LOCUS       MR223974                8580 bp    DNA     circular  SYN 
DEFINITION  Slk (Myc-DDK-tagged) - Mouse STE20-like kinase (yeast) (Slk), transcript variant 1
ACCESSION   NM_009289
VERSION     NM_009289.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4737..4828 
                     /label="Myc-DDK"  
     misc_feature    4858..5443 
                     /label="PolyA signal"  
     misc_feature    4915..4934 
                     /label="Restriction Enzyme"  
     misc_feature    5719..6391 
                     /label="ColE1"  
     misc_feature    6821..7615 
                     /label="Kan/Neo"  
     misc_feature    7650..7952 
                     /label="SV40 ori"  
     misc_feature    8038..8515 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4719..4725 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4719 
                     /label="Slk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTTCTTC AATTTCCGTA AGATCTTCAA GTTGGGGAGC GAGAAGAAGA 
     1081 AGAAACAGTA CGAACACGTG AAGAGAGACC TGAACCCCGA AGAGTTTTGG GAGATTATTG 
     1141 GAGAACTGGG CGACGGAGCC TTCGGGAAAG TCTATAAGGC CCAGAATAAA GAGACCAATG 
     1201 TTTTAGCTGC TGCAAAGGTG ATTGACACCA AATCTGAAGA AGAGCTTGAA GATTATATGG 
     1261 TTGAGATTGA CATATTAGCA TCTTGTGATC ACCCAAACAT CGTCAAGCTT CTAGATGCCT 
     1321 TCTATTACGA GAACAACCTT TGGATCCTCA TTGAATTCTG TGCAGGGGGA GCAGTGGATG 
     1381 CTGTGATGCT TGAACTTGAG AGACCATTAA CTGAATCCCA AATCCAAGTA GTCTGCAAGC 
     1441 AGACATTAGA GGCATTGAAT TACTTACATG ACAATAAAAT CATCCACCGA GATCTAAAAG 
     1501 CTGGCAATAT TCTCTTTACC TTAGATGGAG ACATTAAATT AGCGGATTTT GGAGTATCAG 
     1561 CTAAAAATAC CAGGACAATT CAAAGGAGGG ATTCATTTAT TGGCACACCA TATTGGATGG 
     1621 CTCCTGAAGT AGTCATGTGT GAGACATCAA AGGACAGACC TTATGACTAC AAAGCTGATG 
     1681 TTTGGTCCCT GGGTATTACT TTAATAGAAA TGGCTGAGAT AGAGCCACCT CATCATGAGT 
     1741 TAAATCCAAT GCGCGTGCTG CTGAAAATTG CAAAATCTGA GCCCCCAACA TTAGCACAGC 
     1801 CATCAAAATG GTCTTCAAAT TTTAAGGACT TTCTAAGGAA ATGCTTGGAA AAGAATGTGG 
     1861 ATGCGCGGTG GACCACGTCT CAGCTGTTGC AGCATCCCTT TGTTACCGTT GATTCCAACA 
     1921 AACCAGTCCG AGAGTTGATT GCTGAGGCAA AGGCTGAAGT AACAGAAGAA GTTGAAGATG 
     1981 GCAAGGAAGA AGATGAGGAG GAGGAAGCAG AGAATGCTCT GCCAATACCT GCAAATAAAC 
     2041 GTGCCTCCTC TGACCTCAGC ATTGCCAGCT CTGAAGAAGA TAAACTTTCA CAAAATGCTT 
     2101 GTATTTTGGA ATCTGTGTCA GAAAGAACAG AACAAAGTAC TTCTGAGGAT AAATTTAGCA 
     2161 ACAAAATTCT TAATGAGAAA CCTACGACTG ACGGTCCTGA GAAGGCTGTG GATGAGCATG 
     2221 CAAGTGATGT GAACTTAGAA ACTGGGGCTG AACTAAATGA CCAAACAGTA GGAATCCATG 
     2281 AGAATGGGAG AGAGAAGAAA AGACCCAAGC TGGAAAATCT GCCAGATACA CAAGACCAGC 
     2341 AAACTGTGGA TGTTAATTCA GTCAGTGAAG AAAATGAGAA TAATAGAGTA ACTTTAGAAA 
     2401 CGAACACTGA TTGTCTGAAA CCAGAGGAAG ACAGAAATAA AGAAAACCAA GAGACACTTG 
     2461 AGAGTAAACT TATACAATCT GAAGAAATTA ATGACACACA TATTCAAACA ATGGACTTAG 
     2521 TTTCTCAAGA GACTGGAGAA AAAGAAGCAG ATTTTCAGGC AGTTGACAAT GAAGTTGGGC 
     2581 TTACAAAGGA AGAAACCCAA GAGAAATTAG GAAAAGATGG TACAGCTCAA AAAGTTATAA 
     2641 CCAGTGATAG AAGCAGTGAG GTGGGGACAG ACGAGGCTCT AGATGACACT CAGAAGGCTG 
     2701 CTGAGCTCAG TAAGGCAGCA CAGAGTGGGG AAGGGGACGA AGCCCTGGCG CCTACCCAGA 
     2761 CACTAGCAGA GAAGCCCACA GAGGGCCCTG AGGCCGGTGG GGCTGAGGAA GAGCCTCCTG 
     2821 GTGGAGAGAG AGTTGAGGAT AAACAGCCAG AGCAGCAGCC TGCAGTGTGT GAAGCTGAGG 
     2881 GACAGTTAAC CAGCACGTCA GAGACCACAC GGGCAACCCT GGAGCAACCG GAGACGGATG 
     2941 AAGTTGAGCA GGTCAGCGAG TCCAATAGCA TTGAGGAGCT AGAGAGACTT GTAGTTACTG 
     3001 GTGCTGAGGC ACGGGCTCTC GGGAGTGAAG GTGAGGCAGC TGCTACTGAG GTAGATTTGG 
     3061 AGAGAAAAGA AAACGCACAA AAAGTGCCCG TTAAAGCAGA GTCCCAAGCT CCTGCAGCAT 
     3121 CGCAGCCCAG CGAGCCTCAC CCTGTCTTAA TACCCAGTAT TAATATTAAT TCTGAAACCA 
     3181 CAGAAAATAA AGAAGAAATG GGTGCTTTGC CAAAACCTGA AACCATACTG CCACCAGAGC 
     3241 CTGAACATGA AAAGGGAAAT GACACCGACT CAGGGACTGG GTCCACTGTG GAGAATAGCA 
     3301 GTGGTGACCT GAACTTGTCC ATCTCTAGCT TCCTAAGCAA AGCTAAGGAC AGCGGCTCAG 
     3361 TGTCTCTGCA GGAGACAAGA AGACAGAAGA AAACATTGAA GAAAACACGC AAGTTTATTG 
     3421 TCGATGGTGT AGAAGTGAGT GTGACGACAT CGAAGATAGT TACAGACAGC GACTCCAAAA 
     3481 CGGAGGAACT GCGCTTTCTC AGGCGTCAGG AACTTCGGGA GCTGAGGCTT CTTCAGAAGG 
     3541 AGGAGCAGCG AGCCCAGCAG CAGCTCAATG GGAAACTGCA GCAGCAGCGG GAGCAGATCT 
     3601 TCAGGCGCTT TGAGCAGGAG ATGCTGAGTA AGAAGCGACA ATATGACCAA GAAATTGAGA 
     3661 ATTTAGAGAA GCAGCAGAAA CAGACAATTG AACGGCTAGA ACAAGAGCAC ACTAACCGCC 
     3721 TGAGAGACGA AGCCAAGCGC ATCAAAGGAG AGCAGGAGAA GGAGCTGTCC AAGTTCCAGA 
     3781 ATGTGCTGAA AAACCGCAAG AAGGAGGTTA TGAATGAAGT GGAGAAAGCC CCCAGAGAGC 
     3841 TGAGGAGGGA GCTCACGAAG CGCAGGAAAG AGGAGCTTGC GCAGAGCCAG CACGCTCAGG 
     3901 AACAAGAATT TGTTCAGAAG CAACAACAAG AGTTAGATGG TTCTCTGAAA AAGATTATCC 
     3961 AGCAGCAGAA GGCAGAGTTG GCCAATATTG AGAGAGAATG CCTGAATAAC AAGCAGCAGC 
     4021 TCATGAGAGC TCGAGAAGCC GCAATTTGGG AGCTTGAAGA GCGACATTTA CAAGAGAAGC 
     4081 ACCAGCTGCT TAAACAGCAG CTTAAAGATC AGTATTTCAT GCAGAGACAT CAGCTGCTAA 
     4141 AACGCCATGA GAAGGAAACA GAACAAATGC AGCGCTACAA TCAACGACTT ATTGAAGAAC 
     4201 TGAAAAACAG ACAGACTCAG GAACGAGCGA GACTGCCCAA GATTCAAAGA AGTGAAGCCA 
     4261 AGACACGAAT GGCCATGTTT AAAAAAAGTT TGAGGATCAA CTCAACAGCC ACACCAGACC 
     4321 AGGACCGTGA AAAAATTAAA CAGTTTGCTG CACAAGAAGA AAAGAGACAG AAAAATGAGA 
     4381 GAATGGCTCA GCATCAAAAA CATGAGAGCC AAATGCGGGA TCTTCAGTTG CAGTGTGAAG 
     4441 CCAATGTTCG GGAACTGCAC CAGCTGCAGA ATGAAAAATG CCACCTGTTG GTTGAACATG 
     4501 AGACTCAGAA GCTGAAGGAG TTGGATGAGG AGCACAGCCA AGAGCTGAAG GAGTGGAGAG 
     4561 AGAAGCTGAG ACCCAGGAAG AAGACACTGG AAGAAGAGTT TGCCAGGAAA CTGCAGGAAC 
     4621 AGGAAGTGTT CTTTAAAATG ACTGGGGAGT CCGAATGTCT TAATCCATCA GCACAGAGCC 
     4681 GGATCTCTAA ATTCTACCCT ATTCCCACCT TACATTCCAC TGGGTCAACG CGTACGCGGC 
     4741 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4801 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4861 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4921 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4981 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5041 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5101 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5161 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5221 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5281 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5341 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5401 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5461 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5521 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5581 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5641 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5821 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6241 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6301 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6361 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6421 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6481 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6541 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6601 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6661 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6721 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6781 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6841 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6901 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6961 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7021 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7081 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7141 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7201 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7261 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7321 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7381 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7441 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7501 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7561 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7621 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7681 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7741 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7801 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7981 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8041 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8101 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8221 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8341 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8461 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8521 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//