LOCUS       MR219695                9465 bp    DNA     circular  SYN 
DEFINITION  Top2a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a)
ACCESSION   NM_011623
VERSION     NM_011623.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5622..5713 
                     /label="Myc-DDK"  
     misc_feature    5743..6328 
                     /label="PolyA signal"  
     misc_feature    5800..5819 
                     /label="Restriction Enzyme"  
     misc_feature    6604..7276 
                     /label="ColE1"  
     misc_feature    7706..8500 
                     /label="Kan/Neo"  
     misc_feature    8535..8837 
                     /label="SV40 ori"  
     misc_feature    8923..9400 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5604..5610 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5604 
                     /label="Top2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTTGTCA CCGCTGCAGC CTGTAAATGA AAATATGCTA ATGAACAAAA 
     1081 AGAAGAATGA AGATGGCAAG AAAAGATTGT CTATTGAAAG AATCTATCAG AAAAAAACAC 
     1141 AACTGGAACA TATACTGCTC CGCCCAGATA CCTACATTGG CTCTGTGGAG TTAGTGACCC 
     1201 AGCAAATGTG GGTTTACGAT GAAGATGTTG GCATTAACTA CAGGGAAGTC ACTTTTGTTC 
     1261 CTGGTTTGTA TAAAATCTTT GATGAGATTC TAGTTAATGC TGCTGATAAC AAACAAAGGG 
     1321 ACCCAAAAAT GTCTTGTATT AGAGTCACAA TTGATCCAGA AAATAATGTA ATTAGCATCT 
     1381 GGAATAATGG GAAAGGAATC CCTGTTGTTG AACACAAAGT TGAGAAAATA TATGTCCCAG 
     1441 CTCTCATATT TGGACAACTC CTGACCTCCA GTAACTATGA CGACGATGAG AAGAAAGTGA 
     1501 CAGGTGGTCG AAATGGCTAT GGAGCTAAAC TGTGTAACAT ATTCAGCACC AAATTTACTG 
     1561 TGGAAACAGC CAGTAGAGAG TACAAGAAAA TGTTCAAACA GACGTGGATG GATAACATGG 
     1621 GGAGAGCTGG TGACATGGAA CTCAAGCCCT TTAGTGGAGA AGATTATACA TGTATCACCT 
     1681 TCCAGCCTGA CTTATCTAAG TTTAAAATGC AAAGCCTGGA CAAAGATATT GTTGCACTGA 
     1741 TGGTCAGAAG AGCATATGAT ATCGCTGGCT CCACTAAAGA TGTCAAAGTC TTTCTTAATG 
     1801 GAAATAGTCT GCCAGTGAAA GGATTCCGCA GTTACGTGGA TTTGTATTTG AAGGATAAGG 
     1861 TAGATGAAAC TGGGAATTCA CTGAAGGTGA TACATGAACA GGTCAATCCC CGGTGGGAAG 
     1921 TGTGCTTAAC AATGAGCGAG AGAGGCTTCC AGCAGATTAG CTTCGTCAAC AGCATTGCGA 
     1981 CTTCTAAGGG CGGCAGACAT GTCGATTATG TAGCTGATCA GATTGTGAGC AAACTCGTTG 
     2041 ATGTGGTGAA AAAGAAGAAC AAGGGCGGGG TTGCAGTGAA GGCTCATCAG GTGAAAAATC 
     2101 ACATGTGGAT TTTTGTAAAT GCCTTAATTG AAAACCCAAC CTTTGACTCT CAAACAAAAG 
     2161 AAAACATGAC TTTACAAGCC AAGAGCTTTG GATCAACATG TCAATTAAGT GAAAAGTTCA 
     2221 TCAAAGCTGC AATTGGTTGT GGTATTGTGG AAAGCATACT AAACTGGGTG AAGTTCAAGG 
     2281 CTCAAATCCA ACTGAACAAG AAGTGTTCAG CTGTAAAACA TACCAAAATC AAGGGAATCC 
     2341 CCAAACTTGA TGATGCCAAC GATGCAGGGA GCCGAAACTC TACTGAATGC ACACTTATCC 
     2401 TGACTGAGGG AGACTCAGCA AAAACTCTAG CCGTTTCAGG CCTTGGAGTG GTTGGAAGAG 
     2461 ACAAATATGG GGTGTTCCCT CTTAGAGGAA AGATACTCAA TGTGCGAGAA GCCTCTCATA 
     2521 AACAGATCAT GGAAAATGCT GAAATTAACA ATATCATCAA GATTGTGGGT CTCCAGTACA 
     2581 AGAAAAACTA TGAAGATGAA GATTCATTGA AAACTCTTCG TTATGGGAAG ATCATGATTA 
     2641 TGACAGATCA GGACCAAGAT GGTTCCCACA TCAAAGGCTT GCTGATCAAT TTTATCCATC 
     2701 ACAATTGGCC ATCTCTTCTG CGACATCGTT TTCTAGAAGA ATTTATCACT CCCATTGTAA 
     2761 AGGTGTCCAA AAATAAGCAA GAAATAGCAT TCTATAGTCT TCCCGAGTTT GAAGAATGGA 
     2821 AAAGTTCGAC TCCGAATCAT AAAAAGTGGA AAGTCAAATA CTACAAAGGT TTGGGCACCA 
     2881 GCACATCAAA GGAAGCTAAG GAATATTTTG CAGATATGAA ACGACATCGT ATTCAGTTCA 
     2941 AATACTCTGG CCCTGAAGAT GATGCTGCGA TCAGCCTGGC CTTTAGTAAA AAACAAGTTG 
     3001 ATGATCGAAA GGAATGGTTA ACTAATTTTA TGGAAGACAG AAGACAGCGG AAGTTACTTG 
     3061 GCCTTCCTGA GGATTATTTG TATGGACAAA GCACTTCGTA CCTGACCTAT AATGACTTCA 
     3121 TAAACAAGGA ACTCATCCTC TTCTCCAACT CTGATAATGA AAGGTCTATC CCATCCATGG 
     3181 TAGACGGTTT GAAACCAGGT CAGAGAAAGG TTTTGTTTAC TTGTTTCAAA CGGAATGACA 
     3241 AGCGAGAAGT GAAGGTTGCC CAGTTAGCTG GGTCAGTGGC AGAAATGTCC TCTTATCACC 
     3301 ATGGTGAGAT GTCACTGATG ATGACCATTA TCAATTTGGC GCAGAATTTT GTGGGTAGCA 
     3361 ATAATTTGAA CCTTTTACAG CCCATTGGTC AGTTTGGAAC CAGGCTGCAT GGTGGCAAGG 
     3421 ACTCAGCTAG TCCTAGGTAC ATCTTTACAA TGCTCAGCCC TTTGGCTCGG TTGTTGTTTC 
     3481 CTCCAAAAGA TGATCACACA TTACGGTTTC TATATGATGA CAACCAACGT GTTGAGCCTG 
     3541 AATGGTACAT TCCTATAATA CCCATGGTGC TGATAAATGG TGCTGAGGGA ATTGGTACTG 
     3601 GGTGGTCCTG CAAAATCCCC AACTTTGATG TGCGTGAAGT TGTGAATAAT ATCAGACGGC 
     3661 TTCTGGATGG AGAGGAGCCC CTACCCATGC TCCCAAGTTA TAAGAATTTC AAAGGTACTA 
     3721 TTGAAGAACT GGCTTCAAAT CAGTATGTGA TTAATGGAGA AGTAGCTATT CTGGATTCTA 
     3781 CAACCATTGA AATCTCAGAG CTTCCCATCC GAACGTGGAC TCAGACATAT AAAGAACAGG 
     3841 TTCTAGAACC CATGTTGAAT GGAACTGAGA AGACACCCTC TCTAATAACA GACTATAGGG 
     3901 AATACCATAC AGATACCACT GTGAAGTTTG TCATAAAGAT GACTGAAGAA AAACTGGCAG 
     3961 AGGCAGAGAG AGTCGGCCTA CACAAAGTCT TCAAACTCCA GAGTAGTCTC ACTTGCAACT 
     4021 CTATGGTGCT TTTTGACCAT GTAGGTTGCT TAAAGAAATA TGACACTGTG TTGGATATTC 
     4081 TAAGAGACTT CTTTGAGCTC AGGCTTAAAT ATTATGGATT AAGAAAAGAA TGGCTTCTAG 
     4141 GAATGCTTGG TGCAGAATCT TCTAAACTGA ATAATCAAGC TCGCTTTATA TTAGAGAAAA 
     4201 TAGATGGCAA AATAGTCATT GAAAATAAAC CTAAAAAAGA ATTAATTAAA GTTCTGATTC 
     4261 AGAGAGGCTA CGACTCTGAC CCTGTGAAAG CCTGGAAAGA AGCTCAGCAG AAGGTTCCAG 
     4321 ATGAAGAAGA AAATGAAGAA AGTGACACTG AAACCAGCAC CAGTGACTCT GCAGCCGAGG 
     4381 CTGGGCCGAC CTTCAACTAC CTTCTCGACA TGCCCCTGTG GTATCTGACC AAGGAGAAGA 
     4441 AGGATGAGCT GTGCAAACAA AGAAACGAGA AGGAACAAGA GCTCAACACA TTAAAGCAAA 
     4501 AGAGTCCATC AGATCTGTGG AAGGAAGATC TGGCTGTTTT TATTGAAGAA CTGGAGGTTG 
     4561 TCGAGGCCAA GGAAAAGCAG GATGAACAAG TGGGCCTTCC TGGAAAGGCG GGGAAAGCAA 
     4621 AGGGGAAGAA AGCACAGATG TGTGCTGACG TCTTGCCTTC TCCTCGGGGC AAAAGAGTCA 
     4681 TCCCCCAAGT GACTGTGGAG ATGAAAGCTG AGGCAGAGAA GAAAATTAGG AAGAAAATTA 
     4741 AGAGTGAAAA CGTTGAGGGT ACCCCTGCTG AGGATGGTGC GGAACCAGGA AGCCTCCGGC 
     4801 AACGAATAGA GAAGAAGCAG AAGAAAGAGC CAGGTGCAAA GAAGCAGACT ACATTGCCGT 
     4861 TTAAGCCTGT CAAAAAAGGG AGGAAGAAAA ACCCCTGGTC TGACTCTGAG TCAGACGTGA 
     4921 GCAGTAATGA AAGTAACGTT GATGTCCCTC CTCGACAAAA AGAGCAAAGA AGTGCTGCAG 
     4981 CAAAAGCCAA ATTCACAGTG GATTTAGACT CGGATGAAGA TTTCTCAGGT TTGGATGAGA 
     5041 AGGATGAGGA TGAAGATTTC CTCCCATTAG ATGCTACTCC ACCTAAGGCC AAAATCCCCC 
     5101 CAAAAAATAC TAAAAAAGCA CTGAAGACCC AGGGAAGCTC CATGTCGGTA GTTGATCTTG 
     5161 AAAGTGATGT TAAGGACAGT GTGCCAGCTT CTCCAGGCGT TCCTGCTGCT GACTTCCCAG 
     5221 CGGAAACTGA ACAGTCAAAG CCATCCAAAA AGACCGTGGG AGTGAAGAAG ACAGCAACCA 
     5281 AAAGCCAGTC TTCAGTCTCC ACTGCTGGTA CCAAAAAGAG AGCTGCGCCA AAGGGAACCA 
     5341 AATCAGATTC AGCCTTGAGT GCTCGTGTCT CGGAAAAACC TGCTCCTGCC AAAGCCAAGA 
     5401 ACAGTCGCAA AAGGAAGCCA TCTTCTTCTG ATAGCTCTGA CTCTGATTTT GAGAGAGCAA 
     5461 TTTCTAAAGG TGCCACGAGC AAGAAAGCAA AGGGAGAGGA ACAGGACTTC CCTGTGGACT 
     5521 TGGAAGACAC GATAGCTCCT CGAGCCAAAT CTGACCGGGC GAGGAAGCCA ATTAAGTACC 
     5581 TGGAGGAGTC CGATGACGAT GACGACCTCT TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5641 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5701 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5761 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5821 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5881 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5941 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     6001 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     6061 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     6121 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6181 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6241 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6301 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6361 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6421 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6481 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6541 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6601 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6661 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6721 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6781 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6841 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6901 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6961 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     7021 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     7081 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     7141 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7201 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7261 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7321 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7381 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7441 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7501 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7561 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7621 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7681 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7741 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7801 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7861 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7921 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7981 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     8041 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     8101 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     8161 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8221 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8281 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8341 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8401 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8461 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8521 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8581 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8641 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8701 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8761 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8821 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8881 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8941 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9001 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     9061 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9121 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9181 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9241 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9301 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9361 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9421 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//