LOCUS       MR220716                7860 bp    DNA     circular  SYN 
DEFINITION  Dsg3 (Myc-DDK-tagged) - Mouse desmoglein 3 (Dsg3)
ACCESSION   NM_030596
VERSION     NM_030596.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4017..4108 
                     /label="Myc-DDK"  
     misc_feature    4138..4723 
                     /label="PolyA signal"  
     misc_feature    4195..4214 
                     /label="Restriction Enzyme"  
     misc_feature    4999..5671 
                     /label="ColE1"  
     misc_feature    6101..6895 
                     /label="Kan/Neo"  
     misc_feature    6930..7232 
                     /label="SV40 ori"  
     misc_feature    7318..7795 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3999..4005 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3999 
                     /label="Dsg3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTGCCTC TTCCCTAGAG CCTTGGGGTC TCTGGCCCTC CTGATGGTGG 
     1081 TGCTGCTGGT ACAGGGGGAA CTGCATGTGA AGCCGGGAGG ACAGCACAGA GAAGATGGGA 
     1141 CAGCATTGCA GCTGGCCAAA AGAAGGTACA AACGTGAATG GGTGAAATTT GCAAAACCCT 
     1201 GCAGAGAAAG AGAAGACAAC TCAAGACGAA ACCCAATTGC CAAGATCACT TCAGATTTCC 
     1261 AAAAAAACCA GAAAATTACC TACCGCATTT CTGGAGTGGG AATTGATCAG CCTCCCTTTG 
     1321 GGATCTTTGT TGTTGACCCA AACAATGGTG ATATCAACAT AACAGCCATA GTTGATCGTG 
     1381 AGGAAACCCC AAGCTTCCTG ATCACATGCC GTGCACTAAA TGCCCTGGGA CAGGATGTAG 
     1441 AGAGACCACT TATATTAACA GTGAAAATTT TAGACGTCAA TGATAACCCT CCAATATTTT 
     1501 CACAAACCAT ATTCAAGGGT GAAATTGAAG AAAATAGTGC TTCAAATTCC CTGGTAATGA 
     1561 TCCTAAATGC CACGGATGCA GATGAACCAA ACCATATGAA CTCTAAAATT GCCTTCAAAA 
     1621 TTGTCTCCCA GGAGCCCGCA GGCATGTCCA TGTTCCTCAT CAGCAGAAAC ACCGGAGAAG 
     1681 TCCGCACTTT GACCAGTTCT CTTGATCGAG AGCAAATTAG TAGCTACCAT CTGGTTGTGA 
     1741 GCGGTGCAGA CAACGATGGA ACAGGACTGT CAACTCAGTG TGAATGTAGT ATTAAAATTA 
     1801 AAGATGTCAA TGACAACTTC CCAGTGCTCA GAGAGTCTCA GTATTCAGCA CGTATTGAAG 
     1861 AAAATACTTT GAATGCAGAG CTACTCCGAT TTCAAGTGAC AGACTGGGAT GAAGAATACA 
     1921 CAGATAATTG GTTGGCTGTG TATTTCTTCA CCTCTGGGAA TGAGGGGAAT TGGTTTGAAA 
     1981 TTGAAACAGA TCCTAGAACC AATGAAGGCA TCCTGAAGGT GGTTAAGGCT CTGGATTATG 
     2041 AACAAGTGCA GAGTATGCAG TTTAGTATTG CTGTCAGAAA CAAAGCTGAG TTCCACCAGT 
     2101 CAGTGATCTC TCAGTACCGA GTGCAGTCAA CGCCAGTCAC AATTCAAGTC ATTGATGTCC 
     2161 GAGAGGGAAT CTCATTCCGT CCTCCTTCCA AGACATTTAC TGTGCAAAGG GGTGTAAGCA 
     2221 CTAACAAATT AGTTGGTTAC ATCCTGGGGA CATACCAAGC TACTGATGAG GACACGGGTA 
     2281 AAGCTGCCTC CTCTGTCAGA TATGTCCTGG GTCGTAATGA TGGTGGTTTG CTTGTTATTG 
     2341 ATTCAAAGAC TGCTCAGATC AAATTTGTCA AAAACATCGA CCGAGATTCT ACTTTCATAG 
     2401 TTAACAAGAC AATTTCTGCT GAGGTTCTGG CCATAGACGA AAACACTGGT AAGACCTCCA 
     2461 CAGGCACAAT ATATGTGGAA GTGCCCAGTT TTAATGAAAA CTGCCCGTCA GTTGTCCTCG 
     2521 AGAAGAAAGA CATCTGTACT TCCTCACCTT CTGTGACCCT CTCAGTGAGA ACCCTGGACA 
     2581 GGGGTAAATA CACTGGCCCC TACACAGTGT CCCTGGAGGA GCAGCCACTG AAGCTGCCAG 
     2641 TCATGTGGAC CATCACGACG CTGAATGCTA CATCAGCTCT TCTACAAGCC CAGCAGCAAG 
     2701 TGTCTCCAGG AGTGTACAAT GTCCCAGTCA TCGTGAAGGA CAATCAGGAT GGGCTGTGTG 
     2761 ACACACCAGA GAGCTTGACC CTGACAGTGT GTCAATGTGA TGACCGGAGC ATGTGCAGAG 
     2821 CCCCTATCCC GAGCAGAGAG CCTAACACAT ATGGGGAGTC ATCCTGGAGG CTGGGGCCTG 
     2881 CTGCCATTGG CTTGATCCTG CTAGGACTCC TGATGTTGCT ATTGGCCCCA CTTCTGCTTC 
     2941 TGACCTGTGA CTGTGGGTCA GGTCCCATTG GAGGAGCAGC GACAGGTGGC TTTATCCCAG 
     3001 TGCCCGATGG TTCGGAAGGT ACAATTCATC AGTGGGGAAT CGAAGGAGCT CAGCCCGAAG 
     3061 ACAAGGAAAT CACAAATATT TGTGTTCCTC CAGTTACAAC CAATGGAGCC GATTTTATGG 
     3121 AAAGCTCTGA AGTTTGCACA AATACATATG CTGGTGGGAC CATGGTAGAA GGTGCTTCAG 
     3181 GAATGGAAAT GATTACCAAG CTGGGAGGAG CTACTGGAGC TACAGCGGCA CTTGGTCCCT 
     3241 GTTCCTTGGG TTATTCAGGA ACAATGAGAA CAAGGCATTC CACTGGAGGG ACCTTGAAGG 
     3301 ACTATGCTGC GCCAGTCAAC ATGACTTTCC TAGGCTCCTA TTTCTCTCAG AAATCACTCG 
     3361 CCTATGCCGA GGAAGAGGAT GAGCGCGAAG TGAACGACTG TTTGCTGATC TACGATGATG 
     3421 AAGGCGAGGA TGCTGCCCCC CACTCCCCCA CCCTCAGCTC CTGCAGCATT TTTGCCGATG 
     3481 ACCTAGACGA CAACTTCTTG GATTCCCTTG GCCCCAAATT TAAAAAGCTT GCAGAGATCT 
     3541 GTCTCGGTAT TGATGACGAA GCCAAGCAAG CAAAGCCAGG CCCTAAAGAC AGTGGTTCTG 
     3601 GGGCTGATAC CTGCGCTCGC TCTATGGAAG TCCCTCAGTC AGGTTCTAAC AGGTACCAGA 
     3661 CTTTGCCTGG CAGTCTGGAA GTCACCCAAA CGGGTTCTAA AATATGCCAC ACGCTGTCAG 
     3721 GCAACCAAGA AACTTCTGTT ATGTCCACTT CTGGGTCTGT CCACCCTGCT GTTGCCATCC 
     3781 CTGACCCTCT ACAGCTTGGG AACTACTTGC TCACAGAAAC TTACTCAACT TCAGGTTCCT 
     3841 TTGCACAACC CACCACTGTT ACCTTTGACC CCCATGTCAC ACAAAATGTC ACTGTAACAG 
     3901 AAAGGGTCAT TTGTCCCCTT CCTAGTGCCT CCAGCAGTAT AGTGGCTCCC ACAGAACTGC 
     3961 GAGGCTCATA CAATATGCTC TATACAAAAG AAACCTGTTC CCATCTAACG CGTACGCGGC 
     4021 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4081 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4141 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4201 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4261 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4321 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4381 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4441 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4501 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4561 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4621 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4681 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4741 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4801 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4861 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4921 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4981 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5041 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5101 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5161 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5221 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5281 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5341 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5401 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5461 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5521 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5581 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5641 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5701 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5761 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5821 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5881 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5941 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6001 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6061 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6121 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6181 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6241 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6301 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6361 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6421 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6481 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6541 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6601 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6661 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6721 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6781 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6841 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6901 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6961 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7021 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7081 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7261 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7321 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7381 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7441 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7501 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7561 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7621 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7681 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7741 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7801 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//