LOCUS       MR221536                7968 bp    DNA     circular  SYN 
DEFINITION  Ogdhl (Myc-DDK-tagged) - Mouse oxoglutarate dehydrogenase-like (Ogdhl), nuclear gene encoding mitochondrial protein
ACCESSION   NM_001081130
VERSION     NM_001081130.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4125..4216 
                     /label="Myc-DDK"  
     misc_feature    4246..4831 
                     /label="PolyA signal"  
     misc_feature    4303..4322 
                     /label="Restriction Enzyme"  
     misc_feature    5107..5779 
                     /label="ColE1"  
     misc_feature    6209..7003 
                     /label="Kan/Neo"  
     misc_feature    7038..7340 
                     /label="SV40 ori"  
     misc_feature    7426..7903 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4107..4113 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4107 
                     /label="Ogdhl"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCAGCTG AGGCTGCTGC CATTCCGGCT GGGGCCACGG GCCACCAAGC 
     1081 TCCTGGCCAC ACGGGCTATC CCGGTGTTCA GTGGGTGCAG GAGGTCTTCT GGACCACCAA 
     1141 CGACCATCCC GAGGAGCAGA AGCGGAGTCA GCTCCAGTTA TGTGGAAGAG ATGTACTTTG 
     1201 CCTGGTTGGA AAACCCACAG AGCGTTCACA AGTCCTGGGA CAGCTTTTTT CAGAGAGCAA 
     1261 GTAAGGAGGC CTCTGTGGGT CCTGCTCAGC CGCAGCTCCC AGCTGTTCTC CAAGAAAGCA 
     1321 GGACATCAGT GTCAAGCTGC ACCAAGACCA GCAAGCTGGT GGAAGACCAC TTGGCAGTCC 
     1381 AGTCCCTGAT CCGGGCCTAC CAGATCCGAG GCCACCATGT GGCCCAGCTG GACCCCCTGG 
     1441 GCATCCTGGA TGCAGACCTA GATTCCTTTG TGCCCTCTGA CTTGATCACA ACCATTGATA 
     1501 AATTGGGTAA GAGTTGGGAT CCTTCCCTTC TTTCTTTCTA TGCTGCCTTG GCCTCTTTTC 
     1561 CAGCCTTCTA TGACCTGCAG GAGGCTGATC TGGATAAGGA GTTCCGGCTG CCCACCACGA 
     1621 CTTTCATCGG GGGCCCAGAG AACACACTCT CTCTTCGAGA GATCATACGT CGCTTGGAGA 
     1681 GCACCTACTG CCAGCATATT GGCCTGGAGT TCATGTTTAT TAATGACGTG GAACAGTGCC 
     1741 AGTGGATCAG ACAGAAATTT GAAACTCCTG GCGTGATGCA GTTTTCCGTC GAGGAGAAGA 
     1801 GGACCCTGCT GGCTAGACTG GTGCGCTCCA TGAGGTTTGA AGACTTCCTG GCCAGGAAGT 
     1861 GGTCTTCGGA GAAGCGGTTT GGCCTGGAAG GCTGCGAGGT CATGATTCCT GCTCTCAAGA 
     1921 CCATTATCGA CAAGTCTAGT GAGATGGGGA TTGAGAACGT CATCCTGGGG ATGCCACACA 
     1981 GGGGCAGGCT GAATGTGCTG GCTAACGTGA TCCGCAAGGA CCTGGAACAG ATCTTCTGTC 
     2041 AGTTTGATCC CAAGCTGGAA GCAGCAGATG AGGGCTCTGG TGATGTCAAA TACCATCTGG 
     2101 GCATGTACCA CGAGAGGATC AACCGTGTCA CCAACAGGAA CATCACATTG TCACTGGTAG 
     2161 CCAACCCTTC CCATCTAGAA GCTGTGGACC CCGTGGTACA GGGCAAGACG AAGGCAGAGC 
     2221 AGTTCTACCG TGGGGATGCC CAGGGTAGGA AGGTCATGTC TATCCTGGTC CATGGGGATG 
     2281 CCGCCTTCGC TGGCCAGGGG GTCGTCTATG AGACCTTCCA TCTCAGTGAC CTGCCTTCTT 
     2341 ATACCACCAA TGGCACAGTG CACGTTGTGG TCAACAACCA GATCGGCTTC ACCACAGATC 
     2401 CCCGAATGGC TCGCTCCTCC CCTTACCCCA CTGATGTGGC GCGGGTGGTC AATGCACCCA 
     2461 TCTTCCATGT GAACGCAGAC GACCCAGAGG CTGTGATCTA TGTGTGCAGT GTGGCAGCTG 
     2521 AGTGGAGGAA CACCTTCAAC AAAGATGTTG TTGTAGACCT GGTCTGTTAC CGCCGGCGTG 
     2581 GTCACAATGA GATGGACGAG CCCATGTTCA CGCAGCCACT CATGTACAAG CAGATCCACA 
     2641 AGCAGGTACC CGTGCTGAAG AAGTATGCAG ACAAGCTCAT CGCCGAGGGC ACCGTCACTC 
     2701 TGCAGGAGTT TGAGGAGGAA ATTGCCAAAT ATGACCGTAT CTGTGAGGAG GCCTACGGCA 
     2761 GGTCCAAGGA TAAGAAGATC CTGCATATAA AGCACTGGCT GGACTCCCCC TGGCCTGGCT 
     2821 TCTTCAATGT GGATGGGGAG CCCAAGAGCA TGACATGCCC TACGACAGGC ATCCCTGAGG 
     2881 AGATGCTGAC CCACATTGGC AGTGTGGCCA GCTCTGTGCC CCTGGAGGAC TTTAAGATCC 
     2941 ACACAGGCCT CTCTCGCATC CTGCGTGGCC GTGCAGACAT GACAAAGAAG CGCACAGTGG 
     3001 ACTGGGCCCT GGCAGAGTAC ATGGCCTTTG GTTCATTGCT GAAGGAGGGC ATCCACGTGC 
     3061 GACTCAGCGG CCAGGATGTG GAGAGGGGCA CATTCAGCCA CCGGCACCAT GTTCTTCATG 
     3121 ACCAGGAAGT TGACCGGAGG ACGTGTGTCC CGATGAATCA TCTGTGGCCT GACCAGGCCC 
     3181 CGTACACTGT GTGCAATAGC TCCCTCTCAG AGTATGGAGT TCTAGGCTTT GAGCTGGGCT 
     3241 ATGCCATGGC CAGCCCCAAC GCCCTGGTCC TCTGGGAAGC TCAGTTTGGT GATTTCCACA 
     3301 ACACAGCCCA GTGTATCATC GACCAGTTCA TCAGCACTGG CCAGGCCAAG TGGGTGCGGC 
     3361 ACAATGGCAT CGTGCTTCTG CTGCCCCACG GCATGGAGGG CATGGGTCCA GAGCACTCCT 
     3421 CAGCAAGGCC AGAGAGGTTC CTGCAGATGA GCAATGACGA CTCGGATGCC TACCCAGTGT 
     3481 TCACTGAGGA CTTTGAGGTG AGCCAGCTCT ACGACTGCAA CTGGATTGTG GTGAACTGCT 
     3541 CCACCCCAGC CAGCTACTTC CACGTGCTGC GCCGACAGAT CCTGCTGCCC TTCCGCAAGC 
     3601 CTCTCATTGT CTTCACACCC AAGTCTCTGC TGAGGCACCC TGATGCCAAG TCCAGCTTTG 
     3661 ACCAGATGGT GTCCGGAACT AGCTTCCAGC GGCTGATTCC GGAAGACGGC CCCGCAGCAC 
     3721 ATTCTCCTGA GCAGGTCCAG CGGCTCATCT TCTGCACGGG AAAGGTGTAC TATGACCTGG 
     3781 TAAAGGAACG CAGCAGCCAG GGCCTGGAGC AACAAGTGGC CATCACACGC CTGGAGCAGA 
     3841 TCTCTCCGTT TCCTTTCGAC CTGATCATGC GGGAGGCAGA GAAGTACTCG GGAGCTGAGC 
     3901 TGGTGTGGTG TCAGGAGGAA CATAAGAACA TGGGCTATTA CGACTACATC AGTCCACGCT 
     3961 TCATGACTCT CCTCGGCCAC TCCCGGCCCA TATGGTATGT CGGCCGGGAC CCAGCAGCCG 
     4021 CACCAGCCAC TGGGAATAAG AACGCTCACT TGGTGTCACT GAGGAGGTTT CTGGATACTG 
     4081 CCTTCAATCT GAAGGCCTTC GAGGGCAAGA CATTTACGCG TACGCGGCCG CTCGAGCAGA 
     4141 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4201 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4261 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4321 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4381 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4441 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4501 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4561 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4621 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4681 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4741 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4801 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4861 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4921 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4981 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5041 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5101 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5161 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5221 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5281 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5341 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5401 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5461 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5521 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5581 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5641 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5701 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5761 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5821 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5881 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5941 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6001 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6061 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6121 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6181 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6241 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6301 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6361 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6421 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6481 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6541 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6601 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6661 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6721 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6781 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6841 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6901 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6961 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7021 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7081 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7141 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7201 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7261 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7321 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7381 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7441 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7501 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7561 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7621 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7681 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7741 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7801 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7861 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7921 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//