LOCUS       MR223373                7881 bp    DNA     circular  SYN 
DEFINITION  Flt3 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3)
ACCESSION   NM_010229
VERSION     NM_010229.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4038..4129 
                     /label="Myc-DDK"  
     misc_feature    4159..4744 
                     /label="PolyA signal"  
     misc_feature    4216..4235 
                     /label="Restriction Enzyme"  
     misc_feature    5020..5692 
                     /label="ColE1"  
     misc_feature    6122..6916 
                     /label="Kan/Neo"  
     misc_feature    6951..7253 
                     /label="SV40 ori"  
     misc_feature    7339..7816 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4020..4026 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4020 
                     /label="Flt3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCGTTG GCGCAGCGCA GCGACCGGCG GCTGCTGCTG CTTGTTGTTT 
     1081 TGTCAGTAAT GATTCTTGAG ACCGTTACAA ACCAAGACCT GCCTGTGATC AAGTGTGTTT 
     1141 TAATCAGTCA TGAGAACAAT GGCTCATCAG CGGGAAAGCC ATCATCGTAC CGAATGGTGC 
     1201 GAGGATCCCC AGAAGACCTC CAGTGTGCCC CGAGGCGCCA GAGTGAAGGG ACGGTATATG 
     1261 AAGCGGCCAC CGTGGAGGTG GCCGAGTCTG GGTCCATCAC CCTGCAAGTG CAGCTCGCCA 
     1321 CCCCAGGGGA CCTTTCCTGC CTCTGGGTCT TTAAGCACAG CTCCCTGGGC TGCCAGCCGC 
     1381 ACTTTGATTT ACAAAACAGA GGAATCGTTT CCATGGCCAT CTTGAACGTG ACAGAGACCC 
     1441 AGGCAGGAGA ATACCTACTC CATATTCAGA GCGAAGCCGC CAACTACACA GTACTGTTCA 
     1501 CAGTGAATGT AAGAGATACA CAGCTGTACG TGCTAAGAAG ACCTTACTTT AGGAAGATGG 
     1561 AAAACCAGGA CGCACTGCTC TGCATCTCCG AGGGTGTTCC AGAGCCCACT GTGGAGTGGG 
     1621 TGCTCTGCAG CTCCCACAGG GAAAGCTGTA AAGAAGAAGG CCCTGCTGTT GTCAGAAAGG 
     1681 AGGAAAAGGT ACTTCATGAG TTGTTCGGAA CAGACATCAG ATGCTGTGCT AGAAATGCAC 
     1741 TGGGCCGCGA ATGCACCAAG CTGTTCACCA TAGATCTAAA CCAGGCTCCT CAGAGCACAC 
     1801 TGCCCCAGTT ATTCCTGAAA GTGGGGGAAC CCTTGTGGAT CAGGTGTAAG GCCATCCATG 
     1861 TGAACCATGG ATTCGGGCTC ACCTGGGAGC TGGAAGACAA AGCCCTGGAG GAGGGCAGCT 
     1921 ACTTTGAGAT GAGTACCTAC TCCACAAACA GGACCATGAT TCGGATTCTC TTGGCCTTTG 
     1981 TGTCTTCCGT GGGAAGGAAC GACACCGGAT ATTACACCTG CTCTTCCTCA AAGCACCCCA 
     2041 GCCAGTCAGC GTTGGTGACC ATCCTAGAAA AAGGGTTTAT AAACGCTACC AGCTCGCAAG 
     2101 AAGAGTATGA AATTGACCCG TACGAAAAGT TCTGCTTCTC AGTCAGGTTT AAAGCGTACC 
     2161 CACGAATCCG ATGCACGTGG ATCTTCTCTC AAGCCTCATT TCCTTGTGAA CAGAGAGGCC 
     2221 TGGAGGATGG GTACAGCATA TCTAAATTTT GCGATCATAA GAACAAGCCA GGAGAGTACA 
     2281 TATTCTATGC AGAAAATGAT GACGCCCAGT TCACCAAAAT GTTCACGCTG AATATAAGAA 
     2341 AGAAACCTCA AGTGCTAGCA AATGCCTCAG CCAGCCAGGC GTCCTGTTCC TCTGATGGCT 
     2401 ACCCGCTACC CTCTTGGACC TGGAAGAAGT GTTCGGACAA ATCTCCCAAT TGCACGGAGG 
     2461 AAATCCCAGA AGGAGTTTGG AATAAAAAGG CTAACAGAAA AGTGTTTGGC CAGTGGGTGT 
     2521 CGAGCAGTAC TCTAAATATG AGTGAGGCCG GGAAAGGGCT TCTGGTCAAA TGCTGTGCGT 
     2581 ACAATTCTAT GGGCACGTCT TGCGAAACCA TCTTTTTAAA CTCACCAGGC CCCTTCCCTT 
     2641 TCATCCAAGA CAACATCTCC TTCTATGCGA CCATTGGGCT CTGTCTCCCC TTCATTGTTG 
     2701 TTCTCATTGT GTTGATCTGC CACAAATACA AAAAGCAATT TAGGTACGAG AGTCAGCTGC 
     2761 AGATGATCCA GGTGACTGGC CCCCTGGATA ACGAGTACTT CTACGTTGAC TTCAGGGACT 
     2821 ATGAATATGA CCTTAAGTGG GAGTTCCCGA GAGAGAACTT AGAGTTTGGG AAGGTCCTGG 
     2881 GGTCTGGCGC TTTCGGGAGG GTGATGAACG CCACGGCCTA TGGCATTAGT AAAACGGGAG 
     2941 TCTCAATTCA GGTGGCGGTG AAGATGCTAA AAGAGAAAGC TGACAGCTGT GAAAAAGAAG 
     3001 CTCTCATGTC GGAGCTCAAA ATGATGACCC ACCTGGGACA CCATGACAAC ATCGTGAATC 
     3061 TGCTGGGGGC ATGCACACTG TCAGGGCCAG TGTACTTGAT TTTTGAATAT TGTTGCTATG 
     3121 GTGACCTCCT CAACTACCTA AGAAGTAAAA GAGAGAAGTT TCACAGGACA TGGACAGAGA 
     3181 TTTTTAAGGA ACATAATTTC AGTTTTTACC CTACTTTCCA GGCACATTCA AATTCCAGCA 
     3241 TGCCTGGTTC ACGAGAAGTT CAGTTACACC CGCCCTTGGA TCAGCTCTCA GGGTTCAATG 
     3301 GGAATTTAAT TCATTCTGAA GATGAGATTG AATATGAAAA CCAGAAGAGG CTGGCAGAAG 
     3361 AAGAGGAGGA AGATTTGAAC GTGCTGACGT TTGAAGACCT CCTTTGCTTT GCGTACCAAG 
     3421 TGGCCAAAGG CATGGAATTC CTGGAGTTCA AGTCGTGTGT CCACAGAGAC CTGGCAGCCA 
     3481 GGAATGTGTT GGTCACCCAC GGGAAGGTGG TGAAGATCTG TGACTTTGGA CTGGCCCGAG 
     3541 ACATCCTGAG CGACTCCAGC TACGTCGTCA GGGGCAACGC ACGGCTGCCG GTGAAGTGGA 
     3601 TGGCACCTGA GAGCTTATTT GAAGGGATCT ACACAATCAA GAGTGACGTC TGGTCCTACG 
     3661 GCATCCTTCT CTGGGAGATA TTTTCACTGG GTGTGAACCC TTACCCTGGC ATTCCTGTCG 
     3721 ACGCTAACTT CTATAAACTG ATTCAGAGTG GATTTAAAAT GGAGCAGCCA TTCTATGCCA 
     3781 CAGAAGGGAT ATACTTTGTA ATGCAATCCT GCTGGGCTTT TGACTCAAGG AAGCGGCCAT 
     3841 CCTTCCCCAA CCTGACTTCA TTTTTAGGAT GTCAGCTGGC AGAGGCAGAA GAAGCGATGT 
     3901 ATCAGAACAT GGGTGGCAAC GTCCCAGAAC ATCCATCCAT CTACCAAAAC AGGCGGCCCC 
     3961 TCAGCAGAGA GGCAGGCTCA GAGCCGCCAT CGCCACAGGC CCAGGTGAAG ATTCACGGAG 
     4021 AAAGAAGTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4081 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4141 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4201 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4261 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4321 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4381 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4441 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4501 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4561 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4621 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4681 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4741 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4801 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4861 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4921 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4981 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5041 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5101 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5161 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5221 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5281 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5341 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5401 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5461 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5521 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5581 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5641 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5701 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5761 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5821 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5881 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5941 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6001 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6061 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6121 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6181 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6241 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6301 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6361 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6421 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6481 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6541 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6601 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6661 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6721 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6781 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6841 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6901 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6961 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7021 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7081 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7141 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7201 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7261 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7321 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7381 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7441 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7501 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7561 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7621 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7681 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7741 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7801 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7861 CAAAAATTTA ACGCGAATTT T
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