LOCUS       MR223095                8232 bp    DNA     circular  SYN 
DEFINITION  Zeb1 (Myc-DDK-tagged) - Mouse zinc finger E-box binding homeobox 1 (Zeb1)
ACCESSION   NM_011546
VERSION     NM_011546.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4389..4480 
                     /label="Myc-DDK"  
     misc_feature    4510..5095 
                     /label="PolyA signal"  
     misc_feature    4567..4586 
                     /label="Restriction Enzyme"  
     misc_feature    5371..6043 
                     /label="ColE1"  
     misc_feature    6473..7267 
                     /label="Kan/Neo"  
     misc_feature    7302..7604 
                     /label="SV40 ori"  
     misc_feature    7690..8167 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4371..4377 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4371 
                     /label="Zeb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATGGC CCCAGGTGTA AGCGCAGAAA GCAGGCGAAC CCGCGGCGCA 
     1081 ATAACGTTAC AAATTATAAT ACTGTGGTAG AAGCAAATTC AGATTCCGAT GATGAAGACA 
     1141 AACTCCATAT TGTGGAAGAA GAAAGTATTA CAGATGCAGC TGACTGTGAA GGTGGCATGC 
     1201 CAGATGATGA ACTGCCAGCA GACCAGACAG TATTACCAGG AGGCAGTGAC AGGGGGGGCG 
     1261 GTGCCAAGAA CTGCTGGCAA GACAACGTGA AAGACAACGA GTGTGACTCA GATGCAGAAA 
     1321 ATGAGCAAAA CCATGATCCG AATGTGGAAG AATTTCTGCA GCAACAAGAC ACCGCCGTCA 
     1381 TTTATCCTGA GGCGCCCGAG GAAGACCAGC GGCAGGGCAC ACCAGAAGCC AGCAGTCATG 
     1441 ATGAAAACGG AACACCAGAT GCATTTTCCC AGTTGCTCAC CTGCCCGTAT TGTGATAGAG 
     1501 GCTACAAGCG CTTTACCTCT TTGAAAGAAC ACATTAAGTA CCGCCATGAG AAGAACGAGG 
     1561 ACAACTTCAG CTGCTCCCTG TGCAGTTACA CCTTTGCATA CAGAACCCAG CTTGAACGTC 
     1621 ATATGACATC ACATAAGTCA GGAAGAGAGC AAAGACATGT GACACAGTCT GGGGGAAACC 
     1681 GCAAGTTCAA GTGCACTGAA TGCGGGAAGG CCTTCAAGTA CAAACACCAC CTGAAAGAGC 
     1741 ACTTACGGAT TCACAGTGGA GAGAAGCCAT ACGAATGCCC GAACTGCAAG AAACGGTTTT 
     1801 CCCATTCTGG CTCCTATAGC TCACATATAA GCAGTAAGAA GTGTATTAGC TTGATGCCTG 
     1861 TGAATGGCAG GCCTAGATCA GGACTCAAGA CATCTCAGTG TTCCTCGCCA TCTCTTTCGA 
     1921 CATCACCAGG CAGTCCCACA CGCCCACAGA TACGACAGAA GATAGAGAAT AAACCCCTTC 
     1981 AAGAACCGCT TTCTGTAAAC CAAATCAAAA CTGAACCTGT GGATTATGAG TTCAAACCCA 
     2041 TAGTGGTTGC TTCAGGAATC AACTGTTCAA CCCCTTTACA AAATGGGGTT TTTAGCAGTG 
     2101 GTGGCCAATT GCAGGCAACC AGTTCTCCTC AGGGTGTGGT GCAAGCCGTT GTTCTGCCAA 
     2161 CAGTTGGTTT GGTATCTCCC ATAAGTATCA ACTTAAGTGA CATTCAGAAT GTACTTAAAG 
     2221 TGGCTGTAGA TGGTAACGTA ATACGACAAG TCTTGGAGAC TAATCAAGCC AGTCTTGCAT 
     2281 CCAAAGAGCA AGAAGCAGTG AGTGCTTCGC CCATCCAGCA GGGTGGCCAT TCTGTCATTT 
     2341 CTGCCATCAG TCTTCCTTTA GTTGATCAGG ATGGAACAAC CAAAATCATC ATCAACTACA 
     2401 GTCTTGAGCA GCCCAGTCAA CTTCAGGTTG TTCCCCAGAA TTTAAAGAAA GAAATCCCAG 
     2461 CCCCTACAAA CAGCTGCAAA AGTGAGAAGT TACCAGAAGA CCTTACTGTC AAATCAGAAA 
     2521 CGGACAAAAG CTTTGAGGGG GCCAGGGATG ATAGCACTTG CCTTCTGTGT GAGGACTGCC 
     2581 CAGGGGACCT CAATGCACTT CCAGAACTAA AGCACTATGA CCCAGAGTGC CCTGCTCAGC 
     2641 CTCCACCCCC TGCCCCAGCC ACCGAGAAGC CAGAGTCCTC TGCTTCATCA GCTGGAAACG 
     2701 GAGATTTGTC TCCCAGTCAG CCACCTTTAA AGAACCTTCT GTCACTCTTG AAAGCCTACT 
     2761 ATGCTCTGAA CGCGCAGCCA AGCACAGAAG AGCTCTCAAA GATCGCCGAT TCTGTGAACC 
     2821 TACCGCTGGA TGGAGTTAAA AAGTGGTTTG AAAAGATGCA AGCTGGACAG ATTCCAGGAC 
     2881 AGTCTCCTGA CCCCCCTTCT CCTGGAACCG GGTCAGTAAA CATACCTACA AAAACCGATG 
     2941 AGCAGCCTCA ACCTGCGGAT GGAAATGAGC CCCAGGAAGA CAGCACACGC GGACAGAGTC 
     3001 CTGTCAAGAT AAGGAGCTCT CCGGTTTTAC CTGTAGGATC AGCCATGAAC GGTTCCAGAA 
     3061 GCTGCACATC ATCCCCATCC CCTCTAAACC TTTGCTCAGC CAGGAACCCG CAGGGTTACT 
     3121 CTTGTGTGGC AGAGGGTGCC CAGGAGGAGC CCCAAGTAGA ACCTCTTGAT CTCTCACTAC 
     3181 CAAAGCAACA GGGAGAGTTA CTGGAAAGGT CGACAGTCAG TAGCGTTTAC CAGAACAGTG 
     3241 TTTATTCTGT CCAGGAAGAA CCCTTGAACT TGTCTTGTGC AAAAAAGGAA CCACAAAAGG 
     3301 ACAGCTGTGT TACAGACTCA GAACCAGTTG TAAATGTAGT CCCACCAAGT GCCAACCCCA 
     3361 TAAACATTGC TATTCCTACA GTCACTGCCC AGTTACCCAC AATCGTGGCC ATTGCTGACC 
     3421 AGAACAGTGT TCCATGTTTA AGAGCACTGG CCGCCAACAA GCAGACTATT CTGATTCCCC 
     3481 AAGTGGCATA TACATATTCA GCTACTGTGA GCCCTGCCGT GCAGGAGCCG CCAGTGAAGG 
     3541 TGATCCAGCC AAACGGAAAC CAGGATGAAA GACAAGACAC TAGCTCAGAA GGAGTCTCCA 
     3601 CTGTGGAGGA CCAGAATGAC TCTGACTCCA CGCCACCCAA AAAGAAAACT CGGAAGACAG 
     3661 AGAATGGAAT GTATGCATGT GACCTGTGTG ACAAGATATT TCAGAAGAGC AGCTCACTGT 
     3721 TGAGACACAA ATATGAGCAC ACAGGTAAGA GGCCTCACGA GTGTGGAATC TGTAGAAAGG 
     3781 CATTTAAACA CAAGCATCAT TTGATTGAGC ACATGCGGCT GCACTCTGGG GAAAAGCCCT 
     3841 ATCAATGTGA CAAGTGTGGC AAGCGCTTCT CACACTCCGG CTCCTACTCT CAACACATGA 
     3901 ATCACCGCTA CTCCTACTGC AAGAGAGGAG CTGAAGACAG AGATGCTATG GAGCAGGAAG 
     3961 ACGCTGGGCC CGAAGTCCTG CCGGAAGTCC TGGCGACTGA GCATGTTGGT GCCCGGGCGT 
     4021 CTCCCTCACA GGCTGACTCG GACGAGAGAG AAAGTCTGAC AAGGGAAGAA GATGAAGACA 
     4081 GTGAAAAGGA GGAGGAGGAG GAAGATAAAG AGATGGAAGA ATTACAGGAA GGAAAGGAAT 
     4141 GTGAGAACCC ACAGGGGGAG GAGGAGGAGG AGGAGGAGGA GGAAGAGGAG GAAGAAGAGG 
     4201 AGGAAGAGGA GGAAGTGGAA GCGGATGAAG CCGAGCATGA GGCAGCAGCC AAGACTGATG 
     4261 GTACAGTGGA GGTTGGAGCT GCACAGCAGG CAGGCAGCTT AGAGCAGAAG GCCAGCGAGA 
     4321 GCGAGATGGA GAGCGAAAGC GAGAGTGAGC AGCTGTCTGA AGAGAAGACA AATGAAGCTA 
     4381 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4441 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4501 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4561 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4621 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4681 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4741 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4801 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4861 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4921 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4981 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5041 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5101 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5161 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5221 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5281 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5341 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5401 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5461 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5521 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5581 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5641 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5701 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5761 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5821 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5881 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5941 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6001 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6061 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6121 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6181 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6241 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6301 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6361 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6421 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6481 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6541 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6601 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6661 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6721 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6781 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6841 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6901 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6961 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7021 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7081 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7141 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7201 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7261 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7321 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7381 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7441 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7681 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7741 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7801 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7861 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7921 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7981 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8041 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8101 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8161 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8221 AACGCGAATT TT
//