LOCUS       MR220972                8859 bp    DNA     circular  SYN 
DEFINITION  Grin2d (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d)
ACCESSION   NM_008172
VERSION     NM_008172.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5014..5105 
                     /label="Myc-DDK"  
     misc_feature    5135..5720 
                     /label="PolyA signal"  
     misc_feature    5192..5211 
                     /label="Restriction Enzyme"  
     misc_feature    5996..6668 
                     /label="ColE1"  
     misc_feature    7098..7892 
                     /label="Kan/Neo"  
     misc_feature    7927..8229 
                     /label="SV40 ori"  
     misc_feature    8315..8792 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4996 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="Grin2d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCGGCGCC GGTGGCCCCC GCGGCCCTCG GGGCCCCGCT AAGATGCTGT 
     1081 TGCTGCTGGC GCTGGCGTGC GCCAGCCCGT TCCCGGAGGA GGTGCCGGGG CCGGGCGCGG 
     1141 CCGGCGGGGG CACGGGCGGG GCGCGGCCGC TCAACGTGGC GCTGGTCTTC TCTGGCCCGG 
     1201 CGTACGCGGC CGAGGCGGCG CGCTTGGGCC CGGCCGTGGC GGCGGCAGTG CGCAGCCCGG 
     1261 GCCTGGACGT ACGGCCCGTG GCGCTGGTGC TCAACGGCTC CGACCCTCGC AGCCTTGTGC 
     1321 TGCAGCTCTG CGACCTGCTG TCGGGGCTGC GCGTGCACGG CGTGGTGTTC GAGGACGACT 
     1381 CGCGCGCGCC CGCCGTCGCG CCCATTCTCG ACTTCCTGTC GGCGCAGACC TCGCTGCCCA 
     1441 TCGTGGCCGT GCACGGCGGC GCCGCGCTCG TACTCACACC CAAGGAGAAG GGCTCCACCT 
     1501 TCCTGCAGCT TGGCTCCTCC ACAGAGCAAC AGCTGCAGGT CATTTTTGAG GTGCTGGAGG 
     1561 AGTACGACTG GACATCCTTT GTGGCAGTGA CTACGCGTGC CCCAGGCCAT CGAGCCTTCT 
     1621 TGTCATACAT CGAGGTGCTG ACTGATGGCA GCCTGGTGGG CTGGGAGCAT CGAGGAGCGC 
     1681 TGACACTGGA CCCCGGAGCG GGTGAGGCCG TCCTGGGCGC ACAGCTCCGT AGTGTCAGTG 
     1741 CGCAGATCCG CCTGCTCTTC TGCGCCCGCG AGGAGGCAGA GCCTGTTTTC CGGGCGGCAG 
     1801 AAGAGGCTGG TCTCACTGGG CCTGGCTACG TCTGGTTCAT GGTGGGACCT CAGCTGGCCG 
     1861 GAGGTGGGGG CTCCGGGGTC CCTGGGGAAC CACTTCTTCT GCCAGGAGGT GCCCCACTGC 
     1921 CTGCTGGGCT GTTTGCAGTG CGCTCTGCTG GCTGGCGTGA CGACTTGGCA CGTCGAGTGG 
     1981 CTGCTGGTGT GGCGGTGGTG GCCAGAGGTG CCCAGGCCCT GCTGCGAGAC TATGGCTTCC 
     2041 TGCCTGAGCT GGGCCATGAC TGTCGCGCCC AGAATCGCAC CCACCGCGGG GAGAGTCTGC 
     2101 ACAGGTATTT CATGAACATC ACCTGGGATA ACCGAGACTA CTCCTTCAAT GAGGATGGCT 
     2161 TTCTGGTAAA CCCGTCACTG GTAGTCATCT CCCTCACCAG AGACAGGACG TGGGAAGTGG 
     2221 TGGGCAGCTG GGAACAGCAG ACCCTCCGCC TCAAGTACCC TCTATGGTCC CGCTATGGCC 
     2281 GCTTCCTGCA GCCGGTGGAT GACACGCAGC ACCTCACTGT GGCCACGCTG GAGGAGAGAC 
     2341 CTTTTGTCAT TGTAGAGCCT GCAGACCCCA TCAGCGGCAC TTGCATCAGA GACTCGGTTC 
     2401 CCTGCCGGAG CCAGCTCAAC CGTACCCACA GCCCTCCGCC TGACGCTCCC CGCCCGGAGA 
     2461 AGAGATGCTG CAAGGGTTTC TGCATTGACA TTTTGAAGAG GCTGGCGCAC ACCATCGGTT 
     2521 TCAGCTATGA CCTCTACCTG GTTACCAACG GCAAGCATGG CAAGAAGATC GATGGCGTCT 
     2581 GGAATGGCAT GATTGGTGAG GTGTTCTATC AGCGTGCGGA CATGGCCATC GGCTCCCTCA 
     2641 CCATCAATGA GGAGCGGTCA GAGATCGTGG ACTTCTCCGT CCCTTTTGTA GAGACAGGCA 
     2701 TCAGCGTCAT GGTGGCACGC AGCAATGGCA CTGTGTCCCC CTCTGCCTTC CTCGAGCCCT 
     2761 ACAGCCCCGC TGTGTGGGTG ATGATGTTCG TCATGTGCCT CACCGTGGTC GCCGTCACAG 
     2821 TTTTCATCTT TGAGTACCTC AGTCCTGTGG GCTATAACCG AAGCCTGGCC ACGGGCAAAC 
     2881 GCCCCGGAGG CTCTACCTTC ACCATTGGGA AATCCATCTG GCTGCTGTGG GCCCTGGTGT 
     2941 TCAACAACTC CGTGCCAGTG GAGAATCCTC GGGGCACCAC CAGCAAGATC ATGGTGCTGG 
     3001 TGTGGGCCTT CTTTGCCGTC ATCTTTCTTG CCAGCTATAC AGCCAATCTG GCTGCCTTCA 
     3061 TGATCCAGGA GGAGTACGTG GACACCGTGT CTGGGCTCAG CGACCGGAAG TTCCAGCGGC 
     3121 CCCAGGAGCA ATACCCACCC CTGAAGTTTG GAACGGTGCC CAATGGGTCC ACGGAGAAGA 
     3181 ATATCCGAAG CAACTACCCT GATATGCACA GCTACATGGT GCGATACAAC CAGCCAAGAG 
     3241 TGGAGGAGGC GCTCACTCAG CTCAAGGCAG GGAAACTGGA CGCCTTCATC TATGACGCAG 
     3301 CAGTGCTCAA CTACATGGCC CGAAAGGATG AGGGCTGCAA GCTGGTCACC ATCGGCTCAG 
     3361 GCAAGGTCTT CGCCACCACT GGTTATGGCA TCGCCCTACA CAAGGGCTCC CGCTGGAAGA 
     3421 GGCCCATCGA CCTGGCGCTG CTGCAGTTCC TGGGGGACGA TGAGATTGAG ATGCTGGAGC 
     3481 GGCTGTGGCT TTCAGGGATC TGCCACAACG ACAAAATCGA GGTGATGAGC AGCAAGCTGG 
     3541 ATATCGACAA CATGGCAGGT GTCTTCTACA TGCTCCTCGT GGCCATGGGC CTCTCCTTGC 
     3601 TGGTCTTCGC CTGGGAACAC CTTGTGTACT GGCGACTGCG GCACTGTCTG GGGCCCACCC 
     3661 ACCGCATGGA TTTCCTACTG GCCTTCTCCA GGGGTATGTA CAGCTGCTGC AGCGCTGAGG 
     3721 CTGCTCCGCC GCCGGCCAAA CCCCCGCCAC CGCCGCAGCC GCTGCCCAGT CCGGCGTATC 
     3781 CCGCCGCTCG CCCACCCCCT GGCCCCGCAC CCTTCGTGCC CCGAGAGCGC GCAGCCGCCG 
     3841 ACCGCTGGCG CCGGGCCAAG GGCACAGGGC CCCCGGGGGG CGCAGCGCTA GCCGACGGCT 
     3901 TCCACCGATA CTACGGCCCC ATCGAGCCGC AGGGGCTGGG CCTCGGCGAG GCGCGCGCGG 
     3961 CACCGAGAGG CGCAGCCGGA CGCCCACTGT CCCCACCCAC CACACAGCCC CCACAGAAGC 
     4021 CACCACCTTC CTACTTCGCC ATCGTGCGCG AGCAAGAGCC GGCCGAGCCC CCCGCCGGCG 
     4081 CCTTCCCGGG CTTCCCATCT CCGCCCGCTC CGCCTGCCGC CGCAGCCGCC GCCGTCGGGC 
     4141 CGCCACTGTG CCGCCTGGCT TTCGAGGACG AGAGCCCGCC CGCGCCCTCG CGCTGGCCGC 
     4201 GTTCTGACCC CGAGAGCCAG CCGCTGTTGG GTGGGGGCGC GGGCGGCCCG AGCGCTGGGG 
     4261 CCCCGACCGC ACCACCGCCG CGCCGCGCCG CGCCACCACC GTGCGCCTAC CTGGACCTCG 
     4321 AGCCTTCGCC TTCGGACTCC GAGGATTCGG AGAGCCTGGG CGGAGCGTCG CTCGGTGGCC 
     4381 TGGAGCCCTG GTGGTTCGCC GACTTCCCCT ACCCGTATGC GGAGCGCCTC GGGCCGCCGC 
     4441 CCGGCCGCTA CTGGTCGGTT GACAAGCTCG GGGGCTGGCG CGCTGGTAGC TGGGACTACC 
     4501 TGCCCCCGCG CGGCGGCCCC GCATGGCACT GCCGCCACTG CGCCAGCCTG GAGCTGCTAC 
     4561 CGCCACCACG CCATCTCAGC TGCTCGCACG ACGGCCTAGA CGGTGGCTGG TGGGCGCCTC 
     4621 CGCCTCCACC CTGGGCTGCG GGGCCACCGC CCCGGCGCCG GGCGCGCTGT GGTTGTCCGC 
     4681 GACCGCACCC GCACCGCCCC CGGGCTTCGC ACCGTGCGCC CGCCGCCGCA CCGCACCACC 
     4741 ACCGACACAG GCGCGCGGCG GGTGGCTGGG ACCTCCCGCC GCCCGCGCCC ACCTCGCGTT 
     4801 CGCTGGAGGA CCTGAGCTCC TGCCCACGGG CGGCCCCCAC GCGCAGGCTC ACCGGGCCCT 
     4861 CGCGCCACGC GCGCCGCTGT CCGCACGCTG CGCATTGGGG GCCGCCCCTG CCCACCGCAT 
     4921 CTCACCGGAG ACACCGGGGC GGGGACCTGG GCACACGCAG GGGCTCTGCG CACTTCTCCA 
     4981 GCCTGGAGTC CGAGGTAAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5041 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5101 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5161 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5221 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5281 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5341 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5401 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5461 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5521 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5581 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5641 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5701 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5761 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5821 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5881 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5941 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6001 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6061 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6121 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6181 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6241 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6301 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6361 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6421 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6481 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6541 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6601 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6661 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6721 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6781 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6841 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6901 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6961 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7021 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7081 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7141 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7201 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7261 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7321 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7381 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7441 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7501 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7561 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7621 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7681 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7741 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7801 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7861 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7921 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7981 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8041 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8101 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8161 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8221 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8281 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8341 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8401 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8461 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8521 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8581 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8641 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8701 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8761 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8821 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//