LOCUS       MR223610                7554 bp    DNA     circular  SYN 
DEFINITION  Lpin1 (Myc-DDK-tagged) - Mouse lipin 1 (Lpin1), transcript variant 1
ACCESSION   NM_172950
VERSION     NM_172950.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3711..3802 
                     /label="Myc-DDK"  
     misc_feature    3832..4417 
                     /label="PolyA signal"  
     misc_feature    3889..3908 
                     /label="Restriction Enzyme"  
     misc_feature    4693..5365 
                     /label="ColE1"  
     misc_feature    5795..6589 
                     /label="Kan/Neo"  
     misc_feature    6624..6926 
                     /label="SV40 ori"  
     misc_feature    7012..7489 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3693..3699 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3693 
                     /label="Lpin1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTACGTG GGGCAGCTGG CCGGCCAGGT GTTTGTGACG GTGAAGGAGC 
     1081 TCTACAAGGG ACTGAACCCC GCCACGCTGT CGGGATGCAT CGACATCATT GTCATCCGGC 
     1141 AGCCCAATGG CAGTCTGCAG TGCTCCCCTT TCCACGTCCG CTTCGGCAAG ATGGGTGTCC 
     1201 TCCGCTCCCG AGAGAAAGTG GTGGACATAG AAATCAATGG GGAGTCCGTG GATTTGCACA 
     1261 TGAAGTTGGG AGACAACGGA GAAGCATTTT TTGTTCAAGA GACTGACAAC GATCAGGAAA 
     1321 TCATCCCCAT GTACCTGGCC ACGTCCCCCA TCCTGTCAGA AGGAGCTGCG AGAATGGAAA 
     1381 GCCAGCTGAA GAGGAACTCT GTAGACAGAA TCAGGTGCCT GGATCCCACT ACAGCTGCCC 
     1441 AGGGCCTGCC TCCCAGCGAC ACCCCATCCA CTGGTTCTCT GGGGAAGAAG AGAAGGAAAA 
     1501 GGAGGAGGAA GGCCCAGTTG GACAATCTCA AAAGAGATGA CAATGTCAAC ACATCTGAGG 
     1561 ATGAGGACAT GTTTCCCATA GAGATGAGCT CGGATGAGGA CACAGCACCG ATGGATGGAA 
     1621 GCAGAACTCT TCCTAATGAT GTACCACCAT TCCAAGATGA TATTCCTAAG GAAAACTTCC 
     1681 CCTCGATTTC AACGTACCCC CAGTCAGCAT CGTATCCCAG TTCGGACAGA GAATGGTCCC 
     1741 CCAGCCCCAG TCCTTCAGGC TCCCGGCCCT CAACACCAAA AAGTGACTCT GAGCTGGTCA 
     1801 GTAAGTCTGC AGACAGGTTG ACGCCAAAGA ATAACCTGGA AATGCTCTGG CTGTGGGGTG 
     1861 AATTGCCACA GGCTGCAAAG TCCTCTTCTC CACACAAGAT GAAAGAGTCC AGCCCCTTAG 
     1921 GGAGCCGGAA GACTCCTGAT AAAATGAATT TTCAGGCCAT TCACAGCGAG TCTTCAGATA 
     1981 CTTTTAGTGA CCAGTCGCCA ACAATGGCCC GGGGACTGCT CATCCACCAG AGTAAGGCCC 
     2041 AGACGGAAAT GCAGTTTGTG AACGAGGAGG ATCTCGAGTC CTTGGGGGCG GCAGCCCCAC 
     2101 CTTCACCCGT GGCCGAAGAG CTCAAGGCCC CATATCCCAA CACCGCACAG TCGTCGAGCA 
     2161 AGACAGATTC CCCTTCCAGG AAGAAAGATA AACGGAGCCG ACACCTTGGA GCTGATGGTG 
     2221 TTTATCTGGA CGACCTCACG GACATGGACC CTGAAGTGGC AGCCCTGTAT TTCCCCAAGA 
     2281 ATGGGGATCC TGGTGGGCTC CCCAAACAAG CCAGTGACAA CGGAGCCAGG TCAGCCAACC 
     2341 AGTCACCACA GTCTGTGGGA GGCTCGGGCA TCGACAGTGG TGTGGAGAGC ACCTCCGACA 
     2401 GCCTGAGGGA CCTGCCATCC ATCGCCATCT CCCTCTGCGG TGGCCTCAGT GACCACAGAG 
     2461 AGATCACCAA AGATGCATTT TTGGAACAAG CCGTGTCATA TCAGCAATTT GCCGACAACC 
     2521 CTGCTATCAT CGATGACCCC AACCTCGTGG TCAAGGTTGG CAATAAGTAT TACAACTGGA 
     2581 CAACAGCAGC TCCTCTACTT CTGGCGATGC AGGCTTTCCA GAAACCTTTG CCAAAGGCCA 
     2641 CTGTGGAATC CATCATGAGA GATAAGATGC CCAAAAAGGG AGGAAGATGG TGGTTTTCCT 
     2701 GGAGAGGAAG AAATGCCACA ATCAAAGAGG AAAGCAAGCC TGAACAGTGC CTGACTGGGA 
     2761 AAGGCCACAA TACCGGAGAG CAGCCTGCCC AGCTTGGCCT GGCCACCAGG ATAAAGCATG 
     2821 AGTCATCCTC CAGTGATGAA GAGCACGCAG CCGCCAAGCC ATCAGGTTCG AGCCACCTCT 
     2881 CTCTCTTGTC CAACGTCAGC TACAAAAAGA CCCTGCGGCT CACGTCGGAG CAGCTGAAAA 
     2941 GCTTGAAGTT GAAGAACGGC CCCAATGATG TGGTGTTCAG TGTCACTACC CAGTACCAGG 
     3001 GCACCTGTCG CTGCGAGGGC ACCATCTACC TGTGGAATTG GGACGACAAA GTCATCATCT 
     3061 CAGATATCGA TGGGACCATC ACAAGATCTG ATACTCTTGG TCACATTTTG CCCACGCTGG 
     3121 GAAAGGATTG GACTCACCAG GGCATTGCAA AGCTGTACCA CAAAGTAAGC CAGAATGGCT 
     3181 ACAAGTTTCT CTATTGTTCG GCACGTGCCA TTGGGATGGC GGACATGACG AGGGGCTACC 
     3241 TGCACTGGGT CAACGAGAGG GGCACGGTGC TTCCACAGGG CCCGCTTCTG CTCAGCCCGA 
     3301 GCAGCCTCTT CTCCGCCTTG CACAGAGAAG TGATTGAAAA GAAGCCAGAA AAGTTCAAAG 
     3361 TCCAGTGTTT GACAGACATC AAGAACCTGT TTTTCCCAAA CACAGAACCC TTCTATGCTG 
     3421 CTTTTGGGAA CCGGCCTGCT GATGTGTATT CCTACAAGCA AGTGGGAGTG TCCCTGAATA 
     3481 GGATCTTCAC TGTGAACCCC AAGGGTGAGC TGGTGCAGGA GCATGCCAAG ACCAACATCA 
     3541 GCTCGTACGT GCGGCTCTGC GAAGTGGTCG ATCACGTCTT CCCATTGCTA AAGAGAAGCC 
     3601 ATTCCTGTGA CTTCCCCTGT TCAGACACTT TCAGTAACTT CACCTTTTGG AGAGAGCCAC 
     3661 TGCCACCTTT TGAAAACCAG GACATGCATT CAGCCTCAGC TACGCGTACG CGGCCGCTCG 
     3721 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3781 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3841 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3901 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3961 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4021 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4081 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4141 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4201 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4261 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4321 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4381 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4441 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4501 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4561 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4621 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4681 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4741 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4801 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4861 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4921 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4981 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5041 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5101 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5161 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5221 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5281 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5341 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5401 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5461 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5521 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5581 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5641 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5701 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5761 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5821 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5881 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5941 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6001 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6061 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6121 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6181 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6241 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6301 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6361 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6421 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6481 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6541 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6601 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6661 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6721 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6781 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6841 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6901 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6961 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7021 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7081 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7141 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7201 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7261 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7321 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7381 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7441 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7501 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//