LOCUS       MR218778                9030 bp    DNA     circular  SYN 
DEFINITION  Pask (Myc-DDK-tagged) - Mouse PAS domain containing serine/threonine kinase (Pask)
ACCESSION   NM_080850
VERSION     NM_080850.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5187..5278 
                     /label="Myc-DDK"  
     misc_feature    5308..5893 
                     /label="PolyA signal"  
     misc_feature    5365..5384 
                     /label="Restriction Enzyme"  
     misc_feature    6169..6841 
                     /label="ColE1"  
     misc_feature    7271..8065 
                     /label="Kan/Neo"  
     misc_feature    8100..8402 
                     /label="SV40 ori"  
     misc_feature    8488..8965 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5169..5175 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5169 
                     /label="Pask"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGACAGA GGTCCACCAG TTTTTGCTGA GGACTGGAAA TGTTTGTCTG 
     1081 AGAGTCCTCC TGTCCAAGAA GGCCCAGCGG CCCAGGCTAC CTTTGAGCCC AGCAAGCCAC 
     1141 TCTCCATAGC CCACAAACAC CTGAGCAGGA AGAATGGGCT TTCTAGACTC TGCCAGAGCC 
     1201 GAATGGCGCT CTCTGAGGAC AGGTGGAGCT CTTACTGTCT TTCATCATTG GCTGCCCAAA 
     1261 ACATCTGCAC AAGCAAACTG CACTGCGCTG CTGCACCTGA GTATGCTGAC CCTACAGCTG 
     1321 GGCCACTGGG CAGTACTTCC TGCTGCTCCT TGCTGCGTGG CCTGGCTTCA GGATGCTCTG 
     1381 GGTCCCTGCT TTCGACCCCT GTGTGCAACC CCAACAAGGC TGTCTTTACC GTGGATGCCA 
     1441 AGACCACAGA GATTCTGGTT GCCAACGACA AAGCCTGCAG CCTCCTAGGG TATAGCAGTC 
     1501 ATGACCTGAT TGGCCAAAAG CTCGCCCAAT TCTTCCTCAA ATCTGATTCC GAGGTGGTGG 
     1561 AAGCCCTCAG CGAGGAACAT GTGGAGGCTG ATGGCCACGC TGCAGTGGTG TTCGGCACAG 
     1621 TGGTGGACAT CGTCAGCCGA ATTGGGGAGA AGATTCCAGT CTCTGTGTGG ATAAAGAGAT 
     1681 TGCAGCAAGA TCGTGGCTTG TGCTGTGTAG TGGTTCTGGA GCCTGTGGAG AGAGTGTCAG 
     1741 CCTGGGTCGC CTTCCAGAGT GATGGAACTA TCACATCATG CGACAGTCTC TTTGCTCATC 
     1801 TCCATGGGTT CACATCTCCA AAGGATGTAG TTGGGCAGTG TGTCATAGAC TTGATTCCAT 
     1861 CCATGCAGCT CCCTCCTCCT GGCCAACATA TCCCAAAGAG TCTCAAGATT CAAAGGTCTG 
     1921 TTGGGAGAGC CAGAGACGGC ACCACCTTCC CTCTCAGTTT GAAGCTGAAA TCCAAGCCCA 
     1981 GTGGAAGGGC TGTAGCTGAC AGTGAGGCGG CCTCTGAACC AGGTTACCAG GCTTCTGTGT 
     2041 GGGTGTTCTG CACCATCAGT GGCCTCATCA CTCTGCTGCC AGATGGCACA ATTTATGGAG 
     2101 TCAACCACAG CTTTGCACTA ATGCTGTTTG GTTATGGAAA GACAGAGCTC CTGGGCAAGA 
     2161 ATATCACTTT CCTGATTCCT GGTTTCTACC ACTACATGGA CCTCACATAT GATAGTTCGG 
     2221 TACAGCTGCC AGATCTGGTC AATTGCCTCG ACATTGGCAG AAAGAGTGGG CCTGGGGAGA 
     2281 TGAACTCTGA TGCCCAACAC AACTGGGAAC TGGCCAGTGG GGCCCAGGGT CCAAGAATTG 
     2341 ACGTGGTGTT GGCACGGGAC CACATGCCAT CACAAGATGA GACCCTGAAG CTGGTGGGAG 
     2401 GACAAGTCTC CTCTAGGACT CAGACACGAT TGGAAACTGG ATACAAGATA CTTCCTTCTT 
     2461 CTGCATGTCA GCCCTCACTG GGGGTGGACA GCAACCCAGA GGATGGTGAG CAGTCATTGC 
     2521 TCACAGACCA GCAAAGCATT CCAAAGAGAA ACCTGCCAGC CCATGGTGGT CAGAACCAGC 
     2581 TAGACACCTC AGAAATATCC CTACCAGTCC TTAAAGAACA TTTATTGTCT GAAATTCAGA 
     2641 AAAATATCTC AGAAGAAAGC CCACTAACCC ACAGGAAATG GTTATCCAAG GTGCAACAAA 
     2701 ACCCCACAAA AGGAAGCCTG CCCATCCACG AGGAGCAGCT ATTGTTTGCA GGCCAGCATA 
     2761 TCCATGTCTT GGGAAAGGAG GATCCTTCAG CAGCAGAGAG CTACAGGGAG AGTCTTTTAG 
     2821 AAGAAAGCAA GTCCAAACCA GTGGATGCAA AACTGTTTGC TTCATGTGAA GATTCTGAGC 
     2881 CTCTAGTTTC AGTTAAGGAC AGGGGCAGCA GTGTGGACAC GTGTAATCTG CATCAGGAAG 
     2941 CTCAGTTGGA GCTGATGGGT GTCAGCAGTC CTAACCCCTG GGCTGATGCC ACCATGCCTG 
     3001 AGCCCCACAC CACAGGGCAG ATAGCAGGCG GGAGCCTTAC ATATTGCCCA CAGTACAGAA 
     3061 GCGAGTGGGC CTCACAGCAG CGAGGCCAGG ACTCGGCTCC CAGCCCCTCT GGAATGGCGT 
     3121 GTGTCTTGCT TGGGACACCC ACTCTGGATG AACCATGGCC AGGAGTGAGA AATGACAGAG 
     3181 AAGAGCTGCA GACTTGCTTG ATTAAGGAAC AGTTATCCAA GTCAAGCTGT GAGGGAAATC 
     3241 TGGGCATCTC CCGTGTGGAA CTCGTTCCTG AAGAACATCC TCCTTTCACC GCCCCCGTGT 
     3301 CTTTCTGTGA TCTGGGAGGC CGAGATCTAC ACGCTAGCCG CTCAGGCAGC TCCTCAGCCT 
     3361 GCTATGCTTT GGCCACCGAC CTCCCTGGTG TCCTGGAGGC AGTGGAAGCT CAGGAGGCTG 
     3421 ATGTGAATTC TTATTCCTGG AACCTCAAGG AGCTCTTTCT CAAGGACCAG ACAGACAGAA 
     3481 CTCCATCACA TTGTTCCTGT ACCACATCTG AACTCAGCGA GGCGCCATCT CTTTCAGTGG 
     3541 TGGGGTCAGA TTTAGATGTG GGTATTCTGC ATAGGCAGAC TTCAGATATT TTAGTTGACC 
     3601 GAGAGATGTT GCTCCTTACT GGGACCTATT TTGACCTTAG TGAAGGCCAA CGGTTCCAGG 
     3661 AGATGGGAGC AGGACATGAT CGAGCAGAAC TGTCTAACAT TTCTTTGGTA TCCTCTGAAC 
     3721 ACTATGAAAC TAGTGACATA GAAAGCCCAG GTTGTGACCC TCCCTTACCA GATCCTGGTC 
     3781 CCAACGATAT GTGCCTATCA GCAGAGAAGC CCAGGCCGAG TGCCCAGATC ACTTCCACTC 
     3841 CTGTGGCACG TGGGGCCACC AGCCTGCAGC AGGAGATCCA AGAGGGCATC TACTCTGGAA 
     3901 GCTGCTACCA CCGGGATGGC TTACAACTGA GTATACAGTT TGAAGTCAAG CGTGTGGAGC 
     3961 TCCAGGGCTC TGCGACCCTG TTCTGCTGCT GGCTGGTGAA GGACCTCTTC CATAGCCACC 
     4021 GGGACTCAGC CACACGAACC CGCCTGTTCC TTGCCAGTCT GCCTAGCTCT ACCCACTCCA 
     4081 TGCCTGAGCT GTCTGGATCC AGCTTTGGAG AGGTGCTCAG AGCCAAGCCT TGGTTTGAGG 
     4141 AATCCCCAAC ACCTGCAGAG CTAGAGGGGC TGGCGGCCTG TGAGGGCGAG TACGACTATA 
     4201 AGTATAACAC CATCAGCCCA CTTGGCAGTG GGGCCTTTGG CTTTGTGTGG ACTGCAGTGG 
     4261 AGAAGGAATG CAACAAAGAG GTGGTGGTGA AGTTTATTAA GAAGGAGAAG GTTTTAGAGG 
     4321 ATTGCTGGAT TGAGGATCCC AAACTTGGAA GAGTTACTTT AGAGATTGCA ATTCTGTCCA 
     4381 AGGTGGACCA TGCCAACATA ATCAAGGTTC TGGATATATT CGAAAACCAA GAGTTCTTCC 
     4441 AGCTGGTGAT GGAAAAGCAC GGCTCTGGCA TGGACCTCTT TGCTTTCATT GACCACCATC 
     4501 CCTGCTTGGA TGAGCCCCTG GCGAGTTTCA TCTTTCGACA ACTAGTGTCT GCTGTAGGAT 
     4561 ACCTGCACTC CCAAGGCATC ATCCATAGAG ACATCAAGGA TGAGAACATT GTGATTGCTG 
     4621 AGGACTTCAC AATTAAGCTG ATAGATTTTG GCTCAGCTGC CTACTTAGAG AGGGGCAAAC 
     4681 TATTTTATAC CTTTTGTGGA ACAATCGAAT ACTGTGCACC TGAGGTTCTC ATTGGAAATC 
     4741 CCTACAGAGG GCCAGAGCTG GAGATGTGGT CTCTGGGGGT CACCCTGTAC ACGCTCATCT 
     4801 TCGAGGAGAA TCCCTTCTGT GAGGTGGAGG AGACCATGGA GGCAGTTATT CATCCCCCAT 
     4861 TCCTGGTTTC CCAAGAACTT ATGAGTCTTC TGTCTGGACT GCTGCAGCCT TGCCCTGAGC 
     4921 AGCGGACCAC TTTGGAGAAG CTGATCAGGG ACCCCTGGGT GACACAGCCT GTGAACCTTG 
     4981 CTAGCTATAC TTGGGAAGAG GTGTGTAGGA CCAACCAGCC AGAAAGTGGC CTGCTGTCAG 
     5041 CTGCAAGTCT GGAGATTGGG AGTAGGAGTC CAAGTGAAAT GGCTCAGAGA GAGGGTCTCT 
     5101 GTGGGCCTCC TGCTCCCAGG GAGACTCGTG GTGACCAGCA CTGCTTGCAT CTTAAGGACC 
     5161 CCTCTTTGCC AGTCAGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5221 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5281 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5341 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5401 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5461 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5521 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5581 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5641 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5701 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5761 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5821 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5881 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5941 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6001 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6061 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6121 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6181 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6241 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6301 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6361 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6421 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6481 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6541 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6601 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6661 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6721 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6781 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6841 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6901 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6961 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7021 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7081 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7141 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7201 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7261 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7321 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7381 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7441 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7501 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7561 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7621 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7681 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7741 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7801 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7861 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7921 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7981 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8041 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8101 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8161 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8221 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8281 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8341 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8401 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8461 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8521 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8581 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8641 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8701 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8761 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8821 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8881 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8941 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9001 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//