LOCUS       MR220390                7404 bp    DNA     circular  SYN 
DEFINITION  Fstl4 (Myc-DDK-tagged) - Mouse follistatin-like 4 (Fstl4)
ACCESSION   NM_177059
VERSION     NM_177059.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3561..3652 
                     /label="Myc-DDK"  
     misc_feature    3682..4267 
                     /label="PolyA signal"  
     misc_feature    3739..3758 
                     /label="Restriction Enzyme"  
     misc_feature    4543..5215 
                     /label="ColE1"  
     misc_feature    5645..6439 
                     /label="Kan/Neo"  
     misc_feature    6474..6776 
                     /label="SV40 ori"  
     misc_feature    6862..7339 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3543..3549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3543 
                     /label="Fstl4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACCAGGA GGCTTCTGGC CACATCTTGC CCTACTCGGG GTCTCCCTGC 
     1081 CGGCCGTGCT GGGATGGATG GACCAAGGAG CCAGCAGAAG CCCGAATATG GTTCCAGGAG 
     1141 AGTCGCAGGC AGAGGAAACC AGAGGCTTTG AAGTCACAAG AAGAGAAGGT CTTCCCAGCC 
     1201 CTGGGCTGTC AGCCTCCTGT GGGAAGAAAC TCTGCAGCCA TGGGAGCAGG TGCTTGCTCA 
     1261 ATAGGACGAC AGGGCAGCCC TCGTGCCAGT GTCTGGAGGT GTGCAGGCCC CGCTACATGC 
     1321 CTGTGTGTGG CTCCGACGGG AGGCTCTACG GGAACCACTG TGAGCTTCGC CGTGCAGCCT 
     1381 GCCTGCTGGG CAAGCGAATT GTCAGTGTAC ACAGCAAGGA CTGCTTTCTC AAAGGAGACA 
     1441 TGTGCACCAT GGCTGGCTAT GCTCGTCTCA AAAATGTCCT CCTGGCACTC CAGAGCCGCA 
     1501 GACAGCCGCT CCCACAAGGA ACCCCCAGGC AAAGCCTCGC CTCCCAGAAG CGCCTCTTGG 
     1561 TGGAGTCACT GTTTAAGGAC TTGGATGCCG ACGGCAATGG GCACCTCGGC AGCTTGGAGC 
     1621 TGGCTCAGTA TGTGCTAAAG GAGCAGGACA TGGACGGTTC ACTGAACAGA TGCTCACCCA 
     1681 GCGACCTCCT GCGATTTGAT GACTATAATA GCGATGGCTC CTTGACCCTT GGAGAGTTCT 
     1741 ACACAGCCTT CCAGGTGATT CAGCTAAGCC TCGCCCCTGA GGACAAGGTC AGTGTGACTA 
     1801 CCGTGACCGT GGGCCTGAGC ACAGTGCTGA CCTGTGCTAT TCGTGGAGAC CTGAGGCCAC 
     1861 CAATCATCTG GAAACGCAAC GGGCTCACTC TGAGCTTCCT AGGCCTGGAA GACATCAATG 
     1921 ACTTTGGCGA GGATGGTTCC CTGTACATCA CCAAGGTGAC CACAGTCCAC ATGGGCAACT 
     1981 ACACCTGCCA CGCCTTGGGC CATGAGCAGC TGGTCCAGAC CCACGTCCTG CAGGTGAATG 
     2041 TGCCACCAGT CATTCGTGTC TATCCAGAAA CCCAGGCACA AGAACCTGGG GTGGCAGCCA 
     2101 GCCTTCGATG CCATGCTGAG GGCATCCCCC TGCCCAGAAT CATTTGGCTG AAAAACGGCA 
     2161 TGGATGTCTC AACCCAGATG TCCAAACAGC TCTCCCTCTT GGCCAATGGA AGCGAACTTC 
     2221 ACATCGGCAG TGTCCGCTAT GAAGACACCG GAGCATATAC CTGCATTGCC AAGAATGAAG 
     2281 TGGGTGTGGA TGAAGATATC TCCTCACTCT TCATTGAGGA CTCTGCTAGG AAGACATTGG 
     2341 CAAACATCTT GTGGCGGGAA GAAGGCCTCA GCGTGGGAAA CATGTTCTAT GTCTTCGCTG 
     2401 AGGATGGCAT CGTTGTCATC CACCCTGTGG ACTGTGAGGT CCAGAGACGC TTGAAACCTA 
     2461 CAGAGAAGAT CTTCATGAGT TATGAAGAAA TCTGTCCTCG TGTGGAAGGA GATGCTACCC 
     2521 AGCCATGCCA GTGGGCCTCT GCAGTCAACG TCAGAAACCG GTACATCTAC GTGGCCCAGC 
     2581 CAGCACTGAA CAGGGTCCTT GTGGTCGACG TTCAAGCCCA GAAGGTCCTA CAGTCCATAG 
     2641 GTGTGGACCC TCTGCCAGTC AAGCTGTCCT ATGACAAGTC ACATGACCAA GTGTGGGTCC 
     2701 TGAGCTGGGG AGACATGCAC AAGTCCCAAC CAAGCCTACA GGTGATCACA GAAGCCAGCA 
     2761 CTGGCCAAGG CCAGCACCTC ATCCGAACAC CGTTTGCAGG AGTGGACAAT TTCTTTATAC 
     2821 CGCCAACAAA TCTCATCATC AACCATATCA GGTTTGGCTT CATCTTCAAC AAGACTGACC 
     2881 CTGCTGTCCA CAAAGTTGAC CTGGAGACTC TGATGGCACT GAAGACAATC AGCCTACGTC 
     2941 ACTACGGCTG CATGCCTCAG GCCATGGCAC ACACCCACCT TGGTGGCTAC TTCTTCGTGC 
     3001 AGTGTCAACA GGACACACCT ACCTCCACCG GCCCACAGCT GCTCATTGAC AGTGTCACAG 
     3061 ACTCGGTGCT AGGCCCCAAC AGTGATATCA CAGGCACGCC CCACGTGTCC CCTGACGGAC 
     3121 GCTTCATCGT CAGTGTCTCC AACAAGGGCC CCTGGCTGCA CGTGCAGGAG GTCACAGTGC 
     3181 GGGGGGAAAT CCAGACGCTA TACGACCTAA AAATAAACCC AGGCATCTCC GACTTGGCAT 
     3241 TCCAGCATTC CTTCACAGAA GGTAGTCAGT ACAACGCCTA TGCCACTCTC GACAAGGAGC 
     3301 CAGACCTGCT CTTCCTGGAG CTGTCCACTG GGAAGATGGG CAGGCTGAAG AACTTAAAGG 
     3361 AGCCACCCAG AGGGCCAGCC CCAACCTGGG GTGGACCCCG CAGAGTCCTG AGAGACAGCG 
     3421 GGCTCTTTGG ACAGTACCTC CTCACACCAG CTCAAGAGTC ACTGTTCCTG GTCAATGGGA 
     3481 GACAAAACGC CCTGAGGTGT GAGGTTTCAG GCATAAAGGG TGCGGCCACA GTGGTGTGGG 
     3541 TCGGGGAGGT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3601 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3661 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3721 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3781 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3841 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3901 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3961 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4021 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4081 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4141 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4201 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4261 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4321 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4381 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4441 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4501 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4561 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4621 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4681 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4741 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4801 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4861 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4921 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4981 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5041 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5101 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5161 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5221 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5281 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5341 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5401 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5461 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5521 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5581 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5641 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5701 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5761 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5821 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5881 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5941 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6001 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6061 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6121 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6181 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6241 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6301 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6361 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6421 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6481 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6541 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6601 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6661 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6721 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6781 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6841 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6901 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6961 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7021 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7081 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7141 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7201 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7261 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7321 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7381 TAACAAAAAT TTAACGCGAA TTTT
//