAG GCCGAACAGT GACATTCCCC TGTGAAACTA 
     2041 AAGGAAACCC ACAGCCAGCT GTTTTTTGGC AGAAAGAAGG CAGCCAGAAC CTACTTTTCC 
     2101 CGAATCAACC TCAGCAGCCC AACAGCCGAT GTTCAGTGTC GCCCACGGGG GACCTCACCA 
     2161 TCACCAACAT CCAGCGTTCA GATGCGGGTT ACTACATCTG CCAGGCCCTA ACCGTGGCAG 
     2221 GAAGCATTTT AGCTAAAGCA CAGTTGGAAG TTACTGACGT TTTGACAGAT AGACCTCCAC 
     2281 CCATAATCTT GCAAGGACCA ATAAACCAAA CACTTGCAGT AGACGGTACA GCATTGTTGA 
     2341 AGTGTAAAGC CACTGGTGAG CCTCTGCCTG TAATTAGCTG GCTAAAGGAG GGCTTTACTT 
     2401 TTCTGGGGAG AGATCCAAGA GCCACGATCC AAGACCAAGG AACACTGCAG ATTAAGAATT 
     2461 TACGGATATC TGATACTGGC ACTTATACTT GTGTGGCTAC AAGTTCCAGT GGAGAGACTT 
     2521 CCTGGAGTGC AGTGCTGGAT GTAACAGAAT CTGGAGCAAC AATCAGTAAA AATTATGATA 
     2581 TGAATGACCT CCCGGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTTCT AAGAACAGTG 
     2641 TCACCTTATC CTGGCAGCCA GGTACACCTG GCGTTCTTCC TGCAAGCGCG TATATCATTG 
     2701 AGGCTTTCAG CCAATCGGTG AGCAATAGCT GGCAGACAGT GGCAAACCAT GTTAAGACAA 
     2761 CTCTGTATAC AGTAAGGGGG CTGAGGCCCA ACACAATCTA CTTGTTTATG GTCAGAGCGA 
     2821 TCAACCCACA AGGTCTCAGT GATCCAAGTC CTATGTCGGA TCCTGTACGC ACACAAGATA 
     2881 TCAGCCCCCC AGCACAAGGA GTGGACCACA GACAGGTGCA GAAGGAATTA GGTGATGTCG 
     2941 TTGTTCGTCT CCATAATCCA GTTGTCCTGA CACCTACAAC TGTTCAAGTC ACATGGACGG 
     3001 TGGACCGACA ACCCCAGTTT ATTCAGGGCT ACAGAGTGAT GTACCGTCAG ACTTCGGGAC 
     3061 TACAAGCCTC AACTGTGTGG CAGAATCTAG ACGCCAAAGT CCCGACTGAG AGGAGTGCTG 
     3121 TCCTTGTGAA TTTGAAAAAG GGGGTGACTT ATGAAATTAA AGTCCGGCCG TATTTTAACG 
     3181 AGTTCCAAGG AATGGACAGT GAATCGAAAA CAGTCCGAAC CACTGAGGAA GCCCCAAGTG 
     3241 CCCCTCCCCA GTCTGTCACT GTGCTGACAG TTGGAAGTCA CAACAGCACA AGCATCAGTG 
     3301 TTTCCTGGGA TCCTCCACCA GCCGACCACC AGAATGGAAT TATTCAGGAA TATAAGATCT 
     3361 GGTGTCTGGG AAACGAAACG CGATTCCATA TCAATAAAAC GGTGGATGCA GCCATTCGCT 
     3421 CTGTAGTAAT AGGTGGCTTG TTCCCTGGAA TTCAGTACCG GGTAGAAGTG GCAGCTAGCA 
     3481 CAAGTGCAGG GGTTGGAGTA AAAAGTGAAC CACAGCCGAT AATAATTGGG GGACGTAACG 
     3541 AAGTTGTCAT TACTGAAAAC AATAACAGCA TCACTGAGCA AATCACGGAT GTCGTGAAGC 
     3601 AACCGGCATT TATAGCTGGC ATTGGTGGTG CCTGCTGGGT AATTCTGATG GGTTTTAGCA 
     3661 TCTGGTTGTA CTGGAGAAGA AAGAAGAGAA AGGGACTCAG CAATTATGCT GTAACATTTC 
     3721 AAAGAGGAGA TGGAGGACTA ATGAGCAATG GGAGCCGTCC AGGTCTTCTA AATGCTGGCG 
     3781 ATCCCAATTA CCCATGGCTT GCTGATTCTT GGCCAGCCAC GAGTTTGCCA GTGAACAATA 
     3841 GCAATAGTGG CCCAAATGAA ATTGGAAATT TTGGGCGTGG AGATGTGCTG CCTCCGGTGC 
     3901 CAGGCCAAGG GGATAAAACA GCGACCATGC TCTCGGATGG AGCCATTTAT AGCAGCATTG 
     3961 ACTTCACTAC CAAAACCACT TACAACAGTT CCAGCCAAAT AACACAGGCC ACCCCATATG 
     4021 CCACTACACA AATCCTGCAT TCAAACAGCA TCCACGAACT GGCAGTTGAT CTTCCTGATC 
     4081 CACAGTGGAA AAGCTCAGTT CAACAGAAGA CAGACCTCAT GGGATTTGGT TATTCGCTAC 
     4141 CTGATCAGAA CAAGGGGAAC AACGCCTTAC TTTACATCCC TGACTACCGA TTGGCTGAGG 
     4201 GATTGTCTAA TAGAATGCCA CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA 
     4261 ACAGCTCAGA AAGGAGTGGC AGTCTCTCAG GTGGGAAAGG TGGAAAAAAG AAGAAAACTA 
     4321 AAAATTCTTC GAAAGCGCAG AAAAACAACG GATCCACTTG GGCTAATGTC CCTCTACCTC 
     4381 CTCCTCCCGT CCAGCCCCTT CCTGGTACAG AGCTGGGCCA CTATGCTGCG GAACAAGAAA 
     4441 ATGGCTATGA CAGTGACAGC TGGTGCCCAC CATTACCGGT GCAAACATAC TTGCATCAGG 
     4501 GTATGGAAGA TGAGCTGGAA GAAGACGAAG ATCGGGTCCC AACACCTCCT GTTCGCGGCG 
     4561 TGGCCTCTTC ACCAGCTATC TCTTTTGGAC AGCAGTCCAC TGCCACTCTT ACTCCATCCC 
     4621 CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACTTGGA TGAGTTGACA AGGGCCTATC 
     4681 AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TACCCCACCT CCACAACCCC 
     4741 CAGCTCCGCC ATTAGGTTAC GTGTCCGGAG CCTTGATTTC TGATTTGGAG ACAGATGTTC 
     4801 CAGATGAGGA TGCTGATGAT GAAGAGGAAC CATTAGAAAT TCCCAGGCCC CTCAGAGCAC 
     4861 TAGACCAGAC ACCTGGATCC AGTATGGACA ATCTAGACAG CTCTGTCACA GGAAAAGCCT 
     4921 TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT AACACCAGTG 
     4981 CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA GGACGGATGG 
     5041 ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT CCCCCACCAC 
     5101 CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA CACTCTGGTA 
     5161 ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA GCAGCCAACT 
     5221 TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG TGGCAGCGTC 
     5281 AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA GCCCCACACA 
     5341 AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC 
     5401 CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG 
     5461 CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG 
     5521 TGGGCTCAAA TAGTCAAGGA CAGTTTACAG AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5581 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5641 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5701 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5761 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5821 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5881 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5941 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     6001 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     6061 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6121 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6181 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6241 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6301 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6361 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6421 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6481 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6541 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6601 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6661 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6721 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6781 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6841 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6901 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6961 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     7021 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     7081 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7141 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7201 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7261 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7321 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7381 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7441 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7501 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7561 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7621 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7681 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7741 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7801 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7861 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7921 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7981 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     8041 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     8101 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8161 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8221 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8281 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8341 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8401 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8461 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8521 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8581 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8641 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8701 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8761 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8821 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8881 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8941 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     9001 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9061 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9121 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9181 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9241 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9301 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9361 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//