LOCUS       MR220009                7134 bp    DNA     circular  SYN 
DEFINITION  Adam5 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 5 (Adam5)
ACCESSION   NM_007401
VERSION     NM_007401.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3291..3382 
                     /label="Myc-DDK"  
     misc_feature    3412..3997 
                     /label="PolyA signal"  
     misc_feature    3469..3488 
                     /label="Restriction Enzyme"  
     misc_feature    4273..4945 
                     /label="ColE1"  
     misc_feature    5375..6169 
                     /label="Kan/Neo"  
     misc_feature    6204..6506 
                     /label="SV40 ori"  
     misc_feature    6592..7069 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3273..3279 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3273 
                     /label="Adam5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCTCCTT CTTTTGCTCT TTTTGCATCT TAAAGGGCTG CAGGCAGGCC 
     1081 AGAATCCTCA GAAAACCACC CTGCAGACAA CTGTTCCTGA AAAGATCTCA TCACCGGATG 
     1141 TGGAAACAGA TGCAGAAGAC CATATGGCTT ATCTTATTAC AATAAATGAA ACCCCCCATT 
     1201 TCATCCATCT TAAAAAACAA TCATTTATAA CTCCAACTGC AGTTGTTTAT ACTTACGACA 
     1261 GAAATGATGT TCAACATTCT CAACCTTTGT CAGCTCTGGA AAATTGCAAT TACAATGGAT 
     1321 ATGTTGCAGG TTTCCCAAAC TCTATCGTGA CCCTCACCGT TTGCACAGGA CTCAGGGGAA 
     1381 TCATACAGTT TGAAAATGTC TCCTATGCAA TCGAGCCCGT GGAAACCCTG TCAGGATTTG 
     1441 TGCACGTGAT CTATGAGAAC ACAAACAAAC ATGCTGTGAT TCCTGACCTG GGAAAGAATC 
     1501 AGTCATACAG TTGGTTTGAC GAATCAGATT ATCAATTCCG GAGTAACATG AAGAAATCTG 
     1561 GATTTACCGT GTTACGCCAA CGATTCATTA TGATGGATAT CATCGTGGAT AAAAAGCTGT 
     1621 TTGATTACAT GGGTTCTGAC ACTGAAGTTG TGCTACAGAA AGTCATTCAG ATCATCGGTT 
     1681 TCGTTAACAC TATGCTCTCC AAGTTAAAAC TGACTGTCTT AATAAATTCC ATTGAAATCT 
     1741 GGTCGAAGGA AAACAGAATT CGTTTATCAA AGGCAGTTGA TGACTTATTG GTTCAGTTCT 
     1801 CAATTTGGAA ACATGAGTAT AGATCACAAC ATGTTGCATA CTTACTGGCA TTTGAGGAGC 
     1861 ACCCTGCTTC CACGGGGGCT CTATATCCAG GGAATCTGTG CAAACTGGAG TACAATGCTG 
     1921 CTGTTGCCCT GTATCCAAAA GGTTTATCGT TAGAATCGTT TAGTGTCATT GTCCTGCAGC 
     1981 TGCTGAGCAT TGGTATGGGC TTAACTTACG ACACTGAGAA CTGCCACTGT ACAGGAGAAG 
     2041 TTTGCCTAAT GACGCCCAAA GCAATATATT CTGGGGGTGT GAAGGACTTC AGTACTTGTA 
     2101 CCCTGGATGA CTTTAAATAC CTGTCAACAA GACAAGACCT TAGATGTCTC CAGGACTTGC 
     2161 CTTTGGAAAG AAAGCCAGCT CGCAGGCCAA GGAGAATCTG TGGCAATGGG ATACTGGAGA 
     2221 TGAACGAGCA ATGTGACTGT GGCACTTTGA AGAACTGTAC ACATAGAAAA TGCTGTGACC 
     2281 CTATGTCATG TCGATTGAAA AACAAGGCCA CGTGTGGTTC TGGGGAGTGC TGTAGTCAAG 
     2341 ATTGCACGGT AAAAATGAAT GATGTGGTCT GTAGAAAGTC GGTAGATGAA TGTGACTTTG 
     2401 TGGAGTACTG TAACGGAAAA GATCCCTATT GTGTCCCTAA CACATATGCA CGCAATGGGC 
     2461 AGTATTGTGA ATCAGGTGAA GCCTTCTGTT TTGAAGGACG ATGTCAGACT GCTGACAAGC 
     2521 AATGTATGAG CATGCTTGGG AAATACGTGA GAGGGGCTTC CTTTGCGTGC TATGAAGAGT 
     2581 TCAATTCCAG AGGCGATAGA TTTGGCAACT GTATTCATAA TTTTTGTGCT TTCAGAAATT 
     2641 CTCTCTGTGG GAAACTAATC TGTACGTGGC CATTCAAGAA GCTGGTGCTA AAAGCCAATT 
     2701 TATCTGTGGC CTATGCGCAA ATCCGGGATG ACTTGTGTGT AGCTATGTAT AAAGGTGGGA 
     2761 GGATACCTAA GACAACAAAG ACGACATATT CCAATCCAGC GGACAGAGAT GAGACATTTG 
     2821 TAAACGACGG CACCATCTGT GGCCCTGATA TGTTTTGTTT GAGAGCATCA TGCACAGAAA 
     2881 CTAGATTTCA CATGGATTCT TCCAAGTGTG ATTCAACTAG AGATTGCAAC GACCATGGGG 
     2941 TTTGCAACAA TTTACAGCAT TGCCACTGTG ATATAGGATA TAACCCTCCT TTTTGTGAGG 
     3001 AACATAAGGG ACAGTTTGGA AGCGTTGATG ATGGACATAA ATATCATGTT GAAGATGGTA 
     3061 AAAGCTATAA GCAGCAAAGT CATAGTAATC TTAAAAAAAA TCAGCTTCAG CTCATTCTCT 
     3121 ACATATCTCT GCCCTTACTT GTGATGATTT CAGCTGTAGT TATAAAGCAA AGCAAACTGA 
     3181 GTCGTGTATG TGACAGAGAA AGAAGTGAAA GCGACAGCTC TACAACAGAA GATAGCGGGA 
     3241 GTAACACCAA TGTGACCAGC AGTGGAGGAA GCACCTCCCA CACGCGTACG CGGCCGCTCG 
     3301 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3361 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3421 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3481 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3541 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3601 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3661 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3721 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3781 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3841 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3901 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3961 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4021 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4081 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4141 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4201 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4261 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4321 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4381 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4441 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4501 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4561 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4621 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4681 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4741 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4801 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4861 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4921 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4981 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5041 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5101 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5161 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5221 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5281 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5341 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5401 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5461 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5521 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5581 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5641 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5701 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5761 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5821 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5881 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5941 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6001 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6061 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6121 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6181 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6241 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6301 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6361 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6421 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6481 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6541 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6601 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6661 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6721 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6781 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6841 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6901 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6961 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7021 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7081 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//