LOCUS       MR223518                8538 bp    DNA     circular  SYN 
DEFINITION  Nhsl2 (Myc-DDK-tagged) - Mouse NHS-like 2 (Nhsl2)
ACCESSION   NM_001163610
VERSION     NM_001163610.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="Nhsl2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTTCTAC CGGCGCACTG TGGTGCCCCA GCGTCTGTGC CCGCGCAACC 
     1081 CTCCGCAGCC GCTGGCTGAG CTCCGCGACG TGAGCCACCT GGCCGCGCTC AGTCTGCTCC 
     1141 GCCAGCTCGC AGACCTCTGC GGCCACTCGC TGGCTCTGCT GGAGGACCTC GAGGGGCACC 
     1201 TGCTGGCTCT GGGGCGCCGC ACCGACAGCC TGTACCGGCG CACCGTCCGG CTCCGCCGCC 
     1261 GCCTTCCCTG TCGCCTGCTT GGCCCGGAGG ATGATGAAGA ATTGCTAGCT GCAGCTAACT 
     1321 CGGGTCGGGA AAATGCGACA GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA 
     1381 ACGTGTTCCT CTCCTCGGGC AGGCCCCCGA GTGTAGAGGA ACTGCTTCGC GAGGCGCAGC 
     1441 TCAATCTCCA GAGCCTGTTG CAAGAAGAAT ATGAGGAACA GTATTCGGAG GCCAGACTTC 
     1501 TGGGGCAGAC CTTCCGCTCT TCTGATGGGG CCCCTGAGCC TACTCCCAGC CCAAGGCCCC 
     1561 AGTCTGCCAA GCGTCTGGAG TTTGTACTGA TGCCTGCAAA ACGGCAGCTG AGCGAGGATG 
     1621 AGACCACGAC CCAGGGTGTG AGGGCCCCTG AGGCCTGCCT GAGCCTGTCT ACTGCCAACA 
     1681 AGCAAAGTGC CTGGAATGAC CCCTTCCCTC TACCCATCCT AAAGGAGAGG CTCTGGCTTC 
     1741 AGCCCTGCTC CACACAGTCT GACCTTGTGC CAATCAACAT CTCTGGGCAG CAGTTTGATA 
     1801 GACATGCAAG TTTTCGACAC TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA 
     1861 CCCTGAGGCG GAGGCGGACC ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC 
     1921 ACAGCAGCAG CCCCACAGGC AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC 
     1981 ACTCAGCTCC ACAAGTTGTT CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA 
     2041 GTCTCACCTC ACCAGGACTG AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC 
     2101 GCAGTGCCTC AGACCATCCC GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT 
     2161 CTTGCAATGG ACCAACAGAG TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT 
     2221 ACATGACCCC AAGTGCCAGC AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT 
     2281 CCTCGGGCAA TTCTTGGGTC CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG 
     2341 CCACGCTCTT TGTCACCCGT GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG 
     2401 AGCACCCCAC TCTACGAAGA CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC 
     2461 GTGGTACCTC AAGGCTGGAA ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC 
     2521 TGTCTGTACC CACAGACTCG GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA 
     2581 GTGAGACCCG CATCCTGCCT TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA 
     2641 ACATTGCAGC CCTCAGCACT GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA 
     2701 TTTCACTCAA GAAGGCCAAA AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA 
     2761 AAGATGAGCC AGCCCTCCCA CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA 
     2821 GGAGCCTTTG CCTCTCCTTG GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG 
     2881 GTCACCCAGC TGTGCCAATG CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT 
     2941 ATCTTACTGA CTGGAAGTCT GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA 
     3001 CCGGCACCAC GGTCATTGAG TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC 
     3061 CTTCCACCTC CAGAGCGACA ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG 
     3121 TAGCCTCTCC TGGGAGGCCC ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT 
     3181 CAGAGACACC CACACCCACT GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA 
     3241 GTGCTAGTGT CAGAGTACGT CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT 
     3301 CACCAATGGA GAAAATCTGC AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA 
     3361 CCACCCTGGA TCTGTCGGGG ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC 
     3421 ATCACTCTGA TACCAATTTT GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA 
     3481 TCATGCCCAT GGTTACTCAG TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT 
     3541 CTGAGCCAGA AGACGACATT GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG 
     3601 TCTTCACCAT GCCAGAGAGA AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC 
     3661 GAAGGCCTCC AAGTTTGGTC CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT 
     3721 CTCCTACTAT GGCTATGGCA TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG 
     3781 ACAGCTACAC AGTGTTGCGG AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT 
     3841 CCTCCAGCCT TGTCCTCTCA CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC 
     3901 AGCAACCTCC CCAGGCCAGT GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA 
     3961 GAATCGGCCT CCAGAGCCAG GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG 
     4021 TACCAAAAAA GCCCAGTGTG CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG 
     4081 CCTGCATGGC AGAACCAAGG CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA 
     4141 AGTGTCCCTC CACTGGTGAT GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC 
     4201 ACACTGACAC AGAAAAAGAG GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG 
     4261 AGAAAAGTTT AATCAGTGAT AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT 
     4321 TTGTGGCTTC CCGCACAACT GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC 
     4381 TACTGGGCTG GAAAGAGACT GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC 
     4441 CAGTGAAGAA TACAGCTGAT TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA 
     4501 GCAGTGCCTG CCTAGATGCT GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG 
     4561 GAAGTAAGGC AACTCCCAGG ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC 
     4621 CTCTGGCTCG AAGGATTATT GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA 
     4681 GTACCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//