LOCUS       MR223263                7896 bp    DNA     circular  SYN 
DEFINITION  Espnl (Myc-DDK-tagged) - Mouse espin-like (Espnl)
ACCESSION   NM_001033292
VERSION     NM_001033292.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4053..4144 
                     /label="Myc-DDK"  
     misc_feature    4174..4759 
                     /label="PolyA signal"  
     misc_feature    4231..4250 
                     /label="Restriction Enzyme"  
     misc_feature    5035..5707 
                     /label="ColE1"  
     misc_feature    6137..6931 
                     /label="Kan/Neo"  
     misc_feature    6966..7268 
                     /label="SV40 ori"  
     misc_feature    7354..7831 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4035..4041 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4035 
                     /label="Espnl"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGCAG CAGGCACTGG TGGCCTCCAA GGATGGGGAT ATGGCCACCT 
     1081 TGGAACGGCT ATTTGAGGCT GGTGCCCTAA GACCAGACAT CACTGATGAT CTGGGGGCTG 
     1141 GCCTGGTGCA TCATGCCACC CGGGCTGGTC ACCTGGACTG TGTCAAGTTC CTGGTACAGA 
     1201 GAGCCAAGCT GCCAGGCAAC CAGCAGGCAC ACAACGGGGC CACACCAGTG CACGATGCAG 
     1261 CAGCCACGGG CAACCTGGCA GAGCTGTGCT GGCTGGTCCG TGATGCGGGC TGCGGGCTGC 
     1321 AGGACCAAGA TGCCTCAGGT GTCTCTCCGC TGCACCTGGC TGCCCGGTTT GGACACCCAG 
     1381 CCCTGGTGGA GTGGTTACTC CGTGAGGGCC ATGCAGCCAC ACTGGAGACG CTGGAAGGGG 
     1441 CCTTACCACT GCACCACGCT GCTGTAAGTG GTGATCTGAC CTGTCTGAAA CTCCTGACAG 
     1501 CGGCCCACAG CAGTGGTGTG AACCAGCGGA CATGCAGTGG TGCCTCTCCA CTCTACCTGG 
     1561 CCTGCCAAGA GGGCCACCTG CATCTGGCAC AGTTCCTGGT GAAAGACTGC GGGGCCGACG 
     1621 TGCGCCTGAG AGCCCTGGAT GGCATGAGCT CATTGCACGC TGCGGCTGCC CACGGCCACT 
     1681 ACTCTCTAGT TGTCTGGCTG GTGACATTCA CAGACATCGG CCTGACAGCA CGGGATAATG 
     1741 AAGGAGCCAC TGCCCTGCAC TTTGCAGCTC GGGGGGGCCA CACACCTATT CTCGACCGGC 
     1801 TCCTGCTCAT GGGTGCCCCC ATCATGAGAG ACTCTTGGGG AGGGACTCCC CTGCATGATG 
     1861 CAGCCGAGAA TGGACACATG GAGTGCTGCC AGACTCTGCT CTCCCACCAC GTAGACCCCT 
     1921 TCCTGCGGGA TGAAGATGGG TATACAGCGA TAGACCTGGC AGAGTACCAT GGACACCAGG 
     1981 ATTGTGCCCA GTTCCTGCGG GAAATGTCTC GGCCGGTGCG GGTCCTGATG ACACCCCCAC 
     2041 CCCCACCATT TCCACCTCCT CCACTGCTGG CTGCAAAACT CTCCCTGGAA GAGGAAAGAA 
     2101 GAGGGGACTC GGGACTCAAA AGCCCCTCAT CTGCAACTCT CAGCCCAGTC TGGCCTGCTC 
     2161 AGCCTGTCCC AAGGGAACCG ATGGCCTGCA CGGCCCCTCT GAGGGTCACC ACCCCAGATG 
     2221 CTCTTCAGGG GCCTGAGGTG GAAGCCCGGG ACTCCCGTGC TGGCCTAGCC ACTCTGCAGC 
     2281 TGGATGGCTT GCCTGCGGGA GATCTTGACA TGCTGGTGCC CACACAGGAC GAGCGTGGCC 
     2341 GGCCCATCCC TGAGTGGAAG CGGCAGGTGA TGGTGCGGAA GCTACAGGCT CGCCTGGGTG 
     2401 CAGACCATCC TCCAGAAGAC CAGGACCAGA GTCAGAGGCA GGACTCAGGG CCCACCGCTG 
     2461 CAGAACAGGC AACTTGGAGA TATTCACAGA CGCACCAGGC CATCCTGGGC CCCTTTGGAG 
     2521 AGCTGCTGAC GGAGGATGAT CTTGTATACC TGGAGAAGCA GATCAACGAC CTGCAGCTCC 
     2581 GGCGACGCTG CCAGGAGTAT GAGAGTGAGT TGGGCCGGCT GGCAGCCCAG CTGCAGGCCC 
     2641 TCCTGCCCGA GCCCCTGGTC AGCATCACCG TCAACAGCCA CTTTCTGCCC CGGGCTCCCG 
     2701 GCCTGGAAGA CGAGGAAGCT CCAGTCCTGG CGACTGAACT CGAAGCCTCC GAGGAGCCGG 
     2761 GGAAGGCCGA GCCCAGAGGG CAGCCGCTGC CCTTCTGGTG CAGCCACATC GGTCGCCTGG 
     2821 TGCGCAGCAT GTCCCTGCTG CTGAAGGGTA TGAACGGGCT GGCACAGGGT GAGGAGAAAC 
     2881 CACCCTCACC GGTCCCGCAG GACCTAGGCA AGGAGACCAT TGCCGGCCCC TCCCGGAGCG 
     2941 AGGCCCAGCG CGAGATCCAA GAGTGCGGAG TGTCAGTTCG GACATTGCGG GGGAACTTCG 
     3001 AGTTTGCCCC TGATCTGCCC TGCGCCTTGA ACTCGGGTCC CTGTGAGCTG GGGGTGCGGC 
     3061 CTGGCCAGTG TCTGAGAGGT TGCTGGTCGG CGCCGCCACA GCCCCGAGGC GACGCGATGG 
     3121 GTGGGGAGCC CGGGCCAGGC GACACAGAGG AGGCCAGCGA CTCGGGCATC AGCTGCGAAG 
     3181 AGGCCCCGTC AGAGGCCGGC GCGGGTCCAG GGCTGGACCT GGCCAGCTTG CGCAAGGAGC 
     3241 GCATCGTCAT GCTCTTCCTC GGCCATTGGA AGAAGTCAGC CTACACGCCC GCGCTCCGGA 
     3301 CGGCAGCCTG CAGGACCCTG GAGGCTCAGC GTGCACGATC CAGGGGCCCA GAGGCCGCGG 
     3361 GTAGCCCCCG GCCGTCCTCC CCGCAGCCCA GCGATGGCCC TCGGCTTGGC CACCTGTGGC 
     3421 AGCAGCGTGG CATCATCACC CACCTGCTGG GCACCTGGAA AGCCATCATG GCGCACGTGC 
     3481 CCGCCCGGCA GCTGCGGAGG CTGAGCCGCA GGGAGCGTGG GCCCCTGTCT CCGGAGCAGT 
     3541 TCTTGCCGCA CGTGGACGGG GCCCCCGTGC CCTACAACAG CCTCTCGCTG GACCTCTTCA 
     3601 TGCTTGGCTA CTTCCAGCTC CTGGAGTGCG ACCTGCCAGC CGAGGAGCGC AAGATGCGCC 
     3661 ACCTGCTGTG CTTCGAGGTT TTCGAGCATC TGGGCGCACA CGGCTGGGAG GCTGTGCGAG 
     3721 CCTTCCATAA GGCAGTGACC GACGAGGTGG CCGCGGGTCG CCGCGCCTGG ACCGACGGCT 
     3781 TTGAGGACAT CAAAGCCCGC TTCTTCGGCT CCAGCCAGGG TCCCCCCTGG GACGTGGAGC 
     3841 CAGGTCGCAA GCTGGGCCTG ACACCACTCG GGTCCTTGCC CCATGCTTCC CTTCCTGGCA 
     3901 GCGGCCCTGA ACCTGCGGTG CCGCCGAGGC TGGGGTCTGA TTCCCAGGGG AGCAGCTTCA 
     3961 ACAGCGGAGA CATCTGTGGC TACATCGACC GCAGCTTCGC ATTCTGGAAG GAGAAAGAGG 
     4021 CTGAGATGTT TAACTTTGGA GAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4081 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4141 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4201 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4261 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4321 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4381 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4441 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4501 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4561 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4621 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4681 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4741 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4801 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4861 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4921 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4981 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5041 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5101 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5161 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5221 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5281 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5341 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5401 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5461 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5521 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5581 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5641 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5701 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5761 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5821 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5881 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5941 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6001 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6061 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6121 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6181 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6241 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6301 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6361 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6421 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6481 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6541 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6601 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6661 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6721 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6781 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6841 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6901 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6961 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7021 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7081 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7141 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7261 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7321 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7381 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7441 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7501 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7561 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7621 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7681 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7741 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7801 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7861 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//