LOCUS       MR221909                8850 bp    DNA     circular  SYN 
DEFINITION  Tnik (Myc-DDK-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 3
ACCESSION   NM_001163008
VERSION     NM_001163008.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5007..5098 
                     /label="Myc-DDK"  
     misc_feature    5128..5713 
                     /label="PolyA signal"  
     misc_feature    5185..5204 
                     /label="Restriction Enzyme"  
     misc_feature    5989..6661 
                     /label="ColE1"  
     misc_feature    7091..7885 
                     /label="Kan/Neo"  
     misc_feature    7920..8222 
                     /label="SV40 ori"  
     misc_feature    8308..8785 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4995 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="Tnik"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCAGCTC GCAGCCTGGA TGAAATCGAT CTCTCCGCCC 
     1081 TGAGGGACCC TGCAGGGATC TTTGAGTTGG TGGAACTTGT CGGAAATGGC ACGTATGGTC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC TGCCATTAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACAGGAA CATTGCTACA TACTACGGTG CTTTTATCAA AAAGAACCCT CCTGGCATGG 
     1321 ATGACCAACT CTGGTTGGTT ATGGAGTTCT GTGGTGCTGG CTCTGTCACT GACCTGATCA 
     1381 AGAACACGAA AGGCAACACA TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAGATCT 
     1441 TACGGGGCCT GAGTCACCTG CACCAGCACA AAGTGATTCA TCGAGATATC AAAGGGCAGA 
     1501 ACGTCTTGTT GACTGAAAAT GCAGAGGTTA AGCTAGTGGA TTTTGGAGTG AGTGCCCAGC 
     1561 TTGACCGAAC TGTGGGCAGG AGGAACACGT TCATCGGGAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTCATTGC CTGTGATGAG AACCCGGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGAAT CACCGCCATT GAGATGGCAG AAGGTGCCCC CCCCCTCTGT GACATGCATC 
     1741 CCATGAGAGC CCTCTTCCTC ATCCCACGGA ACCCTGCACC TCGGCTCAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATCGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGGC 
     1861 CAGCCACGGA GCAGTTGATG AAGCACCCAT TCATACGAGA CCAACCTAAT GAGAGGCAGG 
     1921 TCCGCATCCA GCTGAAGGAC CACATTGATC GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACTGAGTA TGAATACAGC GGAAGTGAGG AAGAAGAGGA AGAGAATGAC TCTGGGGAAC 
     2041 CCAGCTCCAT TCTGAACCTA CCAGGGGAGT CAACACTGCG AAGGGACTTC CTGAGACTGC 
     2101 AGCTGGCCAA CAAGGAGCGC TCAGAGGCCC TGCGGCGCCA ACAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA AGAACACAAG CGGCAGCTAC TGGCTGAGCG CCAGAAGCGC ATCGAAGAGC 
     2221 AGAAGGAGCA AAGGCGGAGG CTGGAGGAGC AACAAAGGCG AGAAAAAGAG CTTCGGAAAC 
     2281 AGCAGGAGCG GGAACAGCGC CGGCACTACG AAGAACAGAT GCGTCGGGAG GAGGAGAGGA 
     2341 GGCGTGCCGA ACATGAGCAG GAATATAAGC GCAAACAATT GGAAGAACAG AGACAAGCAG 
     2401 AAAGACTGCA GAGGCAGCTA AAGCAAGAGC GGGACTATCT GGTTTCCCTC CAGCATCAGC 
     2461 GGCAGGAGCA GAGGCCCCTG GAGAAGAAGC CACTGTACCA TTACAAGGAG GGCATGAGTC 
     2521 CTAGTGAGAA GCCGGCCTGG GCCAAGGAGG TAGAAGAACG CTCAAGACTC AACCGACAGA 
     2581 GTTCACCTGC CATGCCTCAC AAGGTTGCCA ACAGGATCTC GGACCCCAAC CTGCCCCCAA 
     2641 GATCAGAGTC CTTCAGCATT AGTGGGGTTC AGCCTGCAAG GACACCCCCA ATGCTCAGAC 
     2701 CTGTTGACCC CCAGATCCCG CAGCTGGTAG CTGTCAAATC CCAGGGACCT GCCTTGACGG 
     2761 CCTCCCAGTC AGTACATGAG CAACCCACAA AGGGCCTGTC TGGGTTCCAG GAGGCTCTGA 
     2821 ATGTGACCTC TCACCGGGTC GAGATGCCAC GCCAGAACTC GGATCCCACC TCAGAAAACC 
     2881 CTCCTCTCCC CACGAGAATT GAGAAGTTTG ACAGAAGCTC TTGGTTACGA CAGGAAGAAG 
     2941 ACATTCCACC AAAGGTGCCT CAAAGAACAA CTTCTATATC CCCAGCACTA GCCAGAAAGA 
     3001 ATTCCCCTGG CAATGGCAGT GCTCTGGGCC CCAGACTTGG ATCTCAGCCC ATCAGAGCAA 
     3061 GCAACCCTGA TCTGCGCAGG ACAGAGCCAG TCCTGGAGAG TTCCCTGCAG CGGACAAGCA 
     3121 GTGGCAGTTC CTCCAGCTCC AGCACTCCCA GCTCCCAGCC CAGCTCCCAA GGAGGCTCTC 
     3181 AACCTGGCTC CCAAGCAGGA TCTAGTGAGC GGTCCAGAGT GCGGGCCAAC AGTAAGTCCG 
     3241 AAGGATCACC CGTGCTCCCC CATGAGCCTT CCAAGGTGAA ACCAGAAGAA TCCAGAGACA 
     3301 TCACACGGCC CAGTCGGCCA GCTGATCTGA CGGCATTAGC CAAAGAATTA CGAGAACTCC 
     3361 GCATTGAAGA AACAAACCGC CCCCTGAAGA AAGTGACTGA TTACTCTTTC TCCAGCGAGG 
     3421 AGTCGGAGAG CAGTGAGGAA GAAGAGGAAG ATGGAGAGAG TGAGACACAT GACGGGACGG 
     3481 TGGCTGTCAG TGACATACCC AGACTAATAC CCACCGGAGC TCCAGGGAAC AATGAGCAGT 
     3541 ACAACATGGG GATGGTCGGG ACACATGGGC TGGAAACTTC GCATGCGGAC ACCTTTGGCG 
     3601 GCAGCATTTC AAGAGAAGGA ACCTTGATGA TCAGAGAGAC GGCTGAAGAG AAGAAGCGAT 
     3661 CTGGCCACAG TGACAGTAAT GGATTCGCCG GTCACATCAA TCTCCCAGAC CTTGTACAGC 
     3721 AGAGCCATTC GCCAGCTGGA ACTCCCACTG AGGGGCTGGG CCGCGTCTCC ACTCATTCCC 
     3781 AGGAGATGGA CTCTGGGGCT GAATATGGTA TAGGGAGCAG CACCAAAGCC TCTTTCACCC 
     3841 CCTTCGTGGA CCCTCGAGTG TACCAGACAT CGCCCACTGA TGAAGATGAA GAGGATGATG 
     3901 AGTCTTCAGC TGCTGCCCTG TTTACTAGCG AACTTCTTAG GCAAGAACAG GCCAAACTCA 
     3961 ATGAAGCGAG GAAGATTTCA GTGGTAAATG TGAACCCAAC AAACATTCGC CCTCATAGTG 
     4021 ACACACCGGA AATCAGAAAA TACAAGAAAC GCTTCAATTC AGAAATACTT TGTGCAGCTC 
     4081 TATGGGGTGT GAACCTTCTG GTGGGGACTG AAAATGGCCT GATGCTTTTG GACAGAAGTG 
     4141 GCCAAGGCAA AGTCTACAAC CTAATCAACC GGAGGCGGTT TCAGCAGATG GATGTGCTAG 
     4201 AAGGACTAAA TGTTCTTGTC ACGATATCAG GAAAGAAGAA CAAGCTCCGT GTGTACTATC 
     4261 TCTCATGGTT AAGAAACAGA ATCCTGCACA ATGACCCAGA AGTGGAAAAG AAGCAGGGCT 
     4321 GGATCACTGT CGGTGACTTG GAAGGCTGCA TCCATTACAA AGTCGTTAAA TATGAAAGAA 
     4381 TCAAGTTCCT GGTGATTGCC TTAAAGAATG CAGTAGAGAT ATATGCGTGG GCCCCTAAAC 
     4441 CTTACCATAA GTTCATGGCA TTTAAGTCTT TTGCAGATCT TCAGCATAAG CCTCTGCTTG 
     4501 TTGACCTCAC AGTAGAAGAA GGTCAAAGGT TAAAGGTCAT ATTTGGCTCA CACACTGGTT 
     4561 TCCATGTAAT TGATGTTGAT TCTGGAAACT CCTACGATAT CTATATACCA TCCCATATTC 
     4621 AGGGCAATAT CACTCCTCAT GCTATCGTCA TCTTGCCTAA AACAGACGGA ATGGAGATGC 
     4681 TTGTCTGCTA TGAGGATGAA GGGGTGTACG TGAACACCTA CGGCCGGATC ACTAAGGATG 
     4741 TGGTGCTCCA ATGGGGAGAA ATGCCCACAT CTGTGGCCTA CATTCATTCC AATCAGATAA 
     4801 TGGGCTGGGG CGAGAAAGCT ATTGAGATCC GGTCAGTGGA AACAGGACAT TTGGATGGAG 
     4861 TGTTTATGCA TAAACGAGCT CAAAGGTTAA AGTTTCTATG TGAAAGAAAT GATAAGGTAT 
     4921 TTTTTGCATC CGTGCGATCT GGAGGAAGTA GCCAAGTGTT TTTCATGACC CTCAACAGAA 
     4981 ATTCCATGAT GAACTGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5041 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5101 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5161 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5221 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5281 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5341 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5401 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5461 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5521 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5581 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5641 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5701 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5761 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5821 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5881 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5941 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6001 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6061 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6121 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6181 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6241 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6301 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6361 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6421 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6481 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6541 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6601 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6661 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6721 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6781 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6841 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6901 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6961 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7021 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7081 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7141 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7201 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7261 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7321 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7381 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7441 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7501 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7561 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7621 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7681 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7741 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7801 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7861 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7921 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7981 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8041 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8101 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8281 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8341 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8401 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8461 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8521 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8581 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8641 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8701 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8761 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8821 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//