LOCUS       MR223804                8595 bp    DNA     circular  SYN 
DEFINITION  Kcnt1 (Myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 1
ACCESSION   NM_175462
VERSION     NM_175462.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4752..4843 
                     /label="Myc-DDK"  
     misc_feature    4873..5458 
                     /label="PolyA signal"  
     misc_feature    4930..4949 
                     /label="Restriction Enzyme"  
     misc_feature    5734..6406 
                     /label="ColE1"  
     misc_feature    6836..7630 
                     /label="Kan/Neo"  
     misc_feature    7665..7967 
                     /label="SV40 ori"  
     misc_feature    8053..8530 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4734..4740 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4734 
                     /label="Kcnt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCGGGCC AAGCTGCCGC GCTCGCCGTC CGAGGGCAAG GCGGGTCCGG 
     1081 GGGACACCCC AGCCGGCGCT GCAGCCCCTG AGGAGCCACA CGGGCTCAGC CCGCTACTGC 
     1141 CGGCCCGCGG CGGGGGCTCC GTGGGCAGCG ACGTGGGCCA GAGGCTTCAT GTGGAAGATT 
     1201 TCAGCCTGGA CTCTTCCCTT TCTCAGGTCC AGGTGGAGTT CTATGTCAAT GAGAACACCT 
     1261 TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG AGGATCCGGC 
     1321 TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT GTCCTGCTTG 
     1381 ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC ACTTTCAATG 
     1441 GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA ATGGCTCTGT 
     1501 GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC ATCATTTACC 
     1561 TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC TTGGAGATGA 
     1621 TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC CTGTTCATCC 
     1681 CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT AATGACTTCC 
     1741 ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC CTGTTCTGCA 
     1801 CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG CGAGCAGGTG 
     1861 GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA ACTGTGGGCT 
     1921 TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC CTGATCTGTG 
     1981 TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG ATGGAGCGTC 
     2041 AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC GTAGTCCTGT 
     2101 GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC TATGCCCATC 
     2161 CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC GTCCAGGTGC 
     2221 GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG GGCTCTGCCC 
     2281 TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC TTTATCCTCA 
     2341 GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT CGAGCCTGGG 
     2401 CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG CCCGAAAACA 
     2461 AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG TACGCCATGC 
     2521 TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG GTGCACACGT 
     2581 CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC GGGAGGTGCT 
     2641 CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG GAGTATGAGG 
     2701 GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG TGCCTCATCG 
     2761 GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG CACATCCTGG 
     2821 CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA GCTTTCATCT 
     2881 TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA TATGAAGGGC 
     2941 CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC ATGGACCTGC 
     3001 AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA AAGCTGACTC 
     3061 TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT CTGGAGTTGG 
     3121 CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG GATGAGGTGA 
     3181 CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT CCCCCCAACT 
     3241 CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA GCCCCCTTCT 
     3301 GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC AAGGCCTATG 
     3361 GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG CTCTACAACT 
     3421 TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT ATCGTGTTGC 
     3481 TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC CCCATGGTCT 
     3541 ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC ATCATCTATG 
     3601 CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG GACTACATGG 
     3661 CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC AGTCTCAGCA 
     3721 TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT GCCAAGGACA 
     3781 GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC TCCAACCTGG 
     3841 CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC ATGTTGGATA 
     3901 CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG CTGCTCTTGG 
     3961 GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC GAGGACGACC 
     4021 TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC GCCGAGATCC 
     4081 CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC GTCAGAGCCC 
     4141 AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC AAGGGACCCT 
     4201 GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG GGCAGTGCTG 
     4261 ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC AAGCTGAGTC 
     4321 GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC ACCCAGCAGC 
     4381 GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC AAGAACCGAA 
     4441 TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA ACAGCCAGTG 
     4501 ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT CATCACCAGA 
     4561 ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA CCCAACGACA 
     4621 TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC CAGAGTCGGA 
     4681 AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT GAGACCCAGC 
     4741 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4801 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4861 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4921 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4981 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5041 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5101 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5161 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5221 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5281 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5341 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5401 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5461 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5521 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5581 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5641 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5701 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5761 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5821 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5881 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5941 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6001 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6061 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6121 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6181 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6241 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6301 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6361 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6421 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6481 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6541 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6601 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6661 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6721 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6781 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6841 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6901 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6961 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7021 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7081 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7141 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7201 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7261 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7321 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7381 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7441 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7501 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7561 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7621 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7681 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7741 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7801 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8041 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8101 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8161 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8221 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8281 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8341 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8401 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8461 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8521 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8581 TTTAACGCGA ATTTT
//