LOCUS       MR221045                8427 bp    DNA     circular  SYN 
DEFINITION  Nlrp1a (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 1A (Nlrp1a)
ACCESSION   NM_001004142
VERSION     NM_001004142.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="Nlrp1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGAATCT CAGTCCAAGC AGGAAAGTAG CACAAAAGTG GCTCAGCATG 
     1081 AAGGTCAAGA GGATGTGGAC CCAACCTTCA AAACAAAGAA ACTTATGGAG GTGGAGCTAA 
     1141 TGAAGCACAG AGTGCAGCTA GAGAGGAACT TGAAGCTAAG AACCTTTCCA GGAGCACGTA 
     1201 CGAAGCAAGT AAAAGAGGCT CTTTACCCTC TTCTAACATG GTCATCAAAA AGCAAGAATC 
     1261 TGTTCCAAAA TTTCACAAAA CTACTACTCT TTAAAAAATT GTGCCAAAGA GGCTCAGAAA 
     1321 ACTTGGTCAG GGAAAGCTGG TATCCATGTG TACCAGAAGA AGAAGCACAT ATGATTGACA 
     1381 TCCAAGATTT ATTTGGCCCA AACTTAGGTA CTCAGAAAAA GCCTCAATTA GTCATAATAG 
     1441 AAGGAGCTGC TGGGATTGGG AAGTCAACAC TGGCCAGACT TGTGAAGAGA GCCTGGAAGG 
     1501 AAGGCAAGCT TTACAGGAAT GACTTCCATC ATGTCTTCTT CTTCAGCTGC AGAGAGCTGG 
     1561 CCCAGTATGA GCAGCTGAGT CTGGCTGAGC TCATAGTACA AGGCCAGGAA GTGCCCACAG 
     1621 CTCCCATTAG GCAGATCCTG TCTCACCCTG AGAAGTTGCT CTTCATTCTG GATGGCATAG 
     1681 ATGAGCCAGC ATGGGTCTTA GCAGACCAGA ATCCTGAGTT ATGTCTTCAC TGGAGTCAAA 
     1741 CCCAGCCTGT GCACACTCTG CTGGGCAGTT TACTTGGGAA ATCCATCCTT CCTGGGGCTT 
     1801 CCTTCTTGCT CACAACTCGC ACCACAGCTC TACAGAAATT CATTCCTTCC CTGGAGCAGC 
     1861 CATGTCAAGT GGAAGTCCTA GGATTCACTT TGTTTGAACG GAAAAACTAT TTCTACAAAT 
     1921 ATTTTGGGAA GAAAAAAGGT GGAGTTACTA CTTTTACATT AGTTAAATCA AACTCAGCGC 
     1981 TCCTGACTCT GTGTGAAGTT CCCTGGGTGT GCTGGTTGGT CTGCACTTGC CTGAAAAAGC 
     2041 AGATGGAGCA GGGTGGAGAG CTCTCACTGA CCTCACAGAC CACCACAGCA CTCTGCCTGA 
     2101 AATACCTTTC CCTGACGATC CCAGGGCAGC ACATGAGGAC CCAGCTCAGA GACCTCTGCT 
     2161 CACTGGCTGC TGAGGGGGTC TGCCAAAGAA GGACCCTGTT CAGTGAAAGC GATCTCTGTA 
     2221 AGCAAGGGTT AGATGAGCAT GCCATTGCCA GTTTCCTGAA GATTGGTGTC CTTCAAAAGC 
     2281 AGGCCAGCTC TCTGAGCTAC AGCTTTGCCC ACTTGTGTCT TCAGGAGTTC TTTGCAGCAA 
     2341 TGTCCTACAT CTTGGATGAT AGTGAGGAAA GACATGCTGA TATGAAAAAT GACAGAATTG 
     2401 TGGAAACACT GGTAGAAAGG TATGGAAGAC AAAACCTGTT TGAGGCACCC ACTGTGCGTT 
     2461 TTTTATTTGG TCTTTTGAGT AAAGAGGAAT TGAAAAAAAT AGAAAAGCTC TTTTCCTGCA 
     2521 GCCTTCATGG AAAGACAAAG TTGAAACTAC TATGGCACAT CCTAGGGAAA TCCCAACCCC 
     2581 ATCAACCACC TTGCCTGGGT TTGCTCCACT GTCTGTATGA AAATCAGGAT ATGGAGCTCC 
     2641 TGACACACGT GATGCATGAT CTGCAAGGAA CAATAGTGCC TGGACCAGAT GATTTGGCAC 
     2701 ACACAGTGTT GCAGACAAAC GTGAAGCACC TGGTGATACA GACAGACATG GACCTCATGG 
     2761 TGGTTACTTT CTGCATTAAG TTCTGCTGTC ACGTGAGGAG TCTTCAGCTG AACAGGAAGG 
     2821 TACAGCAGGG ACATAAGTTT ACGGCCCCTG GGATGGTTCT GTACAGATGG ACCCCAATCA 
     2881 CTGATGCCAG CTGGAAGATT TTCTTCTCCA ATCTTAAGTT AGCCAGAAAC CTGGAGGAGT 
     2941 TGGACCTAAG TGGAAATCCA CTGAGCTACT ATGCAGTACA CAGTCTTTGT ACGACCCTGA 
     3001 GAAAGCGTGG CTGCCAACTA AAGACTCTGT GGCTTGTTGA ATGCGGCCTC ACATCCACAT 
     3061 ACTGCTCACT TCTGGCCTCA GTACTCAGTG CCCGCTCCAG CCTGACTGAG CTAGACCTGC 
     3121 AGCTGAATGA CCTGGGTGAT GGTGGTGTGA AGATGTTGTG TGAGGGGCTC AGGAATCCTG 
     3181 CCTGCAATCT CAGCATCCTG TGGCTGGACC AGGCATCTCT CAGTGACCAA GTGATTGCAG 
     3241 AGCTCAGAAC TCTGGAGGCA AAGAATCCAA AGCTGCTCAT CTCCAGCACA TGGAAGCCAC 
     3301 ATGTGATGGT CCCTACGATG AACATGGATA AAGAAGAAGT GGGTGATAGC CAGGCCCTAC 
     3361 TGAAGCAGCA GAGACAGCAG TCAGGAGACA AACACATGGA ACCTCTGGGG ACTGAAGATG 
     3421 AGTTCTGGGG CCCTACAGGA CCTGTGACTA CTGAGGTGGT TGACAGAGAG AGGAACCTGT 
     3481 ACCGAGTTCA GTTACCCATG GCTGGTTCCT ACCACTGTCC CAGTACAGGA CTCCACTTTG 
     3541 TGGTGACAAG GGCAGTGACA ATCGAGATTG AATTCTGTGC CTGGAGCCAA TATCTCGACA 
     3601 AGACTCCCTT GCAGCAGAGT CACATGGTGG TTGGACCTCT GTTTGACATC AAGGCTGAGC 
     3661 AAGGAGCTGT GACTGCAGTG TACCTCCCTC ATTTTGTGGC CCTCCAAGAG GGAATCGTGG 
     3721 ACAGCTCCTT GTTCCATGTT GCCCACTTTC AAGAACATGG GATGGTCCTA GAAACACCAG 
     3781 CAAGGGTGGA ACAGCATTAC GCAGTACTGG AAAACCCAAG CTTCTCCCCA ATGGGGATTC 
     3841 TACTGAGAAT GATTCCGGCT GTCGGACACT TCATCCCCAT CACTTCCACC ACACTGATCT 
     3901 ACTATCACCT CTATCTTGAA GACGTCACCT TTCACCTTTA CCTGGTCCCC AATGACTGCA 
     3961 GTATTCGAAA GGCCATTGAT GATGAAGAAA TGAAGTTTCA GTTTGTGCGC ATAAACAAGC 
     4021 CACCCCCAGT AGACGCTCTT TATCTTGGTT CCCGCTATAT CGTGTCTTCC TCCAAATTGG 
     4081 TGGAAATTAT CCCAAAGGAA CTAGAGCTGT GCTACCGAAG CCCTGGGGAA TCCCAGCTCT 
     4141 TCTCTGAGAT CGACATTGGG CACATGGATT CAGAGATTAA GCTGCAAATC AAAGACAAGA 
     4201 GGCACATGAA TCTCAAATGG GAGGCCTTGC TGAAACCAGG GGATCTCAGA CCTGCACTGC 
     4261 CCAAGATTGC TACAGCCCCC AAAGATGCTC CTTCCTTGCT GCACTTCATG GACCAGCATC 
     4321 GGGAGCAGCT GGTGGCCCGA GTGACATCTG TAGATCCTCT CTTGGACAAG CTGCATGGTC 
     4381 TGGTGCTGAG TGAAGATTCC TATGAAGTAG TGCGGTCTGA GACCACAAAC CAAGACAAGA 
     4441 TGAGGAAGCT CTTCAGCCTC AGCCGGTCCT GGAGCTGGGA TTGCAAAGAC CAATTCTACC 
     4501 AAGCTCTGAA GGAGACCCAT CCTCACCTGG TCATGGATAT ACTTGAGAAG TTGGGTGGGG 
     4561 TCTCTGTGAA ATCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//