LOCUS       MR220313                8304 bp    DNA     circular  SYN 
DEFINITION  Col28a1 (Myc-DDK-tagged) - Mouse collagen, type XXVIII, alpha 1 (Col28a1)
ACCESSION   NM_001037865
VERSION     NM_001037865.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4461..4552 
                     /label="Myc-DDK"  
     misc_feature    4582..5167 
                     /label="PolyA signal"  
     misc_feature    4639..4658 
                     /label="Restriction Enzyme"  
     misc_feature    5443..6115 
                     /label="ColE1"  
     misc_feature    6545..7339 
                     /label="Kan/Neo"  
     misc_feature    7374..7676 
                     /label="SV40 ori"  
     misc_feature    7762..8239 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4449 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="Col28a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAGAAGA GACGTTGCCT TCTGTCTTCT GCTTTTGCCA GCTTTTATGA 
     1081 CTCAAGCAGT GTATGGACAA AGAAAGAAAG GACCAAAGCC AAACACACTT GCAAGGAAAA 
     1141 ATGACTTCCA GGATGCCATT TGCTTCATAG ATGTTGTCTT TATCTTGGAC AGCTCAGAAA 
     1201 GCTCCAAAAT TGTACTCTTT GATAATCAGA AAGATTTTGT GGACAGCTTG AGTGAGAAGA 
     1261 TTTTCCAGTT GACTCCTGGC CGTTCATTAA AATATGACAT CAAGCTGGCA GCCCTTCAGT 
     1321 TTAGCAGCTC GGTCCAGATT GATCCACCCT TGTCTTCTTG GAAGGATCTC CGGACATTTA 
     1381 AGCAGCGGGT CAAGTCTCTG AATTTAATTG GGCAAGGTAC CTTTTCTTAT TATGCCATCT 
     1441 CCAATGCCAC AAGGCTACTT AAAAGAGAAG GGCGTAAAGA TGGCGTGAAG GTGGCTTTGC 
     1501 TGATGACTGA TGGCATCGAT CATCCCAAGA GCCCAGATGT CCAAAGTATT TCTGAAGATG 
     1561 CTAGAATTTT GGGAATATCA TTTATCACCG TTGGACTTTC CACTGTGGTC AATGAAGCCA 
     1621 AACTTCGTTT GATATCTGGG GATCCTTCCA ATGAACCCGT TCTACTATTG AGTGACCCAA 
     1681 CACTTGTAGA CAGAATTCAG GAACGCCTGG GTGTCTTGTT TGAAAGGAAG TGTGAACACA 
     1741 AGATCTGTGA ATGTGAAAAG GGCGAGCCAG GTGACCCAGG GCCTCCTGGT ACCCATGGAA 
     1801 ACCCTGGTAT CAAAGGTGAG CGAGGACCAA AAGGAAACCC GGGTGATGCT CAGAAAGGAG 
     1861 AAACTGGAGA AAGAGGTCCT GTGGGAATCC CTGGGTACAA GGGTGACAAG GGTGAACGAG 
     1921 GAGAATGTGG GAAACCAGGA ATGAAAGGTG ACAAAGGACC TGAAGGGCCA TATGGACCAA 
     1981 AGGGACCAAG AGGAATTCAG GGCATTGGTG GACCTCCAGG GGATCCAGGT CCAAAGGGAT 
     2041 TTCAAGGAAA TAAGGGTGAG CCAGGTCCTC CTGGTCCTTA TGGTCCTCCA GGAGCCCCTG 
     2101 GTATTGGACA GCAAGGTGTT AAGGGTGAGA GGGGCCAGGA AGGAAGAATG GGAGCTCCCG 
     2161 GACCGATTGG AATTGGTGAA CCCGGACAGC CAGGTCCCCG TGGTCCTGAG GGAGCACCAG 
     2221 GAGAGCGGGG CCTACCAGGA GAAGGATTTC CTGGACCTAA GGGTGAAAAG GGATCTGAAG 
     2281 GACCAATCGG CCCACAAGGA TTACAAGGCC TGTCAATCAA AGGGGATAAG GGGGATTTGG 
     2341 GACCTGTGGG ACCGCAGGGG CCAGCGGGAA TTCCTGGCAT CGGAAGCCAA GGCGAGCAGG 
     2401 GGATCCAAGG TCCATCTGGT CCACCTGGTC CTCAAGGACC CCCAGGACAA GGCTCACCTG 
     2461 GTCCTAAGGG AGAAGTAGGT CAGATGGGAC CCACAGGGCC TAGAGGACCA ATGGGCATTG 
     2521 GAGTACAGGG TCCAAAGGGG GAGCCAGGCA CAGTGGGGCT CCCAGGACAG CCTGGAGTCC 
     2581 CAGGGGAAGA TGGGGCCTCT GGAAAGAAGG GAGAAGCTGG ACTTCCAGGA ACAAGAGGCC 
     2641 CAGAAGGAAT GCCTGGGAAA GGACAGCCCG GCCCCAAGGG TGATGAAGGA AAGAAAGGGA 
     2701 GCAAAGGAAA TCAAGGACAG AGGGGGTTTC CAGGACCCGA AGGGCCAAAG GGAGAGCCAG 
     2761 GTGTTATGGG CCCCTTCGGA ATGCCCGGAG CCTCAATTCC TGGACCATCT GGACCAAAGG 
     2821 GAGACCGAGG AGGACCTGGC ATGCCTGGTC TTAAAGGAGA ACCTGGACTT CCTGTTCGAG 
     2881 GACCAAAGGG TGCCCAGGGT CCCCGAGGAC CAGTGGGGGC TCCAGGACTC AAAGGTGATG 
     2941 GCTATCCTGG TGTGGCTGGA CCGCGAGGAT TGCCAGGACC CCCTGGACCA ATGGGCTTAC 
     3001 GTGGTGTGGG AGACACTGGA GCAAAGGGAG AACCAGGAGT CAGAGGCCCT CCAGGTCCCT 
     3061 CTGGGCCTCG GGGAATAGGA ACCCAAGGAC CAAAGGGTGA TACTGGGCAA AAGGGCCTGC 
     3121 CTGGCCCTCC TGGGCCCCCT GGCTATGGAT CACAAGGAAT TAAAGGGGAG CAAGGACCTC 
     3181 AAGGTTTCCC AGGGTCAAAG GGCACAGTGG GCCTTGGCCT CCCTGGTCAG AAGGGAGAGC 
     3241 ATGGAGACCG GGGTGATGTG GGAAGGAAAG GCGAAAAGGG AGAAACTGGA GAGCCTGGGT 
     3301 CTCCAGGAAA ACAGGGGTTA CAAGGACCTA AAGGAGACCT GGGACTCACA AAAGAAGAAA 
     3361 TTATCAAACT TATCATTGAA ATATGTGGTT GTGGGCCCAA ATGCAAAGAG ACTCCACTGG 
     3421 AGTTGGTGTT TGTGATTGAC AGTTCAGAAA GTGTGGGACC AGAGAACTTT CAGATCATCC 
     3481 AAAGTTTTGT GAAGACTTTG GCTGACCGGG TGGCCTTGGA CCTTGGCACA GCCCGGATAG 
     3541 GCATAATCAA CTATAGCCAC AAGGTGGAGA AGGTGGCTAG CCTGAAGCAG TTTTCCAGCA 
     3601 AGGATGACTT CAAGCTGGTG GTAGACAACA TGCAGTATCT GGGGGAAGGC ACCTATACAG 
     3661 CCACTGCTCT TCAAGCAGCT AATGACATGT TCAAAGAGGC AAGGCCAGGT GTAAAGAAGG 
     3721 TGGCCTTGGT GATCACGGAT GGACAGACAG ATTCCCGAGA TAAAAAGAAG CTGGCAGATG 
     3781 TGGTGAAGGA TGCCAATGAC TCCAACGTGG AAATATTTGT CATTGGAGTG GTGAAGAAGG 
     3841 ATGACCCCAA CTTTGAAATT TTTCACAAAG AAATGAATCT CATTGCTACT GATGCAGAAC 
     3901 ATGTGTATCA GTTTGACGAC TTCTTTACCC TGCAAGATAC TCTGAAGCAA AAGTTGTCTA 
     3961 AAAAAATTTG TGAAGATTTT GACTCCTATC TCATTCAAGT TTTTGGATCA CCATCTTTTC 
     4021 AACCTGAATT TGGGGTATCA GAACGAGAGG TCAGCGTATC CACTCCAAAG CCTGCAAAAG 
     4081 AAATGTCTAA GTCGTTTAAT GTTTCTAGAG GCCAAAATGA AGAGACTGAA TCGTATGTGT 
     4141 TGACTGAGGC TGGTATCTTG GCCATCCCAA CCCCACCTGA AGCCACCAAT ACACTTGAGC 
     4201 CATTACTCAG CAGCCGTGAA GGAGTGGAAA CAAGAACTCC AAATCCCAAT TTGTTACAGT 
     4261 CAGAAAAATC TCTGTACAAA GATCCAAGAT GCGAGGAAGC CCTGAAGCCC GGGGAATGTG 
     4321 GCGACTACGT GGTTCGCTGG TATTACGACA AACAGGTCAA CTCTTGCGCT CGCTTTTGGT 
     4381 TCAGTGGCTG CAATGGCTCA GGAAACAGAT TCCACAGTGA AAAAGAATGT CGAGAAACCT 
     4441 GCATTAAACA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4501 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5281 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5341 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5401 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5461 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5521 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5581 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5641 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5701 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5761 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5821 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5881 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5941 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6001 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6061 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6121 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6181 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6241 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6301 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6361 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6421 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6481 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6541 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6601 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6661 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6721 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6781 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6841 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6901 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6961 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7021 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7081 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7141 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7201 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7261 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7321 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7381 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7441 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7501 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7741 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7861 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7921 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7981 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8041 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8101 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8161 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8221 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8281 TAACAAAAAT TTAACGCGAA TTTT
//