LOCUS       MR221940                7059 bp    DNA     circular  SYN 
DEFINITION  Pnn (Myc-DDK-tagged) - Mouse pinin (Pnn)
ACCESSION   NM_008891
VERSION     NM_008891.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3216..3307 
                     /label="Myc-DDK"  
     misc_feature    3337..3922 
                     /label="PolyA signal"  
     misc_feature    3394..3413 
                     /label="Restriction Enzyme"  
     misc_feature    4198..4870 
                     /label="ColE1"  
     misc_feature    5300..6094 
                     /label="Kan/Neo"  
     misc_feature    6129..6431 
                     /label="SV40 ori"  
     misc_feature    6517..6994 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3198..3204 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3198 
                     /label="Pnn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTCGCC GTGAGAGCTT TGCAGGAGCA GCTAGAGAAG GCCAAAGAGA 
     1081 GCCTCAAGAA TGTGGACGAG AATATCCGCA AGCTCACCGG GCGGGACCCG AATGATGTGA 
     1141 GGCCCATCCA AGCCAGATTG CTGGCCCTTT CTGGTCCTGG TGGAGGTAGA GGACGAGGTA 
     1201 GCTTATTGCT AAGGCGTGGA TTCTCAGATA GTGGAGGAGG ACCCCCAGCC AAACAAAGAG 
     1261 ACCTGGAAGG GGCAGTCAGT AGGCTGGGTG GGGAGCGTCG GACAAGAAGA GAATCACGCC 
     1321 AGGAGAGCGA CCCAGAAGAC GATGATGTTA AAAAGCCAGC ACTGCAATCT TCCGTTGTAG 
     1381 CTACCTCCAA AGAGCGCACG CGCAGAGACC TTATCCAGGA TCAAAACATG GATGAGAAGG 
     1441 GGAAGCAAAG GAACCGACGA ATATTTGGCT TGTTGATGGG CACTCTTCAG AAATTTAAAC 
     1501 AAGAATCTAC TGTTGCTACT GAACGGCAAA AAAGACGCCA AGAAATTGAA CAAAAACTTG 
     1561 AAGTTCAGGC TGAAGAAGAG AGAAAGCAGG TTGAAAACGA GAGGCGAGAA CTGTTTGAGG 
     1621 AGAGACGTGC TAAGCAGACG GAGCTGCGGC TTCTGGAGCA GAAGGTTGAG CTTGCGCAGC 
     1681 TGCAAGAAGA ATGGAATGAA CATAATGCCA AGATAATTAA GTATATAAGG ACCAAGACAA 
     1741 AGCCCCATTT GTTCTATATT CCTGGAAGAA TGTGTCCAGC TACCCAAAAG CTAATAGAAG 
     1801 AGTCACAGAG AAAGATGAAT GCTTTATTTG AAGGTAGACG CATCGAATTT GCAGAACAAA 
     1861 TAAATAAAAT GGAGGCTAGG CCTAGAAGAC AATCAATGAA GGAAAAAGAG CATCAGGTGG 
     1921 TGCGTAATGA AGAACAGAAG GCGGAACAAG AAGAGGGTAA GGTGGCTCAG CGAGAGGAAG 
     1981 AGTTGGAGGA GACAGGTAAT CAGCACAATG ATGTAGAAGT AGAGGAGGCA GGAGAGGAAG 
     2041 AGGAGAAGGA AGCAGGTATA GTTCATAGTG ATGCAGAGAA AGAACAGGAG GAGGAGGAAC 
     2101 AAAAGCAGGA AATGGAGGTT AAGACTGAGG AGGAGGCTGA GGTAAGAGAA GGTGAGAAGC 
     2161 AGCAGGATAG TCAGCCAGAA GAAGTGATGG ATGTGCTAGA GATGGTGGAA AGTGTCAAAC 
     2221 ACGTAATTGC TGAGCAGGAA GTAATGGAAA CTAACCAAGT TGAAAGTATA GAGCCTTCAG 
     2281 AAAATGAAAC TAGCAAAGAA TTGGAGCCAG AGATGGAATT TGATGTTGAG CCAGATAAAG 
     2341 AATGTAAATC TCTTTCTCCT GGAAAAGAGA ATATTAATTC TCAGGAAGTG GAAAAGGAGT 
     2401 CTGAGGAGAA AGAGGAAAAA GAAGAGAAAG AACCTGAGCC CCAACCTGAG CCTGTGGCTC 
     2461 AGCCTCAGCC CCCACCTCAG CCCCTGCCCC AGAGCCAGCC CCATTCTCAG CCCCACTCCC 
     2521 AGCCCCAGCC TGTACTCCAG TCCCAGCCTC TCTGTCAGCC TGAGACTTTG CCATTAGCTG 
     2581 TTTTGCAGCC TCCACCTCAG GTTATTCAGG AGCAAGGGAA TCTACTACCT GAGCGGAAGG 
     2641 ATTTTCCTCT AGAGTCTATA AAACTCCCTG AGGTGTCTGT AGAGCCAGTC TTGACAGTAC 
     2701 ATTCAGAAAA CAAAAGCAAG AATAAGACTA GGAGCAGGAG CAGGGGGCGA GCAAGGAATA 
     2761 AAACAAGCAA GAGTAGAAGT CGGAGCAGCA GCAGCAGTAG TTCCAGTAGC AGCTCTACTA 
     2821 GTAGCAGCAG CGGGAGCAGC TCCAGCAGTG GCAGCAGCAG TAGTCGAAGC AGCTCCAGCA 
     2881 GCAGCTCCAG CACGAGTGGG AGCAGCAGCA GAGACAGCAG CAGCAGCACT AGCAGTAGCA 
     2941 GTGAGAGTAG GAGTCGCAGT AGGGGCCGAG GACATAACCG AGATAGGAAG CACAGAAGGA 
     3001 GCGTGGACCG GAAGCGAAGG GATACTTCAG GACTAGAAAG AAGTCACAAG TCTTCAAAAG 
     3061 GTGGTAGTAG TAGAGATACA AAAGGATCAA AGGATAAGAG TTCCCGCCCT GACAGAAAAA 
     3121 GGTCTATATC AGAGAGTAGT CGATCAGGCA AAAGATCTTC AAGAAGTGAA AGAGACCGAA 
     3181 AATCAGACAG GAAAGACAAA AGGCGTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3241 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3301 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3361 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3421 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3481 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3541 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3601 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3661 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3721 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3781 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3841 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3901 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3961 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4021 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4081 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4141 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4201 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4261 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4321 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4381 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4441 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4501 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4561 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4621 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4681 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4741 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4801 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4861 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4921 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4981 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5041 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5101 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5161 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5221 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5281 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5341 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5401 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5461 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5521 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5581 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5641 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5701 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5761 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5821 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5881 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5941 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6001 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6061 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6121 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6181 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6241 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6301 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6361 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6421 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6481 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6541 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6601 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6661 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6721 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6781 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6841 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6901 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6961 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7021 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//