LOCUS       MR219556                9363 bp    DNA     circular  SYN 
DEFINITION  Rapgef2 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2)
ACCESSION   NM_001099624
VERSION     NM_001099624.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5520..5611 
                     /label="Myc-DDK"  
     misc_feature    5641..6226 
                     /label="PolyA signal"  
     misc_feature    5698..5717 
                     /label="Restriction Enzyme"  
     misc_feature    6502..7174 
                     /label="ColE1"  
     misc_feature    7604..8398 
                     /label="Kan/Neo"  
     misc_feature    8433..8735 
                     /label="SV40 ori"  
     misc_feature    8821..9298 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5502..5508 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5502 
                     /label="Rapgef2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTCCTT GTGCGTTGCT ATGCCAACTG CCTGCAGCCA TGGTCCTCCA 
     1081 AACTCCCTGC AGACTTCACA AAACTGCACC TCACTGACAG CCTGCACCCA CAGGTGACCC 
     1141 ACGTGTCCTC CAGCCATTCA GGATGTAGCA TCACCAGCGA CTCTGGGAGC AGCAGTCTTT 
     1201 CTGACATTTA CCAGGCCACG GAGAGCGAGG CCGGTGACAT GGACCTGAGT GGACTGCCAG 
     1261 AGACGGCGGT TGACTCTGAG GATGACGATG ATGAGGAAGA CATCGAGAGA GCATCAGACC 
     1321 CTCTGATGAG CAGGGACATC GTGAGGGACT GCCTGGAGAA GGACCCGATA GACAGGACTG 
     1381 ACGATGACAT CGAACAACTA CTGGAATTTA TGCACCAGCT GCCGGCCTTT GCCAATATGA 
     1441 CGATGTCAGT AAGACGGGAG CTCTGTGCCG TGATGGTGTT TGCAGTGGTG GAAAGAGCTG 
     1501 GAACCATTGT GTTAAACGAC GGAGAAGAGC TGGATTCCTG GTCAGTGATT CTCAATGGTT 
     1561 CCGTGGAGGT GACATATCCG GATGGGAAAG CAGAAATTCT GTGCATGGGA AACAGCTTTG 
     1621 GTGTTTCGCC CACCATGGAC AAAGAATACA TGAAAGGAGT CATGAGGACT AAGGTGGATG 
     1681 ACTGCCAGTT TGTCTGCATT GCCCAGCAAG ATTACTGCCG TATTTTGAAC CAAGTAGAAA 
     1741 AGAACATGCA AAAAGTTGAG GAGGAAGGAG AGATTGTAAT GGTGAAGGAG CACCGAGAAC 
     1801 TTGATCGGAC TGGAACAAGG AAGGGCCACA TCGTCATCAA GGGCACCTCA GAGAGGCTGA 
     1861 CCATGCATTT GGTGGAAGAG CATTCTGTAG TGGATCCAAC ATTCATAGAA GACTTCCTGT 
     1921 TGACATACCG GACTTTCCTT TCCAGCCCGA TGGAAGTGGG CAAGAAGTTA TTGGAGTGGT 
     1981 TCAATGACCC GAGCCTCAGG GATAAGGTGA CGCGGGTAGT ATTACTGTGG GTGAATAATC 
     2041 ACTTCAATGA CTTTGAAGGG GATCCTGCAA TGACTCGATT TCTGGAAGAA TTTGAAAATA 
     2101 ATCTGGAAAG AGAGAAAATG GGTGGACATC TAAGGCTGTT GAACATTGCA TGTGCAGCTA 
     2161 AAGCCAAACG AAGACTGATG ACGTTAACAA AGCCTTCCCG GGAAGCCCCT CTGCCCTTCA 
     2221 TCTTACTGGG AGGCTCTGAA AAGGGCTTTG GCATCTTCGT GGACAGCGTT GACTCGTGTA 
     2281 GCAAAGCCAC TGAAGCAGGC TTGAAGCGCG GGGATCAGAT ATTAGAAGTG AATGGTCAAA 
     2341 ATTTTGAAAA TATCCAACTT TCAAAGGCAA TGGAAATTCT CAGAAATAAC ACACACCTGT 
     2401 CCATCACTGT GAAAACCAAC TTATTTGTAT TTAAAGAACT TCTGACGAGA TTGTCTGAAG 
     2461 AGAAAAGAAA CGGTGCCCCT CACCTCCCTA AAATCGGGGA CATCAAGAAG GCCAGCCGCT 
     2521 ACTCCATACC GGATCTCGCC GTAGACGTAG AACAGGTGAT AGGCCTCGAG AAGGTCAACA 
     2581 AGAAAAGCAA AGCCAACACC GTGGGAGGAA GAAACAAGCT GAAGAAAATA CTCGACAAGA 
     2641 CCCGGATCAG CATCTTACCT CAGAAACCAT ACAATGATAT TGGGATTGGT CAGTCTCAAG 
     2701 ATGATAGCAT CGTAGGACTG AGGCAGACCA AGCACATCCC AGCTGCCCTG CCTGTCAGTG 
     2761 GAACGCTGTC CTCCAGTAAC CCTGACTTGC TGCAGTCACA CCACCGGATT CTGGACTTCA 
     2821 GCACTACTCC TGACTTGCCA GATCAGGTAC TGAGAGTTTT CAAGGCTGAC CAACAAAGCA 
     2881 GGTACATCAT GATCAGCAAG GACACCACCG CAAAGGAAGT GGTCATCCAG GCCATCAGAG 
     2941 AGTTTGCTGT CACTGCCACC CCAGAGCAGT ATTCCCTCTG TGAGGTCTCA GTCACACCAG 
     3001 AGGGAGTTAT CAAACAACGC CGACTCCCAG ATCAGCTTTC CAAGCTTGCC GACAGGATAC 
     3061 AACTGAGTGG AAGGTATTAT CTGAAAAACA ACATGGAAAC AGAAACGCTT TGCTCAGATG 
     3121 AAGATGCCCA GGAGCTGCTG CGAGAGAGCC AAATCTCCCT CCTTCAGCTC AGCACCGTGG 
     3181 AGGTTGCCAC GCAGCTCTCC ATGAGGAACT TTGAACTCTT CCGCAACATT GAGCCTACTG 
     3241 AATACATAGA TGATTTATTT AAACTCAAAT CAAAAACCAG CTGTGCCAAC CTGAAGAAAT 
     3301 TTGAGGAAGT CATTAACCAG GAAACATTTT GGGTGGCTTC TGAAATCCTG CGGGAAACAA 
     3361 ACCAGCTGAA GAGGATGAAG ATCATTAAGC ATTTCATCAA GATTGCCTTG CACTGCAGGG 
     3421 AATGCAAGAA TTTTAACTCC ATGTTTGCAA TCATCAGTGG CCTAAACCTG GCACCGGTGG 
     3481 CAAGACTACG AACCACCTGG GAAAAGCTTC CCAATAAATA TGAAAAGCTG TTCCAGGACC 
     3541 TGCAAGACCT GTTTGATCCT TCCAGAAACA TGGCAAAGTA CCGCAATGTC CTGAGTGGCC 
     3601 AGAACTTACA GCCTCCTGTC ATTCCCCTGT TCCCTGTCAT CAAGAAGGAC CTCACGTTTC 
     3661 TCCATGAGGG AAATGACTCA AAAGTAGACG GACTGGTCAA CTTTGAGAAG CTGAGGATGA 
     3721 TTGCAAAAGA AATCCGTCAC GTTGGCCGAA TGGCATCAGT CAACATGGAC CCTGCCCTTA 
     3781 TGTTCAGGAC TCGGAAGAAG AAGTGGCGGA GTCTGGGGTC CCTGAGTCAG GGCAGTGCAA 
     3841 ACGCCACAGT GCTGGATGTT GCTCAGACAG GCGGGCATAA AAAGCGGGTA CGCCGTAGTT 
     3901 CCTTCCTCAA TGCCAAAAAG CTGTATGAAG ATGCCCAGAT GGCTCGGAAA GTGAAGCAAT 
     3961 ACCTGTCCAA CCTGGAGCTG GAGATGGATG AGGAGAGTCT TCAGACACTG TCACTGCAGT 
     4021 GTGAACCAGC AACCAGCACA TTGCCCAAGA ATCCTGGAGA CAAAAAGCCT GTCAAGTCTG 
     4081 AGACCTCCCC AGTGGCTCCA CGTGCAGGGC CCCAGCAGAA AGTACAGCCT CAGCAGCCGC 
     4141 TGGCACAGCC ACAACCTCCA CATAAAGTCA GCCAGGGACT GCAGGTGCCC GCTGTGTCCC 
     4201 TCTATCCTTC ACGAAAGAAG GTGCCGGTGA AGGATCTGCC TCCTTTTGGC ATAAACTCTC 
     4261 CACAAGCTTT GAAGAAAATC CTGTCCTTGT CTGAAGAAGG GAGTTTGGAG CGTCACAGGA 
     4321 AGCAAGCAGA AGATACAATA TCAAATGCGT CCTCTCAGCT CTCATCGCCC CCCACCTCAC 
     4381 CTCAGAGCTC TCCAAGGAAA GGTTATGCAC TGGCTCTCAG TGGCACCGTG GATAATTTCT 
     4441 CAGATTCAGG TCACAGTGAG ATTTCTTCAC GATCCAGTAT TGTCAGCAAT TCTTCTTTTG 
     4501 ACTCGGTGCC AGTCTCTCTG CATGATGAAC GGCGCCAGAG GCATTCTGTC AGCATTGTGG 
     4561 AGAGCAACCT AGGTGTGGGC AGGATGGAGC GGCGGACCCT GATGGAGCCT GATCAATACA 
     4621 GCTTAGGGTC GTATGCCCCA GTGTCTGAAA GCCGAGGCCT GTATGCCGCA GCGACGGTCA 
     4681 TCTCTTCTCC TAGCACAGAG GAGCTTTCCC ATGACCAAGG GGACCGTGCT TCCCTCGATG 
     4741 CTGCTGACAG CGGTCGTGGG AGCTGGACAT CATGCTCAAG TGGTTCCCAT GACAACATAC 
     4801 AGACCATTCA GCACCAGAGA AGCTGGGAAA CACTGCCATT TGGGCACACT CACTTTGACT 
     4861 ATTCAGGGGA TGCTGCAAGT ATATGGGCCT CCGGTGGCCA TATGGACCAA ATGATGTTTT 
     4921 CTGATCATAG CACAAAATAT AACAGACAGA ATCAAAGTAG AGAGAGTCTT GAACAAGCCC 
     4981 AATCCCGGGC CAGCTGGGCG TCTTCCACGG GCTACTGGGG AGAAGACTCA GAAGGCGACA 
     5041 CAGGCACTAT CAAGCGAAGG GGTGGGAAGG ATGTCTCCGC TGAGGCAGAG AGCAGCAGCA 
     5101 TGGTGCCCGT GACTACAGAG GAAGCCAAAC CTGTCCCTAT GCCTGCCCAC ATAGCTGTGA 
     5161 CGCCGAGCAC TACCAAGGGA CTCATCGCAC GGAAGGAAGG CAGGTACCGG GAGCCGCCTC 
     5221 CCACACCTCC AGGCTACGTG GGCATCCCCA TTGCCGATTT CCCAGAAGGG CCTTGCCACC 
     5281 CGGCCAGGAA GCCCCCGGAT TACAACGTGG CCCTGCAGCG GTCCCGCATG GTGGCACGGC 
     5341 CCACTGAGGC CCCGGCACCG GGCCAGACGC CGCCTGCAGC CGCAGCCAGC CGGCCGGGCA 
     5401 GCAAGCCACA GTGGCACAAG CCCAGCGACG CAGACCCACG CCTCGCGCCC TTCCAGCCGC 
     5461 AGGGCTTCGC AGGAGCGGAG GAGGACGAAG ATGAACAAGT GTCTGCTGTT ACGCGTACGC 
     5521 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5581 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5641 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5701 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5761 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5821 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5881 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5941 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     6001 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6061 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6121 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6181 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6241 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6301 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6361 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6421 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6481 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6541 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6601 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6661 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6721 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6781 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6841 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6901 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6961 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     7021 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7081 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7141 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7201 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7261 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7321 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7381 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7441 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7501 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7561 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7621 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7681 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7741 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7801 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7861 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7921 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7981 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     8041 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8101 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8161 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8221 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8281 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8341 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8401 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8461 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8521 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8581 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8641 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8701 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8761 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8821 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8881 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8941 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     9001 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9061 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9121 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9181 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9241 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9301 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9361 TTT
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