LOCUS       MR221480                7806 bp    DNA     circular  SYN 
DEFINITION  Pigg (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class G (Pigg). Note: ORF is codon optimized
ACCESSION   NM_001081234
VERSION     NM_001081234.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3963..4054 
                     /label="Myc-DDK"  
     misc_feature    4084..4669 
                     /label="PolyA signal"  
     misc_feature    4141..4160 
                     /label="Restriction Enzyme"  
     misc_feature    4945..5617 
                     /label="ColE1"  
     misc_feature    6047..6841 
                     /label="Kan/Neo"  
     misc_feature    6876..7178 
                     /label="SV40 ori"  
     misc_feature    7264..7741 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3945..3951 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3945 
                     /label="Pigg"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGACTTGGG AGTGGGGCTT TTGCTGCGAG CTGCGTCGCG ATCGAGGTTA 
     1081 TCGGTGTTGC TATCTTCATT CGGGGCTTCT TCCCCGCTCC TGTTAGATCT AGTGCTCGAC 
     1141 CTGAGCACGA CGCGGAAACA CCTGCGCCAG AGCCCGTTGC TGGTGTAAGG TCCAATTGGA 
     1201 CTAAACTGCC TCCCCCACTG TTTTCCAAGG TTGTGATCGT TTTGATTGAC GCCCTGCGCG 
     1261 ACGACTTCGT GTTCGGGTCC AAGGGCGTGA AATATATGCC CTACACAACG TACTTGGTGG 
     1321 AGAAAGGAGC ATCACATAGC TTCGTGGCAG AGGCTAAGCC TCCTACGGTG ACAATGCCTA 
     1381 GGATTAAAGC CCTGATGACT GGCTCCCTGC CTGGCTTTGT TGACGTGATT AGGAACCTCA 
     1441 ATTCTCCAGT GCTGTTGGAA GACAACGTGC TGAGGCAAGC TAAGGCCGCC GGTAAGAGAA 
     1501 TCATATTCTA TGGCGATGAG ACATGGGTTA AACTGTTCCC CAAACATTTC GTTGAATACG 
     1561 ATGGCACAAC CTCTTTTTTC GTGTCAGACT ATATCGAAGT AGACAAGAAC GTGACTCGGC 
     1621 ACCTGGACAA AGTGCTCAAA AGGGGAGATT GGGACGTGCT CATCTTGCAT TACCTGGGCC 
     1681 TTGACCACAT TGGGCACATC TCAGGGCCCA ACAGCCCCCT CATCGGGCAT AAGCTTTCTG 
     1741 AGATGGACTC CGTACTCATG AAGATTCATA CCAGCCTGTT GTCCAAGGAT AGAGAGACAC 
     1801 TGCTCCCCTC TCTCCTGGTT CTCTGCGGAG ATCACGGCAT GTCCGAAACC GGTAGCCACG 
     1861 GCGCTTCCTC CACAGAGGAA GTTTCCACTC CCCTGCTGCT CATCAGCTCC GCCTTTGAGC 
     1921 GAAAGCCAGG GGACATTAGA CATCCTAAAC ATGTGCAACA GACAGACCTC GCCGCGACCT 
     1981 TGGCCATCGG TCTGGGCCTC CCCATACCCA AGGACTCTGT AGGGTCACTG CTGTTTCCTG 
     2041 TGATCGAGGG CAAGCCCATG AGGGAGCAGC TTCGATTCCT CCACCTCAAC ACGCTGCAAT 
     2101 TGTCCAAACT GCTGCAGGAA AACGTGCCTT CATACGAGAA AGACCCAGGT TTTGAGCAGT 
     2161 TTAAGATGGC TGAGAAACTG CATGGGAACT GGGTCAAGCT GCATCTGGAG GAGAACCACA 
     2221 GTGACATCCT GCTGGGACTC GGAACCAAAG TGTTGCGGCA CTATCTCGGA GCCCTCAAAA 
     2281 CTCTTTCCCT GTCATTGAGC ACGCAGGTGG CCCAATACGA TATGTACTCC ATGGCGGTTC 
     2341 TGGCACTTTT CTTGTTGAGC ACACCACACG TCCTGTGTAG AAAGGCTGAG CTCGACGTAC 
     2401 CGCTGCTGTC TCCCGTATTC TCCTTGCTGT TCTACCTGGT ATTCCTCGTT TTGTCTGCAA 
     2461 TCCATGTACT GGTCTGCACA AGCTCTGAGA GCAGTTGTTA CCTGTGTAGC CTGTCTTGGC 
     2521 TGGCCGTGGG AGCTGTCATG TTGCTGGTAA GTGCACTGTT CTGTGCAATT TTGTCCGCCC 
     2581 TGATCAGAAT GGTGATTGAC AGCACACTGC TCAAGAAGAA CGCCGCAGAC GCCAGCTCTG 
     2641 GTTGGTCCGA GGTGAATCTT TTGCTGCTGC TGGGCACTGT GGGACACGTA CTGAGCCTGG 
     2701 GGGCATCCTC CTTTGTCGAA GAAGAACATC AAACGTGGTA TTTCCTGATC AATACACTGT 
     2761 GCCTGGCGTT GAGTCAGGAG ACATGCCGGA GTTACTTTCT CGGCGATGAG TGCGAGCCAC 
     2821 AAAGACACAG CCACGTGGAG CAGCAGTGTG TCAACCTTCT GGCTTGCCCC CTGCAAGACT 
     2881 CTACTTCTTA TAATACCCCT GAGAGCGGAA CAGCTGGAAA GCGGGTTAGC CTCCTGGAGG 
     2941 CCCAGGGATC ATGCAAGTGG TGGACCGTCC TGGCCAGCCC GTGGCTGGTC CTGCTGTGTT 
     3001 GCCGCCTGCT GAGAAGCCTG AACCAGACGG GAGTGCAAGG CGCCCATCGA CCAGACTTCT 
     3061 CTCATTGGTT GACCAGCTCC GACCACAAGG TGCAACTTAG CGGGCTTGCC GCTCTGTCCC 
     3121 TGGTGGTGAT TTTCATGCTG GTGCAGAGGC GCTGTTCTCT GGTGAGTAAA GTGGCACTTG 
     3181 CCCTGGGACT TCTGGGGGTG TTCTGCTATA GAGCCGCAAT CGGTATAGTT CAATTCCCTT 
     3241 GGCAGTCTGA CAACAAGGGC ATCTCCAAGG GAATCATTGA GGCCAGGTTC GTATATGTCT 
     3301 TTGTGTTGGG GATTCTCTTC ACTGGTACTA AGGACCTGCT GAAGGCTCAG GTTATCGCCA 
     3361 CCGACTTCAA GACCAAAACC GTTGGACTCT GGGAGATGCA TTCTGGATTG GTCCTCCTCG 
     3421 CGGCCCTTCT GTTGCGGCCT CACAATCTGC CAGTTCTTGC TTTTAGCCTG CTGATTCAGA 
     3481 CCGTTATGAC CAAATTCATC TGGAAGCCTC TGCGGCACGA CGCAGCCGAA ATTACCGTGA 
     3541 TGCACTATTG GTTCGGGCAG GCCTTCTTTT ACTTCCAGGG AAATTCCAAC AACATAGCGA 
     3601 CTATCGACAT AAGCGCTGGT TTCGTGGGGC TCGATACATA CATGGAAGTC CCTGCTACCT 
     3661 TTTTGACGGT GTTCGGTACT TACGTTGGCC CTGTGCTGTG GGCAAGCCAC CTGGTCTATT 
     3721 TCCTTTCTTC CGAGGGGAAC AACTCAGCTC TGTCTCGGAG TTGCTTCTGT TACGCCCTGA 
     3781 TCTGTAGCGT GCCCGTTGCG ACATACATCG TCCTGGTCAC TAGCCTCCGC TATCATCTGT 
     3841 TTATTTGGTC TGTTTTCTCT CCCAAACTGC TGTACGAGGG TATGCATCTG CTGATAACTG 
     3901 CCGCCATCTG TGCAGTTTTC ACCGCCACAA ACCAGACCCG ACACCGAAGA GCAACGCGTA 
     3961 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4021 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4081 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4141 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4201 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4261 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4321 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4381 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4441 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4501 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4561 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4621 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4681 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4741 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4801 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4861 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4921 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4981 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5041 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5101 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5161 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5221 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5281 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5341 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5401 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5461 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5521 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5581 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5641 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5701 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5761 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5821 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5881 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5941 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6001 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6061 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6121 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6181 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6241 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6301 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6361 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6421 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6481 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6541 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6601 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6661 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6721 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6781 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6841 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6901 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6961 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7021 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7081 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7141 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7201 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7261 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7321 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7381 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7441 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7501 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7561 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7621 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7681 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7741 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7801 AATTTT
//