LOCUS       MR221408                8442 bp    DNA     circular  SYN 
DEFINITION  Ppfia4 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (Ppfia4)
ACCESSION   NM_001144855
VERSION     NM_001144855.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4599..4690 
                     /label="Myc-DDK"  
     misc_feature    4720..5305 
                     /label="PolyA signal"  
     misc_feature    4777..4796 
                     /label="Restriction Enzyme"  
     misc_feature    5581..6253 
                     /label="ColE1"  
     misc_feature    6683..7477 
                     /label="Kan/Neo"  
     misc_feature    7512..7814 
                     /label="SV40 ori"  
     misc_feature    7900..8377 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4581..4587 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4581 
                     /label="Ppfia4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTGAGGTG ATGCCCACAA TCAATGAGGG GGACCCCCTG GGGCCCCCCC 
     1081 ACGGTGCCGA TGCTGAGGCC AACTTTGAGC AGCTGATGGT GAACATGCTG GATGAGCGAG 
     1141 AGAAGTTGCT GGAGTCCCTT CGGGAGAGTC AGGAAACTTT GGTAGCCACA CAGAGCCGGC 
     1201 TCCAGGATGC CCTTCACGAG CGAGATCAGC TCCAGCGCCA CCTGAACTCC GCCCTTCCCC 
     1261 AGGAATTTGC CACTTTAACT CGGGAGCTAA GCATGTGTCG GGAGCAGCTT CTGGAGCGGG 
     1321 AGGAAGAGAT ATCTGAGCTG AAAGCAGAGC GGAATAACAC AAGGCTGCTT CTGGAACATC 
     1381 TGGAATGCCT CGTGTCCCGC CATGAGCGGT CATTGCGGAT GACAGTGGTA AAACGGCAGG 
     1441 CCCAATCACC TTCAGGTGTC TCCAGTGAGG TGGAGGTGCT GAAGGCCCTC AAGTCACTAT 
     1501 TTGAGCACCA CAAGGCCCTG GATGAGAAGG TGCGAGAGCG GCTCCGGGCA GCTCTAGAGC 
     1561 GCGTTACTAC CTTGGAGGAG CAGTTGGCAG GAGCTCACCA GCAGGTATCT GCCCTGCAGC 
     1621 AGGGTGCAGG AATTCGGGAT GGAGTAGCAG AAGAGGAAGG GACTGTGGAT CTGGGACCAA 
     1681 AACGCCTGTG GAAAGATGAC ACTGGCCGCG TGGAAGAGCT GCAAGGACTC TTGGAGAAGC 
     1741 AAAACTATGA GTTGAGCCAG GCCCGTGAGC GGCTGGTCAC CCTGAGTGCC ACCGTGACTG 
     1801 AGCTGGAGGA GGACCTGGGC ACGGCCCGCC GAGACCTCAT CAAGTCAGAG GAGCTGAGCA 
     1861 GCAAGCATCA GCGGGACCTC CGAGAGGCTC TGGCTCAGAA GGAGGACATG GAAGAGAGAA 
     1921 TCACCACACT GGAGAAGCGC TACTTGGCTG CTCAGCGAGA AGCCACATCC ATCCATGACC 
     1981 TCAATGACAA ACTGGAGAAT GAGCTGGCCA ACAAGGAATC CCTGCACCGC CAGTGTGAGG 
     2041 AGAAAGCCCG GCATCTGCAG GAGCTGCTAG AAGTAGCAGA GCAGAAGTTG CAGCAGACCA 
     2101 TGCGCAAGGC AGAGACTCTG CCCGAGGTGG AGGCTGAGCT GTCTCAGAGA ATCGCAGCCC 
     2161 TCACCAAGGC TGAGGAACGT CATGGCAACA TTGAGGAACA TCTGCGGCAG CTGGAAGGGC 
     2221 AGCTGGAAGA GAAGAACCAG GAGCTGGCAA GGGTGCGCCA GCGGGAGAAG ATGAATGAAG 
     2281 ACCACAACAA GCGGCTGTCA GACACCGTGG ACCGGTTGCT TAGTGAGTCC AACGAACGCC 
     2341 TGCAGCTCCA CCTCAAGGAG CGAATGGCTG CCCTGGAGGA GAAGGGCCGT CTGTCTGAAG 
     2401 AGATTGAGAA GCTGCGCCAA GAGGTGGACC AGCTGAAGGG TCGAGGGGGG CCGTTTGTGG 
     2461 ATGGCATCCA CTCCAGGTCA CACGTGGGCA GCGCAGCAGA TGTGAGGTTC TCACTGAGTA 
     2521 CAGCCACACA CGCGCCCCCA GGTCTGCATC GACGCTATTC GGCGCTGAGG GATGAATCTG 
     2581 CCAAGGACTG GAAGCCATCG CCACTGCCAG GGGTGTTGGG CGCCACCACG CCTGCCTTTG 
     2641 ACAGTGACCC TGAGATCTCT GATGTGGATG AGGATGAGCC TGGGGGCTTG GTGGGCACTC 
     2701 AGGTCGATGT CATCTCACCC GGTGGCCATT CAGATGCCCA GACCCTGGCC ATGATGCTAC 
     2761 AGGAGCAGCT GGATGCCATC AACCAGGAGA TCAGGATGAT CCAGGAGGAG AAGGAGTCCA 
     2821 CTGAGCTTCG AGCTGAGGAG ATTGAGACCC GTGTGACCAG CGGCAGCATG GAGGCCCTCA 
     2881 ACCTGACCCA GCTGCGCAAG CGTGGCTCTA TCCCCACCTC TCTGACCGCC CTTTCCCTGG 
     2941 CCAGTGCATC TCCACCGCTC AGTGGCCGCT CCACACCCAA GCTCACTTCC CGAAGTGCTG 
     3001 CCCAGGATCT GGACCGAATG GGGGTCATGA CCCTGCCCAG CGACTTAAGA AAGCATAGGA 
     3061 GGAAGCTGCT GTCGCCAGTG TCTCGGGAAG AGAACCGAGA GGATAAAGCC ACCATAAAAT 
     3121 GTGAGACTTC TCCTCCTTCC TCACCCAGGA CGCTGCGGCT AGAGAAGCTT GGCCACCCTG 
     3181 GCCTGAGTCA AGAGGAGGGC AAGAGTGCCT TGGAGGGTCA GGACAGCAAC CCCAGCAGCA 
     3241 GCAACAGCAG CCAGGACTCC CTGCACAAGG GCGCCAAGCG CAAGGGCATC AAGTCATCCA 
     3301 TTGGCCGCCT GTTTGGGAAG AAGGAGAAGG GCAGGCTGAT CCAGCTGAGT CGGGATGCCA 
     3361 CGGGCCATGT TCTGCTCACA GACTCCGAGC TCAGTCTGCA GGAACCAATG GTACCTGCTA 
     3421 AGTTGGGGAC CCAGGCAGAG AAGGACCGGC GACTGAAAAA GAAACACCAG CTGCTTGAAG 
     3481 ATGCGCGCCG AAAAGGAATG CCCTTTGCCC AATGGGATGG CCCCACGGTG GTCTCCTGGC 
     3541 TGGAGCTCTG GGTGGGGATG CCTGCCTGGT ATGTGGCGGC CTGCCGGGCC AATGTCAAGA 
     3601 GTGGCGCCAT CATGTCCGCC TTGTCGGACA CGGAGATCCA GCGGGAGATC GGCATCAGCA 
     3661 ATGCCCTGCA CAGGCTCAAG CTCCGCCTGG CCATCCAGGA GATGGTGTCA CTGACGAGCC 
     3721 CCTCAGCTCC ACCTACCTCC AGGACTTCTT CAGGGAATGT CTGGGTGACC CACGAAGAGA 
     3781 TGGAAACTCT GGCAACATCT ACCAAAACAG ACAGTGAGGA GGGCAGCTGG GCCCAGACCC 
     3841 TGGCCTATGG AGACATGAAT CACGAGTGGA TTGGAAACGA ATGGCTGCCC AGCCTGGGGC 
     3901 TCCCTCAGTA CCGTAGTTAC TTCATGGAGT GCCTGGTGGA TGCCCGCATG CTGGATCACC 
     3961 TCACCAAGAA GGACCTGCGT GTCCATCTCA AGATGGTGGA CAGTTTCCAT AGAACCAGTC 
     4021 TTCAGTATGG CATCATGTGT CTGAAGAGGC TGAATTATGA CCGGAAGGAA CTGGAAAAGA 
     4081 GGCGGGAAGA GAGCCAGCAT GAGATCAAGG ATGTGCTGGT CTGGACCAAT GACCAGGTGG 
     4141 TTCACTGGGT CCAGTCTATA GGGCTAAGGG ACTATGCAGG AAACCTACAC GAGAGTGGTG 
     4201 TGCATGGCGC CCTGCTGGCC CTGGATGAAA ACTTCGACCA CAACACACTG GCCTTGGTTC 
     4261 TGCAGATCCC CACACAGAGC ACCCAGGCCC GCCAAGTGAT GGAGAGAGAG TTCAATAACC 
     4321 TGTTGGCCTT GGGCACAGAC AGGAAATTGG ATGACGGAGA AGAGAAGGTG TTCCGCCGAG 
     4381 CACCCTCCTG GAGGAAACGC TTCCGGCCTC GGGATCACCA TAGCGGTGGC ATGTTGGGCA 
     4441 CCTCAGCGGA GACCCTCCCG GCGGGCTTCC GTGTGTCCAC GCTGGGTCCG CTGCAGCCTC 
     4501 CACCTGCCCC GCCTAAGAAG ATCATGCCGG AAGCCGGGAC GGCGGGGGCG CAGAGACTGG 
     4561 AGCCCTCTAC GGTCCGGACC TACTCCTGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4621 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4681 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4741 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4801 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4861 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4921 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4981 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5041 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5101 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5161 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5221 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5281 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5341 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5401 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5461 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5521 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5581 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5641 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5701 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5761 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5821 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5881 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5941 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6001 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6061 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6121 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6181 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6241 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6301 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6361 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6421 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6481 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6541 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6601 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6661 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6721 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6781 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6841 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6901 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6961 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7021 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7081 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7141 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7201 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7261 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7321 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7381 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7441 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7501 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7561 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7621 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7681 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7861 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7921 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7981 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8041 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8101 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8161 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8221 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8281 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8341 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8401 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//