LOCUS       MR219954                7080 bp    DNA     circular  SYN 
DEFINITION  Lrrn4 (Myc-DDK-tagged) - Mouse leucine rich repeat neuronal 4 (Lrrn4)
ACCESSION   NM_177303
VERSION     NM_177303.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3237..3328 
                     /label="Myc-DDK"  
     misc_feature    3358..3943 
                     /label="PolyA signal"  
     misc_feature    3415..3434 
                     /label="Restriction Enzyme"  
     misc_feature    4219..4891 
                     /label="ColE1"  
     misc_feature    5321..6115 
                     /label="Kan/Neo"  
     misc_feature    6150..6452 
                     /label="SV40 ori"  
     misc_feature    6538..7015 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3219..3225 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3219 
                     /label="Lrrn4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTGGACG CTGATGCTGC AGCTGCTGCA GCTCCTGCTA CAACTGCTGA 
     1081 TGGCGCAGAG CCAGAGCCTG GAAAGGATCT CCCAGGACCG GATCCCCCTC TTCCGACTCA 
     1141 CTCAGCAGGG AGACTGGGAC AGCCTCGACC GCCACCCTAC CGACTCTCTC TGTGTAGGAC 
     1201 TCCCCGCCGC GGGAGTCACC ACTCTGAACT TGGCGAACCG CAGCCTGGAG AGCCTGCCTA 
     1261 GCTGCCTGCC GCGCACGCTG CGCAGCCTCG ATGGCAGCCA CAACCTGCTG CGCGCCCTTA 
     1321 GCGAGCCTGT GCTCGGCCGC CTGCCCGAGC TGCGCGTACT CACGCTGCAC CACAACCGCA 
     1381 TCTCCGTGCT ACACTGGGGC CGTGACACGC TGGCCGAGCT GCGTGAGCTC GACCTCAGTC 
     1441 ACAACCTGCT GACCGAGCTG CCGCCCTGCG CGGGGCCCTC GGGGAGCAGC CTGCGCTCGC 
     1501 TGGCGCTGGC CGGGAACCCA CTGCGAGCGC TGCTGCCACG GACCTTTGCA TGCTTCCCAG 
     1561 CGCTGCGGCT TCTCAACCTC TCCTGCAGCG AGCTGGGCCA CATCGCCCAG GAGGCATTCG 
     1621 CGGGGGTGGA CGGTGGGCCC CTGGCGGCAC TGGAGCTCTT GGATCTGAGC GGCACGAGCC 
     1681 TAGAAAGAGT TGAGTCTGGG TGGATCAGAA ACCTGCCAAA GCTCAAGTCC CTCTTCCTGA 
     1741 GAAAGATGCC CAGGCTGAAG ACACTGGAGG GAGACATTTT CAAGATGACT CCCAACCTGC 
     1801 GGCAGCTGGA TTGTGGAGAC TCTCCAGCAC TTACTTCTGT TCACACAGAG ATCTTTCAAG 
     1861 ACACACCCAA TCTACAAGTC CTTCAGTTTC AGAACTGCAA CTTGAGTTCC TTTGGTCCTT 
     1921 GGAATTCCTC CCAGGTCTTG TCCGTCAGTC TCTTTGGCAA CCCTCTCATT TGCAGCTGTG 
     1981 AGCTGGCTTG GCTTCTCGTG GATGTCAACA AAACTGTCTT ACACAGGGCA GCTGATACCA 
     2041 TGTGTGAGCC TGCTTTAGGA TCCACGGGCC CCTTCTCAGG CCCTCTCTCC CTCTCCCACC 
     2101 TGTCTAATGT GTGCAGGTCA GACCAAAGCA CCACCCTCCT GCCTTCAAAC CCAGGCCGCT 
     2161 TCGATCATTC AGTTTTTGCA CCACGGATAC AGGGGCCTTC CATTGAACAG AGCACGGCCT 
     2221 TGTCTGCTCA GCCTGGAGGA AGCCAACAAA ATATCACCAA GGTCCCTTCC CTCACCATGA 
     2281 CTTCCCCCAC ACAAGGATCA TGGATGTATA AAGATGCTTC AGAGGAAACT GCCCAGTCCA 
     2341 CTAACTCTGA GCTAGTTTAT AGTCCTTCCA GGGCTCTGCC TGGTGCTGCC AGTTCTGGGG 
     2401 CAGAGCAGAC TGCCACACAT ATCCTTGAGC CTAATATTTC ATCTGCCTCT ACCCCATTAG 
     2461 TCAGCAAGTA CCTTGAACCC TTGCCTACTT CACCAAACCC CAGGAGCTTA CCTCAGACCA 
     2521 AGCAGAGGAC ACAAGCCACG CCCAGGGCTC TCCACACAGA CCCTCCACAG GATGAGATTC 
     2581 CAGTTTTGCT GTTAGATGAT GACAGCGAGG AGGAGGAGAC TCGGGACCAG GTGGCGGCAC 
     2641 CTCCCCAGGA CGTCTCTTGT GAATACCACC CCTGCAAGCA TCTGCAGACC CCGTGCGCAG 
     2701 AGCTGCAGAG GCGCTTCAGA TGCCGTTGCC CTGGTCTCAG TGGGGAGGAT ACCACTCCAG 
     2761 ACCCTCCCAC GCTGCAGGGG GTGTCAGAGG TGACTGACAC ATCAGTGCTC GTCCACTGGT 
     2821 GTGCCCCCAA CTCAGTGGTG CTCTGGTATC AGATCCACTA TGTGGCAGAA GGACGATCTG 
     2881 GGAATCAATC CGTAGTGGAC ATCTATGCTA CTGCCAGGCA GCACCCCCTG TACAAGCTGA 
     2941 CACCTGGCAC CACCTACCAT GTGTGTGTAC TGGCAGCCAA CAGGGCTGGC CTGAGCCAGT 
     3001 CACAGACCTC AGGCTGGAGG AGGTCATGTG CCACTTTCAC AACCAAGCCC AGCTCTGTGG 
     3061 TCATCTTCTG GGGACTGTGT ACCGCCAGTG GCCTGTTACT GGTTAGCACC CTGGTGCTGT 
     3121 CTGTGTGTCT CTGGAGGCAG CGCTGGAAGC CACACAGGCA GTTCTACGAC ACACACCTGG 
     3181 TGGCCTTCAA AAACCCAGCC AGAGCTGAGG AAGTAACTCA GTGGGAGACG CGTACGCGGC 
     3241 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3301 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3361 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3421 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3481 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3541 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3601 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3661 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3721 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3781 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3841 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3901 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3961 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4021 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4081 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4141 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4201 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4261 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4321 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4381 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4441 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4501 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4561 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4621 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4681 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4741 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4801 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4861 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4921 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4981 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5041 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5101 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5161 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5221 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5281 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5341 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5401 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5461 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5521 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5581 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5641 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5701 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5761 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5821 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5881 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5941 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6001 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6061 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6121 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6181 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6241 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6301 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6361 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6421 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6481 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6541 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6601 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6661 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6721 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6781 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6841 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6901 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6961 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7021 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//