LOCUS       MR223400                8454 bp    DNA     circular  SYN 
DEFINITION  Prtg (Myc-DDK-tagged) - Mouse protogenin homolog (Gallus gallus) (Prtg)
ACCESSION   NM_175485
VERSION     NM_175485.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="Prtg"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCTCCC GTGCGCCCGG GGATGCTGCC CCTGCTGCTG TTGCTGCTAC 
     1081 TGCCGCCGCT CGGCTCGGTT CCAGGAGTGT GGAGCTTTAG CGAACTGTTT TTCATGAAAG 
     1141 AACCACAGGA TGCAACAGTC ACAAGGAAAG ACCCAGTCGT TTTAGATTGC CAGGCACACG 
     1201 GAGAAGGCCC CATTAAGGTC ACATGGTTGA AAAATGGAGC AAAATTGTCC GAAAATAAAC 
     1261 GGATCCAGGT TCTATCTAAC GGCTCTCTAT ACATCAGTGA GGTGGAAGGC AGGAGGGGAG 
     1321 AGCAATCGGA TGAAGGATTT TATCAGTGTT TGGCAGTGAA CAAATACGGG GCCATTCTTA 
     1381 GTCAGAAAGC TCATCTGACA TTGTCAACTA TCTCTGCGTT TGAAGTCCAT CCAGTGTCTA 
     1441 CTGAGGTCCA TGAAGGAGGA GTTGCTAGAT TTTCATGCAA GATTTCATCA ACGCCGCCTG 
     1501 CAGTCATAAC ATGGGAATTT AATAGAACAG CCCTGCCTAC AACCATGGAC AGGGTGACTG 
     1561 CCCTGCCATC AGGAGTGTTG CAGATCTACG ACGTTGGCCC GGAGGATGCT GGGAATTACC 
     1621 GCTGTGTTGC AGCTACTATA GCTCATAAGA GGAAAAGCAT GGAGGCTTCG CTAACCATAG 
     1681 TTGCAGCTAA TGAGACCAGA TCCTTCTACA TGCCAACCAT TATAGCCAGT CCACAGAATG 
     1741 TAACCGCATC TCTACATCAG ACAGTTGTGC TAGAGTGTAT GGCCACAGGA TATCCCAGAC 
     1801 CCATCATTTC CTGGAGCCGT CTTGATCACA AGTCCATTGA TGTCTTCAAC ACTCGGGTAC 
     1861 TTGGAAATGG CAACCTCATT ATATCAGATG TCAAGCTGCA GCATGCTGGA GTGTATGTTT 
     1921 GTCGGGCCAC CACCCCAGGC ACACGTAACT TCACAGTTGC TATGGCAACG TTAACTGTAT 
     1981 TAGCTCCTCC TTCATTTGTC GAATGGCCTG AAAGTTTAAC AAGGCCACGA GCTGGCACTG 
     2041 CACGATTTGT GTGCCAAGCA GAAGGAATTC CCTCACCCAA AATGTCCTGG TTGAAAAATG 
     2101 GGAGAAGGAT ACATTCAAAT GGCAGGATTA AAATGTACAA CAGCAAATTG GTCATTAATC 
     2161 AGATTATCCC GGAAGACGAT GCTATCTATC AGTGCATGGC TGAAAACAGC CAAGGATCTG 
     2221 TTCTGTCTCG AGCCAGACTG ACTGTGGTAA TGTCGGAAGA TAGACCCAGC GCTCCTTACA 
     2281 ACGTTCATGC TGAGACCATG TCGAGCTCTG CTATCCTTCT GGCTTGGGAG AGACCACTGT 
     2341 ACAATTCAGA CAAGGTCATC GCCTACTCTG TACACTACAT GAAGGCGGAA GGTTTAAATA 
     2401 ACGAGGAATA TCAGGTGGTC CTTGGAAACG ACACGACTCA TTACATCATT GATGACTTAG 
     2461 AACCCGACAG CAACTATACT TTCTACATTG TGGCGTACAT GCCGCTGGGG GCCAGCCAGA 
     2521 TGTCGGATCA TGTGACACAG AACACGCTAG AAGATGTGCC CCTAAGACCT CCCGAAATTA 
     2581 GCTTGACGAG TCGAAGTCCC ACCGACATTC TCGTCTCCTG GCTGCCAATC CCGGCCAAGT 
     2641 ACCGGCGGGG CCAAGTGGTG CTGTACCGCC TGTCCTTCCG CCTGAGCACT GAGAACGCCA 
     2701 TCCAAGTTGT AGAACTCCCG GGGACCGTGC ACGAGTACCT TCTGGAAGGC CTGAAGCCAG 
     2761 ACAGTGTCTA TCTGGTTCGG ATTACTGCAG CTACCCGTGT GGGGCTGGGA GAGTCGTCGG 
     2821 TGTGGACCTC ACACAGGACG CCCAAGGCTA CGAGCGTGAA AGCCCCGAAG TCTCCTGAAC 
     2881 TGCATTTGGA ACCACTGAAT TGTACTACCA TCTCTGTGAG GTGGCTGCAA GACACAGAGG 
     2941 ACCCGGCCGC TATTCGGGGC TACAAGCTGT TTTATAAAGA GGAAGGGCAG CAAGAACACG 
     3001 GACCTATCTT CCTAGACACC GGTGACCTGC TGTATACGCT CAGTGGCTTG GACCCCAGAA 
     3061 GAAAATACCA CGTGAGGCTC CTGGCTTACA ACAACATGGA AGAAGGCTAC CAGGCCGACC 
     3121 AGACTGTCAG CACTCCCGGA TGCGTGTCCG TCCGTGATCG CATGGTCCCT CCACCACCGC 
     3181 CACCCCACCA TCTCTACGCG AAGGCGAATA CCTCCTCTTC CATCTTCCTG CACTGGAGGA 
     3241 GGCCTGCGTT CACCACCGCC CAAGTCATTA ACTACACCAT CCGCTGTAAC CCTGTTGGCC 
     3301 TGCAGAATGC CTCTCTGGTT CTCTACCTGC AAACATCAGA AACCCACATG CTGGTCCAAG 
     3361 GACTAGAACC AAACACCAAG TATGAATTTG CTGTCCGACT GCATGTGGAT CAGCTGTCCA 
     3421 GTCCCTGGAG TCCTGTGGTC TACCATTCTA CATTGCCAGA AGCACCCACA GGCCCCCCGG 
     3481 TAGGGGTAAA GGTGACCCTG ATAGAAGACG ACACTGCCCT TGTTTCCTGG AAACCCCCAG 
     3541 ATGGCCCAGA GACAGTGGTG ACACGCTACA CCATCTTGTA TGCTTCCAGG AAGGCCTGGA 
     3601 TTGCGGGAGA GTGGCAGGTT CTTCACCGAG AAGGGGCAAT CACCATGGCT TTGCTGGAAA 
     3661 ACCTGGTGGC AGGGAACGTG TACATTGTCA AGATATCTGC GTCCAACGAG GTGGGAGAAG 
     3721 GGCCCTTTTC AAACTCCGTG GAGCTGGCCG TCCTCCCCAA GGATGCTTCA GAGTCGAACC 
     3781 AGAGGCCCAA GCGTTTGGAT TCTTCCAATG CCAAAGTTTA TTCCGGCTAT TACCATCTGG 
     3841 ACCAAAAGTC AATGACGGGC ATCGCAGTAG GCGTTGGCAT AGCCTTGACC TGTATCCTCA 
     3901 TCTGTGTTCT CATCTTGATC TACCGAAGCA AAGCCAGGAA GTCATCCGCC TCTAAGACAG 
     3961 CGCAGAGTGG AACCCAGCCG TTATCCCAAG CCAGTGCCTC TGTAGCAGCA GGGAGTGACA 
     4021 TGGGGAAGAA CCTGGAGAGA GCTACAGAGA CTGCAGAGTC CTTGGTACCC ATGATGCCCA 
     4081 GCAGCTTCAT TGACGCGAAG GGAGGGACTG ACCTGATCAT CAATAGCTAC GGTCCTATAA 
     4141 TTAAAAACAA CACTAAGAAA AAGTGGCTTT TTTTCCAAGA CACTAAGAAA ATCAAAGTTG 
     4201 AACAGACTCA AAGAAGATTT ACTCAGGCCG TCTGCTTTTA CCAGCCGGGC ACCACCGTGC 
     4261 TGATCAGCGA TGAAGATTCC CCCGGCTCCC CAGGCCAGAC CGCCAGCTTC CCAAGACCTT 
     4321 TTGGGGCTAC TGCCCTTGAC ACAGAGCATT CAGCGAACAG TGAAGGCAGC CATGAGACAG 
     4381 GGGATTCCGG AAGGTTTTCC CATGAGTCCA ATGATGAGAT ACACCTGTCC TCCGTCATCA 
     4441 GCAGCACGCC CCCCACCTCG AATCCCCTCG CTGGTGGCGA TTCTGATGGG GATGCTGCAC 
     4501 CGAAGAAGCA CGGAGACCCT GCGCAGCCCT TGCCAGCTGA GCAGACCTCA GCTCCGCAGA 
     4561 CATCTGCGGG GCTGCGATAT GCTGCCGAGG GTTTTCCGGT CACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//