LOCUS       MR222229                8916 bp    DNA     circular  SYN 
DEFINITION  Disp2 (Myc-DDK-tagged) - Mouse dispatched homolog 2 (Drosophila) (Disp2)
ACCESSION   NM_170593
VERSION     NM_170593.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5073..5164 
                     /label="Myc-DDK"  
     misc_feature    5194..5779 
                     /label="PolyA signal"  
     misc_feature    5251..5270 
                     /label="Restriction Enzyme"  
     misc_feature    6055..6727 
                     /label="ColE1"  
     misc_feature    7157..7951 
                     /label="Kan/Neo"  
     misc_feature    7986..8288 
                     /label="SV40 ori"  
     misc_feature    8374..8851 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5055..5061 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5055 
                     /label="Disp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCTGAG GCAAGCCCAG AGAGAAGCTG CTCCCTCCAC ACCTGTCCCC 
     1081 TTGAAGACCC AACCGGTGCT CCGGTACCAC CACCAACCGT GTCCACGCTC CAGGCTATAG 
     1141 ACCCGACGAG TCCGTTAACG GCTGGTCATT TTGCCTTTCC TCGAGCGCCT CAAGACTATC 
     1201 AAGAAGGAAG TTCATTATTA GGATTAGGAG ACCAAGCATC TCTATGTGCC CATGTCTCCA 
     1261 ACCTCAGCAC CTCCATAGAC ACCTCTCAGC ATGATGGGGT CTGGAAGCAA CCTTCTGTGC 
     1321 AGCGCCATGT GGTCAGTGTC AGGCAGGAAC GCACTTTCCG GATGCCGAAG AGCTATTCTC 
     1381 ATATGATTGC TGATTGGCCA GTGGCTGTGA TCGTGGGGTG TCTGGCCTTC ATCTTCCTCT 
     1441 GTACCCTAGC TGGGCTGTTG GGAAGCCCAC CACTTGACTT TTCTGAGCCT TTACTGGGTT 
     1501 TTGAGCCTCG AGACACAGAG ATAGGCCGAA GGTTAGAAGT TTGGAAAGCC ATGCAGGCCC 
     1561 TCACTGGCCC CAAGAATCTG CTTTCTCTTT CCCCAGACCC TGAGATGAAC AGCTCAAGCC 
     1621 TCCTCAGCAC CCTGAGCCCT GCAGCCTGGG GCAGGGCCGA GGAAAGTGTG GTCCGGACCA 
     1681 AGAGGATGGT GGGGCCTGTG GAAGTCAAAG AAGAAGAGAA CTTCTTTTGT GGCCGCCCTG 
     1741 AGAAGAGCCA TGCAAAGCTG GTGTTTGTGT CCACCTCTGG GGGAAGCCTG TGGAACCTGC 
     1801 AAGCCATCCA TTCCATGTGT CGCATAGAAC AGGAGCAGAT CCGCTCTCAC ATCAGTTTTG 
     1861 GGGCTCTGTG CCAGCGATCA GCAGCCAATG AGTGCTGCCC CAGCTGGTCC TTGGGCAACT 
     1921 ATCTGGCCGT GCTGTCTAAC CGCTCCTCCT GCCAAGACAC TACCCAAGCC GATACAGACC 
     1981 GCACATTGGC CCTGCTACGG TTCTGTGCCA CCTTCTACCA TCGTGGTGTC CTGGTGCCTG 
     2041 CTTGTGTGGG GTCTAGCCAG GACAAACCCC CGTTTTGTGC CCAAGTTCCT GCCAAGTGTA 
     2101 CCGGAAGCAA CGTGGTCTAT GAATTCCTGC ACTACCTGCT AGACAGAGAC TTTCTGAGTC 
     2161 CCCAGACTGC GGATTACCAG GTGCCCTCCC TCAAGTTTGC CCTGCTCTTC CTGCCCATTA 
     2221 TAAAGACCTC CTCTCTTCTA GACATCTACC TGGACGGCCT AGGTGACCCA ATTAAAGTCT 
     2281 CTGACAACTA CACATCTATC AGTGGCATGG ACTTGGGCCT CAAGCCCAGA CTGCTGAAGT 
     2341 ATTACCTAGC CGAAGACACC ATGTATCCCT TGATAGCCCT GGTTGTCATC TTCTTTGGCA 
     2401 TGTCCCTCTA CCTGCGCTCA CTCTTCATCA CGTTCATGTC ACTTCTAGGG GTGCTGGGCT 
     2461 CCCTAATGGT GGCCTACTTT CTTTACCATG TGGCATTCCG CATGGCCTAC TTCCCCTTTG 
     2521 TCAATCTAGC GGCTCTCCTT CTGCTTAGTG GTGTCTGTGT CAATTACACG CTCATCTTCT 
     2581 TGGATATGTG GCGCCTCAGC AGGGGTCAGG TGCCTTCCGG GGGCATGCCA CACCGTGTAG 
     2641 GCCGTACCAT GCACCACTTT GGCTACCTGC TCCTGGTCTC AGGCCTCACC ACCAGCGCAG 
     2701 CCTTCTACGG CAGCTACCTG AGCCGCCTGC CCGCAGTGCG TTGCTTTGCT CTTTTCATGG 
     2761 GCACTGCTGT ACTAGTGCAC ATGGGGCTCA CACTGCTCTG GCTTCCCGCC ACCGTGGTGC 
     2821 TCCATGAGCG CTACTTGGCT CACGGCTGTG TGGCCCAAGC GCACGGCCAG AGGGGTGGCA 
     2881 GCGACCCCCT GAGGCTGTTG CTGGCGTTGC ACAGACGGAT CCGTATTTTT CGCAAGATTA 
     2941 TTTCCATCCT CTCGCGCCTG CTTTTCCAGC GCCTGCTGCC CTGTGGTGTC ATTAAGTTTC 
     3001 GGTACATCTG GATCTGCTGG TTCGCGGCGT TGGCGGCAGG GGGCGCCTAC ATCGGCGGCG 
     3061 TCAGCCCTCG CCTGCAACTG CCCATTCTAT TGCCACTTGG AGGCCAGTTC TTCCGTTCTA 
     3121 GCCATCCCTT TGAACGCTTT GACGCAGAGT ATCGCCAGCA GTTCCTGTTC GAGGATCTGC 
     3181 CTCCAAACGA GGGCGGCAAC TTGCCAGTGG TTCTGGTGTG GGGAATCCTG CCAGTGGACA 
     3241 CTAGTGATCC CCTGGACCCT CGCACCAACA GCTCAGTGGT AAGTGATCCT GACTTCTCGC 
     3301 CTAGCAGCCC GGAGGCCCAG GAGTGGCTCC TGGCTCTCTG CCATGGAGCC CAGAATCAGA 
     3361 GCTTCTTTGG AGATCAGCCA GAGGGCTGGC CTACGCTGTG TTTAGTGGAA GCCTTACAGC 
     3421 AGTGGATGGA GAGCCCTAGC TGTGGCCGCC TGGGTCCTGA TCTATGCTGC GGACAATCAG 
     3481 AATTCCCCTG GGCCCCCCAG CTTTACCTGC ACTGTCTCAA GATGATGGCT CTGGAGCAAA 
     3541 GTCCTGATGG CACACGTGAC CTGGGACTCC GCTTCGATAC TCATGGCAAC CTGGCAGCTC 
     3601 TAGTTCTAAA GTTCCAGACC AACTTACCCT ACAGTACAGA GTACGGCCCG GTCCACCACT 
     3661 TCTACACTGA AATCAGCCGC TGGTTGTCAA CCGAGATGAG CAAGGCACCT CCTGGCCTCA 
     3721 ACCAGGGTTG GTTCACCAGC AACTTGGAGC TGTACAGCTT GCAGCATAGT CTAAGCACAG 
     3781 AGCCAGCTGT CGTGCTTGGT CTGGCCCTGG CACTAGCCTT TGCCACACTG CTGCTGAGCA 
     3841 CCTGGAATGT CCCCCTCAGT CTGTTCTCTG TGGCAGCTGT GGCTGGCACC GTACTGCTCA 
     3901 CGGTGGGCTT GCTGGTTCTT CTTGAGTGGC AACTCAACAC TGCCGAAGCT CTCTTTCTCT 
     3961 CTGCCTCTGT GGGCCTCTCT GTAGACTTAA CCATTAACTA CTGCATCTCC TATCACTTGT 
     4021 GCCCACACCC GGACCGCCTG AGCCGTGTGG CCTTCTCTTT ACGTCAGATC AGCCGGGCCA 
     4081 CGGCGATGAC GACTGGAGTG TTGTTTGCCT CTGGTGTGAT CATGCTGCCT TCCACTATAC 
     4141 TGCTCTATCG GAAGCTGGGC ATCATCGTCA TGATGGTCAA GTTCCTTGGC TGTGGCTTTG 
     4201 CCAGCTTCTT CTTTCAGTCC CTGTGCTGTT TCTTCGGGCC AGAAAAGAAC TGTGGCCAGA 
     4261 TCCTCTGGCC CTGCGCCCAT CTGCCGTGGG ATGCCGGGAC TGAGGATCCT GACGAGAAGG 
     4321 GGCGAGCGGG GCCACCAGGA TTCTCCGAAC ACTATGAGTT GCAGCCCCTG GCACGGCGCC 
     4381 GGAGTCCCAG CTTCGACACC AGCACAGCCA CCAGCAAGCT TTCCCATCGG CCTTCCATAC 
     4441 TCTCTGAAGA CCTGCAGATA CATGATGGCA GCTGCTGCCT CCAGCATGCC CAAGCCCCTG 
     4501 TCTCCCCAAG GGATCTGCTC CTAGACCACC AGACAGTCTT CAGCCAGTGT CCAGCCCTGC 
     4561 AGACCTCCTC TCCATATAAG CAGGCTGGCC CCACCCCCCA AACCTGGATC AGGCAAGATT 
     4621 CCCAGGGACA GAAAACTGAG CCCCTGCAGG CACTGCCAGA AGGCCCTGCC CACTGCCCTA 
     4681 AGTCCAAAGT GGAAGAGCTC CCCGATGGCC TGTGCTCCTC AGCCAGCACC CTGGAGGGAC 
     4741 TCAGCGTCTC AGATGACACC TGTGCCTCTG AGCATAGTGT CCGTGTGCCA GATTCTGTGG 
     4801 GTACCTCCCC AGAAGTCATG AATGGCACTG GACACCCCAT ACTTGAGCGG GGCCAGCTGA 
     4861 ATGGGAAGCG CGACACCCTC TGGCTAGCAC TGAAGGAGAC CATCTATGAC CCAAACATGC 
     4921 CCAATTCTCA CCACAGCAGC TTATCCTGGA AGGGCCGTGG AGGGCCAGGC GATATCAGCC 
     4981 CTGTGATGCT TCCCAACAGT CAGCCAGATC TCCCAGATGT TTGGCTCCGT AGGCCCAGCA 
     5041 CCTACACCTC TGGCTACAGC AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5101 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5161 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5221 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5281 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5341 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5401 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5461 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5521 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5581 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5641 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5701 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5761 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5821 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5881 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5941 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6001 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6061 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6121 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6181 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6241 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6301 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6361 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6421 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6481 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6541 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6601 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6661 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6721 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6781 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6841 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6901 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6961 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7021 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7081 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7141 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7201 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7261 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7321 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7381 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7441 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7501 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7561 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7621 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7681 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7741 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7801 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7861 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7921 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7981 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8041 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8101 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8161 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8281 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8341 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8401 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8461 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8521 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8581 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8641 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8701 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8761 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8821 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8881 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//