LOCUS       MR220169                7602 bp    DNA     circular  SYN 
DEFINITION  Itih1 (Myc-DDK-tagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (Itih1)
ACCESSION   NM_008406
VERSION     NM_008406.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3759..3850 
                     /label="Myc-DDK"  
     misc_feature    3880..4465 
                     /label="PolyA signal"  
     misc_feature    3937..3956 
                     /label="Restriction Enzyme"  
     misc_feature    4741..5413 
                     /label="ColE1"  
     misc_feature    5843..6637 
                     /label="Kan/Neo"  
     misc_feature    6672..6974 
                     /label="SV40 ori"  
     misc_feature    7060..7537 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3741..3747 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3741 
                     /label="Itih1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGGTGCC ACAGGGCTCC GGGTGCTGCT GTGCTTGCTG CCGCTCCTCA 
     1081 CTCTGCAGGC CAGGCCTGCT TTGGGCTTGG CAACAGGCAG ACCCAGGGGC AGTGAGAAGC 
     1141 GACACGCTGT GGACACTTCA GTCAATGGTG TGTCCATCAA GAGCTTGAAA GTCAATTGCA 
     1201 AAGTCACCTC TCGCTTTGCC CACTACGTCA TCACCAGCCA AGTGGTCAAC AATGCTGATA 
     1261 AAGCCAGGGA AGTGGCTTTT GATGTGGAAA TCCCCAAGAC AGCCTTCATC AGTGACTTTG 
     1321 CCATAACATC AGATGGGAAG GCGTTCATTG GGGACATAAA GGACAAAGTG ACTGCATGGA 
     1381 AGCAGTACCG GAAGGCAGCC GTTTTAGGGG AGAGCGCTGG CCTTGTCAGA GCCTCGGGCA 
     1441 GAAATATGGA GCAGTTCACC ATCCATATCA CTGTTGGAGC CCAGAGCAAG GCCACGTTCC 
     1501 GGCTCACCTA TGAGGAGGTA CTAAAGAGGA GACTGATGCA GTATGACATC ACCATCAAAG 
     1561 TCAGACCCAA GCAGCTGGTA CAGCATTTTG AGATCGATGT GGACATCTTT GAACCCCAGG 
     1621 GGATCAGCAA GCTGGACGCT CAGGCCTCCT TCCTCAGTGA GGAACTGGCT GCTCAAACCA 
     1681 TCAAGAAGTC TTTCTCAGGG AAAAAGGGTC ATGTGCTCTT CCGCCCCACT GTGAGCCAGC 
     1741 AGCAGTCCTG CCCCACATGT TCTACATCCT TGCTAAATGG AGAATTCAAG GTGACCTACG 
     1801 ATGTCAATCG AGACAAGCTC TGTGACCTCC TGGTGGCCAA CAACTACTTT ACACATTTCT 
     1861 TTGCCCCCAA AAACCTGACC AACATGAGCA AGAACCTGGT TTTTGTGATT GACATCAGTG 
     1921 GCTCCATGGA AGGCCAGAAA GTGAGGCAGA CAAAGGAGGC ACTCCTTAAG ATCCTGGAGG 
     1981 ACATGAGGCC AGTAGACAAC TTTGACCTGG TCCTCTTTGG CTCTAAAGTG CAATCATGGA 
     2041 AGGGCTCCCT GGTACCTGCG TCTAATGCCA ATCTGCAAGC AGCTCAAGAC TTTGTGAGGC 
     2101 GCTTTTCTTT GGCTGGAGCC ACAAACCTGA ATGGAGGCTT GCTCCGAGGA ATCGAGATCT 
     2161 TAAACAAAGC TCAAGGAAGC CACCCAGAAC TCAGCAGCCC GGCCTCAATT CTTATCATGT 
     2221 TGACAGACGG AGAGCCCACT GAAGGGGAGA CGGACCGTTC CCAGATCCTC AAGAATGTAC 
     2281 GCAACGCTAT CCGGGGCAGG TTCCCGCTCT ACAACCTCGG CTTTGGCCAC GACCTGGACT 
     2341 TCAGCTTCCT GGAGGTCATG TCCACTGAGA ACAACGGATG GGCCCAGAGA ATTTATGAGG 
     2401 ACCATGATGC CACCCAACAG CTACAGGGTT TCTACAATCA AGTAGCCAAT CCCCTGCTGA 
     2461 CAGATGTGGA ACTGCAGTAC CCCCAGGATG CTGTCTTGGC TCTAACCCAG CACCGACATA 
     2521 AACAGTACTA TGACGGCTCG GAGATTGTGG TGGCTGGGCG CATTGCTAAC CACAAACTGA 
     2581 ACACCTTTAA GGCTGACGTT CGGGCTCGTG GGGAAAAGCA AGAATTCAGG GCAACCTGCC 
     2641 TAGTGGACGA GGAAGAGATG AAGAAACTGC TCCGAGAGCG TGGCCACGTG CTAGAGAATC 
     2701 ATGTGGAGAG GCTCTGGGCC TACCTCACGA TCCAGGAGCT CCTGGCTAAG CGGATGAAGA 
     2761 CGGAAGGGGA GGAGAGGGCC AACCTGTCAT CCCAGGTCCT GAAGATGTCA CTGGACTATC 
     2821 ATTTCGTAAC GCCACTGACC TCTCTGACTA TCAGAGGCCT GACAGACGAG GATGGGCTGG 
     2881 AGCCCACCAT TGACAAGCCC CTGGAGGATT CTCAGCCCTT AGAGATGGTG GGACCCAGAA 
     2941 GAACGTTTGT GCTGTCAGCC ATACAGCCAT CTCCTACAGC CCACCCCATA GACTCAAAGT 
     3001 TGCCACTCCG TGTGACAGGT GTGGACACCG ATCCCCACTT CATCATCTAT GTGCCCTCAA 
     3061 AAGAGGATAG CTTGTGCTTC AACATCAATG AGGAACCTGG TGTGATCCTG AACCTAGTAC 
     3121 AGGACCCTGA CACAGGCTTC ACCGTGAATG GGCAGCTCAT TGGGAACAAG GCCAGCAGCC 
     3181 CTGGGCAACA CGAGAGCACA TACTTCGGGA GATTGGGAAT CTCAAGTCCC ACATCAGACT 
     3241 TTCAGCTGGA AGTGACTCCT CAGAACATTA CACTGAACCC CAGCTCCAGC GGGTCCATGT 
     3301 TCTCCTGGAG GGACCAGGCT GTGCTGCAGA AGGATGGGGT AGTGGTGACA ATCAACAAGA 
     3361 AGAGGAACCT GGTGGTGTCT GTGGATGATG GGGCTACCTT TGAGATTGTC CTGCACAGAA 
     3421 CATGGAAGGG GAGTGCAGTC CACCAGGACT TCCTGGGTTT CTATGTGCTG GACAGCTTCC 
     3481 GGATGTCAGC CCGGACAAAG GGGCTACTGG GACAATTCTT CTCCCCACTG GATTTTGAAG 
     3541 TATTTGACCT CCACCCAGGC TCTGACCCTA CAAAGACAGA TGCCACAATG GTGGTGAAGA 
     3601 ATCGGCAGCT GACAGTCACC CGAGGGTTGC AAAAAGACTA CAGCAAGGAC CCCAGACATG 
     3661 GAGCAGAAGT GCCTTGCTGG TTTGTCCACG ACAATGGAGC TGGACTGATT GATGGTGTTC 
     3721 ACACTGACTA TGTCGTCTCT GACATCTTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3781 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3841 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3901 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3961 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4021 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4081 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4141 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4201 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4261 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4321 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4381 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4441 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4501 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4561 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4621 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4681 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4741 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4801 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4861 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4921 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4981 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5041 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5101 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5161 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5221 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5281 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5341 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5401 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5461 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5521 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5581 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5641 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5701 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5761 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5821 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5881 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5941 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6001 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6061 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6121 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6181 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6241 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6301 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6361 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6421 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6481 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6541 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6601 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6661 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6721 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6781 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6841 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6901 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6961 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7021 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7081 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7141 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7201 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7261 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7321 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7381 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7441 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7501 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7561 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//