LOCUS       MR219751                7887 bp    DNA     circular  SYN 
DEFINITION  Ano2 (Myc-DDK-tagged) - Mouse anoctamin 2 (Ano2)
ACCESSION   NM_153589
VERSION     NM_153589.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4044..4135 
                     /label="Myc-DDK"  
     misc_feature    4165..4750 
                     /label="PolyA signal"  
     misc_feature    4222..4241 
                     /label="Restriction Enzyme"  
     misc_feature    5026..5698 
                     /label="ColE1"  
     misc_feature    6128..6922 
                     /label="Kan/Neo"  
     misc_feature    6957..7259 
                     /label="SV40 ori"  
     misc_feature    7345..7822 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4026..4032 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4026 
                     /label="Ano2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCCCT GGGCTGCGAG ACATCCCTCT GCTCCCTGGA TCTCCACGTC 
     1081 GGCTGAGTTC TCGGACCGTG GCCAGAGGAA GCCAGGGCCC CAAACATGGG CAGCAGTACC 
     1141 TCAAAGTGCC AGGTCACCGG GCCCCAGGTC AGAGGGACAA TTCCAGCCTA CACCCTAGCC 
     1201 AGGTCTCCAG GAGAGAGAGT TCCCGAGACC GTTCTGTCAT CAATAACTAC CTGGATGCCA 
     1261 ATGAGCCCCC GTCCTCGGAA GCCCGTCTGA GCCGCATGCA CTTTCACGAC AACCAGAGGA 
     1321 AAGTCGACTA TGTACTTGCA TACCACTACC GGAAACGAGG CGCACACCTG GGTCACGGCT 
     1381 CCCCTGGACA CTCTCTGGCT GTTATCTCCA ATGGGGAGAC AGGCAAGGAG CGCCATGGCG 
     1441 GGGGCCCTGG TGATGTTGAA CTTGGGCCAC TGGATGCTCT GGAAGAGGAA AGGAGGGAGC 
     1501 AGCGAGATGA ATTTGAGCAC AACCTGATGG CTGCTGGGCT GGAGCTGGAG AAGGACTTGG 
     1561 AGAGCAAAAG CCAGGGATCT GTCTTTGTCC GGATCCACGC CCCATGGCAG GTGCTGGCCA 
     1621 GGGAAGCAGA GTTCTTGAAG ATCAAAGTCC CCACCAAGAA AATGTACGAG ATCAAAGCAG 
     1681 GAGGCAGCAT CGCAAAGAAG TTCAGCGCCA TTCTGCAGAC GCTCAGTTCA CCCCTGCAGC 
     1741 CCAGAGTTCC TGAACACAGC AACAACAGGA TGAAAAACCT CTCCTACCCA TTCTCCAGGG 
     1801 AAAAAATGTA CCTGTACAAC ATCCAGGAAA AGGACACCTT CTTTGACAAT GCTACCCGGA 
     1861 GCCGCATTGT ACACGAGATT CTGAAGCGGA CGGCCTGTTC TCGAGCCAAC AACACGATGG 
     1921 GTATTAACTC TCTAATAGCA AACAATATCT ACGAGGCTGC CTACCCGCTT CATGATGGTG 
     1981 AATATGACAG TCCAGGGGAT GACATGAATG ACAGAAAGCT GCTGTATCAG GAATGGGCAC 
     2041 GCTATGGAGT GTTTTACAAA TTTCAACCCA TTGACCTCAT CAGGAAGTAT TTTGGAGAGA 
     2101 AAATTGGGCT ATATTTTGCT TGGCTGGGGT TGTATACATC ATTCCTGATC CCATCTTCTG 
     2161 TCATAGGCGT GATTGTGTTT CTTTATGGAT GTGCAACAAT CGAGGAAGAT ATTCCCAGCA 
     2221 AAGAGATGTG TGACCACCAG AATGCCTTCA CCATGTGTCC CCTGTGTGAC AAGTCCTGTG 
     2281 ACTATTGGAA CCTCAGCTCA GCCTGTGGGA CTGCACGGGC CAGTCATCTG TTTGACAATC 
     2341 CAGCCACCGT CTTCTTTTCC ATCTTCATGG CTCTGTGGGC TACCATGTTT CTGGAAAACT 
     2401 GGAAGAGACT CCAGATGCGG TTGGGCTACT TCTGGGACCT GACTGGGATC GAAGAGGAAG 
     2461 AAGAACGTTC CCAGGAACAC TCTCGGCCTG AATATGAAAC CAAAGTACGA GAGAAACTGT 
     2521 TAAAGGAGAG TGGCAAGTCA GCGGTTCAGA AGCTGGAAGC AAATTCACCT GAGGATGATG 
     2581 AGGATGATGA AGATAAACTG ACTTGGAAAG ATCGTTTCCC AGGATACTTG ATGAACTTTG 
     2641 CCTCCATCTT GTTTATGATT GCCCTGACGT TCTCCATTGT CTTTGGGGTC ATTGTGTATC 
     2701 GGATCACAAC TGCAGCTGCC CTGTCTCTCA ACAAGGCCAC ACGCTCCAAT GTCCGGGTGA 
     2761 CAGTGACTGC AACAGCAGTC ATCATCAACC TCGTGGTCAT CCTCATCTTG GATGAGATCT 
     2821 ATGGTGCTGT GGCCAAGTGG CTTACCAAAA TCGAGGTTCC GAAAACAGAA CAGACTTTTG 
     2881 AAGAACGGTT GATCCTCAAA GCTTTCTTGC TCAAGTTTGT CAATGCCTAC TCCCCCATCT 
     2941 TCTATGTGGC CTTTTTCAAG GGGAGGTTTG TGGGCAGACC TGGAAGCTAT GTCTACGTGT 
     3001 TCGACGGTTA CCGCATGGAA GAGTGTGCTC CGGGAGGCTG TCTCATGGAG CTGTGCATCC 
     3061 AACTCAGCAT CATCATGTTG GGCAAGCAGC TGATCCAGAA CAATATCTTT GAGATTGGAG 
     3121 TCCCGAAGCT AAAGAAACTC TTTCGGAAGC TGAAAGATGA GACAGAGCCT GGAGAAAGTG 
     3181 ACCCTGACCA CTCAAAACGT CCAGAGCAGT GGGACCTGGA CCACAGCCTA GAGCCTTACA 
     3241 CTGGGCTGAC TCCTGAGTAC ATGGAAATGA TCATCCAGTT TGGTTTTGTC ACCCTTTTTG 
     3301 TGGCCTCCTT TCCTCTGGCC CCTGTGTTTG CTCTCCTCAA CAATGTCATT GAAGTGCGGC 
     3361 TGGATGCAAA AAAGTTTGTT ACAGAACTGA GACGCCCAGA TGCTGTGAGG ACCAAAGACA 
     3421 TTGGAATTTG GTTTGACATT CTCTCTGGAA TTGGCAAGTT CTCCGTCATC ATTAATGCTT 
     3481 TTGTCATTGC TGTCACCTCC GACTTTATCC CCCGCCTTGT GTACCAGTAC TCCTATAGCC 
     3541 ACAACGGGAC ACTACATGGC TTTGTGAACC ATACACTCTC CTTTTTCAAC GTCAGTCAGC 
     3601 TGAAGGAGGG AACACAGCCT GAAAACTCAC AGTTCGACCA GGAGGTTCAG TTTTGCAGGT 
     3661 TCAAAGATTA TCGAGAACCA CCATGGGCAC CAAACCCTTA TGAGTTTTCC AAGCAGTACT 
     3721 GGTCTGTTTT GTCTGCCCGT CTGGCCTTTG TTATCATCTT CCAGAACCTA GTGATGTTCC 
     3781 TGAGTGTCCT GGTGGACTGG ATGATTCCGG ATATCCCCAC TGACATCAGC GACCAGATCA 
     3841 AGAAAGAGAA GAGCTTGCTG GTAGACTTCT TTCTGAAGGA GGAGCATGAG AAGGTCAAGC 
     3901 TGGCTGATGA GCCCACCCAG AGGAGCCAGG GAGGTGGGGA TCGGAGCAGG AGGAGCCGTG 
     3961 CAGCCAGCTC TGCACCATCA GGCCGGAGCC AGCCAGGCAG CATAGCATCC TCAGGGTCCC 
     4021 AGCACACCAA TGTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4081 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4141 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4201 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4261 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4321 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4381 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4441 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4501 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4561 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4621 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4681 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4741 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4801 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4861 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4921 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4981 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5041 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5101 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5161 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5221 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5281 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5341 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5401 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5461 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5521 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5581 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5641 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5701 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5761 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5821 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5881 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5941 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6001 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6061 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6121 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6181 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6241 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6301 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6361 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6421 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6481 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6541 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6601 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6661 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6721 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6781 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6841 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6901 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6961 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7021 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7081 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7141 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7201 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7261 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7321 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7381 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7441 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7501 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7561 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7621 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7681 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7741 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7801 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7861 ATTTAACAAA AATTTAACGC GAATTTT
//