LOCUS       MR221659L1                9105 bp    DNA     circular  SYN 
DEFINITION  Hcn1 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 1 (Hcn1)
ACCESSION   NM_010408
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7396..7576 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8304..8963 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7735..8303 
                     /label="ColE1"  
     misc_feature    6271..6291 
                     /label="LR50 primer"  
     misc_feature    6068..6139 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6280..6871 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6050..6056 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6050 
                     /label="Hcn1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAGGCGGC GGCAAACCCA ACTCCGCGTC 
     3361 CAACAGCCGC GACGATGGCA ACAGCGTCTT CCCCTCCAAG GCGCCCGCGA CGGGGCCGGT 
     3421 GGCGGCCGAC AAGCGCCTGG GGACCCCGCC GGGGGGCGGC GCGGCCGGGA AGGAACATGG 
     3481 CAACTCCGTG TGCTTCAAGG TGGACGGCGG CGGAGGAGAG GAGCCGGCGG GCAGCTTCGA 
     3541 GGATGCCGAG GGGCCCCGGC GGCAGTATGG TTTCATGCAG AGGCAGTTCA CCTCCATGCT 
     3601 GCAGCCTGGG GTCAACAAAT TCTCCCTCCG CATGTTTGGG AGCCAGAAGG CGGTGGAGAA 
     3661 GGAGCAGGAA AGGGTTAAAA CTGCAGGCTT CTGGATTATC CATCCGTACA GTGACTTCAG 
     3721 GTTTTATTGG GATTTAATCA TGCTTATAAT GATGGTTGGA AATTTGGTCA TCATACCAGT 
     3781 TGGAATCACG TTCTTCACAG AGCAGACGAC AACACCGTGG ATTATTTTCA ACGTGGCATC 
     3841 CGATACTGTT TTCCTGTTGG ACTTAATCAT GAATTTTAGG ACTGGGACTG TCAATGAAGA 
     3901 CAGCTCGGAA ATCATCCTGG ACCCTAAAGT GATCAAGATG AATTATTTAA AAAGCTGGTT 
     3961 TGTGGTGGAC TTCATCTCAT CGATCCCGGT GGATTATATC TTTCTCATTG TAGAGAAAGG 
     4021 GATGGACTCA GAAGTTTACA AGACAGCCAG AGCACTTCGT ATCGTGAGGT TTACAAAAAT 
     4081 TCTCAGTCTC TTGCGGTTAT TACGCCTTTC AAGGTTAATC AGATACATAC ACCAGTGGGA 
     4141 AGAGATATTC CACATGACCT ATGACCTCGC CAGTGCTGTG GTGAGGATCT TCAACCTCAT 
     4201 TGGCATGATG CTGCTTCTGT GCCACTGGGA TGGCTGTCTT CAGTTCCTGG TTCCCCTGCT 
     4261 GCAGGACTTC CCACCAGATT GCTGGGTTTC TCTGAATGAA ATGGTTAATG ATTCCTGGGG 
     4321 AAAACAATAT TCCTACGCAC TCTTCAAAGC TATGAGTCAC ATGCTGTGCA TTGGTTATGG 
     4381 CGCCCAAGCC CCTGTCAGCA TGTCTGACCT CTGGATTACC ATGCTGAGCA TGATTGTGGG 
     4441 CGCCACCTGC TACGCAATGT TTGTTGGCCA TGCCACAGCT TTGATCCAGT CTTTGGACTC 
     4501 TTCAAGGAGG CAGTATCAAG AGAAGTATAA GCAAGTAGAG CAATACATGT CATTCCACAA 
     4561 GTTACCAGCT GACATGCGCC AGAAGATACA TGATTACTAT GAGCACCGAT ACCAAGGCAA 
     4621 GATCTTCGAT GAAGAAAATA TTCTCAGTGA GCTTAATGAT CCTCTGAGAG AGGAAATAGT 
     4681 CAACTTCAAC TGCCGGAAAC TGGTGGCAAC TATGCCTCTT TTTGCTAACG CCGATCCCAA 
     4741 TTTCGTGACG GCCATGCTGA GCAAGCTGAG ATTTGAGGTG TTCCAGCCCG GAGACTATAT 
     4801 CATTCGAGAA GGAGCTGTGG GGAAGAAAAT GTATTTCATC CAGCACGGTG TTGCTGGCGT 
     4861 TATCACCAAG TCCAGTAAAG AAATGAAGCT GACAGATGGC TCTTACTTCG GAGAGATATG 
     4921 CCTGCTGACC AAGGGCCGGC GCACTGCCAG TGTCCGAGCT GATACCTACT GTCGTCTTTA 
     4981 CTCCCTTTCG GTGGACAATT TCAATGAGGT CTTGGAGGAA TATCCAATGA TGAGAAGAGC 
     5041 CTTTGAGACA GTTGCTATTG ACCGACTCGA TCGGATAGGC AAGAAAAACT CTATTCTCCT 
     5101 GCAGAAGTTC CAGAAGGATC TAAACACTGG TGTTTTCAAC AACCAGGAGA ACGAGATCCT 
     5161 GAAGCAGATC GTGAAGCATG ACCGAGAGAT GGTACAAGCT ATCCCTCCAA TCAACTATCC 
     5221 TCAAATGACA GCCCTCAACT GCACATCTTC AACCACCACC CCAACCTCCC GCATGAGGAC 
     5281 CCAATCTCCG CCAGTCTACA CCGCAACCAG CCTGTCTCAC AGCAATCTGC ACTCACCCAG 
     5341 TCCCAGCACA CAGACGCCCC AACCCTCAGC CATCCTTTCA CCCTGCTCCT ATACCACAGC 
     5401 AGTCTGCAGT CCTCCTATAC AGAGCCCCCT GGCCACACGA ACTTTCCATT ATGCCTCTCC 
     5461 CACTGCGTCC CAGCTGTCAC TCATGCAGCA GCCTCAGCAG CAACTACCGC AGTCCCAGGT 
     5521 ACAGCAGACT CAGACTCAGA CTCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA 
     5581 GCAACAGCAA CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA 
     5641 GCAGCAGCAG CAGCCACAGA CACCTGGTAG CTCCACACCG AAAAATGAAG TGCACAAGAG 
     5701 CACACAAGCC CTTCATAACA CCAACCTGAC CAAAGAAGTC AGGCCCCTTT CCGCCTCGCA 
     5761 GCCTTCTCTG CCCCATGAGG TCTCCACTTT GATCTCCAGA CCTCATCCCA CTGTGGGCGA 
     5821 ATCCCTGGCC TCTATCCCTC AACCCGTGGC AGCAGTCCAC AGCACTGGCC TTCAGGCAGG 
     5881 GAGCAGGAGC ACAGTGCCAC AACGTGTCAC CTTGTTCCGA CAGATGTCCT CGGGAGCCAT 
     5941 CCCCCCCAAC CGAGGAGTGC CTCCAGCACC CCCTCCACCA GCAGCTGTGC AGAGAGAGTC 
     6001 TCCCTCAGTC CTAAATACAG ACCCAGATGC AGAAAAACCC CGTTTTGCTT CGAATTTAAC 
     6061 GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT 
     6121 CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC TGTACAAGTA 
     6181 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6241 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6301 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6361 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6421 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6481 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6541 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     6601 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     6661 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     6721 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     6781 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     6841 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     6901 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     6961 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     7021 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     7081 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     7141 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     7201 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7261 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7321 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7381 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7441 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7501 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7561 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     7621 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     7681 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     7741 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     7801 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     7861 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     7921 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     7981 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     8041 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     8101 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     8161 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     8221 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8281 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8341 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8401 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8461 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8521 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     8581 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     8641 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     8701 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     8761 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     8821 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     8881 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     8941 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     9001 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9061 CAAATAGGGG TCCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//